transposition, RNA-mediated
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 595, 875 |
| Acc36I | ACCTGC | 1 cut(s) | 1948 |
| Acc65I | GGTACC | 1 cut(s) | 487 |
| AccB1I | GGYRCC | 2 cut(s) | 487, 740 |
| AccBSI | CCGCTC | 5 cut(s) | 58, 79, 585, 697, 1284 |
| AccI | GTMKAC | 1 cut(s) | 1857 |
| AccII | CGCG | 2 cut(s) | 421, 1053 |
| AclWI | GGATC | 6 cut(s) | 193, 206, 796, 809, 1843, 1856 |
| AcoI | YGGCCR | 1 cut(s) | 342 |
| AcsI | RAATTY | 3 cut(s) | 359, 1193, 1395 |
| AcuI | CTGAAG | 2 cut(s) | 392, 1121 |
| AcvI | CACGTG | 1 cut(s) | 1676 |
| AcyI | GRCGYC | 3 cut(s) | 741, 927, 1818 |
| AdeI | CACNNNGTG | 1 cut(s) | 1026 |
| AfaI | GTAC | 4 cut(s) | 462, 489, 1334, 1563 |
| AfeI | AGCGCT | 1 cut(s) | 1667 |
| AgeI | ACCGGT | 1 cut(s) | 1329 |
| AgsI | TTSAA | 9 cut(s) | 496, 533, 895, 922, 1009, 1087, 1523, 1558, 1888 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1864 |
| AjnI | CCWGG | 5 cut(s) | 183, 781, 797, 1022, 1290 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 141, 173 |
| AluBI | AGCT | 7 cut(s) | 97, 221, 280, 404, 508, 637, 1729 |
| AluI | AGCT | 7 cut(s) | 97, 221, 280, 404, 508, 637, 1729 |
| Alw21I | GWGCWC | 3 cut(s) | 428, 1633, 1779 |
| Alw26I | GTCTC | 4 cut(s) | 42, 666, 1530, 1653 |
| AlwI | GGATC | 6 cut(s) | 193, 206, 796, 809, 1843, 1856 |
| Aor51HI | AGCGCT | 1 cut(s) | 1667 |
| AoxI | GGCC | 9 cut(s) | 25, 43, 186, 342, 729, 1106, 1356, 1603, 1900 |
| ApeKI | GCWGC | 5 cut(s) | 82, 240, 858, 1233, 1249 |
| ApoI | RAATTY | 3 cut(s) | 359, 1193, 1395 |
| AsiGI | ACCGGT | 1 cut(s) | 1329 |
| Asp718I | GGTACC | 1 cut(s) | 487 |
| AspA2I | CCTAGG | 1 cut(s) | 626 |
| AspLEI | GCGC | 5 cut(s) | 330, 382, 743, 751, 1668 |
| AsuC2I | CCSGG | 1 cut(s) | 728 |
| AsuHPI | GGTGA | 8 cut(s) | 91, 155, 256, 439, 895, 1012, 1112, 1630 |
| AvaII | GGWCC | 8 cut(s) | 259, 521, 597, 662, 785, 1719, 1925, 1937 |
| AvrII | CCTAGG | 1 cut(s) | 626 |
| BalI | TGGCCA | 1 cut(s) | 344 |
| BamHI | GGATCC | 3 cut(s) | 198, 801, 1848 |
| BanI | GGYRCC | 2 cut(s) | 487, 740 |
| BanII | GRGCYC | 1 cut(s) | 1752 |
| BauI | CACGAG | 1 cut(s) | 1469 |
| BbrPI | CACGTG | 1 cut(s) | 1676 |
| Bbv12I | GWGCWC | 3 cut(s) | 428, 1633, 1779 |
| BbvCI | CCTCAGC | 1 cut(s) | 222 |
| BbvI | GCAGC | 5 cut(s) | 94, 252, 845, 1245, 1261 |
| BccI | CCATC | 7 cut(s) | 452, 475, 662, 821, 1616, 1625, 1911 |
| BceAI | ACGGC | 1 cut(s) | 871 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1610, 1644 |
| BciT130I | CCWGG | 5 cut(s) | 185, 783, 799, 1024, 1292 |
| BclI | TGATCA | 2 cut(s) | 1258, 1581 |
| BcnI | CCSGG | 1 cut(s) | 728 |
