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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 671 |
| Acc36I | ACCTGC | 1 cut(s) | 556 |
| AccB1I | GGYRCC | 1 cut(s) | 379 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccBSI | CCGCTC | 1 cut(s) | 504 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 4 cut(s) | 74, 90, 277, 727 |
| AclWI | GGATC | 4 cut(s) | 168, 201, 527, 723 |
| AcoI | YGGCCR | 3 cut(s) | 108, 261, 306 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AcyI | GRCGYC | 1 cut(s) | 668 |
| AfaI | GTAC | 3 cut(s) | 176, 396, 408 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 784 |
| AflIII | ACRYGT | 1 cut(s) | 275 |
| AjnI | CCWGG | 5 cut(s) | 133, 156, 347, 382, 421 |
| AleI | CACNNNNGTG | 1 cut(s) | 377 |
| AluBI | AGCT | 1 cut(s) | 607 |
| AluI | AGCT | 1 cut(s) | 607 |
| Alw26I | GTCTC | 1 cut(s) | 647 |
| AlwI | GGATC | 4 cut(s) | 168, 201, 527, 723 |
| Ama87I | CYCGRG | 1 cut(s) | 513 |
| ApeKI | GCWGC | 3 cut(s) | 138, 141, 342 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 486 |
| AspA2I | CCTAGG | 1 cut(s) | 545 |
| AspLEI | GCGC | 4 cut(s) | 92, 231, 368, 552 |
| AspS9I | GGNCC | 3 cut(s) | 386, 440, 704 |
| AsuC2I | CCSGG | 6 cut(s) | 107, 117, 320, 514, 515, 702 |
| AsuHPI | GGTGA | 2 cut(s) | 134, 160 |
| AvaI | CYCGRG | 1 cut(s) | 513 |
| AvaII | GGWCC | 1 cut(s) | 440 |
| AvrII | CCTAGG | 1 cut(s) | 545 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| BanI | GGYRCC | 1 cut(s) | 379 |
| BbvI | GCAGC | 3 cut(s) | 150, 153, 354 |
| BccI | CCATC | 2 cut(s) | 59, 253 |
| BceAI | ACGGC | 2 cut(s) | 544, 724 |
| BciT130I | CCWGG | 5 cut(s) | 135, 158, 349, 384, 423 |
| BcnI | CCSGG | 6 cut(s) | 107, 117, 320, 514, 515, 702 |
| BcoDI | GTCTC | 1 cut(s) | 647 |
| BfaI | CTAG | 1 cut(s) | 546 |
| BfmI | CTRYAG | 1 cut(s) | 733 |
| BfoI | RGCGCY | 1 cut(s) | 369 |
| BfuAI | ACCTGC | 1 cut(s) | 556 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 562 |
| BisI | GCNGC | 8 cut(s) | 139, 142, 145, 306, 343, 446, 588, 725 |
| BlnI | CCTAGG | 1 cut(s) | 545 |
| BlsI | GCNGC | 8 cut(s) | 140, 143, 146, 307, 344, 447, 589, 726 |
| Bme18I | GGWCC | 1 cut(s) | 440 |
| BmeT110I | CYCGRG | 1 cut(s) | 513 |
| BmgT120I | GGNCC | 3 cut(s) | 386, 440, 704 |
| BmiI | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| BmsI | GCATC | 2 cut(s) | 583, 609 |
| BoxI | GACNNNNGTC | 1 cut(s) | 659 |
| BpmI | CTGGAG | 1 cut(s) | 650 |
| BpuMI | CCSGG | 6 cut(s) | 107, 117, 320, 514, 515, 702 |
| BsaHI | GRCGYC | 1 cut(s) | 668 |
| BsaJI | CCNNGG | 6 cut(s) | 105, 115, 383, 422, 513, 545 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 623, 653 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 784 |
| Bse118I | RCCGGY | 2 cut(s) | 368, 388 |
| Bse1I | ACTGG | 2 cut(s) | 192, 633 |
| BseBI | CCWGG | 5 cut(s) | 135, 158, 349, 384, 423 |
| BseDI | CCNNGG | 6 cut(s) | 105, 115, 383, 422, 513, 545 |
| BseGI | GGATG | 2 cut(s) | 51, 624 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 784 |
| BseNI | ACTGG | 2 cut(s) | 192, 633 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseXI | GCAGC | 3 cut(s) | 150, 153, 354 |
| BseYI | CCCAGC | 2 cut(s) | 362, 603 |
