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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 509 |
| Acc16I | TGCGCA | 1 cut(s) | 323 |
| AccBSI | CCGCTC | 1 cut(s) | 333 |
| AccI | GTMKAC | 1 cut(s) | 187 |
| AciI | CCGC | 6 cut(s) | 20, 308, 331, 365, 394, 442 |
| AclWI | GGATC | 2 cut(s) | 430, 443 |
| AcoI | YGGCCR | 1 cut(s) | 90 |
| AcsI | RAATTY | 1 cut(s) | 107 |
| AcuI | CTGAAG | 1 cut(s) | 140 |
| AcyI | GRCGYC | 1 cut(s) | 561 |
| AfaI | GTAC | 1 cut(s) | 175 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 31, 197, 342, 378, 379 |
| AgsI | TTSAA | 1 cut(s) | 529 |
| AjnI | CCWGG | 4 cut(s) | 341, 374, 402, 431 |
| AluBI | AGCT | 5 cut(s) | 10, 118, 256, 389, 495 |
| AluI | AGCT | 5 cut(s) | 10, 118, 256, 389, 495 |
| Alw21I | GWGCWC | 2 cut(s) | 60, 213 |
| Alw26I | GTCTC | 1 cut(s) | 241 |
| AlwI | GGATC | 2 cut(s) | 430, 443 |
| AoxI | GGCC | 7 cut(s) | 69, 75, 90, 344, 419, 444, 452 |
| ApeKI | GCWGC | 1 cut(s) | 492 |
| ApoI | RAATTY | 1 cut(s) | 107 |
| AspLEI | GCGC | 3 cut(s) | 130, 154, 324 |
| AspS9I | GGNCC | 5 cut(s) | 22, 76, 410, 419, 444 |
| AsuHPI | GGTGA | 1 cut(s) | 418 |
| AvaII | GGWCC | 2 cut(s) | 22, 410 |
| BalI | TGGCCA | 1 cut(s) | 92 |
| BamHI | GGATCC | 1 cut(s) | 435 |
| Bbv12I | GWGCWC | 2 cut(s) | 60, 213 |
| BbvI | GCAGC | 1 cut(s) | 504 |
| BccI | CCATC | 3 cut(s) | 256, 289, 455 |
| BciT130I | CCWGG | 4 cut(s) | 343, 376, 404, 433 |
| BcoDI | GTCTC | 1 cut(s) | 241 |
| BfoI | RGCGCY | 1 cut(s) | 131 |
| BisI | GCNGC | 1 cut(s) | 493 |
| BlpI | GCTNAGC | 1 cut(s) | 390 |
| BlsI | GCNGC | 1 cut(s) | 494 |
| Bme1390I | CCNGG | 4 cut(s) | 343, 376, 404, 433 |
| Bme18I | GGWCC | 2 cut(s) | 22, 410 |
| BmgT120I | GGNCC | 5 cut(s) | 22, 76, 410, 419, 444 |
| BmiI | GGNNCC | 3 cut(s) | 398, 412, 437 |
| BmrFI | CCNGG | 4 cut(s) | 343, 376, 404, 433 |
| BmrI | ACTGGG | 1 cut(s) | 426 |
| BmsI | GCATC | 1 cut(s) | 468 |
| BmuI | ACTGGG | 1 cut(s) | 426 |
| BpmI | CTGGAG | 1 cut(s) | 386 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 390 |
| BsaHI | GRCGYC | 1 cut(s) | 561 |
| BsaJI | CCNNGG | 2 cut(s) | 72, 374 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 31, 197, 342, 378, 379 |
| Bse1I | ACTGG | 2 cut(s) | 218, 421 |
| BseBI | CCWGG | 4 cut(s) | 343, 376, 404, 433 |
| BseDI | CCNNGG | 2 cut(s) | 72, 374 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 31, 197, 342, 378, 379 |
| BseMII | CTCAG | 1 cut(s) | 404 |
| BseNI | ACTGG | 2 cut(s) | 218, 421 |