| BcoDI | GTCTC | 4 cut(s) | 42, 666, 1530, 1653 |
| BfaI | CTAG | 4 cut(s) | 234, 287, 429, 627 |
| BfmI | CTRYAG | 1 cut(s) | 216 |
| BfoI | RGCGCY | 4 cut(s) | 383, 744, 752, 1669 |
| BfuAI | ACCTGC | 1 cut(s) | 1948 |
| BlnI | CCTAGG | 1 cut(s) | 626 |
| BlpI | GCTNAGC | 1 cut(s) | 228 |
| Bme1390I | CCNGG | 6 cut(s) | 185, 728, 783, 799, 1024, 1292 |
| Bme18I | GGWCC | 8 cut(s) | 259, 521, 597, 662, 785, 1719, 1925, 1937 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1864 |
| BmrFI | CCNGG | 6 cut(s) | 185, 728, 783, 799, 1024, 1292 |
| BmrI | ACTGGG | 1 cut(s) | 1039 |
| BmsI | GCATC | 4 cut(s) | 152, 660, 690, 1617 |
| BmuI | ACTGGG | 1 cut(s) | 1039 |
| BpmI | CTGGAG | 1 cut(s) | 713 |
| Bpu10I | CCTNAGC | 1 cut(s) | 222 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 228 |
| BpuEI | CTTGAG | 2 cut(s) | 968, 1337 |
| BpuMI | CCSGG | 1 cut(s) | 728 |
| Bsa29I | ATCGAT | 1 cut(s) | 1620 |
| BsaAI | YACGTR | 1 cut(s) | 1676 |
| BsaHI | GRCGYC | 3 cut(s) | 741, 927, 1818 |
| BsaWI | WCCGGW | 4 cut(s) | 195, 250, 1329, 1934 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 121, 151, 548, 578 |
| Bse118I | RCCGGY | 2 cut(s) | 449, 1329 |
| Bse1I | ACTGG | 4 cut(s) | 189, 462, 696, 1045 |
| Bse3DI | GCAATG | 1 cut(s) | 1507 |
| BseBI | CCWGG | 5 cut(s) | 185, 783, 799, 1024, 1292 |
| BseCI | ATCGAT | 1 cut(s) | 1620 |
| BseGI | GGATG | 8 cut(s) | 167, 651, 705, 813, 1040, 1135, 1176, 1322 |
| BseMI | GCAATG | 1 cut(s) | 1507 |
| BseMII | CTCAG | 4 cut(s) | 213, 242, 597, 1928 |
| BseNI | ACTGG | 4 cut(s) | 189, 462, 696, 1045 |
| BseRI | GAGGAG | 3 cut(s) | 983, 1784, 1787 |
| BseXI | GCAGC | 5 cut(s) | 94, 252, 845, 1245, 1261 |
| BseYI | CCCAGC | 4 cut(s) | 40, 170, 400, 745 |
| BsgI | GTGCAG | 1 cut(s) | 275 |
| Bsh1236I | CGCG | 2 cut(s) | 421, 1053 |
| BshFI | GGCC | 9 cut(s) | 27, 45, 188, 344, 731, 1108, 1358, 1605, 1902 |
| BshNI | GGYRCC | 2 cut(s) | 487, 740 |
| BshTI | ACCGGT | 1 cut(s) | 1329 |
| BshVI | ATCGAT | 1 cut(s) | 1620 |
| BsiHKAI | GWGCWC | 3 cut(s) | 428, 1633, 1779 |
| BsiSI | CCGG | 8 cut(s) | 28, 196, 251, 450, 727, 1330, 1935, 1968 |
| BslFI | GGGAC | 4 cut(s) | 534, 608, 675, 1107 |
| BsmAI | GTCTC | 4 cut(s) | 42, 666, 1530, 1653 |
| BsmBI | CGTCTC | 1 cut(s) | 1530 |
| BsmFI | GGGAC | 4 cut(s) | 534, 608, 675, 1107 |
| BsnI | GGCC | 9 cut(s) | 27, 45, 188, 344, 731, 1108, 1358, 1605, 1902 |
| Bsp1286I | GDGCHC | 4 cut(s) | 428, 1633, 1752, 1779 |
| Bsp143I | GATC | 8 cut(s) | 198, 801, 1054, 1258, 1581, 1812, 1848, 1955 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 228 |
