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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 48 |
| AciI | CCGC | 2 cut(s) | 208, 234 |
| AcoI | YGGCCR | 1 cut(s) | 90 |
| AcuI | CTGAAG | 1 cut(s) | 140 |
| AfaI | GTAC | 3 cut(s) | 175, 205, 232 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 31 |
| AluBI | AGCT | 1 cut(s) | 10 |
| AluI | AGCT | 1 cut(s) | 10 |
| Ama87I | CYCGRG | 1 cut(s) | 246 |
| AoxI | GGCC | 3 cut(s) | 69, 75, 90 |
| AspLEI | GCGC | 2 cut(s) | 130, 154 |
| AspS9I | GGNCC | 2 cut(s) | 76, 264 |
| AvaI | CYCGRG | 1 cut(s) | 246 |
| AvaII | GGWCC | 1 cut(s) | 264 |
| BalI | TGGCCA | 1 cut(s) | 92 |
| BfoI | RGCGCY | 1 cut(s) | 131 |
| Bme18I | GGWCC | 1 cut(s) | 264 |
| BmeT110I | CYCGRG | 1 cut(s) | 246 |
| BmgT120I | GGNCC | 2 cut(s) | 76, 264 |
| BmiI | GGNNCC | 1 cut(s) | 265 |
| BpmI | CTGGAG | 1 cut(s) | 152 |
| BsaJI | CCNNGG | 1 cut(s) | 72 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 31 |
| Bse118I | RCCGGY | 1 cut(s) | 192 |
| BseDI | CCNNGG | 1 cut(s) | 72 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 31 |
| BseYI | CCCAGC | 1 cut(s) | 148 |
| Bsh1236I | CGCG | 1 cut(s) | 48 |
| BshFI | GGCC | 3 cut(s) | 71, 77, 92 |
| BsiHKCI | CYCGRG | 1 cut(s) | 246 |
| BsiSI | CCGG | 1 cut(s) | 193 |
| BslFI | GGGAC | 1 cut(s) | 277 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 31 |
| BsmFI | GGGAC | 1 cut(s) | 277 |
| BsnI | GGCC | 3 cut(s) | 71, 77, 92 |
| BsoBI | CYCGRG | 1 cut(s) | 246 |
| BspACI | CCGC | 2 cut(s) | 208, 234 |
| BspANI | GGCC | 3 cut(s) | 71, 77, 92 |
| BspFNI | CGCG | 1 cut(s) | 48 |
| BspLI | GGNNCC | 1 cut(s) | 265 |
| BsrFI | RCCGGY | 1 cut(s) | 192 |
| BssAI | RCCGGY | 1 cut(s) | 192 |
| BssECI | CCNNGG | 1 cut(s) | 72 |
| BstC8I | GCNNGC | 2 cut(s) | 12, 161 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 29 |
| BstFNI | CGCG | 1 cut(s) | 48 |
| BstH2I | RGCGCY | 1 cut(s) | 131 |
| BstHHI | GCGC | 2 cut(s) | 130, 154 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 134, 149, 160 |
| BstUI | CGCG | 1 cut(s) | 48 |
| BsuRI | GGCC | 3 cut(s) | 71, 77, 92 |
| BtsIMutI | CAGTG | 1 cut(s) | 95 |
| Cac8I | GCNNGC | 2 cut(s) | 12, 161 |
| CfoI | GCGC | 2 cut(s) | 130, 154 |
| Cfr10I | RCCGGY | 1 cut(s) | 192 |
| Cfr13I | GGNCC | 2 cut(s) | 76, 264 |
| Csp6I | GTAC | 3 cut(s) | 174, 204, 231 |
| CviAII | CATG | 1 cut(s) | 297 |
| CviJI | RGCY | 4 cut(s) | 10, 71, 77, 92 |
| CviKI_1 | RGCY | 4 cut(s) | 10, 71, 77, 92 |
| CviQI | GTAC | 3 cut(s) | 174, 204, 231 |
| EaeI | YGGCCR | 1 cut(s) | 90 |
| Eco47I | GGWCC | 1 cut(s) | 264 |
| Eco57I | CTGAAG | 1 cut(s) | 140 |
