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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 669 |
| Acc36I | ACCTGC | 1 cut(s) | 554 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 608 |
| AccBSI | CCGCTC | 1 cut(s) | 502 |
| AccI | GTMKAC | 1 cut(s) | 356 |
| AccII | CGCG | 4 cut(s) | 72, 264, 338, 717 |
| AclI | AACGTT | 1 cut(s) | 876 |
| AclWI | GGATC | 7 cut(s) | 123, 166, 199, 525, 635, 713, 783 |
| AcoI | YGGCCR | 4 cut(s) | 106, 259, 304, 311 |
| AcuI | CTGAAG | 1 cut(s) | 46 |
| AcyI | GRCGYC | 3 cut(s) | 658, 666, 847 |
| AdeI | CACNNNGTG | 1 cut(s) | 856 |
| AfaI | GTAC | 5 cut(s) | 174, 371, 394, 406, 541 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 75, 254, 366, 517, 544, 608, 736 |
| AflIII | ACRYGT | 1 cut(s) | 273 |
| AjnI | CCWGG | 7 cut(s) | 131, 154, 380, 419, 510, 571, 643 |
| AluBI | AGCT | 2 cut(s) | 142, 605 |
| AluI | AGCT | 2 cut(s) | 142, 605 |
| Alw21I | GWGCWC | 1 cut(s) | 92 |
| Alw26I | GTCTC | 1 cut(s) | 645 |
| AlwI | GGATC | 7 cut(s) | 123, 166, 199, 525, 635, 713, 783 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 142 |
| ApeKI | GCWGC | 4 cut(s) | 136, 139, 142, 340 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 484 |
| AspA2I | CCTAGG | 1 cut(s) | 543 |
| AspLEI | GCGC | 5 cut(s) | 229, 266, 340, 366, 550 |
| AspS9I | GGNCC | 3 cut(s) | 384, 438, 702 |
| AsuC2I | CCSGG | 4 cut(s) | 105, 115, 318, 700 |
| AsuHPI | GGTGA | 4 cut(s) | 132, 158, 540, 815 |
| AvaII | GGWCC | 1 cut(s) | 438 |
| AvrII | CCTAGG | 1 cut(s) | 543 |
| Bbv12I | GWGCWC | 1 cut(s) | 92 |
| BbvI | GCAGC | 4 cut(s) | 129, 148, 151, 352 |
| BccI | CCATC | 1 cut(s) | 57 |
| BceAI | ACGGC | 1 cut(s) | 717 |
| BciT130I | CCWGG | 7 cut(s) | 133, 156, 382, 421, 512, 573, 645 |
| BcnI | CCSGG | 4 cut(s) | 105, 115, 318, 700 |
| BcoDI | GTCTC | 1 cut(s) | 645 |
| BfaI | CTAG | 1 cut(s) | 544 |
| BfmI | CTRYAG | 1 cut(s) | 723 |
| BfoI | RGCGCY | 1 cut(s) | 367 |
| BfuAI | ACCTGC | 1 cut(s) | 554 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 151, 560 |
| BisI | GCNGC | 9 cut(s) | 137, 140, 143, 262, 304, 314, 341, 715, 730 |
| BlnI | CCTAGG | 1 cut(s) | 543 |
| BlsI | GCNGC | 9 cut(s) | 138, 141, 144, 263, 305, 315, 342, 716, 731 |
| Bme18I | GGWCC | 1 cut(s) | 438 |
| BmgT120I | GGNCC | 3 cut(s) | 384, 438, 702 |
| BmiI | GGNNCC | 3 cut(s) | 439, 678, 704 |
| BmsI | GCATC | 3 cut(s) | 367, 581, 607 |
| BoxI | GACNNNNGTC | 1 cut(s) | 657 |