| Bsp19I | CCATGG | 1 cut(s) | 1156 |
| Bsp68I | TCGCGA | 1 cut(s) | 1053 |
| BspANI | GGCC | 9 cut(s) | 27, 45, 188, 344, 731, 1108, 1358, 1605, 1902 |
| BspCNI | CTCAG | 4 cut(s) | 214, 241, 598, 1927 |
| BspDI | ATCGAT | 1 cut(s) | 1620 |
| BspFNI | CGCG | 2 cut(s) | 421, 1053 |
| BspMI | ACCTGC | 1 cut(s) | 1948 |
| BspPI | GGATC | 6 cut(s) | 193, 206, 796, 809, 1843, 1856 |
| BspT107I | GGYRCC | 2 cut(s) | 487, 740 |
| BsrBI | CCGCTC | 5 cut(s) | 58, 79, 585, 697, 1284 |
| BsrDI | GCAATG | 1 cut(s) | 1507 |
| BsrFI | RCCGGY | 2 cut(s) | 449, 1329 |
| BsrI | ACTGG | 4 cut(s) | 189, 462, 696, 1045 |
| BssAI | RCCGGY | 2 cut(s) | 449, 1329 |
| BssMI | GATC | 8 cut(s) | 198, 801, 1054, 1258, 1581, 1812, 1848, 1955 |
| BssNI | GRCGYC | 3 cut(s) | 741, 927, 1818 |
| BssSI | CACGAG | 1 cut(s) | 1469 |
| BssT1I | CCWWGG | 6 cut(s) | 35, 626, 1156, 1272, 1599, 1689 |
| Bst2BI | CACGAG | 1 cut(s) | 1469 |
| Bst2UI | CCWGG | 5 cut(s) | 185, 783, 799, 1024, 1292 |
| Bst4CI | ACNGT | 2 cut(s) | 1304, 1348 |
| Bst6I | CTCTTC | 1 cut(s) | 1208 |
| BstACI | GRCGYC | 3 cut(s) | 741, 927, 1818 |
| BstBAI | YACGTR | 1 cut(s) | 1676 |
| BstC8I | GCNNGC | 6 cut(s) | 282, 413, 739, 810, 1077, 1247 |
| BstDEI | CTNAG | 5 cut(s) | 222, 228, 606, 1725, 1914 |
| BstDSI | CCRYGG | 2 cut(s) | 1156, 1424 |
| BstEII | GGTNACC | 1 cut(s) | 1277 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 986 |
| BstF5I | GGATG | 8 cut(s) | 167, 651, 705, 813, 1040, 1135, 1176, 1322 |
| BstFNI | CGCG | 2 cut(s) | 421, 1053 |
| BstH2I | RGCGCY | 4 cut(s) | 383, 744, 752, 1669 |
| BstHHI | GCGC | 5 cut(s) | 330, 382, 743, 751, 1668 |
| BstKTI | GATC | 8 cut(s) | 201, 804, 1057, 1261, 1584, 1815, 1851, 1958 |
| BstMAI | GTCTC | 4 cut(s) | 42, 666, 1530, 1653 |
| BstMBI | GATC | 8 cut(s) | 198, 801, 1054, 1258, 1581, 1812, 1848, 1955 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 85, 94, 121, 162, 171, 401, 1242, 1707 |
| BstNI | CCWGG | 5 cut(s) | 185, 783, 799, 1024, 1292 |
| BstNSI | RCATGY | 2 cut(s) | 55, 812 |
| BstPI | GGTNACC | 1 cut(s) | 1277 |
| BstSCI | CCNGG | 6 cut(s) | 183, 726, 781, 797, 1022, 1290 |
| BstSFI | CTRYAG | 1 cut(s) | 216 |
| BstUI | CGCG | 2 cut(s) | 421, 1053 |
| BstV1I | GCAGC | 5 cut(s) | 94, 252, 845, 1245, 1261 |
| BstX2I | RGATCY | 3 cut(s) | 198, 801, 1848 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 691 |
| BstYI | RGATCY | 3 cut(s) | 198, 801, 1848 |
| Bsu15I | ATCGAT | 1 cut(s) | 1620 |
| BsuRI | GGCC | 9 cut(s) | 27, 45, 188, 344, 731, 1108, 1358, 1605, 1902 |
| BsuTUI | ATCGAT | 1 cut(s) | 1620 |
| BtgI | CCRYGG | 2 cut(s) | 1156, 1424 |
