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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 463 |
| Acc36I | ACCTGC | 1 cut(s) | 463 |
| AccB1I | GGYRCC | 1 cut(s) | 379 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 2 cut(s) | 74, 90 |
| AclWI | GGATC | 4 cut(s) | 168, 201, 527, 637 |
| AcoI | YGGCCR | 2 cut(s) | 108, 306 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AcyI | GRCGYC | 1 cut(s) | 668 |
| AfaI | GTAC | 3 cut(s) | 176, 396, 408 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 552, 610 |
| AjnI | CCWGG | 7 cut(s) | 133, 156, 382, 421, 512, 545, 700 |
| AleI | CACNNNNGTG | 1 cut(s) | 377 |
| AluBI | AGCT | 1 cut(s) | 607 |
| AluI | AGCT | 1 cut(s) | 607 |
| Alw26I | GTCTC | 1 cut(s) | 647 |
| AlwI | GGATC | 4 cut(s) | 168, 201, 527, 637 |
| AoxI | GGCC | 8 cut(s) | 108, 154, 240, 306, 386, 554, 576, 703 |
| ApeKI | GCWGC | 5 cut(s) | 138, 141, 263, 342, 716 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 486 |
| AspLEI | GCGC | 6 cut(s) | 92, 231, 268, 368, 552, 721 |
| AspS9I | GGNCC | 4 cut(s) | 386, 440, 522, 704 |
| AsuC2I | CCSGG | 4 cut(s) | 117, 320, 647, 665 |
| AsuHPI | GGTGA | 3 cut(s) | 134, 160, 542 |
| AvaII | GGWCC | 2 cut(s) | 440, 522 |
| BanI | GGYRCC | 1 cut(s) | 379 |
| BbvI | GCAGC | 5 cut(s) | 150, 153, 250, 354, 703 |
| BccI | CCATC | 3 cut(s) | 5, 59, 253 |
| BciT130I | CCWGG | 7 cut(s) | 135, 158, 384, 423, 514, 547, 702 |
| BcnI | CCSGG | 4 cut(s) | 117, 320, 647, 665 |
| BcoDI | GTCTC | 1 cut(s) | 647 |
| BfuAI | ACCTGC | 1 cut(s) | 463 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 717 |
| BlsI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 307, 344, 718 |
| Bme18I | GGWCC | 2 cut(s) | 440, 522 |
| BmgT120I | GGNCC | 4 cut(s) | 386, 440, 522, 704 |
| BmiI | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| BmsI | GCATC | 2 cut(s) | 583, 609 |
| BoxI | GACNNNNGTC | 1 cut(s) | 659 |
| BpmI | CTGGAG | 2 cut(s) | 650, 679 |
| BpuMI | CCSGG | 4 cut(s) | 117, 320, 647, 665 |
| BsaHI | GRCGYC | 1 cut(s) | 668 |
| BsaJI | CCNNGG | 6 cut(s) | 115, 383, 422, 513, 525, 545 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 623, 653 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 552, 610 |
| Bse118I | RCCGGY | 2 cut(s) | 106, 388 |
| Bse1I | ACTGG | 2 cut(s) | 192, 633 |
| BseBI | CCWGG | 7 cut(s) | 135, 158, 384, 423, 514, 547, 702 |
| BseDI | CCNNGG | 6 cut(s) | 115, 383, 422, 513, 525, 545 |
| BseGI | GGATG | 2 cut(s) | 51, 624 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 552, 610 |
| BseMII | CTCAG | 1 cut(s) | 575 |
| BseNI | ACTGG | 2 cut(s) | 192, 633 |
| BseXI | GCAGC | 5 cut(s) | 150, 153, 250, 354, 703 |
| BseYI | CCCAGC | 2 cut(s) | 362, 603 |
| Bsh1236I | CGCG | 2 cut(s) | 74, 90 |
| Bsh1285I | CGRYCG | 1 cut(s) | 393 |
