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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 420 |
| Acc65I | GGTACC | 2 cut(s) | 405, 432 |
| AccB1I | GGYRCC | 2 cut(s) | 405, 432 |
| AccBSI | CCGCTC | 1 cut(s) | 218 |
| AccII | CGCG | 1 cut(s) | 299 |
| AclWI | GGATC | 2 cut(s) | 65, 281 |
| AfaI | GTAC | 2 cut(s) | 407, 434 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 156, 394 |
| AgeI | ACCGGT | 1 cut(s) | 141 |
| AgsI | TTSAA | 2 cut(s) | 441, 478 |
| AjnI | CCWGG | 5 cut(s) | 164, 176, 302, 373, 571 |
| AluBI | AGCT | 4 cut(s) | 261, 453, 527, 582 |
| AluI | AGCT | 4 cut(s) | 261, 453, 527, 582 |
| Alw21I | GWGCWC | 2 cut(s) | 129, 373 |
| Alw26I | GTCTC | 1 cut(s) | 199 |
| Alw44I | GTGCAC | 1 cut(s) | 125 |
| AlwI | GGATC | 2 cut(s) | 65, 281 |
| AoxI | GGCC | 4 cut(s) | 167, 320, 465, 542 |
| ApaI | GGGCCC | 1 cut(s) | 469 |
| ApaLI | GTGCAC | 1 cut(s) | 125 |
| ApeKI | GCWGC | 3 cut(s) | 221, 380, 527 |
| AsiGI | ACCGGT | 1 cut(s) | 141 |
| Asp718I | GGTACC | 2 cut(s) | 405, 432 |
| AspLEI | GCGC | 3 cut(s) | 299, 352, 380 |
| AspS9I | GGNCC | 5 cut(s) | 180, 240, 465, 466, 542 |
| AsuHPI | GGTGA | 2 cut(s) | 72, 437 |
| AvaII | GGWCC | 2 cut(s) | 180, 240 |
| BaeGI | GKGCMC | 2 cut(s) | 129, 469 |
| BanI | GGYRCC | 2 cut(s) | 405, 432 |
| BanII | GRGCYC | 1 cut(s) | 469 |
| Bbv12I | GWGCWC | 2 cut(s) | 129, 373 |
| BbvI | GCAGC | 3 cut(s) | 233, 392, 514 |
| BciT130I | CCWGG | 5 cut(s) | 166, 178, 304, 375, 573 |
| BclI | TGATCA | 1 cut(s) | 92 |
| BcoDI | GTCTC | 1 cut(s) | 199 |
| BfaI | CTAG | 2 cut(s) | 268, 600 |
| BfmI | CTRYAG | 1 cut(s) | 197 |
| BfuAI | ACCTGC | 1 cut(s) | 420 |
| BisI | GCNGC | 5 cut(s) | 219, 222, 225, 381, 528 |
| BlpI | GCTNAGC | 1 cut(s) | 531 |
| BlsI | GCNGC | 5 cut(s) | 220, 223, 226, 382, 529 |
| Bme1390I | CCNGG | 5 cut(s) | 166, 178, 304, 375, 573 |
| Bme18I | GGWCC | 2 cut(s) | 180, 240 |
| BmgT120I | GGNCC | 5 cut(s) | 180, 240, 465, 466, 542 |
| BmiI | GGNNCC | 5 cut(s) | 161, 182, 407, 434, 467 |
| BmrFI | CCNGG | 5 cut(s) | 166, 178, 304, 375, 573 |
| BmrI | ACTGGG | 1 cut(s) | 82 |
| BmsI | GCATC | 2 cut(s) | 224, 416 |
| BmuI | ACTGGG | 1 cut(s) | 82 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 531 |
| BsaJI | CCNNGG | 5 cut(s) | 164, 176, 323, 551, 571 |
| BsaWI | WCCGGW | 1 cut(s) | 141 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 156, 394 |
| Bse118I | RCCGGY | 2 cut(s) | 141, 394 |
| Bse1I | ACTGG | 2 cut(s) | 88, 170 |
| Bse3DI | GCAATG | 1 cut(s) | 368 |
| BseBI | CCWGG | 5 cut(s) | 166, 178, 304, 375, 573 |
| BseDI | CCNNGG | 5 cut(s) | 164, 176, 323, 551, 571 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 156, 394 |
| BseMI | GCAATG | 1 cut(s) | 368 |