| BtsCI | GGATG | 8 cut(s) | 167, 651, 705, 813, 1040, 1135, 1176, 1322 |
| BtsI | GCAGTG | 2 cut(s) | 792, 1344 |
| BtsIMutI | CAGTG | 6 cut(s) | 792, 1052, 1344, 1344, 1388, 1845 |
| BtuMI | TCGCGA | 1 cut(s) | 1053 |
| BveI | ACCTGC | 1 cut(s) | 1948 |
| Cac8I | GCNNGC | 6 cut(s) | 282, 413, 739, 810, 1077, 1247 |
| CfoI | GCGC | 5 cut(s) | 330, 382, 743, 751, 1668 |
| Cfr10I | RCCGGY | 2 cut(s) | 449, 1329 |
| ClaI | ATCGAT | 1 cut(s) | 1620 |
| CpoI | CGGWCCG | 1 cut(s) | 1925 |
| CseI | GACGC | 2 cut(s) | 935, 1826 |
| CsiI | ACCWGGT | 1 cut(s) | 1022 |
| Csp6I | GTAC | 4 cut(s) | 461, 488, 1333, 1562 |
| CspAI | ACCGGT | 1 cut(s) | 1329 |
| CspI | CGGWCCG | 1 cut(s) | 1925 |
| CviAII | CATG | 8 cut(s) | 52, 346, 464, 809, 862, 1157, 1162, 1181 |
| CviQI | GTAC | 4 cut(s) | 461, 488, 1333, 1562 |
| DdeI | CTNAG | 5 cut(s) | 222, 228, 606, 1725, 1914 |
| DinI | GGCGCC | 1 cut(s) | 742 |
| DpnI | GATC | 8 cut(s) | 200, 803, 1056, 1260, 1583, 1814, 1850, 1957 |
| DpnII | GATC | 8 cut(s) | 198, 801, 1054, 1258, 1581, 1812, 1848, 1955 |
| DraIII | CACNNNGTG | 1 cut(s) | 1026 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 595, 875 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1864 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 595, 875 |
| EaeI | YGGCCR | 1 cut(s) | 342 |
| Eam1104I | CTCTTC | 1 cut(s) | 1208 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1864 |
| EarI | CTCTTC | 1 cut(s) | 1208 |
| EciI | GGCGGA | 5 cut(s) | 89, 575, 806, 1010, 1917 |
| Eco130I | CCWWGG | 6 cut(s) | 35, 626, 1156, 1272, 1599, 1689 |
| Eco147I | AGGCCT | 1 cut(s) | 1358 |
| Eco24I | GRGCYC | 1 cut(s) | 1752 |
| Eco47I | GGWCC | 8 cut(s) | 259, 521, 597, 662, 785, 1719, 1925, 1937 |
| Eco47III | AGCGCT | 1 cut(s) | 1667 |
| Eco57I | CTGAAG | 2 cut(s) | 392, 1121 |
| Eco72I | CACGTG | 1 cut(s) | 1676 |
| Eco91I | GGTNACC | 1 cut(s) | 1277 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 986 |
| EcoO109I | RGGNCCY | 2 cut(s) | 259, 662 |
| EcoO65I | GGTNACC | 1 cut(s) | 1277 |
| EcoRI | GAATTC | 1 cut(s) | 1193 |
| EcoRII | CCWGG | 5 cut(s) | 183, 781, 797, 1022, 1290 |
| EcoT14I | CCWWGG | 6 cut(s) | 35, 626, 1156, 1272, 1599, 1689 |
| EcoT38I | GRGCYC | 1 cut(s) | 1752 |
| EgeI | GGCGCC | 1 cut(s) | 742 |
| EheI | GGCGCC | 1 cut(s) | 742 |
| ErhI | CCWWGG | 6 cut(s) | 35, 626, 1156, 1272, 1599, 1689 |
| Esp3I | CGTCTC | 1 cut(s) | 1530 |
| FaeI | CATG | 8 cut(s) | 55, 349, 467, 812, 865, 1160, 1165, 1184 |
| FaqI | GGGAC | 4 cut(s) | 534, 608, 675, 1107 |
| FatI | CATG | 8 cut(s) | 51, 345, 463, 808, 861, 1156, 1161, 1180 |