| BshFI | GGCC | 8 cut(s) | 110, 156, 242, 308, 388, 556, 578, 705 |
| BshNI | GGYRCC | 1 cut(s) | 379 |
| BsiEI | CGRYCG | 1 cut(s) | 393 |
| BsiSI | CCGG | 6 cut(s) | 107, 117, 320, 389, 646, 665 |
| BslFI | GGGAC | 2 cut(s) | 453, 647 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 552, 610 |
| BsmAI | GTCTC | 1 cut(s) | 647 |
| BsmBI | CGTCTC | 1 cut(s) | 647 |
| BsmFI | GGGAC | 2 cut(s) | 453, 647 |
| BsmI | GAATGC | 1 cut(s) | 514 |
| BsnI | GGCC | 8 cut(s) | 110, 156, 242, 308, 388, 556, 578, 705 |
| Bsp143I | GATC | 4 cut(s) | 160, 193, 532, 642 |
| BspANI | GGCC | 8 cut(s) | 110, 156, 242, 308, 388, 556, 578, 705 |
| BspCNI | CTCAG | 1 cut(s) | 574 |
| BspFNI | CGCG | 2 cut(s) | 74, 90 |
| BspLI | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| BspMI | ACCTGC | 1 cut(s) | 463 |
| BspPI | GGATC | 4 cut(s) | 168, 201, 527, 637 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 379 |
| BsrFI | RCCGGY | 2 cut(s) | 106, 388 |
| BsrI | ACTGG | 2 cut(s) | 192, 633 |
| BssAI | RCCGGY | 2 cut(s) | 106, 388 |
| BssECI | CCNNGG | 6 cut(s) | 115, 383, 422, 513, 525, 545 |
| BssMI | GATC | 4 cut(s) | 160, 193, 532, 642 |
| BssNI | GRCGYC | 1 cut(s) | 668 |
| Bst2UI | CCWGG | 7 cut(s) | 135, 158, 384, 423, 514, 547, 702 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstACI | GRCGYC | 1 cut(s) | 668 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 332, 370, 554, 729 |
| BstDEI | CTNAG | 1 cut(s) | 561 |
| BstF5I | GGATG | 2 cut(s) | 51, 624 |
| BstFNI | CGCG | 2 cut(s) | 74, 90 |
| BstHHI | GCGC | 6 cut(s) | 92, 231, 268, 368, 552, 721 |
| BstKTI | GATC | 4 cut(s) | 163, 196, 535, 645 |
| BstMAI | GTCTC | 1 cut(s) | 647 |
| BstMBI | GATC | 4 cut(s) | 160, 193, 532, 642 |
| BstMCI | CGRYCG | 1 cut(s) | 393 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 87, 144, 153, 272, 409, 562, 584, 593 |
| BstNI | CCWGG | 7 cut(s) | 135, 158, 384, 423, 514, 547, 702 |
| BstNSI | RCATGY | 1 cut(s) | 407 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 659 |
| BstUI | CGCG | 2 cut(s) | 74, 90 |
| BstV1I | GCAGC | 5 cut(s) | 150, 153, 250, 354, 703 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 8 cut(s) | 110, 156, 242, 308, 388, 556, 578, 705 |
| BtsCI | GGATG | 2 cut(s) | 51, 624 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| BveI | ACCTGC | 1 cut(s) | 463 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 332, 370, 554, 729 |
| CfoI | GCGC | 6 cut(s) | 92, 231, 268, 368, 552, 721 |
| Cfr10I | RCCGGY | 2 cut(s) | 106, 388 |
| Cfr13I | GGNCC | 4 cut(s) | 386, 440, 522, 704 |
| CseI | GACGC | 1 cut(s) | 657 |
| Csp6I | GTAC | 3 cut(s) | 175, 395, 407 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 3 cut(s) | 172, 271, 404 |
| CviQI | GTAC | 3 cut(s) | 175, 395, 407 |
| DdeI | CTNAG | 1 cut(s) | 561 |
| DpnI | GATC | 4 cut(s) | 162, 195, 534, 644 |
