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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 73 |
| Acc36I | ACCTGC | 1 cut(s) | 73 |
| AccB1I | GGYRCC | 1 cut(s) | 211 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 256 |
| AccII | CGCG | 1 cut(s) | 319 |
| AciI | CCGC | 4 cut(s) | 17, 317, 362, 404 |
| AcoI | YGGCCR | 1 cut(s) | 87 |
| AcuI | CTGAAG | 2 cut(s) | 372, 398 |
| AfaI | GTAC | 4 cut(s) | 172, 207, 222, 234 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 28, 82, 194, 256, 403 |
| AjnI | CCWGG | 2 cut(s) | 338, 371 |
| AluBI | AGCT | 4 cut(s) | 7, 94, 253, 382 |
| AluI | AGCT | 4 cut(s) | 7, 94, 253, 382 |
| Alw21I | GWGCWC | 1 cut(s) | 45 |
| AoxI | GGCC | 3 cut(s) | 72, 87, 407 |
| ApaI | GGGCCC | 1 cut(s) | 411 |
| AspLEI | GCGC | 3 cut(s) | 57, 151, 321 |
| AspS9I | GGNCC | 5 cut(s) | 19, 73, 266, 407, 408 |
| AsuHPI | GGTGA | 1 cut(s) | 270 |
| AvaII | GGWCC | 2 cut(s) | 19, 266 |
| BaeGI | GKGCMC | 1 cut(s) | 411 |
| BalI | TGGCCA | 1 cut(s) | 89 |
| BanI | GGYRCC | 1 cut(s) | 211 |
| BanII | GRGCYC | 1 cut(s) | 411 |
| BauI | CACGAG | 1 cut(s) | 44 |
| Bbv12I | GWGCWC | 1 cut(s) | 45 |
| BciT130I | CCWGG | 2 cut(s) | 340, 373 |
| BfaI | CTAG | 2 cut(s) | 345, 414 |
| BfuAI | ACCTGC | 1 cut(s) | 73 |
| Bme1390I | CCNGG | 2 cut(s) | 340, 373 |
| Bme18I | GGWCC | 2 cut(s) | 19, 266 |
| BmgT120I | GGNCC | 5 cut(s) | 19, 73, 266, 407, 408 |
| BmiI | GGNNCC | 4 cut(s) | 213, 267, 408, 409 |
| BmrFI | CCNGG | 2 cut(s) | 340, 373 |
| BmsI | GCATC | 1 cut(s) | 216 |
| BsaJI | CCNNGG | 2 cut(s) | 339, 371 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 28, 82, 194, 256, 403 |
| Bse1I | ACTGG | 1 cut(s) | 18 |
| BseBI | CCWGG | 2 cut(s) | 340, 373 |
| BseDI | CCNNGG | 2 cut(s) | 339, 371 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 28, 82, 194, 256, 403 |
| BseNI | ACTGG | 1 cut(s) | 18 |
| BseSI | GKGCMC | 1 cut(s) | 411 |
| BseYI | CCCAGC | 2 cut(s) | 145, 249 |
| Bsh1236I | CGCG | 1 cut(s) | 319 |
| BshFI | GGCC | 3 cut(s) | 74, 89, 409 |
| BshNI | GGYRCC | 1 cut(s) | 211 |
| BsiHKAI | GWGCWC | 1 cut(s) | 45 |
| BsiSI | CCGG | 1 cut(s) | 195 |
| BslFI | GGGAC | 1 cut(s) | 232 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 28, 82, 194, 256, 403 |
| BsmFI | GGGAC | 1 cut(s) | 232 |
| BsnI | GGCC | 3 cut(s) | 74, 89, 409 |
| Bsp120I | GGGCCC | 1 cut(s) | 407 |
| Bsp1286I | GDGCHC | 2 cut(s) | 45, 411 |