| FauI | CCCGC | 2 cut(s) | 81, 210 |
| FauNDI | CATATG | 1 cut(s) | 882 |
| FbaI | TGATCA | 2 cut(s) | 1258, 1581 |
| FblI | GTMKAC | 1 cut(s) | 1857 |
| FokI | GGATG | 8 cut(s) | 174, 638, 712, 800, 1027, 1122, 1163, 1309 |
| FriOI | GRGCYC | 1 cut(s) | 1752 |
| FspBI | CTAG | 4 cut(s) | 234, 287, 429, 627 |
| GlaI | GCGC | 5 cut(s) | 329, 381, 742, 750, 1667 |
| GsaI | CCCAGC | 4 cut(s) | 44, 174, 404, 749 |
| GsuI | CTGGAG | 1 cut(s) | 713 |
| HaeII | RGCGCY | 4 cut(s) | 383, 744, 752, 1669 |
| HaeIII | GGCC | 9 cut(s) | 27, 45, 188, 344, 731, 1108, 1358, 1605, 1902 |
| HapII | CCGG | 8 cut(s) | 28, 196, 251, 450, 727, 1330, 1935, 1968 |
| HgaI | GACGC | 2 cut(s) | 935, 1826 |
| HhaI | GCGC | 5 cut(s) | 330, 382, 743, 751, 1668 |
| Hin1I | GRCGYC | 3 cut(s) | 741, 927, 1818 |
| Hin1II | CATG | 8 cut(s) | 55, 349, 467, 812, 865, 1160, 1165, 1184 |
| Hin6I | GCGC | 5 cut(s) | 328, 380, 741, 749, 1666 |
| HinP1I | GCGC | 5 cut(s) | 328, 380, 741, 749, 1666 |
| HincII | GTYRAC | 1 cut(s) | 1858 |
| HindII | GTYRAC | 1 cut(s) | 1858 |
| HinfI | GANTC | 6 cut(s) | 307, 563, 1095, 1595, 1865, 1910 |
| HpaII | CCGG | 8 cut(s) | 28, 196, 251, 450, 727, 1330, 1935, 1968 |
| HphI | GGTGA | 8 cut(s) | 91, 155, 256, 439, 895, 1012, 1112, 1630 |
| Hpy166II | GTNNAC | 4 cut(s) | 1344, 1564, 1722, 1858 |
| Hpy188I | TCNGA | 2 cut(s) | 1207, 1917 |
| Hpy8I | GTNNAC | 4 cut(s) | 1344, 1564, 1722, 1858 |
| Hpy99I | CGWCG | 1 cut(s) | 1820 |
| HpyAV | CCTTC | 8 cut(s) | 143, 370, 652, 901, 1003, 1096, 1768, 1839 |
| HpyCH4III | ACNGT | 2 cut(s) | 1304, 1348 |
| HpyCH4IV | ACGT | 2 cut(s) | 1377, 1675 |
| HpyCH4V | TGCA | 9 cut(s) | 256, 703, 808, 861, 1184, 1386, 1461, 1500, 1701 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 85, 94, 121, 162, 171, 401, 1242, 1707 |
| HpyF3I | CTNAG | 5 cut(s) | 222, 228, 606, 1725, 1914 |
| HpySE526I | ACGT | 2 cut(s) | 1377, 1675 |
| Hsp92I | GRCGYC | 3 cut(s) | 741, 927, 1818 |
| Hsp92II | CATG | 8 cut(s) | 55, 349, 467, 812, 865, 1160, 1165, 1184 |
| HspAI | GCGC | 5 cut(s) | 328, 380, 741, 749, 1666 |
| KasI | GGCGCC | 1 cut(s) | 740 |
| KflI | GGGWCCC | 1 cut(s) | 662 |
| KpnI | GGTACC | 1 cut(s) | 491 |
| Ksp22I | TGATCA | 2 cut(s) | 1258, 1581 |
| Kzo9I | GATC | 8 cut(s) | 198, 801, 1054, 1258, 1581, 1812, 1848, 1955 |
| Lsp1109I | GCAGC | 5 cut(s) | 94, 252, 845, 1245, 1261 |
| LweI | GCATC | 4 cut(s) | 152, 660, 690, 1617 |
| MabI | ACCWGGT | 1 cut(s) | 1022 |
| MaeI | CTAG | 4 cut(s) | 234, 287, 429, 627 |
| MaeII | ACGT | 2 cut(s) | 1377, 1675 |
| MaeIII | GTNAC | 6 cut(s) | 191, 564, 846, 901, 1018, 1277 |