| DpnII | GATC | 4 cut(s) | 160, 193, 532, 642 |
| EaeI | YGGCCR | 2 cut(s) | 108, 306 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 5 cut(s) | 206, 239, 494, 525, 574 |
| Eco47I | GGWCC | 2 cut(s) | 440, 522 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoRII | CCWGG | 7 cut(s) | 133, 156, 382, 421, 512, 545, 700 |
| EcoT22I | ATGCAT | 1 cut(s) | 405 |
| Esp3I | CGTCTC | 1 cut(s) | 647 |
| FaeI | CATG | 3 cut(s) | 175, 274, 407 |
| FaiI | YATR | 8 cut(s) | 173, 201, 272, 405, 452, 542, 745, 747 |
| FaqI | GGGAC | 2 cut(s) | 453, 647 |
| FatI | CATG | 3 cut(s) | 171, 270, 403 |
| FauI | CCCGC | 3 cut(s) | 8, 79, 690 |
| FauNDI | CATATG | 1 cut(s) | 745 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 717 |
| FokI | GGATG | 2 cut(s) | 38, 631 |
| Fsp4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 717 |
| GlaI | GCGC | 6 cut(s) | 91, 230, 267, 367, 551, 720 |
| GluI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 717 |
| GsaI | CCCAGC | 2 cut(s) | 366, 607 |
| GsuI | CTGGAG | 2 cut(s) | 650, 679 |
| HaeIII | GGCC | 8 cut(s) | 110, 156, 242, 308, 388, 556, 578, 705 |
| HapII | CCGG | 6 cut(s) | 107, 117, 320, 389, 646, 665 |
| HgaI | GACGC | 1 cut(s) | 657 |
| HhaI | GCGC | 6 cut(s) | 92, 231, 268, 368, 552, 721 |
| Hin1I | GRCGYC | 1 cut(s) | 668 |
| Hin1II | CATG | 3 cut(s) | 175, 274, 407 |
| Hin6I | GCGC | 6 cut(s) | 90, 229, 266, 366, 550, 719 |
| HinP1I | GCGC | 6 cut(s) | 90, 229, 266, 366, 550, 719 |
| HincII | GTYRAC | 3 cut(s) | 85, 359, 672 |
| HindII | GTYRAC | 3 cut(s) | 85, 359, 672 |
| HinfI | GANTC | 2 cut(s) | 482, 581 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 6 cut(s) | 107, 117, 320, 389, 646, 665 |
| HphI | GGTGA | 3 cut(s) | 134, 160, 542 |
| Hpy166II | GTNNAC | 3 cut(s) | 85, 359, 672 |
| Hpy188I | TCNGA | 3 cut(s) | 67, 678, 737 |
| Hpy188III | TCNNGA | 3 cut(s) | 100, 432, 696 |
| Hpy8I | GTNNAC | 3 cut(s) | 85, 359, 672 |
| Hpy99I | CGWCG | 1 cut(s) | 593 |
| HpyCH4IV | ACGT | 3 cut(s) | 217, 277, 352 |
| HpyCH4V | TGCA | 5 cut(s) | 403, 596, 622, 727, 749 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 87, 144, 153, 272, 409, 562, 584, 593 |
| HpyF3I | CTNAG | 1 cut(s) | 561 |
| HpySE526I | ACGT | 3 cut(s) | 217, 277, 352 |
| Hsp92I | GRCGYC | 1 cut(s) | 668 |
| Hsp92II | CATG | 3 cut(s) | 175, 274, 407 |
| HspAI | GCGC | 6 cut(s) | 90, 229, 266, 366, 550, 719 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 4 cut(s) | 160, 193, 532, 642 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 2 cut(s) | 226, 631 |
| Lsp1109I | GCAGC | 5 cut(s) | 150, 153, 250, 354, 703 |
| LweI | GCATC | 2 cut(s) | 583, 609 |
| MaeII | ACGT | 3 cut(s) | 217, 277, 352 |
| MaeIII | GTNAC | 2 cut(s) | 166, 336 |
| MalI | GATC | 4 cut(s) | 162, 195, 534, 644 |
