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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 379 |
| AccII | CGCG | 1 cut(s) | 90 |
| AclWI | GGATC | 2 cut(s) | 168, 201 |
| AcoI | YGGCCR | 2 cut(s) | 108, 306 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 3 cut(s) | 176, 396, 408 |
| AfeI | AGCGCT | 1 cut(s) | 580 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 368 |
| AflIII | ACRYGT | 1 cut(s) | 275 |
| AjnI | CCWGG | 3 cut(s) | 156, 347, 382 |
| AleI | CACNNNNGTG | 1 cut(s) | 377 |
| Alw26I | GTCTC | 1 cut(s) | 540 |
| AlwI | GGATC | 2 cut(s) | 168, 201 |
| Aor51HI | AGCGCT | 1 cut(s) | 580 |
| AoxI | GGCC | 7 cut(s) | 108, 154, 240, 306, 386, 597, 683 |
| ApeKI | GCWGC | 3 cut(s) | 138, 141, 263 |
| AspLEI | GCGC | 5 cut(s) | 92, 231, 368, 581, 631 |
| AspS9I | GGNCC | 5 cut(s) | 386, 429, 589, 597, 683 |
| AsuC2I | CCSGG | 2 cut(s) | 107, 320 |
| AsuHPI | GGTGA | 2 cut(s) | 134, 160 |
| AsuNHI | GCTAGC | 1 cut(s) | 502 |
| AvaII | GGWCC | 2 cut(s) | 429, 589 |
| BanI | GGYRCC | 1 cut(s) | 379 |
| BbvI | GCAGC | 3 cut(s) | 150, 153, 250 |
| BccI | CCATC | 3 cut(s) | 253, 464, 552 |
| BceAI | ACGGC | 2 cut(s) | 515, 517 |
| BciT130I | CCWGG | 3 cut(s) | 158, 349, 384 |
| BcnI | CCSGG | 2 cut(s) | 107, 320 |
| BcoDI | GTCTC | 1 cut(s) | 540 |
| BfaI | CTAG | 2 cut(s) | 134, 503 |
| BfoI | RGCGCY | 2 cut(s) | 369, 582 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 435, 676 |
| BlsI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 307, 436, 677 |
| Bme1390I | CCNGG | 5 cut(s) | 107, 158, 320, 349, 384 |
| Bme18I | GGWCC | 2 cut(s) | 429, 589 |
| BmgT120I | GGNCC | 5 cut(s) | 386, 429, 589, 597, 683 |
| BmiI | GGNNCC | 2 cut(s) | 381, 430 |
| BmrFI | CCNGG | 5 cut(s) | 107, 158, 320, 349, 384 |
| BmrI | ACTGGG | 1 cut(s) | 502 |
| BmsI | GCATC | 2 cut(s) | 553, 653 |
| BmtI | GCTAGC | 1 cut(s) | 506 |
| BmuI | ACTGGG | 1 cut(s) | 502 |
| BpuMI | CCSGG | 2 cut(s) | 107, 320 |
| BsaAI | YACGTR | 1 cut(s) | 341 |
| BsaJI | CCNNGG | 3 cut(s) | 105, 383, 467 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 368 |
| Bse118I | RCCGGY | 2 cut(s) | 368, 388 |
| Bse1I | ACTGG | 2 cut(s) | 192, 508 |
| BseBI | CCWGG | 3 cut(s) | 158, 349, 384 |
| BseDI | CCNNGG | 3 cut(s) | 105, 383, 467 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 368 |
| BseNI | ACTGG | 2 cut(s) | 192, 508 |
| BseRI | GAGGAG | 5 cut(s) | 577, 580, 583, 586, 589 |
| BseXI | GCAGC | 3 cut(s) | 150, 153, 250 |
| BseYI | CCCAGC | 2 cut(s) | 116, 362 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| Bsh1285I | CGRYCG | 1 cut(s) | 393 |
| BshFI | GGCC | 7 cut(s) | 110, 156, 242, 308, 388, 599, 685 |
| BshNI | GGYRCC | 1 cut(s) | 379 |
| BsiEI | CGRYCG | 1 cut(s) | 393 |
| BsiSI | CCGG | 4 cut(s) | 107, 320, 369, 389 |
| BslFI | GGGAC | 1 cut(s) | 442 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 368 |
| BsmAI | GTCTC | 1 cut(s) | 540 |
| BsmBI | CGTCTC | 1 cut(s) | 540 |
| BsmFI | GGGAC | 1 cut(s) | 442 |
| BsnI | GGCC | 7 cut(s) | 110, 156, 242, 308, 388, 599, 685 |
| Bsp143I | GATC | 3 cut(s) | 160, 193, 636 |
| Bsp19I | CCATGG | 1 cut(s) | 467 |
| BspANI | GGCC | 7 cut(s) | 110, 156, 242, 308, 388, 599, 685 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 2 cut(s) | 381, 430 |
| BspOI | GCTAGC | 1 cut(s) | 506 |
| BspPI | GGATC | 2 cut(s) | 168, 201 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 379 |
| BsrFI | RCCGGY | 2 cut(s) | 368, 388 |
| BsrI | ACTGG | 2 cut(s) | 192, 508 |
| BssAI | RCCGGY | 2 cut(s) | 368, 388 |
| BssECI | CCNNGG | 3 cut(s) | 105, 383, 467 |