| BpmI | CTGGAG | 1 cut(s) | 648 |
| BpuMI | CCSGG | 4 cut(s) | 105, 115, 318, 700 |
| BsaHI | GRCGYC | 3 cut(s) | 658, 666, 847 |
| BsaJI | CCNNGG | 6 cut(s) | 103, 113, 381, 420, 511, 543 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 621, 651 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 75, 254, 366, 517, 544, 608, 736 |
| Bse118I | RCCGGY | 4 cut(s) | 309, 366, 386, 585 |
| Bse1I | ACTGG | 2 cut(s) | 190, 631 |
| BseBI | CCWGG | 7 cut(s) | 133, 156, 382, 421, 512, 573, 645 |
| BseDI | CCNNGG | 6 cut(s) | 103, 113, 381, 420, 511, 543 |
| BseGI | GGATG | 2 cut(s) | 49, 622 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 75, 254, 366, 517, 544, 608, 736 |
| BseMII | CTCAG | 1 cut(s) | 514 |
| BseNI | ACTGG | 2 cut(s) | 190, 631 |
| BseX3I | CGGCCG | 1 cut(s) | 311 |
| BseXI | GCAGC | 4 cut(s) | 129, 148, 151, 352 |
| BseYI | CCCAGC | 2 cut(s) | 360, 601 |
| Bsh1236I | CGCG | 4 cut(s) | 72, 264, 338, 717 |
| Bsh1285I | CGRYCG | 3 cut(s) | 76, 314, 391 |
| BsiEI | CGRYCG | 3 cut(s) | 76, 314, 391 |
| BsiHKAI | GWGCWC | 1 cut(s) | 92 |
| BsiSI | CCGG | 8 cut(s) | 105, 115, 310, 318, 367, 387, 586, 700 |
| BslFI | GGGAC | 2 cut(s) | 451, 645 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 75, 254, 366, 517, 544, 608, 736 |
| BsmAI | GTCTC | 1 cut(s) | 645 |
| BsmBI | CGTCTC | 1 cut(s) | 645 |
| BsmFI | GGGAC | 2 cut(s) | 451, 645 |
| BsmI | GAATGC | 1 cut(s) | 512 |
| Bsp1286I | GDGCHC | 1 cut(s) | 92 |
| Bsp143I | GATC | 8 cut(s) | 73, 128, 158, 191, 530, 640, 718, 788 |
| BspCNI | CTCAG | 1 cut(s) | 515 |
| BspFNI | CGCG | 4 cut(s) | 72, 264, 338, 717 |
| BspHI | TCATGA | 1 cut(s) | 268 |
| BspLI | GGNNCC | 3 cut(s) | 439, 678, 704 |
| BspMAI | CTGCAG | 1 cut(s) | 727 |
| BspMI | ACCTGC | 1 cut(s) | 554 |
| BspPI | GGATC | 7 cut(s) | 123, 166, 199, 525, 635, 713, 783 |
| BspQI | GCTCTTC | 1 cut(s) | 22 |
| BsrBI | CCGCTC | 1 cut(s) | 502 |
| BsrFI | RCCGGY | 4 cut(s) | 309, 366, 386, 585 |
| BsrI | ACTGG | 2 cut(s) | 190, 631 |
| BssAI | RCCGGY | 4 cut(s) | 309, 366, 386, 585 |
| BssECI | CCNNGG | 6 cut(s) | 103, 113, 381, 420, 511, 543 |
| BssMI | GATC | 8 cut(s) | 73, 128, 158, 191, 530, 640, 718, 788 |
| BssNI | GRCGYC | 3 cut(s) | 658, 666, 847 |
| BssT1I | CCWWGG | 1 cut(s) | 543 |
| Bst2UI | CCWGG | 7 cut(s) | 133, 156, 382, 421, 512, 573, 645 |
| Bst6I | CTCTTC | 1 cut(s) | 22 |
| BstACI | GRCGYC | 3 cut(s) | 658, 666, 847 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 142 |