| BseXI | GCAGC | 1 cut(s) | 504 |
| BseYI | CCCAGC | 1 cut(s) | 148 |
| BshFI | GGCC | 7 cut(s) | 71, 77, 92, 346, 421, 446, 454 |
| BsiHKAI | GWGCWC | 2 cut(s) | 60, 213 |
| BslFI | GGGAC | 1 cut(s) | 255 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 31, 197, 342, 378, 379 |
| BsmAI | GTCTC | 1 cut(s) | 241 |
| BsmFI | GGGAC | 1 cut(s) | 255 |
| BsnI | GGCC | 7 cut(s) | 71, 77, 92, 346, 421, 446, 454 |
| Bsp1286I | GDGCHC | 2 cut(s) | 60, 213 |
| Bsp143I | GATC | 1 cut(s) | 435 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 390 |
| BspACI | CCGC | 6 cut(s) | 20, 308, 331, 365, 394, 442 |
| BspANI | GGCC | 7 cut(s) | 71, 77, 92, 346, 421, 446, 454 |
| BspCNI | CTCAG | 1 cut(s) | 403 |
| BspLI | GGNNCC | 3 cut(s) | 398, 412, 437 |
| BspPI | GGATC | 2 cut(s) | 430, 443 |
| BsrBI | CCGCTC | 1 cut(s) | 333 |
| BsrI | ACTGG | 2 cut(s) | 218, 421 |
| BssECI | CCNNGG | 2 cut(s) | 72, 374 |
| BssMI | GATC | 1 cut(s) | 435 |
| BssNI | GRCGYC | 1 cut(s) | 561 |
| Bst2UI | CCWGG | 4 cut(s) | 343, 376, 404, 433 |
| BstACI | GRCGYC | 1 cut(s) | 561 |
| BstC8I | GCNNGC | 3 cut(s) | 369, 444, 456 |
| BstDEI | CTNAG | 1 cut(s) | 390 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 29 |
| BstH2I | RGCGCY | 1 cut(s) | 131 |
| BstHHI | GCGC | 3 cut(s) | 130, 154, 324 |
| BstKTI | GATC | 1 cut(s) | 438 |
| BstMAI | GTCTC | 1 cut(s) | 241 |
| BstMBI | GATC | 1 cut(s) | 435 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 134, 149, 238, 330, 478 |
| BstNI | CCWGG | 4 cut(s) | 343, 376, 404, 433 |
| BstNSI | RCATGY | 1 cut(s) | 464 |
| BstSCI | CCNGG | 4 cut(s) | 341, 374, 402, 431 |
| BstV1I | GCAGC | 1 cut(s) | 504 |
| BstX2I | RGATCY | 1 cut(s) | 435 |
| BstYI | RGATCY | 1 cut(s) | 435 |
| BsuRI | GGCC | 7 cut(s) | 71, 77, 92, 346, 421, 446, 454 |
| BtsIMutI | CAGTG | 1 cut(s) | 414 |
| Cac8I | GCNNGC | 3 cut(s) | 369, 444, 456 |
| CfoI | GCGC | 3 cut(s) | 130, 154, 324 |
| Cfr13I | GGNCC | 5 cut(s) | 22, 76, 410, 419, 444 |
| Csp6I | GTAC | 1 cut(s) | 174 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 270, 305 |
| CviAII | CATG | 2 cut(s) | 100, 461 |
| CviQI | GTAC | 1 cut(s) | 174 |
| DdeI | CTNAG | 1 cut(s) | 390 |
| DpnI | GATC | 1 cut(s) | 437 |
| DpnII | GATC | 1 cut(s) | 435 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 509 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 509 |
| EaeI | YGGCCR | 1 cut(s) | 90 |
| EciI | GGCGGA | 2 cut(s) | 35, 354 |
| Eco47I | GGWCC | 2 cut(s) | 22, 410 |