| BssMI | GATC | 3 cut(s) | 160, 193, 636 |
| BssT1I | CCWWGG | 1 cut(s) | 467 |
| Bst2UI | CCWGG | 3 cut(s) | 158, 349, 384 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstBAI | YACGTR | 1 cut(s) | 341 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 332, 370, 504, 663 |
| BstDSI | CCRYGG | 1 cut(s) | 467 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstH2I | RGCGCY | 2 cut(s) | 369, 582 |
| BstHHI | GCGC | 5 cut(s) | 92, 231, 368, 581, 631 |
| BstKTI | GATC | 3 cut(s) | 163, 196, 639 |
| BstMAI | GTCTC | 1 cut(s) | 540 |
| BstMBI | GATC | 3 cut(s) | 160, 193, 636 |
| BstMCI | CGRYCG | 1 cut(s) | 393 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 87, 144, 153, 409, 499 |
| BstNI | CCWGG | 3 cut(s) | 158, 349, 384 |
| BstNSI | RCATGY | 1 cut(s) | 407 |
| BstSCI | CCNGG | 5 cut(s) | 105, 156, 318, 347, 382 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 3 cut(s) | 150, 153, 250 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 7 cut(s) | 110, 156, 242, 308, 388, 599, 685 |
| BtgI | CCRYGG | 1 cut(s) | 467 |
| BtgZI | GCGATG | 1 cut(s) | 630 |
| BtsIMutI | CAGTG | 2 cut(s) | 185, 515 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 332, 370, 504, 663 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 5 cut(s) | 92, 231, 368, 581, 631 |
| Cfr10I | RCCGGY | 2 cut(s) | 368, 388 |
| Cfr13I | GGNCC | 5 cut(s) | 386, 429, 589, 597, 683 |
| Csp6I | GTAC | 3 cut(s) | 175, 395, 407 |
| CviAII | CATG | 5 cut(s) | 172, 271, 404, 468, 514 |
| CviQI | GTAC | 3 cut(s) | 175, 395, 407 |
| DpnI | GATC | 3 cut(s) | 162, 195, 638 |
| DpnII | GATC | 3 cut(s) | 160, 193, 636 |
| EaeI | YGGCCR | 2 cut(s) | 108, 306 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 3 cut(s) | 206, 239, 602 |
| Eco130I | CCWWGG | 1 cut(s) | 467 |
| Eco47I | GGWCC | 2 cut(s) | 429, 589 |
| Eco47III | AGCGCT | 1 cut(s) | 580 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoO109I | RGGNCCY | 1 cut(s) | 683 |
| EcoRII | CCWGG | 3 cut(s) | 156, 347, 382 |
| EcoT14I | CCWWGG | 1 cut(s) | 467 |
| EcoT22I | ATGCAT | 1 cut(s) | 405 |
| ErhI | CCWWGG | 1 cut(s) | 467 |
| Esp3I | CGTCTC | 1 cut(s) | 540 |
| FaeI | CATG | 5 cut(s) | 175, 274, 407, 471, 517 |
| FaiI | YATR | 7 cut(s) | 173, 201, 272, 405, 441, 469, 515 |
| FaqI | GGGAC | 1 cut(s) | 442 |
| FatI | CATG | 5 cut(s) | 171, 270, 403, 467, 513 |
| FauI | CCCGC | 3 cut(s) | 8, 55, 79 |
| Fnu4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 435, 676 |
| Fsp4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 435, 676 |
| FspBI | CTAG | 2 cut(s) | 134, 503 |
| GlaI | GCGC | 5 cut(s) | 91, 230, 367, 580, 630 |
| GluI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 435, 676 |
| GsaI | CCCAGC | 2 cut(s) | 120, 366 |
| HaeII | RGCGCY | 2 cut(s) | 369, 582 |
| HaeIII | GGCC | 7 cut(s) | 110, 156, 242, 308, 388, 599, 685 |
| HapII | CCGG | 4 cut(s) | 107, 320, 369, 389 |
| HhaI | GCGC | 5 cut(s) | 92, 231, 368, 581, 631 |
| Hin1II | CATG | 5 cut(s) | 175, 274, 407, 471, 517 |
| Hin6I | GCGC | 5 cut(s) | 90, 229, 366, 579, 629 |
| HinP1I | GCGC | 5 cut(s) | 90, 229, 366, 579, 629 |
| HincII | GTYRAC | 2 cut(s) | 85, 359 |
| HindII | GTYRAC | 2 cut(s) | 85, 359 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 4 cut(s) | 107, 320, 369, 389 |
| HphI | GGTGA | 2 cut(s) | 134, 160 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 359 |
| Hpy188I | TCNGA | 3 cut(s) | 67, 626, 641 |
| Hpy188III | TCNNGA | 3 cut(s) | 271, 421, 551 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 359 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyCH4IV | ACGT | 2 cut(s) | 277, 340 |