| Bsh1236I | CGCG | 4 cut(s) | 74, 90, 277, 727 |
| Bsh1285I | CGRYCG | 3 cut(s) | 78, 316, 393 |
| BshNI | GGYRCC | 1 cut(s) | 379 |
| BsiEI | CGRYCG | 3 cut(s) | 78, 316, 393 |
| BsiHKCI | CYCGRG | 1 cut(s) | 513 |
| BsiSI | CCGG | 7 cut(s) | 107, 117, 320, 369, 389, 514, 702 |
| BslFI | GGGAC | 2 cut(s) | 453, 647 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 784 |
| BsmAI | GTCTC | 1 cut(s) | 647 |
| BsmBI | CGTCTC | 1 cut(s) | 647 |
| BsmFI | GGGAC | 2 cut(s) | 453, 647 |
| BsmI | GAATGC | 1 cut(s) | 514 |
| BsoBI | CYCGRG | 1 cut(s) | 513 |
| Bsp143I | GATC | 6 cut(s) | 75, 160, 193, 532, 642, 728 |
| BspFNI | CGCG | 4 cut(s) | 74, 90, 277, 727 |
| BspLI | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| BspMI | ACCTGC | 1 cut(s) | 556 |
| BspPI | GGATC | 4 cut(s) | 168, 201, 527, 723 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 379 |
| BsrBI | CCGCTC | 1 cut(s) | 504 |
| BsrFI | RCCGGY | 2 cut(s) | 368, 388 |
| BsrI | ACTGG | 2 cut(s) | 192, 633 |
| BssAI | RCCGGY | 2 cut(s) | 368, 388 |
| BssECI | CCNNGG | 6 cut(s) | 105, 115, 383, 422, 513, 545 |
| BssMI | GATC | 6 cut(s) | 75, 160, 193, 532, 642, 728 |
| BssNI | GRCGYC | 1 cut(s) | 668 |
| BssT1I | CCWWGG | 1 cut(s) | 545 |
| Bst2UI | CCWGG | 5 cut(s) | 135, 158, 349, 384, 423 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstACI | GRCGYC | 1 cut(s) | 668 |
| BstC8I | GCNNGC | 3 cut(s) | 332, 370, 554 |
| BstF5I | GGATG | 2 cut(s) | 51, 624 |
| BstFNI | CGCG | 4 cut(s) | 74, 90, 277, 727 |
| BstH2I | RGCGCY | 1 cut(s) | 369 |
| BstHHI | GCGC | 4 cut(s) | 92, 231, 368, 552 |
| BstKTI | GATC | 6 cut(s) | 78, 163, 196, 535, 645, 731 |
| BstMAI | GTCTC | 1 cut(s) | 647 |
| BstMBI | GATC | 6 cut(s) | 75, 160, 193, 532, 642, 728 |
| BstMCI | CGRYCG | 3 cut(s) | 78, 316, 393 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 87, 144, 153, 409, 562, 584, 593 |
| BstNI | CCWGG | 5 cut(s) | 135, 158, 349, 384, 423 |
| BstNSI | RCATGY | 1 cut(s) | 407 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 659 |
| BstSFI | CTRYAG | 1 cut(s) | 733 |
| BstUI | CGCG | 4 cut(s) | 74, 90, 277, 727 |
| BstV1I | GCAGC | 3 cut(s) | 150, 153, 354 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BtsCI | GGATG | 2 cut(s) | 51, 624 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| BveI | ACCTGC | 1 cut(s) | 556 |
| Cac8I | GCNNGC | 3 cut(s) | 332, 370, 554 |
| CfoI | GCGC | 4 cut(s) | 92, 231, 368, 552 |
| Cfr10I | RCCGGY | 2 cut(s) | 368, 388 |
| Cfr13I | GGNCC | 3 cut(s) | 386, 440, 704 |
| Cfr9I | CCCGGG | 1 cut(s) | 513 |
| CseI | GACGC | 1 cut(s) | 283 |
| Csp6I | GTAC | 3 cut(s) | 175, 395, 407 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 2 cut(s) | 172, 404 |
| CviQI | GTAC | 3 cut(s) | 175, 395, 407 |
| DpnI | GATC | 6 cut(s) | 77, 162, 195, 534, 644, 730 |
| DpnII | GATC | 6 cut(s) | 75, 160, 193, 532, 642, 728 |
| EaeI | YGGCCR | 3 cut(s) | 108, 261, 306 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 7 cut(s) | 206, 494, 525, 574, 665, 703, 711 |
| Eco130I | CCWWGG | 1 cut(s) | 545 |
| Eco47I | GGWCC | 1 cut(s) | 440 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| Eco88I | CYCGRG | 1 cut(s) | 513 |