| MalI | GATC | 8 cut(s) | 200, 803, 1056, 1260, 1583, 1814, 1850, 1957 |
| MbiI | CCGCTC | 5 cut(s) | 58, 79, 585, 697, 1284 |
| MboI | GATC | 8 cut(s) | 198, 801, 1054, 1258, 1581, 1812, 1848, 1955 |
| MboII | GAAGA | 5 cut(s) | 28, 385, 398, 1225, 1876 |
| MfeI | CAATTG | 2 cut(s) | 1495, 1518 |
| MflI | RGATCY | 3 cut(s) | 198, 801, 1848 |
| MhlI | GDGCHC | 4 cut(s) | 428, 1633, 1752, 1779 |
| MlsI | TGGCCA | 1 cut(s) | 344 |
| MluCI | AATT | 8 cut(s) | 359, 707, 1126, 1193, 1395, 1495, 1518, 1553 |
| MluNI | TGGCCA | 1 cut(s) | 344 |
| Mly113I | GGCGCC | 1 cut(s) | 741 |
| MlyI | GAGTC | 4 cut(s) | 572, 1089, 1604, 1874 |
| MmeI | TCCRAC | 3 cut(s) | 98, 139, 528 |
| Mox20I | TGGCCA | 1 cut(s) | 344 |
| MscI | TGGCCA | 1 cut(s) | 344 |
| MslI | CAYNNNNRTG | 6 cut(s) | 269, 350, 479, 1045, 1140, 1643 |
| Msp20I | TGGCCA | 1 cut(s) | 344 |
| MspA1I | CMGCKG | 4 cut(s) | 85, 221, 1479, 1871 |
| MspI | CCGG | 8 cut(s) | 28, 196, 251, 450, 727, 1330, 1935, 1968 |
| MspR9I | CCNGG | 6 cut(s) | 185, 728, 783, 799, 1024, 1292 |
| MunI | CAATTG | 2 cut(s) | 1495, 1518 |
| MvaI | CCWGG | 5 cut(s) | 185, 783, 799, 1024, 1292 |
| MvnI | CGCG | 2 cut(s) | 421, 1053 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 85, 94, 121, 162, 171, 401, 1242, 1707 |
| NarI | GGCGCC | 1 cut(s) | 741 |
| NciI | CCSGG | 1 cut(s) | 728 |
| NcoI | CCATGG | 1 cut(s) | 1156 |
| NdeI | CATATG | 1 cut(s) | 882 |
| NdeII | GATC | 8 cut(s) | 198, 801, 1054, 1258, 1581, 1812, 1848, 1955 |
| NlaIII | CATG | 8 cut(s) | 55, 349, 467, 812, 865, 1160, 1165, 1184 |
| NmeAIII | GCCGAG | 2 cut(s) | 71, 757 |
| NmuCI | GTSAC | 4 cut(s) | 564, 846, 901, 1018 |
| NruI | TCGCGA | 1 cut(s) | 1053 |
| NspI | RCATGY | 2 cut(s) | 55, 812 |
| PaeI | GCATGC | 1 cut(s) | 812 |
| PceI | AGGCCT | 1 cut(s) | 1358 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1824 |
| PfeI | GAWTC | 2 cut(s) | 307, 1910 |
| PinAI | ACCGGT | 1 cut(s) | 1329 |
| PleI | GAGTC | 4 cut(s) | 571, 1089, 1603, 1873 |
| PluTI | GGCGCC | 1 cut(s) | 744 |
| PmaCI | CACGTG | 1 cut(s) | 1676 |
| PmlI | CACGTG | 1 cut(s) | 1676 |
| PpsI | GAGTC | 4 cut(s) | 571, 1089, 1603, 1873 |
| Ppu21I | YACGTR | 1 cut(s) | 1676 |
| PpuMI | RGGWCCY | 2 cut(s) | 259, 662 |
| Psp5II | RGGWCCY | 2 cut(s) | 259, 662 |
| Psp6I | CCWGG | 5 cut(s) | 183, 781, 797, 1022, 1290 |
| PspCI | CACGTG | 1 cut(s) | 1676 |
| PspEI | GGTNACC | 1 cut(s) | 1277 |
| PspFI | CCCAGC | 4 cut(s) | 40, 170, 400, 745 |
| PspGI | CCWGG | 5 cut(s) | 183, 781, 797, 1022, 1290 |
| PspPPI | RGGWCCY | 2 cut(s) | 259, 662 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1325, 1357 |