| BstC8I | GCNNGC | 4 cut(s) | 330, 552, 587, 727 |
| BstDEI | CTNAG | 1 cut(s) | 523 |
| BstEII | GGTNACC | 1 cut(s) | 821 |
| BstF5I | GGATG | 2 cut(s) | 49, 622 |
| BstFNI | CGCG | 4 cut(s) | 72, 264, 338, 717 |
| BstH2I | RGCGCY | 1 cut(s) | 367 |
| BstHHI | GCGC | 5 cut(s) | 229, 266, 340, 366, 550 |
| BstKTI | GATC | 8 cut(s) | 76, 131, 161, 194, 533, 643, 721, 791 |
| BstMAI | GTCTC | 1 cut(s) | 645 |
| BstMBI | GATC | 8 cut(s) | 73, 128, 158, 191, 530, 640, 718, 788 |
| BstMCI | CGRYCG | 3 cut(s) | 76, 314, 391 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 142, 151, 407, 560, 582, 591 |
| BstNI | CCWGG | 7 cut(s) | 133, 156, 382, 421, 512, 573, 645 |
| BstNSI | RCATGY | 1 cut(s) | 405 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 657 |
| BstPI | GGTNACC | 1 cut(s) | 821 |
| BstSFI | CTRYAG | 1 cut(s) | 723 |
| BstUI | CGCG | 4 cut(s) | 72, 264, 338, 717 |
| BstV1I | GCAGC | 4 cut(s) | 129, 148, 151, 352 |
| BstX2I | RGATCY | 3 cut(s) | 128, 158, 191 |
| BstYI | RGATCY | 3 cut(s) | 128, 158, 191 |
| BstZI | CGGCCG | 1 cut(s) | 311 |
| BtgZI | GCGATG | 1 cut(s) | 604 |
| BtsCI | GGATG | 2 cut(s) | 49, 622 |
| BtsIMutI | CAGTG | 1 cut(s) | 183 |
| BveI | ACCTGC | 1 cut(s) | 554 |
| Cac8I | GCNNGC | 4 cut(s) | 330, 552, 587, 727 |
| CaiI | CAGNNNCTG | 1 cut(s) | 142 |
| CciI | TCATGA | 1 cut(s) | 268 |
| CfoI | GCGC | 5 cut(s) | 229, 266, 340, 366, 550 |
| Cfr10I | RCCGGY | 4 cut(s) | 309, 366, 386, 585 |
| Cfr13I | GGNCC | 3 cut(s) | 384, 438, 702 |
| CseI | GACGC | 2 cut(s) | 647, 855 |
| Csp6I | GTAC | 5 cut(s) | 173, 370, 393, 405, 540 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 406, 441 |
| CviAII | CATG | 4 cut(s) | 170, 269, 402, 803 |
| CviQI | GTAC | 5 cut(s) | 173, 370, 393, 405, 540 |
| DdeI | CTNAG | 1 cut(s) | 523 |
| DpnI | GATC | 8 cut(s) | 75, 130, 160, 193, 532, 642, 720, 790 |
| DpnII | GATC | 8 cut(s) | 73, 128, 158, 191, 530, 640, 718, 788 |
| DraIII | CACNNNGTG | 1 cut(s) | 856 |
| EaeI | YGGCCR | 4 cut(s) | 106, 259, 304, 311 |
| EagI | CGGCCG | 1 cut(s) | 311 |
| Eam1104I | CTCTTC | 1 cut(s) | 22 |
| EarI | CTCTTC | 1 cut(s) | 22 |
| EciI | GGCGGA | 8 cut(s) | 204, 237, 492, 523, 572, 663, 701, 793 |
| EclXI | CGGCCG | 1 cut(s) | 311 |
| Eco130I | CCWWGG | 1 cut(s) | 543 |
| Eco147I | AGGCCT | 1 cut(s) | 576 |
| Eco47I | GGWCC | 1 cut(s) | 438 |
| Eco52I | CGGCCG | 1 cut(s) | 311 |
| Eco57I | CTGAAG | 1 cut(s) | 46 |
| Eco91I | GGTNACC | 1 cut(s) | 821 |