| EcoRII | CCWGG | 5 cut(s) | 133, 156, 347, 382, 421 |
| EcoT14I | CCWWGG | 1 cut(s) | 545 |
| EcoT22I | ATGCAT | 1 cut(s) | 405 |
| ErhI | CCWWGG | 1 cut(s) | 545 |
| Esp3I | CGTCTC | 1 cut(s) | 647 |
| FaeI | CATG | 2 cut(s) | 175, 407 |
| FaqI | GGGAC | 2 cut(s) | 453, 647 |
| FatI | CATG | 2 cut(s) | 171, 403 |
| FauI | CCCGC | 3 cut(s) | 8, 55, 79 |
| FauNDI | CATATG | 2 cut(s) | 753, 780 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 8 cut(s) | 139, 142, 145, 306, 343, 446, 588, 725 |
| FokI | GGATG | 2 cut(s) | 38, 631 |
| Fsp4HI | GCNGC | 8 cut(s) | 139, 142, 145, 306, 343, 446, 588, 725 |
| FspBI | CTAG | 1 cut(s) | 546 |
| GlaI | GCGC | 4 cut(s) | 91, 230, 367, 551 |
| GluI | GCNGC | 8 cut(s) | 139, 142, 145, 306, 343, 446, 588, 725 |
| GsaI | CCCAGC | 2 cut(s) | 366, 607 |
| GsuI | CTGGAG | 1 cut(s) | 650 |
| HaeII | RGCGCY | 1 cut(s) | 369 |
| HapII | CCGG | 7 cut(s) | 107, 117, 320, 369, 389, 514, 702 |
| HgaI | GACGC | 1 cut(s) | 283 |
| HhaI | GCGC | 4 cut(s) | 92, 231, 368, 552 |
| Hin1I | GRCGYC | 1 cut(s) | 668 |
| Hin1II | CATG | 2 cut(s) | 175, 407 |
| Hin6I | GCGC | 4 cut(s) | 90, 229, 366, 550 |
| HinP1I | GCGC | 4 cut(s) | 90, 229, 366, 550 |
| HincII | GTYRAC | 3 cut(s) | 85, 359, 672 |
| HindII | GTYRAC | 3 cut(s) | 85, 359, 672 |
| HinfI | GANTC | 2 cut(s) | 482, 581 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 7 cut(s) | 107, 117, 320, 369, 389, 514, 702 |
| HphI | GGTGA | 2 cut(s) | 134, 160 |
| Hpy166II | GTNNAC | 3 cut(s) | 85, 359, 672 |
| Hpy188I | TCNGA | 2 cut(s) | 67, 678 |
| Hpy188III | TCNNGA | 3 cut(s) | 432, 664, 696 |
| Hpy8I | GTNNAC | 3 cut(s) | 85, 359, 672 |
| Hpy99I | CGWCG | 1 cut(s) | 670 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 2 cut(s) | 217, 668 |
| HpyCH4V | TGCA | 4 cut(s) | 403, 596, 622, 757 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 87, 144, 153, 409, 562, 584, 593 |
| HpySE526I | ACGT | 2 cut(s) | 217, 668 |
| Hsp92I | GRCGYC | 1 cut(s) | 668 |
| Hsp92II | CATG | 2 cut(s) | 175, 407 |
| HspAI | GCGC | 4 cut(s) | 90, 229, 366, 550 |
| KroI | GCCGGC | 1 cut(s) | 368 |
| KroNI | GCCGGC | 1 cut(s) | 370 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 6 cut(s) | 75, 160, 193, 532, 642, 728 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 2 cut(s) | 226, 631 |
| Lsp1109I | GCAGC | 3 cut(s) | 150, 153, 354 |
| LweI | GCATC | 2 cut(s) | 583, 609 |
| MaeI | CTAG | 1 cut(s) | 546 |
| MaeII | ACGT | 2 cut(s) | 217, 668 |
| MaeIII | GTNAC | 2 cut(s) | 166, 336 |
| MalI | GATC | 6 cut(s) | 77, 162, 195, 534, 644, 730 |
| MbiI | CCGCTC | 1 cut(s) | 504 |
| MboI | GATC | 6 cut(s) | 75, 160, 193, 532, 642, 728 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluI | ACGCGT | 1 cut(s) | 275 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MmeI | TCCRAC | 2 cut(s) | 82, 90 |
| MnlI | CCTC | 7 cut(s) | 65, 73, 118, 214, 217, 277, 630 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| Mph1103I | ATGCAT | 1 cut(s) | 405 |
| MroNI | GCCGGC | 1 cut(s) | 368 |
| MroXI | GAANNNNTTC | 1 cut(s) | 486 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 2 cut(s) | 269, 377 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 1 cut(s) | 412 |
