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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 85 |
| AccII | CGCG | 2 cut(s) | 162, 211 |
| AciI | CCGC | 3 cut(s) | 19, 134, 211 |
| AcsI | RAATTY | 1 cut(s) | 221 |
| AcyI | GRCGYC | 1 cut(s) | 88 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 54, 145 |
| AjnI | CCWGG | 1 cut(s) | 47 |
| AluBI | AGCT | 1 cut(s) | 77 |
| AluI | AGCT | 1 cut(s) | 77 |
| AoxI | GGCC | 3 cut(s) | 23, 97, 183 |
| ApoI | RAATTY | 1 cut(s) | 221 |
| AspLEI | GCGC | 2 cut(s) | 162, 244 |
| AspS9I | GGNCC | 2 cut(s) | 136, 190 |
| AvaII | GGWCC | 2 cut(s) | 136, 190 |
| BciT130I | CCWGG | 1 cut(s) | 49 |
| BfaI | CTAG | 2 cut(s) | 78, 95 |
| BfoI | RGCGCY | 1 cut(s) | 245 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 96 |
| BisI | GCNGC | 1 cut(s) | 19 |
| BlsI | GCNGC | 1 cut(s) | 20 |
| Bme1390I | CCNGG | 1 cut(s) | 49 |
| Bme18I | GGWCC | 2 cut(s) | 136, 190 |
| BmgT120I | GGNCC | 2 cut(s) | 136, 190 |
| BmiI | GGNNCC | 1 cut(s) | 17 |
| BmrFI | CCNGG | 1 cut(s) | 49 |
| BsaHI | GRCGYC | 1 cut(s) | 88 |
| BsaJI | CCNNGG | 2 cut(s) | 47, 186 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 54, 145 |
| Bse1I | ACTGG | 1 cut(s) | 135 |
| BseBI | CCWGG | 1 cut(s) | 49 |
| BseDI | CCNNGG | 2 cut(s) | 47, 186 |
| BseGI | GGATG | 1 cut(s) | 219 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 54, 145 |
| BseNI | ACTGG | 1 cut(s) | 135 |
| Bsh1236I | CGCG | 2 cut(s) | 162, 211 |
| BshFI | GGCC | 3 cut(s) | 25, 99, 185 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 54, 145 |
| BsnI | GGCC | 3 cut(s) | 25, 99, 185 |
| BspACI | CCGC | 3 cut(s) | 19, 134, 211 |
| BspANI | GGCC | 3 cut(s) | 25, 99, 185 |
| BspFNI | CGCG | 2 cut(s) | 162, 211 |
| BspLI | GGNNCC | 1 cut(s) | 17 |
| BsrI | ACTGG | 1 cut(s) | 135 |
| BssECI | CCNNGG | 2 cut(s) | 47, 186 |
| BssNI | GRCGYC | 1 cut(s) | 88 |
| Bst2UI | CCWGG | 1 cut(s) | 49 |
| BstACI | GRCGYC | 1 cut(s) | 88 |
| BstC8I | GCNNGC | 1 cut(s) | 23 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 143 |
| BstF5I | GGATG | 1 cut(s) | 219 |
| BstFNI | CGCG | 2 cut(s) | 162, 211 |
| BstH2I | RGCGCY | 1 cut(s) | 245 |
| BstHHI | GCGC | 2 cut(s) | 162, 244 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 96, 248 |
| BstNI | CCWGG | 1 cut(s) | 49 |
| BstSCI | CCNGG | 1 cut(s) | 47 |
| BstUI | CGCG | 2 cut(s) | 162, 211 |
| BsuRI | GGCC | 3 cut(s) | 25, 99, 185 |
| BtsCI | GGATG | 1 cut(s) | 219 |
| BtsI | GCAGTG | 1 cut(s) | 108 |
| BtsIMutI | CAGTG | 2 cut(s) | 108, 128 |
| Cac8I | GCNNGC | 1 cut(s) | 23 |
| CfoI | GCGC | 2 cut(s) | 162, 244 |
| Cfr13I | GGNCC | 2 cut(s) | 136, 190 |
| CseI | GACGC | 2 cut(s) | 96, 257 |
| CviJI | RGCY | 7 cut(s) | 18, 25, 64, 77, 99, 185, 206 |
| CviKI_1 | RGCY | 7 cut(s) | 18, 25, 64, 77, 99, 185, 206 |
| EciI | GGCGGA | 1 cut(s) | 149 |
| Eco47I | GGWCC | 2 cut(s) | 136, 190 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 143 |