| Eco57I | CTGAAG | 1 cut(s) | 140 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 29 |
| EcoO109I | RGGNCCY | 1 cut(s) | 410 |
| EcoRII | CCWGG | 4 cut(s) | 341, 374, 402, 431 |
| FaeI | CATG | 2 cut(s) | 103, 464 |
| FaiI | YATR | 9 cut(s) | 6, 101, 171, 228, 230, 462, 489, 516, 518 |
| FaqI | GGGAC | 1 cut(s) | 255 |
| FatI | CATG | 2 cut(s) | 99, 460 |
| FauI | CCCGC | 1 cut(s) | 435 |
| FauNDI | CATATG | 2 cut(s) | 228, 516 |
| FblI | GTMKAC | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 1 cut(s) | 493 |
| Fsp4HI | GCNGC | 1 cut(s) | 493 |
| FspI | TGCGCA | 1 cut(s) | 323 |
| GlaI | GCGC | 3 cut(s) | 129, 153, 323 |
| GluI | GCNGC | 1 cut(s) | 493 |
| GsaI | CCCAGC | 1 cut(s) | 152 |
| GsuI | CTGGAG | 1 cut(s) | 386 |
| HaeII | RGCGCY | 1 cut(s) | 131 |
| HaeIII | GGCC | 7 cut(s) | 71, 77, 92, 346, 421, 446, 454 |
| HhaI | GCGC | 3 cut(s) | 130, 154, 324 |
| Hin1I | GRCGYC | 1 cut(s) | 561 |
| Hin1II | CATG | 2 cut(s) | 103, 464 |
| Hin6I | GCGC | 3 cut(s) | 128, 152, 322 |
| HinP1I | GCGC | 3 cut(s) | 128, 152, 322 |
| HincII | GTYRAC | 1 cut(s) | 188 |
| HindII | GTYRAC | 1 cut(s) | 188 |
| HinfI | GANTC | 4 cut(s) | 268, 311, 351, 552 |
| HphI | GGTGA | 1 cut(s) | 418 |
| Hpy166II | GTNNAC | 2 cut(s) | 50, 188 |
| Hpy188I | TCNGA | 1 cut(s) | 253 |
| Hpy188III | TCNNGA | 1 cut(s) | 529 |
| Hpy8I | GTNNAC | 2 cut(s) | 50, 188 |
| Hpy99I | CGWCG | 2 cut(s) | 116, 200 |
| HpyAV | CCTTC | 3 cut(s) | 118, 517, 535 |
| HpyCH4IV | ACGT | 1 cut(s) | 181 |
| HpyCH4V | TGCA | 2 cut(s) | 232, 464 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 134, 149, 238, 330, 478 |
| HpyF3I | CTNAG | 1 cut(s) | 390 |
| HpySE526I | ACGT | 1 cut(s) | 181 |
| Hsp92I | GRCGYC | 1 cut(s) | 561 |
| Hsp92II | CATG | 2 cut(s) | 103, 464 |
| HspAI | GCGC | 3 cut(s) | 128, 152, 322 |
| Kzo9I | GATC | 1 cut(s) | 435 |
| LmnI | GCTCC | 2 cut(s) | 253, 338 |
| Lsp1109I | GCAGC | 1 cut(s) | 504 |
| LweI | GCATC | 1 cut(s) | 468 |
| MaeII | ACGT | 1 cut(s) | 181 |
| MaeIII | GTNAC | 1 cut(s) | 535 |
| MalI | GATC | 1 cut(s) | 437 |
| MbiI | CCGCTC | 1 cut(s) | 333 |
| MboI | GATC | 1 cut(s) | 435 |
| MboII | GAAGA | 1 cut(s) | 133 |
| MflI | RGATCY | 1 cut(s) | 435 |
| MhlI | GDGCHC | 2 cut(s) | 60, 213 |
| MlsI | TGGCCA | 1 cut(s) | 92 |
| MluCI | AATT | 2 cut(s) | 14, 107 |
| MluNI | TGGCCA | 1 cut(s) | 92 |
| MlyI | GAGTC | 1 cut(s) | 277 |
| MnlI | CCTC | 6 cut(s) | 35, 67, 288, 349, 372, 410 |
| Mox20I | TGGCCA | 1 cut(s) | 92 |
| MscI | TGGCCA | 1 cut(s) | 92 |