| MboI | GATC | 4 cut(s) | 160, 193, 532, 642 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MnlI | CCTC | 8 cut(s) | 73, 118, 214, 277, 465, 520, 630, 703 |
| Mph1103I | ATGCAT | 1 cut(s) | 405 |
| MroXI | GAANNNNTTC | 1 cut(s) | 486 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 377 |
| MspA1I | CMGCKG | 2 cut(s) | 412, 716 |
| MspI | CCGG | 6 cut(s) | 107, 117, 320, 389, 646, 665 |
| Mva1269I | GAATGC | 1 cut(s) | 514 |
| MvaI | CCWGG | 7 cut(s) | 135, 158, 384, 423, 514, 547, 702 |
| MvnI | CGCG | 2 cut(s) | 74, 90 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 87, 144, 153, 272, 409, 562, 584, 593 |
| NciI | CCSGG | 4 cut(s) | 117, 320, 647, 665 |
| NdeI | CATATG | 1 cut(s) | 745 |
| NdeII | GATC | 4 cut(s) | 160, 193, 532, 642 |
| NlaIII | CATG | 3 cut(s) | 175, 274, 407 |
| NlaIV | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| NmuCI | GTSAC | 2 cut(s) | 166, 336 |
| NsiI | ATGCAT | 1 cut(s) | 405 |
| NspI | RCATGY | 1 cut(s) | 407 |
| OliI | CACNNNNGTG | 1 cut(s) | 377 |
| PaqCI | CACCTGC | 1 cut(s) | 463 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PctI | GAATGC | 1 cut(s) | 514 |
| PdmI | GAANNNNTTC | 1 cut(s) | 486 |
| PfeI | GAWTC | 2 cut(s) | 482, 581 |
| PflFI | GACNNNGTC | 1 cut(s) | 354 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 307, 344, 718 |
| PshAI | GACNNNNGTC | 1 cut(s) | 659 |
| Psp6I | CCWGG | 7 cut(s) | 133, 156, 382, 421, 512, 545, 700 |
| PspFI | CCCAGC | 2 cut(s) | 362, 603 |
| PspGI | CCWGG | 7 cut(s) | 133, 156, 382, 421, 512, 545, 700 |
| PspN4I | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| PspPI | GGNCC | 4 cut(s) | 386, 440, 522, 704 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PsyI | GACNNNGTC | 1 cut(s) | 354 |
| RsaI | GTAC | 3 cut(s) | 176, 396, 408 |
| RsaNI | GTAC | 3 cut(s) | 175, 395, 407 |
| RseI | CAYNNNNRTG | 1 cut(s) | 377 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 343, 717 |
| Sau3AI | GATC | 4 cut(s) | 160, 193, 532, 642 |
| Sau96I | GGNCC | 4 cut(s) | 386, 440, 522, 704 |
| SfaNI | GCATC | 2 cut(s) | 583, 609 |
| SinI | GGWCC | 2 cut(s) | 440, 522 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 377 |
| TaiI | ACGT | 3 cut(s) | 220, 280, 355 |
| TaqI | TCGA | 1 cut(s) | 358 |
| TaqII | GACCGA | 2 cut(s) | 539, 754 |
| TauI | GCSGC | 2 cut(s) | 147, 308 |
| TfiI | GAWTC | 2 cut(s) | 482, 581 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 2 cut(s) | 166, 336 |
| TseI | GCWGC | 5 cut(s) | 138, 141, 263, 342, 716 |
| Tsp45I | GTSAC | 2 cut(s) | 166, 336 |
| TspRI | CASTG | 1 cut(s) | 192 |
| Tth111I | GACNNNGTC | 1 cut(s) | 354 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 2 cut(s) | 440, 522 |
| XceI | RCATGY | 1 cut(s) | 407 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XmnI | GAANNNNTTC | 1 cut(s) | 486 |
| Zsp2I | ATGCAT | 1 cut(s) | 405 |