| BseMII | CTCAG | 2 cut(s) | 38, 545 |
| BseNI | ACTGG | 2 cut(s) | 88, 170 |
| BseSI | GKGCMC | 2 cut(s) | 129, 469 |
| BseXI | GCAGC | 3 cut(s) | 233, 392, 514 |
| BseYI | CCCAGC | 3 cut(s) | 105, 151, 346 |
| Bsh1236I | CGCG | 1 cut(s) | 299 |
| BshFI | GGCC | 4 cut(s) | 169, 322, 467, 544 |
| BshNI | GGYRCC | 2 cut(s) | 405, 432 |
| BshTI | ACCGGT | 1 cut(s) | 141 |
| BsiHKAI | GWGCWC | 2 cut(s) | 129, 373 |
| BsiSI | CCGG | 2 cut(s) | 142, 395 |
| BslFI | GGGAC | 2 cut(s) | 166, 553 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 156, 394 |
| BsmAI | GTCTC | 1 cut(s) | 199 |
| BsmFI | GGGAC | 2 cut(s) | 166, 553 |
| BsnI | GGCC | 4 cut(s) | 169, 322, 467, 544 |
| Bsp120I | GGGCCC | 1 cut(s) | 465 |
| Bsp1286I | GDGCHC | 3 cut(s) | 129, 373, 469 |
| Bsp143I | GATC | 3 cut(s) | 57, 92, 273 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 531 |
| BspANI | GGCC | 4 cut(s) | 169, 322, 467, 544 |
| BspCNI | CTCAG | 2 cut(s) | 39, 544 |
| BspFNI | CGCG | 1 cut(s) | 299 |
| BspLI | GGNNCC | 5 cut(s) | 161, 182, 407, 434, 467 |
| BspMI | ACCTGC | 1 cut(s) | 420 |
| BspPI | GGATC | 2 cut(s) | 65, 281 |
| BspT107I | GGYRCC | 2 cut(s) | 405, 432 |
| BsrBI | CCGCTC | 1 cut(s) | 218 |
| BsrDI | GCAATG | 1 cut(s) | 368 |
| BsrFI | RCCGGY | 2 cut(s) | 141, 394 |
| BsrI | ACTGG | 2 cut(s) | 88, 170 |
| BssAI | RCCGGY | 2 cut(s) | 141, 394 |
| BssECI | CCNNGG | 5 cut(s) | 164, 176, 323, 551, 571 |
| BssMI | GATC | 3 cut(s) | 57, 92, 273 |
| Bst2UI | CCWGG | 5 cut(s) | 166, 178, 304, 375, 573 |
| Bst4CI | ACNGT | 4 cut(s) | 31, 124, 201, 437 |
| BstC8I | GCNNGC | 4 cut(s) | 53, 263, 359, 385 |
| BstDEI | CTNAG | 2 cut(s) | 47, 531 |
| BstFNI | CGCG | 1 cut(s) | 299 |
| BstHHI | GCGC | 3 cut(s) | 299, 352, 380 |
| BstKTI | GATC | 3 cut(s) | 60, 95, 276 |
| BstMAI | GTCTC | 1 cut(s) | 199 |
| BstMBI | GATC | 3 cut(s) | 57, 92, 273 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 224, 347, 358, 363, 377, 384, 541, 588 |
| BstNI | CCWGG | 5 cut(s) | 166, 178, 304, 375, 573 |
| BstSCI | CCNGG | 5 cut(s) | 164, 176, 302, 373, 571 |
| BstSFI | CTRYAG | 1 cut(s) | 197 |
| BstSLI | GKGCMC | 2 cut(s) | 129, 469 |
| BstUI | CGCG | 1 cut(s) | 299 |
| BstV1I | GCAGC | 3 cut(s) | 233, 392, 514 |
| BstX2I | RGATCY | 1 cut(s) | 273 |
| BstYI | RGATCY | 1 cut(s) | 273 |
| BsuRI | GGCC | 4 cut(s) | 169, 322, 467, 544 |
| BtsIMutI | CAGTG | 3 cut(s) | 44, 95, 206 |
| BveI | ACCTGC | 1 cut(s) | 420 |
| Cac8I | GCNNGC | 4 cut(s) | 53, 263, 359, 385 |
| CfoI | GCGC | 3 cut(s) | 299, 352, 380 |
| Cfr10I | RCCGGY | 2 cut(s) | 141, 394 |
| Cfr13I | GGNCC | 5 cut(s) | 180, 240, 465, 466, 542 |
| Csp6I | GTAC | 2 cut(s) | 406, 433 |
| CspAI | ACCGGT | 1 cut(s) | 141 |
| CviAII | CATG | 1 cut(s) | 21 |