| MslI | CAYNNNNRTG | 1 cut(s) | 98 |
| Msp20I | TGGCCA | 1 cut(s) | 92 |
| MspA1I | CMGCKG | 1 cut(s) | 394 |
| MspR9I | CCNGG | 4 cut(s) | 343, 376, 404, 433 |
| MvaI | CCWGG | 4 cut(s) | 343, 376, 404, 433 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 134, 149, 238, 330, 478 |
| NdeI | CATATG | 2 cut(s) | 228, 516 |
| NdeII | GATC | 1 cut(s) | 435 |
| NlaIII | CATG | 2 cut(s) | 103, 464 |
| NlaIV | GGNNCC | 3 cut(s) | 398, 412, 437 |
| NmeAIII | GCCGAG | 1 cut(s) | 97 |
| NmuCI | GTSAC | 1 cut(s) | 535 |
| NsbI | TGCGCA | 1 cut(s) | 323 |
| NspI | RCATGY | 1 cut(s) | 464 |
| PfeI | GAWTC | 3 cut(s) | 311, 351, 552 |
| PflFI | GACNNNGTC | 1 cut(s) | 183 |
| PkrI | GCNGC | 1 cut(s) | 494 |
| PleI | GAGTC | 1 cut(s) | 276 |
| PpsI | GAGTC | 1 cut(s) | 276 |
| PpuMI | RGGWCCY | 1 cut(s) | 410 |
| Psp5II | RGGWCCY | 1 cut(s) | 410 |
| Psp6I | CCWGG | 4 cut(s) | 341, 374, 402, 431 |
| PspFI | CCCAGC | 1 cut(s) | 148 |
| PspGI | CCWGG | 4 cut(s) | 341, 374, 402, 431 |
| PspN4I | GGNNCC | 3 cut(s) | 398, 412, 437 |
| PspPI | GGNCC | 5 cut(s) | 22, 76, 410, 419, 444 |
| PspPPI | RGGWCCY | 1 cut(s) | 410 |
| PsuI | RGATCY | 1 cut(s) | 435 |
| PsyI | GACNNNGTC | 1 cut(s) | 183 |
| RsaI | GTAC | 1 cut(s) | 175 |
| RsaNI | GTAC | 1 cut(s) | 174 |
| RseI | CAYNNNNRTG | 1 cut(s) | 98 |
| SalI | GTCGAC | 1 cut(s) | 186 |
| SatI | GCNGC | 1 cut(s) | 493 |
| Sau3AI | GATC | 1 cut(s) | 435 |
| Sau96I | GGNCC | 5 cut(s) | 22, 76, 410, 419, 444 |
| SchI | GAGTC | 1 cut(s) | 277 |
| ScrFI | CCNGG | 4 cut(s) | 343, 376, 404, 433 |
| SduI | GDGCHC | 2 cut(s) | 60, 213 |
| SfaNI | GCATC | 1 cut(s) | 468 |
| SinI | GGWCC | 2 cut(s) | 22, 410 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 98 |
| Sse9I | AATT | 2 cut(s) | 14, 107 |
| SsiI | CCGC | 6 cut(s) | 20, 308, 331, 365, 394, 442 |
| StyD4I | CCNGG | 4 cut(s) | 341, 374, 402, 431 |
| TaiI | ACGT | 1 cut(s) | 184 |
| TaqI | TCGA | 4 cut(s) | 111, 187, 354, 555 |
| TasI | AATT | 2 cut(s) | 14, 107 |
| TfiI | GAWTC | 3 cut(s) | 311, 351, 552 |
| TscAI | CASTG | 1 cut(s) | 421 |
| TseFI | GTSAC | 1 cut(s) | 535 |
| TseI | GCWGC | 1 cut(s) | 492 |
| Tsp45I | GTSAC | 1 cut(s) | 535 |
| TspGWI | ACGGA | 1 cut(s) | 213 |
| TspRI | CASTG | 1 cut(s) | 421 |
| Tth111I | GACNNNGTC | 1 cut(s) | 183 |
| VpaK11BI | GGWCC | 2 cut(s) | 22, 410 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 29 |
| XapI | RAATTY | 1 cut(s) | 107 |
| XceI | RCATGY | 1 cut(s) | 464 |
| XmiI | GTMKAC | 1 cut(s) | 187 |