| EcoO65I | GGTNACC | 1 cut(s) | 821 |
| EcoRII | CCWGG | 7 cut(s) | 131, 154, 380, 419, 510, 571, 643 |
| EcoT14I | CCWWGG | 1 cut(s) | 543 |
| EcoT22I | ATGCAT | 1 cut(s) | 403 |
| ErhI | CCWWGG | 1 cut(s) | 543 |
| Esp3I | CGTCTC | 1 cut(s) | 645 |
| FaeI | CATG | 4 cut(s) | 173, 272, 405, 806 |
| FaiI | YATR | 9 cut(s) | 171, 199, 270, 403, 450, 746, 748, 775, 804 |
| FaqI | GGGAC | 2 cut(s) | 451, 645 |
| FatI | CATG | 4 cut(s) | 169, 268, 401, 802 |
| FauI | CCCGC | 4 cut(s) | 6, 53, 77, 688 |
| FauNDI | CATATG | 1 cut(s) | 746 |
| FblI | GTMKAC | 1 cut(s) | 356 |
| Fnu4HI | GCNGC | 9 cut(s) | 137, 140, 143, 262, 304, 314, 341, 715, 730 |
| FokI | GGATG | 2 cut(s) | 36, 629 |
| Fsp4HI | GCNGC | 9 cut(s) | 137, 140, 143, 262, 304, 314, 341, 715, 730 |
| FspBI | CTAG | 1 cut(s) | 544 |
| GlaI | GCGC | 5 cut(s) | 228, 265, 339, 365, 549 |
| GluI | GCNGC | 9 cut(s) | 137, 140, 143, 262, 304, 314, 341, 715, 730 |
| GsaI | CCCAGC | 2 cut(s) | 364, 605 |
| GsuI | CTGGAG | 1 cut(s) | 648 |
| HaeII | RGCGCY | 1 cut(s) | 367 |
| HapII | CCGG | 8 cut(s) | 105, 115, 310, 318, 367, 387, 586, 700 |
| HgaI | GACGC | 2 cut(s) | 647, 855 |
| HhaI | GCGC | 5 cut(s) | 229, 266, 340, 366, 550 |
| Hin1I | GRCGYC | 3 cut(s) | 658, 666, 847 |
| Hin1II | CATG | 4 cut(s) | 173, 272, 405, 806 |
| Hin6I | GCGC | 5 cut(s) | 227, 264, 338, 364, 548 |
| HinP1I | GCGC | 5 cut(s) | 227, 264, 338, 364, 548 |
| HincII | GTYRAC | 3 cut(s) | 83, 357, 670 |
| HindII | GTYRAC | 3 cut(s) | 83, 357, 670 |
| HinfI | GANTC | 1 cut(s) | 480 |
| HpaI | GTTAAC | 1 cut(s) | 83 |
| HpaII | CCGG | 8 cut(s) | 105, 115, 310, 318, 367, 387, 586, 700 |
| HphI | GGTGA | 4 cut(s) | 132, 158, 540, 815 |
| Hpy166II | GTNNAC | 5 cut(s) | 83, 357, 670, 688, 800 |
| Hpy188I | TCNGA | 2 cut(s) | 65, 676 |
| Hpy188III | TCNNGA | 4 cut(s) | 269, 430, 662, 694 |
| Hpy8I | GTNNAC | 5 cut(s) | 83, 357, 670, 688, 800 |
| Hpy99I | CGWCG | 2 cut(s) | 668, 849 |
| HpyAV | CCTTC | 2 cut(s) | 230, 812 |
| HpyCH4IV | ACGT | 4 cut(s) | 275, 350, 666, 876 |
| HpyCH4V | TGCA | 5 cut(s) | 401, 594, 620, 725, 750 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 142, 151, 407, 560, 582, 591 |
| HpyF3I | CTNAG | 1 cut(s) | 523 |
| HpySE526I | ACGT | 4 cut(s) | 275, 350, 666, 876 |
| Hsp92I | GRCGYC | 3 cut(s) | 658, 666, 847 |
| Hsp92II | CATG | 4 cut(s) | 173, 272, 405, 806 |
| HspAI | GCGC | 5 cut(s) | 227, 264, 338, 364, 548 |
| KroI | GCCGGC | 1 cut(s) | 585 |