| CviQI | GTAC | 2 cut(s) | 406, 433 |
| DdeI | CTNAG | 2 cut(s) | 47, 531 |
| DpnI | GATC | 3 cut(s) | 59, 94, 275 |
| DpnII | GATC | 3 cut(s) | 57, 92, 273 |
| EciI | GGCGGA | 4 cut(s) | 54, 70, 413, 520 |
| Eco24I | GRGCYC | 1 cut(s) | 469 |
| Eco47I | GGWCC | 2 cut(s) | 180, 240 |
| EcoO109I | RGGNCCY | 2 cut(s) | 180, 240 |
| EcoRII | CCWGG | 5 cut(s) | 164, 176, 302, 373, 571 |
| EcoT38I | GRGCYC | 1 cut(s) | 469 |
| FaeI | CATG | 1 cut(s) | 24 |
| FaiI | YATR | 4 cut(s) | 22, 253, 257, 421 |
| FaqI | GGGAC | 2 cut(s) | 166, 553 |
| FatI | CATG | 1 cut(s) | 20 |
| FauI | CCCGC | 1 cut(s) | 28 |
| FbaI | TGATCA | 1 cut(s) | 92 |
| Fnu4HI | GCNGC | 5 cut(s) | 219, 222, 225, 381, 528 |
| FriOI | GRGCYC | 1 cut(s) | 469 |
| Fsp4HI | GCNGC | 5 cut(s) | 219, 222, 225, 381, 528 |
| FspBI | CTAG | 2 cut(s) | 268, 600 |
| GlaI | GCGC | 3 cut(s) | 298, 351, 379 |
| GluI | GCNGC | 5 cut(s) | 219, 222, 225, 381, 528 |
| GsaI | CCCAGC | 3 cut(s) | 109, 155, 350 |
| HaeIII | GGCC | 4 cut(s) | 169, 322, 467, 544 |
| HapII | CCGG | 2 cut(s) | 142, 395 |
| HhaI | GCGC | 3 cut(s) | 299, 352, 380 |
| Hin1II | CATG | 1 cut(s) | 24 |
| Hin6I | GCGC | 3 cut(s) | 297, 350, 378 |
| HinP1I | GCGC | 3 cut(s) | 297, 350, 378 |
| HincII | GTYRAC | 3 cut(s) | 102, 120, 147 |
| HindII | GTYRAC | 3 cut(s) | 102, 120, 147 |
| HinfI | GANTC | 2 cut(s) | 207, 508 |
| HpaI | GTTAAC | 2 cut(s) | 120, 147 |
| HpaII | CCGG | 2 cut(s) | 142, 395 |
| HphI | GGTGA | 2 cut(s) | 72, 437 |
| Hpy166II | GTNNAC | 4 cut(s) | 102, 120, 127, 147 |
| Hpy188I | TCNGA | 1 cut(s) | 233 |
| Hpy188III | TCNNGA | 2 cut(s) | 292, 475 |
| Hpy8I | GTNNAC | 4 cut(s) | 102, 120, 127, 147 |
| HpyCH4III | ACNGT | 4 cut(s) | 31, 124, 201, 437 |
| HpyCH4IV | ACGT | 1 cut(s) | 98 |
| HpyCH4V | TGCA | 4 cut(s) | 127, 237, 366, 429 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 224, 347, 358, 363, 377, 384, 541, 588 |
| HpyF3I | CTNAG | 2 cut(s) | 47, 531 |
| HpySE526I | ACGT | 1 cut(s) | 98 |
| Hsp92II | CATG | 1 cut(s) | 24 |
| HspAI | GCGC | 3 cut(s) | 297, 350, 378 |
| KpnI | GGTACC | 2 cut(s) | 409, 436 |
| Ksp22I | TGATCA | 1 cut(s) | 92 |
| KspAI | GTTAAC | 2 cut(s) | 120, 147 |
| Kzo9I | GATC | 3 cut(s) | 57, 92, 273 |
| Lsp1109I | GCAGC | 3 cut(s) | 233, 392, 514 |
| LweI | GCATC | 2 cut(s) | 224, 416 |
| MaeI | CTAG | 2 cut(s) | 268, 600 |
| MaeII | ACGT | 1 cut(s) | 98 |
| MaeIII | GTNAC | 1 cut(s) | 509 |
| MalI | GATC | 3 cut(s) | 59, 94, 275 |
| MbiI | CCGCTC | 1 cut(s) | 218 |
| MboI | GATC | 3 cut(s) | 57, 92, 273 |
| MboII | GAAGA | 1 cut(s) | 268 |
| MflI | RGATCY | 1 cut(s) | 273 |
| MhlI | GDGCHC | 3 cut(s) | 129, 373, 469 |
| MlyI | GAGTC | 2 cut(s) | 201, 517 |
| MmeI | TCCRAC | 1 cut(s) | 473 |