| BspACI | CCGC | 4 cut(s) | 17, 317, 362, 404 |
| BspANI | GGCC | 3 cut(s) | 74, 89, 409 |
| BspFNI | CGCG | 1 cut(s) | 319 |
| BspLI | GGNNCC | 4 cut(s) | 213, 267, 408, 409 |
| BspMI | ACCTGC | 1 cut(s) | 73 |
| BspT107I | GGYRCC | 1 cut(s) | 211 |
| BsrI | ACTGG | 1 cut(s) | 18 |
| BssECI | CCNNGG | 2 cut(s) | 339, 371 |
| BssSI | CACGAG | 1 cut(s) | 44 |
| Bst2BI | CACGAG | 1 cut(s) | 44 |
| Bst2UI | CCWGG | 2 cut(s) | 340, 373 |
| BstC8I | GCNNGC | 3 cut(s) | 9, 366, 384 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 26 |
| BstFNI | CGCG | 1 cut(s) | 319 |
| BstHHI | GCGC | 3 cut(s) | 57, 151, 321 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 131, 146, 157, 341, 388 |
| BstNI | CCWGG | 2 cut(s) | 340, 373 |
| BstSCI | CCNGG | 2 cut(s) | 338, 371 |
| BstSLI | GKGCMC | 1 cut(s) | 411 |
| BstUI | CGCG | 1 cut(s) | 319 |
| BsuRI | GGCC | 3 cut(s) | 74, 89, 409 |
| BtsIMutI | CAGTG | 1 cut(s) | 11 |
| BveI | ACCTGC | 1 cut(s) | 73 |
| Cac8I | GCNNGC | 3 cut(s) | 9, 366, 384 |
| CfoI | GCGC | 3 cut(s) | 57, 151, 321 |
| Cfr13I | GGNCC | 5 cut(s) | 19, 73, 266, 407, 408 |
| CseI | GACGC | 1 cut(s) | 428 |
| Csp6I | GTAC | 4 cut(s) | 171, 206, 221, 233 |
| CviQI | GTAC | 4 cut(s) | 171, 206, 221, 233 |
| EaeI | YGGCCR | 1 cut(s) | 87 |
| EciI | GGCGGA | 2 cut(s) | 32, 351 |
| Eco24I | GRGCYC | 1 cut(s) | 411 |
| Eco47I | GGWCC | 2 cut(s) | 19, 266 |
| Eco57I | CTGAAG | 2 cut(s) | 372, 398 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 26 |
| EcoO109I | RGGNCCY | 1 cut(s) | 407 |
| EcoRII | CCWGG | 2 cut(s) | 338, 371 |
| EcoT38I | GRGCYC | 1 cut(s) | 411 |
| FaiI | YATR | 3 cut(s) | 180, 431, 439 |
| FaqI | GGGAC | 1 cut(s) | 232 |
| FauI | CCCGC | 1 cut(s) | 397 |
| FriOI | GRGCYC | 1 cut(s) | 411 |
| FspBI | CTAG | 2 cut(s) | 345, 414 |
| GlaI | GCGC | 3 cut(s) | 56, 150, 320 |
| GsaI | CCCAGC | 2 cut(s) | 149, 253 |
| HaeIII | GGCC | 3 cut(s) | 74, 89, 409 |
| HapII | CCGG | 1 cut(s) | 195 |
| HgaI | GACGC | 1 cut(s) | 428 |
| HhaI | GCGC | 3 cut(s) | 57, 151, 321 |
| Hin6I | GCGC | 3 cut(s) | 55, 149, 319 |
| HinP1I | GCGC | 3 cut(s) | 55, 149, 319 |
| HincII | GTYRAC | 1 cut(s) | 185 |
| HindII | GTYRAC | 1 cut(s) | 185 |
| HinfI | GANTC | 1 cut(s) | 308 |
| HpaII | CCGG | 1 cut(s) | 195 |
| HphI | GGTGA | 1 cut(s) | 270 |
| Hpy166II | GTNNAC | 2 cut(s) | 185, 269 |
| Hpy8I | GTNNAC | 2 cut(s) | 185, 269 |
| Hpy99I | CGWCG | 1 cut(s) | 422 |
