No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 375 |
| AciI | CCGC | 8 cut(s) | 15, 38, 48, 135, 158, 339, 362, 373 |
| AclWI | GGATC | 1 cut(s) | 48 |
| AcoI | YGGCCR | 1 cut(s) | 228 |
| AcsI | RAATTY | 1 cut(s) | 245 |
| AcuI | CTGAAG | 1 cut(s) | 278 |
| AfaI | GTAC | 2 cut(s) | 111, 216 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 335, 461 |
| AgsI | TTSAA | 2 cut(s) | 118, 243 |
| AjnI | CCWGG | 1 cut(s) | 314 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 251, 283 |
| AluBI | AGCT | 3 cut(s) | 26, 148, 256 |
| AluI | AGCT | 3 cut(s) | 26, 148, 256 |
| Alw21I | GWGCWC | 1 cut(s) | 314 |
| AlwI | GGATC | 1 cut(s) | 48 |
| AoxI | GGCC | 4 cut(s) | 207, 228, 386, 519 |
| ApoI | RAATTY | 1 cut(s) | 245 |
| AseI | ATTAAT | 2 cut(s) | 84, 96 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 429 |
| AspA2I | CCTAGG | 1 cut(s) | 416 |
| AspLEI | GCGC | 2 cut(s) | 198, 321 |
| AspS9I | GGNCC | 4 cut(s) | 127, 160, 452, 519 |
| AsuC2I | CCSGG | 1 cut(s) | 518 |
| AvaII | GGWCC | 3 cut(s) | 127, 160, 452 |
| AvrII | CCTAGG | 1 cut(s) | 416 |
| BalI | TGGCCA | 1 cut(s) | 230 |
| Bbv12I | GWGCWC | 1 cut(s) | 314 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BccI | CCATC | 2 cut(s) | 83, 452 |
| BciT130I | CCWGG | 1 cut(s) | 316 |
| BcnI | CCSGG | 1 cut(s) | 518 |
| BfaI | CTAG | 5 cut(s) | 369, 390, 417, 500, 523 |
| BisI | GCNGC | 3 cut(s) | 135, 362, 373 |
| BlnI | CCTAGG | 1 cut(s) | 416 |
| BlsI | GCNGC | 3 cut(s) | 136, 363, 374 |
| Bme1390I | CCNGG | 2 cut(s) | 316, 518 |
| Bme18I | GGWCC | 3 cut(s) | 127, 160, 452 |
| BmgT120I | GGNCC | 4 cut(s) | 127, 160, 452, 519 |
| BmiI | GGNNCC | 1 cut(s) | 453 |
| BmrFI | CCNGG | 2 cut(s) | 316, 518 |
| BmsI | GCATC | 1 cut(s) | 480 |
| BpmI | CTGGAG | 1 cut(s) | 503 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 1 cut(s) | 518 |
| BsaJI | CCNNGG | 3 cut(s) | 210, 396, 416 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 338, 368 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 335, 461 |
| Bse118I | RCCGGY | 1 cut(s) | 335 |
| Bse1I | ACTGG | 1 cut(s) | 486 |
| BseBI | CCWGG | 1 cut(s) | 316 |
| BseDI | CCNNGG | 3 cut(s) | 210, 396, 416 |
| BseGI | GGATG | 1 cut(s) | 325 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 335, 461 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseNI | ACTGG | 1 cut(s) | 486 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BshFI | GGCC | 4 cut(s) | 209, 230, 388, 521 |
| BsiHKAI | GWGCWC | 1 cut(s) | 314 |
| BsiSI | CCGG | 4 cut(s) | 125, 336, 446, 517 |
| BslFI | GGGAC | 2 cut(s) | 398, 465 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 335, 461 |
| BsmFI | GGGAC | 2 cut(s) | 398, 465 |
| BsnI | GGCC | 4 cut(s) | 209, 230, 388, 521 |
| Bsp1286I | GDGCHC | 1 cut(s) | 314 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| BspACI | CCGC | 8 cut(s) | 15, 38, 48, 135, 158, 339, 362, 373 |
| BspANI | GGCC | 4 cut(s) | 209, 230, 388, 521 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspLI | GGNNCC | 1 cut(s) | 453 |
