Belongs to the RNase T2 family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 238 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 785 |
| Acc36I | ACCTGC | 2 cut(s) | 238, 385 |
| Acc65I | GGTACC | 1 cut(s) | 397 |
| AccB1I | GGYRCC | 2 cut(s) | 397, 650 |
| AccBSI | CCGCTC | 3 cut(s) | 495, 607, 1281 |
| AccII | CGCG | 2 cut(s) | 210, 948 |
| AclWI | GGATC | 2 cut(s) | 706, 719 |
| AcoI | YGGCCR | 2 cut(s) | 126, 252 |
| AcsI | RAATTY | 1 cut(s) | 1088 |
| AcyI | GRCGYC | 2 cut(s) | 651, 837 |
| AfaI | GTAC | 1 cut(s) | 399 |
| AfeI | AGCGCT | 1 cut(s) | 969 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 139, 247, 359, 601, 643, 1081 |
| AflII | CTTAAG | 1 cut(s) | 281 |
| AflIII | ACRYGT | 1 cut(s) | 529 |
| AgeI | ACCGGT | 1 cut(s) | 359 |
| AgsI | TTSAA | 2 cut(s) | 805, 982 |
| AjnI | CCWGG | 4 cut(s) | 147, 691, 707, 907 |
| AluBI | AGCT | 6 cut(s) | 92, 172, 280, 418, 977, 1043 |
| AluI | AGCT | 6 cut(s) | 92, 172, 280, 418, 977, 1043 |
| Alw21I | GWGCWC | 1 cut(s) | 112 |
| Alw26I | GTCTC | 1 cut(s) | 576 |
| Alw44I | GTGCAC | 1 cut(s) | 108 |
| AlwI | GGATC | 2 cut(s) | 706, 719 |
| Aor51HI | AGCGCT | 1 cut(s) | 969 |
| AoxI | GGCC | 5 cut(s) | 126, 231, 237, 252, 1001 |
| ApaLI | GTGCAC | 1 cut(s) | 108 |
| ApeKI | GCWGC | 3 cut(s) | 1040, 1195, 1275 |
| ApoI | RAATTY | 1 cut(s) | 1088 |
| AseI | ATTAAT | 1 cut(s) | 368 |
| AsiGI | ACCGGT | 1 cut(s) | 359 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 549 |
| Asp718I | GGTACC | 1 cut(s) | 397 |
| AspLEI | GCGC | 6 cut(s) | 222, 292, 653, 661, 736, 970 |
| AsuC2I | CCSGG | 2 cut(s) | 375, 638 |
| AsuHPI | GGTGA | 3 cut(s) | 55, 805, 1007 |
| AsuNHI | GCTAGC | 1 cut(s) | 1175 |
| AvaII | GGWCC | 8 cut(s) | 151, 184, 377, 431, 572, 695, 912, 1255 |
| BaeGI | GKGCMC | 1 cut(s) | 112 |
| BalI | TGGCCA | 1 cut(s) | 254 |
| BamHI | GGATCC | 1 cut(s) | 711 |
| BanI | GGYRCC | 2 cut(s) | 397, 650 |
| BanII | GRGCYC | 1 cut(s) | 147 |
| Bbv12I | GWGCWC | 1 cut(s) | 112 |
| BbvCI | CCTCAGC | 2 cut(s) | 27, 642 |
| BbvI | GCAGC | 3 cut(s) | 1052, 1207, 1287 |
| BccI | CCATC | 4 cut(s) | 66, 572, 731, 1170 |
| BciT130I | CCWGG | 4 cut(s) | 149, 693, 709, 909 |
| BclI | TGATCA | 1 cut(s) | 75 |
| BcnI | CCSGG | 2 cut(s) | 375, 638 |
| BcoDI | GTCTC | 1 cut(s) | 576 |
| BfaI | CTAG | 6 cut(s) | 179, 510, 620, 632, 971, 1176 |
| BfmI | CTRYAG | 1 cut(s) | 10 |