| HpyAV | CCTTC | 3 cut(s) | 58, 115, 347 |
| HpyCH4V | TGCA | 2 cut(s) | 229, 335 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 131, 146, 157, 341, 388 |
| HspAI | GCGC | 3 cut(s) | 55, 149, 319 |
| LmnI | GCTCC | 3 cut(s) | 4, 40, 373 |
| LweI | GCATC | 1 cut(s) | 216 |
| MaeI | CTAG | 2 cut(s) | 345, 414 |
| MboII | GAAGA | 1 cut(s) | 210 |
| MhlI | GDGCHC | 2 cut(s) | 45, 411 |
| MlsI | TGGCCA | 1 cut(s) | 89 |
| MluNI | TGGCCA | 1 cut(s) | 89 |
| MnlI | CCTC | 4 cut(s) | 32, 64, 286, 407 |
| Mox20I | TGGCCA | 1 cut(s) | 89 |
| MscI | TGGCCA | 1 cut(s) | 89 |
| MseI | TTAA | 1 cut(s) | 287 |
| Msp20I | TGGCCA | 1 cut(s) | 89 |
| MspI | CCGG | 1 cut(s) | 195 |
| MspR9I | CCNGG | 2 cut(s) | 340, 373 |
| MvaI | CCWGG | 2 cut(s) | 340, 373 |
| MvnI | CGCG | 1 cut(s) | 319 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 131, 146, 157, 341, 388 |
| NlaIV | GGNNCC | 4 cut(s) | 213, 267, 408, 409 |
| PaqCI | CACCTGC | 1 cut(s) | 73 |
| PfeI | GAWTC | 1 cut(s) | 308 |
| PflFI | GACNNNGTC | 1 cut(s) | 180 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 256 |
| Psp6I | CCWGG | 2 cut(s) | 338, 371 |
| PspFI | CCCAGC | 2 cut(s) | 145, 249 |
| PspGI | CCWGG | 2 cut(s) | 338, 371 |
| PspN4I | GGNNCC | 4 cut(s) | 213, 267, 408, 409 |
| PspOMI | GGGCCC | 1 cut(s) | 407 |
| PspPI | GGNCC | 5 cut(s) | 19, 73, 266, 407, 408 |
| PsyI | GACNNNGTC | 1 cut(s) | 180 |
| RsaI | GTAC | 4 cut(s) | 172, 207, 222, 234 |
| RsaNI | GTAC | 4 cut(s) | 171, 206, 221, 233 |
| SaqAI | TTAA | 1 cut(s) | 287 |
| Sau96I | GGNCC | 5 cut(s) | 19, 73, 266, 407, 408 |
| ScrFI | CCNGG | 2 cut(s) | 340, 373 |
| SduI | GDGCHC | 2 cut(s) | 45, 411 |
| SetI | ASST | 8 cut(s) | 9, 24, 87, 96, 107, 255, 384, 399 |
| SfaNI | GCATC | 1 cut(s) | 216 |
| SinI | GGWCC | 2 cut(s) | 19, 266 |
| SsiI | CCGC | 4 cut(s) | 17, 317, 362, 404 |
| SspMI | CTAG | 2 cut(s) | 345, 414 |
| StyD4I | CCNGG | 2 cut(s) | 338, 371 |
| TaqI | TCGA | 1 cut(s) | 108 |
| TfiI | GAWTC | 1 cut(s) | 308 |
| Tru1I | TTAA | 1 cut(s) | 287 |
| Tru9I | TTAA | 1 cut(s) | 287 |
| TscAI | CASTG | 1 cut(s) | 18 |
| TspRI | CASTG | 1 cut(s) | 18 |
| Tth111I | GACNNNGTC | 1 cut(s) | 180 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 256 |
| VpaK11BI | GGWCC | 2 cut(s) | 19, 266 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 26 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 379 |
| XspI | CTAG | 2 cut(s) | 345, 414 |