| BspPI | GGATC | 1 cut(s) | 48 |
| BsrBI | CCGCTC | 1 cut(s) | 375 |
| BsrFI | RCCGGY | 1 cut(s) | 335 |
| BsrI | ACTGG | 1 cut(s) | 486 |
| BssAI | RCCGGY | 1 cut(s) | 335 |
| BssECI | CCNNGG | 3 cut(s) | 210, 396, 416 |
| BssMI | GATC | 1 cut(s) | 40 |
| BssT1I | CCWWGG | 1 cut(s) | 416 |
| Bst2UI | CCWGG | 1 cut(s) | 316 |
| Bst6I | CTCTTC | 1 cut(s) | 254 |
| BstC8I | GCNNGC | 2 cut(s) | 299, 386 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 459 |
| BstF5I | GGATG | 1 cut(s) | 325 |
| BstHHI | GCGC | 2 cut(s) | 198, 321 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 272, 298, 318, 381 |
| BstNI | CCWGG | 1 cut(s) | 316 |
| BstSCI | CCNGG | 2 cut(s) | 314, 516 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 481 |
| BsuRI | GGCC | 4 cut(s) | 209, 230, 388, 521 |
| BtsCI | GGATG | 1 cut(s) | 325 |
| Cac8I | GCNNGC | 2 cut(s) | 299, 386 |
| CfoI | GCGC | 2 cut(s) | 198, 321 |
| Cfr10I | RCCGGY | 1 cut(s) | 335 |
| Cfr13I | GGNCC | 4 cut(s) | 127, 160, 452, 519 |
| Csp6I | GTAC | 2 cut(s) | 110, 215 |
| CviQI | GTAC | 2 cut(s) | 110, 215 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| EaeI | YGGCCR | 1 cut(s) | 228 |
| Eam1104I | CTCTTC | 1 cut(s) | 254 |
| EarI | CTCTTC | 1 cut(s) | 254 |
| EciI | GGCGGA | 2 cut(s) | 37, 354 |
| Eco130I | CCWWGG | 1 cut(s) | 416 |
| Eco147I | AGGCCT | 1 cut(s) | 388 |
| Eco47I | GGWCC | 3 cut(s) | 127, 160, 452 |
| Eco57I | CTGAAG | 1 cut(s) | 278 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 459 |
| EcoO109I | RGGNCCY | 1 cut(s) | 452 |
| EcoRII | CCWGG | 1 cut(s) | 314 |
| EcoT14I | CCWWGG | 1 cut(s) | 416 |
| ErhI | CCWWGG | 1 cut(s) | 416 |
| FaiI | YATR | 6 cut(s) | 140, 144, 366, 407, 443, 476 |
| FaqI | GGGAC | 2 cut(s) | 398, 465 |
| FauI | CCCGC | 1 cut(s) | 8 |
| Fnu4HI | GCNGC | 3 cut(s) | 135, 362, 373 |
| FokI | GGATG | 1 cut(s) | 312 |
| Fsp4HI | GCNGC | 3 cut(s) | 135, 362, 373 |
| FspBI | CTAG | 5 cut(s) | 369, 390, 417, 500, 523 |
| GlaI | GCGC | 2 cut(s) | 197, 320 |
| GluI | GCNGC | 3 cut(s) | 135, 362, 373 |
| GsuI | CTGGAG | 1 cut(s) | 503 |
| HaeIII | GGCC | 4 cut(s) | 209, 230, 388, 521 |
| HapII | CCGG | 4 cut(s) | 125, 336, 446, 517 |
| HhaI | GCGC | 2 cut(s) | 198, 321 |
| Hin6I | GCGC | 2 cut(s) | 196, 319 |
| HinP1I | GCGC | 2 cut(s) | 196, 319 |
| HincII | GTYRAC | 1 cut(s) | 103 |
| HindII | GTYRAC | 1 cut(s) | 103 |
| HinfI | GANTC | 2 cut(s) | 353, 430 |
| HpaI | GTTAAC | 1 cut(s) | 103 |
| HpaII | CCGG | 4 cut(s) | 125, 336, 446, 517 |
| Hpy166II | GTNNAC | 1 cut(s) | 103 |
| Hpy188III | TCNNGA | 1 cut(s) | 179 |
| Hpy8I | GTNNAC | 1 cut(s) | 103 |
| HpyAV | CCTTC | 1 cut(s) | 173 |
| HpyCH4V | TGCA | 1 cut(s) | 493 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 272, 298, 318, 381 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HspAI | GCGC | 2 cut(s) | 196, 319 |
| KspAI | GTTAAC | 1 cut(s) | 103 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LmnI | GCTCC | 2 cut(s) | 193, 484 |