| BfoI | RGCGCY | 4 cut(s) | 293, 654, 662, 971 |
| BfrI | CTTAAG | 1 cut(s) | 281 |
| BfuAI | ACCTGC | 2 cut(s) | 238, 385 |
| BisI | GCNGC | 9 cut(s) | 159, 493, 769, 946, 1041, 1196, 1247, 1276, 1279 |
| BlsI | GCNGC | 9 cut(s) | 160, 494, 770, 947, 1042, 1197, 1248, 1277, 1280 |
| Bme1390I | CCNGG | 6 cut(s) | 149, 375, 638, 693, 709, 909 |
| Bme18I | GGWCC | 8 cut(s) | 151, 184, 377, 431, 572, 695, 912, 1255 |
| BmiI | GGNNCC | 9 cut(s) | 144, 399, 432, 573, 574, 652, 713, 913, 1002 |
| BmrFI | CCNGG | 6 cut(s) | 149, 375, 638, 693, 709, 909 |
| BmsI | GCATC | 2 cut(s) | 381, 600 |
| BmtI | GCTAGC | 1 cut(s) | 1179 |
| BpmI | CTGGAG | 3 cut(s) | 314, 623, 891 |
| Bpu10I | CCTNAGC | 2 cut(s) | 27, 642 |
| BpuEI | CTTGAG | 1 cut(s) | 980 |
| BpuMI | CCSGG | 2 cut(s) | 375, 638 |
| BsaHI | GRCGYC | 2 cut(s) | 651, 837 |
| BsaJI | CCNNGG | 5 cut(s) | 147, 234, 516, 908, 1288 |
| BsaWI | WCCGGW | 2 cut(s) | 359, 412 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 458, 488, 575, 605 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 139, 247, 359, 601, 643, 1081 |
| Bse118I | RCCGGY | 2 cut(s) | 124, 359 |
| Bse1I | ACTGG | 2 cut(s) | 552, 606 |
| Bse3DI | GCAATG | 1 cut(s) | 888 |
| BseBI | CCWGG | 4 cut(s) | 149, 693, 709, 909 |
| BseDI | CCNNGG | 5 cut(s) | 147, 234, 516, 908, 1288 |
| BseGI | GGATG | 4 cut(s) | 615, 723, 1030, 1071 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 139, 247, 359, 601, 643, 1081 |
| BseMI | GCAATG | 1 cut(s) | 888 |
| BseMII | CTCAG | 2 cut(s) | 18, 633 |
| BseNI | ACTGG | 2 cut(s) | 552, 606 |
| BseRI | GAGGAG | 1 cut(s) | 983 |
| BseSI | GKGCMC | 1 cut(s) | 112 |
| BseXI | GCAGC | 3 cut(s) | 1052, 1207, 1287 |
| BseYI | CCCAGC | 4 cut(s) | 134, 310, 655, 730 |
| BsgI | GTGCAG | 1 cut(s) | 1214 |
| Bsh1236I | CGCG | 2 cut(s) | 210, 948 |
| BshFI | GGCC | 5 cut(s) | 128, 233, 239, 254, 1003 |
| BshNI | GGYRCC | 2 cut(s) | 397, 650 |
| BshTI | ACCGGT | 1 cut(s) | 359 |
| BsiHKAI | GWGCWC | 1 cut(s) | 112 |
| BsiSI | CCGG | 5 cut(s) | 125, 360, 375, 413, 637 |
| BslFI | GGGAC | 4 cut(s) | 444, 518, 585, 1002 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 139, 247, 359, 601, 643, 1081 |
| BsmAI | GTCTC | 1 cut(s) | 576 |
| BsmFI | GGGAC | 4 cut(s) | 444, 518, 585, 1002 |
| BsnI | GGCC | 5 cut(s) | 128, 233, 239, 254, 1003 |
| Bsp1286I | GDGCHC | 2 cut(s) | 112, 147 |
| Bsp143I | GATC | 3 cut(s) | 75, 711, 949 |