| KroNI | GCCGGC | 1 cut(s) | 587 |
| KspAI | GTTAAC | 1 cut(s) | 83 |
| Kzo9I | GATC | 8 cut(s) | 73, 128, 158, 191, 530, 640, 718, 788 |
| LguI | GCTCTTC | 1 cut(s) | 22 |
| LmnI | GCTCC | 2 cut(s) | 224, 629 |
| Lsp1109I | GCAGC | 4 cut(s) | 129, 148, 151, 352 |
| LweI | GCATC | 3 cut(s) | 367, 581, 607 |
| MaeI | CTAG | 1 cut(s) | 544 |
| MaeII | ACGT | 4 cut(s) | 275, 350, 666, 876 |
| MaeIII | GTNAC | 2 cut(s) | 164, 821 |
| MalI | GATC | 8 cut(s) | 75, 130, 160, 193, 532, 642, 720, 790 |
| MbiI | CCGCTC | 1 cut(s) | 502 |
| MboI | GATC | 8 cut(s) | 73, 128, 158, 191, 530, 640, 718, 788 |
| MboII | GAAGA | 2 cut(s) | 39, 186 |
| MflI | RGATCY | 3 cut(s) | 128, 158, 191 |
| MhlI | GDGCHC | 1 cut(s) | 92 |
| MluCI | AATT | 1 cut(s) | 285 |
| MmeI | TCCRAC | 2 cut(s) | 88, 859 |
| MnlI | CCTC | 7 cut(s) | 71, 116, 212, 275, 518, 628, 881 |
| Mph1103I | ATGCAT | 1 cut(s) | 403 |
| MreI | CGCCGGCG | 1 cut(s) | 585 |
| MroNI | GCCGGC | 1 cut(s) | 585 |
| MroXI | GAANNNNTTC | 1 cut(s) | 484 |
| MseI | TTAA | 3 cut(s) | 82, 288, 814 |
| MspA1I | CMGCKG | 4 cut(s) | 13, 142, 410, 683 |
| MspI | CCGG | 8 cut(s) | 105, 115, 310, 318, 367, 387, 586, 700 |
| Mva1269I | GAATGC | 1 cut(s) | 512 |
| MvaI | CCWGG | 7 cut(s) | 133, 156, 382, 421, 512, 573, 645 |
| MvnI | CGCG | 4 cut(s) | 72, 264, 338, 717 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 142, 151, 407, 560, 582, 591 |
| NaeI | GCCGGC | 1 cut(s) | 587 |
| NciI | CCSGG | 4 cut(s) | 105, 115, 318, 700 |
| NdeI | CATATG | 1 cut(s) | 746 |
| NdeII | GATC | 8 cut(s) | 73, 128, 158, 191, 530, 640, 718, 788 |
| NgoMIV | GCCGGC | 1 cut(s) | 585 |
| NlaIII | CATG | 4 cut(s) | 173, 272, 405, 806 |
| NlaIV | GGNNCC | 3 cut(s) | 439, 678, 704 |
| NmeAIII | GCCGAG | 1 cut(s) | 539 |
| NmuCI | GTSAC | 2 cut(s) | 164, 821 |
| NsiI | ATGCAT | 1 cut(s) | 403 |
| NspI | RCATGY | 1 cut(s) | 405 |
| PagI | TCATGA | 1 cut(s) | 268 |
| PceI | AGGCCT | 1 cut(s) | 576 |
| PciSI | GCTCTTC | 1 cut(s) | 22 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 660 |
| PctI | GAATGC | 1 cut(s) | 512 |
| PdiI | GCCGGC | 1 cut(s) | 587 |
| PdmI | GAANNNNTTC | 1 cut(s) | 484 |
| PfeI | GAWTC | 1 cut(s) | 480 |
| PflFI | GACNNNGTC | 1 cut(s) | 352 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 608 |
| PkrI | GCNGC | 9 cut(s) | 138, 141, 144, 263, 305, 315, 342, 716, 731 |
| Ple19I | CGATCG | 1 cut(s) | 76 |
| PshAI | GACNNNNGTC | 1 cut(s) | 657 |