| EcoRII | CCWGG | 1 cut(s) | 47 |
| FaiI | YATR | 4 cut(s) | 13, 116, 120, 268 |
| FblI | GTMKAC | 1 cut(s) | 85 |
| Fnu4HI | GCNGC | 1 cut(s) | 19 |
| FokI | GGATG | 1 cut(s) | 226 |
| Fsp4HI | GCNGC | 1 cut(s) | 19 |
| FspBI | CTAG | 2 cut(s) | 78, 95 |
| GlaI | GCGC | 2 cut(s) | 161, 243 |
| GluI | GCNGC | 1 cut(s) | 19 |
| HaeII | RGCGCY | 1 cut(s) | 245 |
| HaeIII | GGCC | 3 cut(s) | 25, 99, 185 |
| HgaI | GACGC | 2 cut(s) | 96, 257 |
| HhaI | GCGC | 2 cut(s) | 162, 244 |
| Hin1I | GRCGYC | 1 cut(s) | 88 |
| Hin6I | GCGC | 2 cut(s) | 160, 242 |
| HinP1I | GCGC | 2 cut(s) | 160, 242 |
| HincII | GTYRAC | 1 cut(s) | 86 |
| HindII | GTYRAC | 1 cut(s) | 86 |
| Hpy166II | GTNNAC | 1 cut(s) | 86 |
| Hpy188I | TCNGA | 1 cut(s) | 71 |
| Hpy188III | TCNNGA | 1 cut(s) | 193 |
| Hpy8I | GTNNAC | 1 cut(s) | 86 |
| Hpy99I | CGWCG | 1 cut(s) | 90 |
| HpyAV | CCTTC | 1 cut(s) | 175 |
| HpyCH4IV | ACGT | 1 cut(s) | 9 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 96, 248 |
| HpySE526I | ACGT | 1 cut(s) | 9 |
| Hsp92I | GRCGYC | 1 cut(s) | 88 |
| HspAI | GCGC | 2 cut(s) | 160, 242 |
| LmnI | GCTCC | 2 cut(s) | 15, 157 |
| LpnPI | CCDG | 7 cut(s) | 7, 34, 61, 87, 116, 206, 230 |
| MaeI | CTAG | 2 cut(s) | 78, 95 |
| MaeII | ACGT | 1 cut(s) | 9 |
| MluCI | AATT | 1 cut(s) | 221 |
| MmeI | TCCRAC | 2 cut(s) | 63, 94 |
| MnlI | CCTC | 5 cut(s) | 54, 116, 149, 181, 231 |
| MseI | TTAA | 1 cut(s) | 6 |
| MslI | CAYNNNNRTG | 1 cut(s) | 113 |
| MspR9I | CCNGG | 1 cut(s) | 49 |
| MvaI | CCWGG | 1 cut(s) | 49 |
| MvnI | CGCG | 2 cut(s) | 162, 211 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 96, 248 |
| NlaIV | GGNNCC | 1 cut(s) | 17 |
| NmeAIII | GCCGAG | 1 cut(s) | 211 |
| PkrI | GCNGC | 1 cut(s) | 20 |
| Psp6I | CCWGG | 1 cut(s) | 47 |
| PspGI | CCWGG | 1 cut(s) | 47 |
| PspN4I | GGNNCC | 1 cut(s) | 17 |
| PspPI | GGNCC | 2 cut(s) | 136, 190 |
| RseI | CAYNNNNRTG | 1 cut(s) | 113 |
| SalI | GTCGAC | 1 cut(s) | 84 |
| SaqAI | TTAA | 1 cut(s) | 6 |
| SatI | GCNGC | 1 cut(s) | 19 |
| Sau96I | GGNCC | 2 cut(s) | 136, 190 |
| ScrFI | CCNGG | 1 cut(s) | 49 |
| SetI | ASST | 6 cut(s) | 12, 53, 79, 108, 141, 192 |
| SinI | GGWCC | 2 cut(s) | 136, 190 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 113 |
| Sse9I | AATT | 1 cut(s) | 221 |
| SsiI | CCGC | 3 cut(s) | 19, 134, 211 |
| SspMI | CTAG | 2 cut(s) | 78, 95 |
| StyD4I | CCNGG | 1 cut(s) | 47 |
| TaiI | ACGT | 1 cut(s) | 12 |
| TaqI | TCGA | 2 cut(s) | 85, 225 |
| TasI | AATT | 1 cut(s) | 221 |
| TauI | GCSGC | 1 cut(s) | 21 |
| Tru1I | TTAA | 1 cut(s) | 6 |
| Tru9I | TTAA | 1 cut(s) | 6 |
| TscAI | CASTG | 2 cut(s) | 115, 135 |
| TspDTI | ATGAA | 2 cut(s) | 135, 243 |
| TspRI | CASTG | 2 cut(s) | 115, 135 |
| VpaK11BI | GGWCC | 2 cut(s) | 136, 190 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 143 |
| XapI | RAATTY | 1 cut(s) | 221 |
| XmiI | GTMKAC | 1 cut(s) | 85 |
| XspI | CTAG | 2 cut(s) | 78, 95 |