| HpyCH4V | TGCA | 3 cut(s) | 403, 493, 513 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 87, 144, 153, 409, 499 |
| HpySE526I | ACGT | 2 cut(s) | 277, 340 |
| Hsp92II | CATG | 5 cut(s) | 175, 274, 407, 471, 517 |
| HspAI | GCGC | 5 cut(s) | 90, 229, 366, 579, 629 |
| KroI | GCCGGC | 1 cut(s) | 368 |
| KroNI | GCCGGC | 1 cut(s) | 370 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 3 cut(s) | 160, 193, 636 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 4 cut(s) | 226, 576, 626, 641 |
| Lsp1109I | GCAGC | 3 cut(s) | 150, 153, 250 |
| LweI | GCATC | 2 cut(s) | 553, 653 |
| MaeI | CTAG | 2 cut(s) | 134, 503 |
| MaeII | ACGT | 2 cut(s) | 277, 340 |
| MaeIII | GTNAC | 3 cut(s) | 166, 336, 551 |
| MalI | GATC | 3 cut(s) | 162, 195, 638 |
| MboI | GATC | 3 cut(s) | 160, 193, 636 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| Mph1103I | ATGCAT | 1 cut(s) | 405 |
| MroNI | GCCGGC | 1 cut(s) | 368 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 2 cut(s) | 377, 549 |
| MspA1I | CMGCKG | 2 cut(s) | 120, 412 |
| MspI | CCGG | 4 cut(s) | 107, 320, 369, 389 |
| MspR9I | CCNGG | 5 cut(s) | 107, 158, 320, 349, 384 |
| MvaI | CCWGG | 3 cut(s) | 158, 349, 384 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 87, 144, 153, 409, 499 |
| NaeI | GCCGGC | 1 cut(s) | 370 |
| NciI | CCSGG | 2 cut(s) | 107, 320 |
| NcoI | CCATGG | 1 cut(s) | 467 |
| NdeII | GATC | 3 cut(s) | 160, 193, 636 |
| NgoMIV | GCCGGC | 1 cut(s) | 368 |
| NheI | GCTAGC | 1 cut(s) | 502 |
| NlaIII | CATG | 5 cut(s) | 175, 274, 407, 471, 517 |
| NlaIV | GGNNCC | 2 cut(s) | 381, 430 |
| NmeAIII | GCCGAG | 1 cut(s) | 625 |
| NmuCI | GTSAC | 3 cut(s) | 166, 336, 551 |
| NsiI | ATGCAT | 1 cut(s) | 405 |
| NspI | RCATGY | 1 cut(s) | 407 |
| OliI | CACNNNNGTG | 1 cut(s) | 377 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PdiI | GCCGGC | 1 cut(s) | 370 |
| PkrI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 307, 436, 677 |
| Ppu21I | YACGTR | 1 cut(s) | 341 |
| Psp6I | CCWGG | 3 cut(s) | 156, 347, 382 |
| PspFI | CCCAGC | 2 cut(s) | 116, 362 |
| PspGI | CCWGG | 3 cut(s) | 156, 347, 382 |
| PspN4I | GGNNCC | 2 cut(s) | 381, 430 |
| PspPI | GGNCC | 5 cut(s) | 386, 429, 589, 597, 683 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| RsaI | GTAC | 3 cut(s) | 176, 396, 408 |
| RsaNI | GTAC | 3 cut(s) | 175, 395, 407 |
| RseI | CAYNNNNRTG | 2 cut(s) | 377, 549 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 435, 676 |
| Sau3AI | GATC | 3 cut(s) | 160, 193, 636 |
| Sau96I | GGNCC | 5 cut(s) | 386, 429, 589, 597, 683 |
| ScrFI | CCNGG | 5 cut(s) | 107, 158, 320, 349, 384 |
| SetI | ASST | 3 cut(s) | 280, 343, 446 |
| SfaNI | GCATC | 2 cut(s) | 553, 653 |
| SinI | GGWCC | 2 cut(s) | 429, 589 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 377, 549 |
| SspMI | CTAG | 2 cut(s) | 134, 503 |
| StyD4I | CCNGG | 5 cut(s) | 105, 156, 318, 347, 382 |
| StyI | CCWWGG | 1 cut(s) | 467 |
| TaiI | ACGT | 2 cut(s) | 280, 343 |
| TauI | GCSGC | 4 cut(s) | 147, 308, 437, 678 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 2 cut(s) | 192, 515 |
| TseFI | GTSAC | 3 cut(s) | 166, 336, 551 |
| TseI | GCWGC | 3 cut(s) | 138, 141, 263 |
| Tsp45I | GTSAC | 3 cut(s) | 166, 336, 551 |
| TspDTI | ATGAA | 2 cut(s) | 188, 530 |
| TspGWI | ACGGA | 1 cut(s) | 669 |
| TspRI | CASTG | 2 cut(s) | 192, 515 |
| VpaK11BI | GGWCC | 2 cut(s) | 429, 589 |
| XceI | RCATGY | 1 cut(s) | 407 |
| XspI | CTAG | 2 cut(s) | 134, 503 |
| Zsp2I | ATGCAT | 1 cut(s) | 405 |