| Psp1406I | AACGTT | 1 cut(s) | 876 |
| Psp6I | CCWGG | 7 cut(s) | 131, 154, 380, 419, 510, 571, 643 |
| PspEI | GGTNACC | 1 cut(s) | 821 |
| PspFI | CCCAGC | 2 cut(s) | 360, 601 |
| PspGI | CCWGG | 7 cut(s) | 131, 154, 380, 419, 510, 571, 643 |
| PspN4I | GGNNCC | 3 cut(s) | 439, 678, 704 |
| PspPI | GGNCC | 3 cut(s) | 384, 438, 702 |
| PstI | CTGCAG | 1 cut(s) | 727 |
| PstNI | CAGNNNCTG | 1 cut(s) | 142 |
| PsuI | RGATCY | 3 cut(s) | 128, 158, 191 |
| PsyI | GACNNNGTC | 1 cut(s) | 352 |
| PvuI | CGATCG | 1 cut(s) | 76 |
| PvuII | CAGCTG | 1 cut(s) | 142 |
| RsaI | GTAC | 5 cut(s) | 174, 371, 394, 406, 541 |
| RsaNI | GTAC | 5 cut(s) | 173, 370, 393, 405, 540 |
| SalI | GTCGAC | 1 cut(s) | 355 |
| SapI | GCTCTTC | 1 cut(s) | 22 |
| SaqAI | TTAA | 3 cut(s) | 82, 288, 814 |
| SatI | GCNGC | 9 cut(s) | 137, 140, 143, 262, 304, 314, 341, 715, 730 |
| Sau3AI | GATC | 8 cut(s) | 73, 128, 158, 191, 530, 640, 718, 788 |
| Sau96I | GGNCC | 3 cut(s) | 384, 438, 702 |
| SbfI | CCTGCAGG | 1 cut(s) | 727 |
| SdaI | CCTGCAGG | 1 cut(s) | 727 |
| SduI | GDGCHC | 1 cut(s) | 92 |
| SfaNI | GCATC | 3 cut(s) | 367, 581, 607 |
| SfcI | CTRYAG | 1 cut(s) | 723 |
| SgrAI | CRCCGGYG | 1 cut(s) | 585 |
| SinI | GGWCC | 1 cut(s) | 438 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 727 |
| Sse9I | AATT | 1 cut(s) | 285 |
| SseBI | AGGCCT | 1 cut(s) | 576 |
| SspMI | CTAG | 1 cut(s) | 544 |
| StuI | AGGCCT | 1 cut(s) | 576 |
| StyI | CCWWGG | 1 cut(s) | 543 |
| TaiI | ACGT | 4 cut(s) | 278, 353, 669, 879 |
| TaqI | TCGA | 2 cut(s) | 356, 504 |
| TaqII | GACCGA | 1 cut(s) | 801 |
| TasI | AATT | 1 cut(s) | 285 |
| TauI | GCSGC | 5 cut(s) | 264, 306, 316, 717, 732 |
| TfiI | GAWTC | 1 cut(s) | 480 |
| Tru1I | TTAA | 3 cut(s) | 82, 288, 814 |
| Tru9I | TTAA | 3 cut(s) | 82, 288, 814 |
| TscAI | CASTG | 1 cut(s) | 190 |
| TseFI | GTSAC | 2 cut(s) | 164, 821 |
| TseI | GCWGC | 4 cut(s) | 136, 139, 142, 340 |
| Tsp45I | GTSAC | 2 cut(s) | 164, 821 |
| TspDTI | ATGAA | 1 cut(s) | 186 |
| TspGWI | ACGGA | 1 cut(s) | 51 |
| TspRI | CASTG | 1 cut(s) | 190 |
| Tth111I | GACNNNGTC | 1 cut(s) | 352 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 608 |
| VpaK11BI | GGWCC | 1 cut(s) | 438 |
| XceI | RCATGY | 1 cut(s) | 405 |
| XmaJI | CCTAGG | 1 cut(s) | 543 |
| XmiI | GTMKAC | 1 cut(s) | 356 |
| XmnI | GAANNNNTTC | 1 cut(s) | 484 |
| XspI | CTAG | 1 cut(s) | 544 |
| ZraI | GACGTC | 1 cut(s) | 667 |
| Zsp2I | ATGCAT | 1 cut(s) | 403 |