| Eco88I | CYCGRG | 1 cut(s) | 246 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 29 |
| FaeI | CATG | 1 cut(s) | 300 |
| FaiI | YATR | 3 cut(s) | 6, 219, 298 |
| FaqI | GGGAC | 1 cut(s) | 277 |
| FatI | CATG | 1 cut(s) | 296 |
| FauI | CCCGC | 1 cut(s) | 215 |
| GlaI | GCGC | 2 cut(s) | 129, 153 |
| GsaI | CCCAGC | 1 cut(s) | 152 |
| GsuI | CTGGAG | 1 cut(s) | 152 |
| HaeII | RGCGCY | 1 cut(s) | 131 |
| HaeIII | GGCC | 3 cut(s) | 71, 77, 92 |
| HapII | CCGG | 1 cut(s) | 193 |
| HhaI | GCGC | 2 cut(s) | 130, 154 |
| Hin1II | CATG | 1 cut(s) | 300 |
| Hin6I | GCGC | 2 cut(s) | 128, 152 |
| HinP1I | GCGC | 2 cut(s) | 128, 152 |
| HinfI | GANTC | 1 cut(s) | 108 |
| HpaII | CCGG | 1 cut(s) | 193 |
| Hpy188III | TCNNGA | 1 cut(s) | 105 |
| Hpy99I | CGWCG | 1 cut(s) | 116 |
| HpyAV | CCTTC | 1 cut(s) | 118 |
| HpyCH4IV | ACGT | 1 cut(s) | 181 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 134, 149, 160 |
| HpySE526I | ACGT | 1 cut(s) | 181 |
| Hsp92II | CATG | 1 cut(s) | 300 |
| HspAI | GCGC | 2 cut(s) | 128, 152 |
| LpnPI | CCDG | 6 cut(s) | 25, 116, 162, 190, 206, 288 |
| MaeII | ACGT | 1 cut(s) | 181 |
| MboII | GAAGA | 1 cut(s) | 133 |
| MlsI | TGGCCA | 1 cut(s) | 92 |
| MluNI | TGGCCA | 1 cut(s) | 92 |
| MnlI | CCTC | 5 cut(s) | 35, 67, 127, 280, 289 |
| Mox20I | TGGCCA | 1 cut(s) | 92 |
| MscI | TGGCCA | 1 cut(s) | 92 |
| MslI | CAYNNNNRTG | 1 cut(s) | 98 |
| Msp20I | TGGCCA | 1 cut(s) | 92 |
| MspA1I | CMGCKG | 1 cut(s) | 236 |
| MspI | CCGG | 1 cut(s) | 193 |
| MvnI | CGCG | 1 cut(s) | 48 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 134, 149, 160 |
| NlaIII | CATG | 1 cut(s) | 300 |
| NlaIV | GGNNCC | 1 cut(s) | 265 |
| NmeAIII | GCCGAG | 1 cut(s) | 97 |
| PfeI | GAWTC | 1 cut(s) | 108 |
| PflFI | GACNNNGTC | 1 cut(s) | 183 |
| PspFI | CCCAGC | 1 cut(s) | 148 |
| PspN4I | GGNNCC | 1 cut(s) | 265 |
| PspPI | GGNCC | 2 cut(s) | 76, 264 |
| PsyI | GACNNNGTC | 1 cut(s) | 183 |
| RsaI | GTAC | 3 cut(s) | 175, 205, 232 |
| RsaNI | GTAC | 3 cut(s) | 174, 204, 231 |
| RseI | CAYNNNNRTG | 1 cut(s) | 98 |
| Sau96I | GGNCC | 2 cut(s) | 76, 264 |
| SetI | ASST | 4 cut(s) | 12, 27, 184, 272 |
| SinI | GGWCC | 1 cut(s) | 264 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 98 |
| SsiI | CCGC | 2 cut(s) | 208, 234 |
| TaiI | ACGT | 1 cut(s) | 184 |
| TaqI | TCGA | 2 cut(s) | 111, 281 |
| TfiI | GAWTC | 1 cut(s) | 108 |
| TscAI | CASTG | 1 cut(s) | 102 |
| TspRI | CASTG | 1 cut(s) | 102 |
| Tth111I | GACNNNGTC | 1 cut(s) | 183 |
| VpaK11BI | GGWCC | 1 cut(s) | 264 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 29 |