| LweI | GCATC | 1 cut(s) | 480 |
| MaeI | CTAG | 5 cut(s) | 369, 390, 417, 500, 523 |
| MaeIII | GTNAC | 1 cut(s) | 354 |
| MalI | GATC | 1 cut(s) | 42 |
| MbiI | CCGCTC | 1 cut(s) | 375 |
| MboI | GATC | 1 cut(s) | 40 |
| MboII | GAAGA | 1 cut(s) | 271 |
| MhlI | GDGCHC | 1 cut(s) | 314 |
| MlsI | TGGCCA | 1 cut(s) | 230 |
| MluCI | AATT | 1 cut(s) | 245 |
| MluNI | TGGCCA | 1 cut(s) | 230 |
| MlyI | GAGTC | 1 cut(s) | 362 |
| MmeI | TCCRAC | 1 cut(s) | 249 |
| MnlI | CCTC | 7 cut(s) | 22, 205, 255, 340, 391, 442, 455 |
| Mox20I | TGGCCA | 1 cut(s) | 230 |
| MroXI | GAANNNNTTC | 1 cut(s) | 429 |
| MscI | TGGCCA | 1 cut(s) | 230 |
| MseI | TTAA | 3 cut(s) | 84, 96, 102 |
| Msp20I | TGGCCA | 1 cut(s) | 230 |
| MspI | CCGG | 4 cut(s) | 125, 336, 446, 517 |
| MspR9I | CCNGG | 2 cut(s) | 316, 518 |
| MvaI | CCWGG | 1 cut(s) | 316 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 272, 298, 318, 381 |
| NciI | CCSGG | 1 cut(s) | 518 |
| NdeII | GATC | 1 cut(s) | 40 |
| NlaIV | GGNNCC | 1 cut(s) | 453 |
| NmeAIII | GCCGAG | 1 cut(s) | 235 |
| NmuCI | GTSAC | 1 cut(s) | 354 |
| PceI | AGGCCT | 1 cut(s) | 388 |
| PdmI | GAANNNNTTC | 1 cut(s) | 429 |
| PfeI | GAWTC | 1 cut(s) | 430 |
| PkrI | GCNGC | 3 cut(s) | 136, 363, 374 |
| PleI | GAGTC | 1 cut(s) | 361 |
| PpsI | GAGTC | 1 cut(s) | 361 |
| PpuMI | RGGWCCY | 1 cut(s) | 452 |
| PshBI | ATTAAT | 2 cut(s) | 84, 96 |
| Psp5II | RGGWCCY | 1 cut(s) | 452 |
| Psp6I | CCWGG | 1 cut(s) | 314 |
| PspGI | CCWGG | 1 cut(s) | 314 |
| PspN4I | GGNNCC | 1 cut(s) | 453 |
| PspPI | GGNCC | 4 cut(s) | 127, 160, 452, 519 |
| PspPPI | RGGWCCY | 1 cut(s) | 452 |
| RsaI | GTAC | 2 cut(s) | 111, 216 |
| RsaNI | GTAC | 2 cut(s) | 110, 215 |
| SaqAI | TTAA | 3 cut(s) | 84, 96, 102 |
| SatI | GCNGC | 3 cut(s) | 135, 362, 373 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 4 cut(s) | 127, 160, 452, 519 |
| SchI | GAGTC | 1 cut(s) | 362 |
| ScrFI | CCNGG | 2 cut(s) | 316, 518 |
| SduI | GDGCHC | 1 cut(s) | 314 |
| SfaNI | GCATC | 1 cut(s) | 480 |
| SgrAI | CRCCGGYG | 1 cut(s) | 335 |
| SinI | GGWCC | 3 cut(s) | 127, 160, 452 |
| Sse9I | AATT | 1 cut(s) | 245 |
| SseBI | AGGCCT | 1 cut(s) | 388 |
| SsiI | CCGC | 8 cut(s) | 15, 38, 48, 135, 158, 339, 362, 373 |
| SspMI | CTAG | 5 cut(s) | 369, 390, 417, 500, 523 |
| StuI | AGGCCT | 1 cut(s) | 388 |
| StyD4I | CCNGG | 2 cut(s) | 314, 516 |
| StyI | CCWWGG | 1 cut(s) | 416 |
| TaqI | TCGA | 1 cut(s) | 178 |
| TasI | AATT | 1 cut(s) | 245 |
| TauI | GCSGC | 3 cut(s) | 137, 364, 375 |
| TfiI | GAWTC | 1 cut(s) | 430 |
| Tru1I | TTAA | 3 cut(s) | 84, 96, 102 |
| Tru9I | TTAA | 3 cut(s) | 84, 96, 102 |
| TseFI | GTSAC | 1 cut(s) | 354 |
| Tsp45I | GTSAC | 1 cut(s) | 354 |
| VpaK11BI | GGWCC | 3 cut(s) | 127, 160, 452 |
| VspI | ATTAAT | 2 cut(s) | 84, 96 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 459 |
| XapI | RAATTY | 1 cut(s) | 245 |
| XmaJI | CCTAGG | 1 cut(s) | 416 |
| XmnI | GAANNNNTTC | 1 cut(s) | 429 |
| XspI | CTAG | 5 cut(s) | 369, 390, 417, 500, 523 |