| MspI | CCGG | 7 cut(s) | 107, 117, 320, 369, 389, 514, 702 |
| Mva1269I | GAATGC | 1 cut(s) | 514 |
| MvaI | CCWGG | 5 cut(s) | 135, 158, 349, 384, 423 |
| MvnI | CGCG | 4 cut(s) | 74, 90, 277, 727 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 87, 144, 153, 409, 562, 584, 593 |
| NaeI | GCCGGC | 1 cut(s) | 370 |
| NciI | CCSGG | 6 cut(s) | 107, 117, 320, 514, 515, 702 |
| NdeI | CATATG | 2 cut(s) | 753, 780 |
| NdeII | GATC | 6 cut(s) | 75, 160, 193, 532, 642, 728 |
| NgoMIV | GCCGGC | 1 cut(s) | 368 |
| NlaIII | CATG | 2 cut(s) | 175, 407 |
| NlaIV | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| NmeAIII | GCCGAG | 1 cut(s) | 541 |
| NmuCI | GTSAC | 2 cut(s) | 166, 336 |
| NsiI | ATGCAT | 1 cut(s) | 405 |
| NspI | RCATGY | 1 cut(s) | 407 |
| OliI | CACNNNNGTG | 1 cut(s) | 377 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 662 |
| PctI | GAATGC | 1 cut(s) | 514 |
| PdiI | GCCGGC | 1 cut(s) | 370 |
| PdmI | GAANNNNTTC | 1 cut(s) | 486 |
| PfeI | GAWTC | 2 cut(s) | 482, 581 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 8 cut(s) | 140, 143, 146, 307, 344, 447, 589, 726 |
| Ple19I | CGATCG | 1 cut(s) | 78 |
| PshAI | GACNNNNGTC | 1 cut(s) | 659 |
| Psp6I | CCWGG | 5 cut(s) | 133, 156, 347, 382, 421 |
| PspFI | CCCAGC | 2 cut(s) | 362, 603 |
| PspGI | CCWGG | 5 cut(s) | 133, 156, 347, 382, 421 |
| PspN4I | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| PspPI | GGNCC | 3 cut(s) | 386, 440, 704 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PvuI | CGATCG | 1 cut(s) | 78 |
| RsaI | GTAC | 3 cut(s) | 176, 396, 408 |
| RsaNI | GTAC | 3 cut(s) | 175, 395, 407 |
| RseI | CAYNNNNRTG | 2 cut(s) | 269, 377 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 8 cut(s) | 139, 142, 145, 306, 343, 446, 588, 725 |
| Sau3AI | GATC | 6 cut(s) | 75, 160, 193, 532, 642, 728 |
| Sau96I | GGNCC | 3 cut(s) | 386, 440, 704 |
| SetI | ASST | 8 cut(s) | 220, 424, 477, 547, 570, 609, 641, 671 |
| SfaNI | GCATC | 2 cut(s) | 583, 609 |
| SfcI | CTRYAG | 1 cut(s) | 733 |
| SinI | GGWCC | 1 cut(s) | 440 |
| SmaI | CCCGGG | 1 cut(s) | 515 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 269, 377 |
| SrfI | GCCCGGGC | 1 cut(s) | 515 |
| SspMI | CTAG | 1 cut(s) | 546 |
| StyI | CCWWGG | 1 cut(s) | 545 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 2 cut(s) | 220, 671 |
| TaqI | TCGA | 2 cut(s) | 358, 506 |
| TauI | GCSGC | 5 cut(s) | 147, 308, 448, 590, 727 |
| TfiI | GAWTC | 2 cut(s) | 482, 581 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 2 cut(s) | 166, 336 |
| TseI | GCWGC | 3 cut(s) | 138, 141, 342 |
| Tsp45I | GTSAC | 2 cut(s) | 166, 336 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspMI | CCCGGG | 1 cut(s) | 513 |
| TspRI | CASTG | 1 cut(s) | 192 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 1 cut(s) | 440 |
| XceI | RCATGY | 1 cut(s) | 407 |
| XmaI | CCCGGG | 1 cut(s) | 513 |
| XmaJI | CCTAGG | 1 cut(s) | 545 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XmnI | GAANNNNTTC | 1 cut(s) | 486 |
| XspI | CTAG | 1 cut(s) | 546 |
| ZraI | GACGTC | 1 cut(s) | 669 |
| Zsp2I | ATGCAT | 1 cut(s) | 405 |