| MnlI | CCTC | 7 cut(s) | 88, 239, 253, 318, 451, 482, 546 |
| MseI | TTAA | 3 cut(s) | 113, 119, 146 |
| MslI | CAYNNNNRTG | 1 cut(s) | 424 |
| MspA1I | CMGCKG | 2 cut(s) | 527, 591 |
| MspI | CCGG | 2 cut(s) | 142, 395 |
| MspR9I | CCNGG | 5 cut(s) | 166, 178, 304, 375, 573 |
| MvaI | CCWGG | 5 cut(s) | 166, 178, 304, 375, 573 |
| MvnI | CGCG | 1 cut(s) | 299 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 224, 347, 358, 363, 377, 384, 541, 588 |
| NdeII | GATC | 3 cut(s) | 57, 92, 273 |
| NlaIII | CATG | 1 cut(s) | 24 |
| NlaIV | GGNNCC | 5 cut(s) | 161, 182, 407, 434, 467 |
| NmeAIII | GCCGAG | 1 cut(s) | 348 |
| NmuCI | GTSAC | 1 cut(s) | 509 |
| PinAI | ACCGGT | 1 cut(s) | 141 |
| PkrI | GCNGC | 5 cut(s) | 220, 223, 226, 382, 529 |
| PleI | GAGTC | 2 cut(s) | 201, 516 |
| PpsI | GAGTC | 2 cut(s) | 201, 516 |
| PpuMI | RGGWCCY | 2 cut(s) | 180, 240 |
| Psp5II | RGGWCCY | 2 cut(s) | 180, 240 |
| Psp6I | CCWGG | 5 cut(s) | 164, 176, 302, 373, 571 |
| PspFI | CCCAGC | 3 cut(s) | 105, 151, 346 |
| PspGI | CCWGG | 5 cut(s) | 164, 176, 302, 373, 571 |
| PspN4I | GGNNCC | 5 cut(s) | 161, 182, 407, 434, 467 |
| PspOMI | GGGCCC | 1 cut(s) | 465 |
| PspPI | GGNCC | 5 cut(s) | 180, 240, 465, 466, 542 |
| PspPPI | RGGWCCY | 2 cut(s) | 180, 240 |
| PsuI | RGATCY | 1 cut(s) | 273 |
| PvuII | CAGCTG | 1 cut(s) | 527 |
| RsaI | GTAC | 2 cut(s) | 407, 434 |
| RsaNI | GTAC | 2 cut(s) | 406, 433 |
| RseI | CAYNNNNRTG | 1 cut(s) | 424 |
| SaqAI | TTAA | 3 cut(s) | 113, 119, 146 |
| SatI | GCNGC | 5 cut(s) | 219, 222, 225, 381, 528 |
| Sau3AI | GATC | 3 cut(s) | 57, 92, 273 |
| Sau96I | GGNCC | 5 cut(s) | 180, 240, 465, 466, 542 |
| SchI | GAGTC | 2 cut(s) | 201, 517 |
| ScrFI | CCNGG | 5 cut(s) | 166, 178, 304, 375, 573 |
| SduI | GDGCHC | 3 cut(s) | 129, 373, 469 |
| SfaNI | GCATC | 2 cut(s) | 224, 416 |
| SfcI | CTRYAG | 1 cut(s) | 197 |
| SgrAI | CRCCGGYG | 1 cut(s) | 394 |
| SinI | GGWCC | 2 cut(s) | 180, 240 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 424 |
| SspMI | CTAG | 2 cut(s) | 268, 600 |
| StyD4I | CCNGG | 5 cut(s) | 164, 176, 302, 373, 571 |
| TaaI | ACNGT | 4 cut(s) | 31, 124, 201, 437 |
| TaiI | ACGT | 1 cut(s) | 101 |
| TaqI | TCGA | 1 cut(s) | 291 |
| TauI | GCSGC | 2 cut(s) | 221, 227 |
| Tru1I | TTAA | 3 cut(s) | 113, 119, 146 |
| Tru9I | TTAA | 3 cut(s) | 113, 119, 146 |
| TscAI | CASTG | 3 cut(s) | 51, 95, 206 |
| TseFI | GTSAC | 1 cut(s) | 509 |
| TseI | GCWGC | 3 cut(s) | 221, 380, 527 |
| Tsp45I | GTSAC | 1 cut(s) | 509 |
| TspRI | CASTG | 3 cut(s) | 51, 95, 206 |
| VneI | GTGCAC | 1 cut(s) | 125 |
| VpaK11BI | GGWCC | 2 cut(s) | 180, 240 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 579 |
| XspI | CTAG | 2 cut(s) | 268, 600 |