| PsuI | RGATCY | 3 cut(s) | 198, 801, 1848 |
| PvuII | CAGCTG | 1 cut(s) | 221 |
| RruI | TCGCGA | 1 cut(s) | 1053 |
| RsaI | GTAC | 4 cut(s) | 462, 489, 1334, 1563 |
| RsaNI | GTAC | 4 cut(s) | 461, 488, 1333, 1562 |
| RseI | CAYNNNNRTG | 6 cut(s) | 269, 350, 479, 1045, 1140, 1643 |
| Rsr2I | CGGWCCG | 1 cut(s) | 1925 |
| RsrII | CGGWCCG | 1 cut(s) | 1925 |
| SalI | GTCGAC | 1 cut(s) | 1856 |
| Sau3AI | GATC | 8 cut(s) | 198, 801, 1054, 1258, 1581, 1812, 1848, 1955 |
| SchI | GAGTC | 4 cut(s) | 572, 1089, 1604, 1874 |
| ScrFI | CCNGG | 6 cut(s) | 185, 728, 783, 799, 1024, 1292 |
| SduI | GDGCHC | 4 cut(s) | 428, 1633, 1752, 1779 |
| SexAI | ACCWGGT | 1 cut(s) | 1022 |
| SfaNI | GCATC | 4 cut(s) | 152, 660, 690, 1617 |
| SfcI | CTRYAG | 1 cut(s) | 216 |
| SfoI | GGCGCC | 1 cut(s) | 742 |
| SgrAI | CRCCGGYG | 1 cut(s) | 449 |
| SinI | GGWCC | 8 cut(s) | 259, 521, 597, 662, 785, 1719, 1925, 1937 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 269, 350, 479, 1045, 1140, 1643 |
| SmlI | CTYRAG | 2 cut(s) | 983, 1352 |
| SmoI | CTYRAG | 2 cut(s) | 983, 1352 |
| SphI | GCATGC | 1 cut(s) | 812 |
| Sse9I | AATT | 8 cut(s) | 359, 707, 1126, 1193, 1395, 1495, 1518, 1553 |
| SseBI | AGGCCT | 1 cut(s) | 1358 |
| SspDI | GGCGCC | 1 cut(s) | 740 |
| SspI | AATATT | 1 cut(s) | 1705 |
| SspMI | CTAG | 4 cut(s) | 234, 287, 429, 627 |
| StuI | AGGCCT | 1 cut(s) | 1358 |
| StyD4I | CCNGG | 6 cut(s) | 183, 726, 781, 797, 1022, 1290 |
| StyI | CCWWGG | 6 cut(s) | 35, 626, 1156, 1272, 1599, 1689 |
| TaaI | ACNGT | 2 cut(s) | 1304, 1348 |
| TaiI | ACGT | 2 cut(s) | 1380, 1678 |
| TaqI | TCGA | 6 cut(s) | 587, 1059, 1620, 1815, 1827, 1857 |
| TasI | AATT | 8 cut(s) | 359, 707, 1126, 1193, 1395, 1495, 1518, 1553 |
| TfiI | GAWTC | 2 cut(s) | 307, 1910 |
| TscAI | CASTG | 6 cut(s) | 799, 1052, 1344, 1351, 1388, 1845 |
| TseFI | GTSAC | 4 cut(s) | 564, 846, 901, 1018 |
| TseI | GCWGC | 5 cut(s) | 82, 240, 858, 1233, 1249 |
| Tsp45I | GTSAC | 4 cut(s) | 564, 846, 901, 1018 |
| TspDTI | ATGAA | 2 cut(s) | 17, 1186 |
| TspGWI | ACGGA | 2 cut(s) | 843, 1794 |
| TspRI | CASTG | 6 cut(s) | 799, 1052, 1344, 1351, 1388, 1845 |
| VpaK11BI | GGWCC | 8 cut(s) | 259, 521, 597, 662, 785, 1719, 1925, 1937 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 986 |
| XapI | RAATTY | 3 cut(s) | 359, 1193, 1395 |
| XceI | RCATGY | 2 cut(s) | 55, 812 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1030 |
| XmaJI | CCTAGG | 1 cut(s) | 626 |
| XmiI | GTMKAC | 1 cut(s) | 1857 |
| XspI | CTAG | 4 cut(s) | 234, 287, 429, 627 |