| Bsp19I | CCATGG | 1 cut(s) | 1288 |
| BspANI | GGCC | 5 cut(s) | 128, 233, 239, 254, 1003 |
| BspCNI | CTCAG | 2 cut(s) | 19, 634 |
| BspFNI | CGCG | 2 cut(s) | 210, 948 |
| BspHI | TCATGA | 1 cut(s) | 1056 |
| BspLI | GGNNCC | 9 cut(s) | 144, 399, 432, 573, 574, 652, 713, 913, 1002 |
| BspMI | ACCTGC | 2 cut(s) | 238, 385 |
| BspOI | GCTAGC | 1 cut(s) | 1179 |
| BspPI | GGATC | 2 cut(s) | 706, 719 |
| BspT107I | GGYRCC | 2 cut(s) | 397, 650 |
| BspTI | CTTAAG | 1 cut(s) | 281 |
| BsrBI | CCGCTC | 3 cut(s) | 495, 607, 1281 |
| BsrDI | GCAATG | 1 cut(s) | 888 |
| BsrFI | RCCGGY | 2 cut(s) | 124, 359 |
| BsrI | ACTGG | 2 cut(s) | 552, 606 |
| BssAI | RCCGGY | 2 cut(s) | 124, 359 |
| BssECI | CCNNGG | 5 cut(s) | 147, 234, 516, 908, 1288 |
| BssMI | GATC | 3 cut(s) | 75, 711, 949 |
| BssNI | GRCGYC | 2 cut(s) | 651, 837 |
| BssT1I | CCWWGG | 2 cut(s) | 516, 1288 |
| Bst2UI | CCWGG | 4 cut(s) | 149, 693, 709, 909 |
| Bst4CI | ACNGT | 3 cut(s) | 11, 107, 1301 |
| Bst6I | CTCTTC | 1 cut(s) | 1205 |
| BstACI | GRCGYC | 2 cut(s) | 651, 837 |
| BstAFI | CTTAAG | 1 cut(s) | 281 |
| BstC8I | GCNNGC | 9 cut(s) | 122, 126, 174, 323, 649, 720, 1159, 1177, 1244 |
| BstDEI | CTNAG | 2 cut(s) | 27, 642 |
| BstDSI | CCRYGG | 1 cut(s) | 1288 |
| BstF5I | GGATG | 4 cut(s) | 615, 723, 1030, 1071 |
| BstFNI | CGCG | 2 cut(s) | 210, 948 |
| BstH2I | RGCGCY | 4 cut(s) | 293, 654, 662, 971 |
| BstHHI | GCGC | 6 cut(s) | 222, 292, 653, 661, 736, 970 |
| BstKTI | GATC | 3 cut(s) | 78, 714, 952 |
| BstMAI | GTCTC | 1 cut(s) | 576 |
| BstMBI | GATC | 3 cut(s) | 75, 711, 949 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 296, 311, 342, 400, 974 |
| BstNI | CCWGG | 4 cut(s) | 149, 693, 709, 909 |
| BstNSI | RCATGY | 3 cut(s) | 124, 533, 722 |
| BstSCI | CCNGG | 6 cut(s) | 147, 373, 636, 691, 707, 907 |
| BstSFI | CTRYAG | 1 cut(s) | 10 |
| BstSLI | GKGCMC | 1 cut(s) | 112 |
| BstUI | CGCG | 2 cut(s) | 210, 948 |
| BstV1I | GCAGC | 3 cut(s) | 1052, 1207, 1287 |
| BstX2I | RGATCY | 1 cut(s) | 711 |
| BstYI | RGATCY | 1 cut(s) | 711 |
| BsuRI | GGCC | 5 cut(s) | 128, 233, 239, 254, 1003 |
| BtgI | CCRYGG | 1 cut(s) | 1288 |
| BtsCI | GGATG | 4 cut(s) | 615, 723, 1030, 1071 |
| BveI | ACCTGC | 2 cut(s) | 238, 385 |
| Cac8I | GCNNGC | 9 cut(s) | 122, 126, 174, 323, 649, 720, 1159, 1177, 1244 |
| CciI | TCATGA | 1 cut(s) | 1056 |
| CfoI | GCGC | 6 cut(s) | 222, 292, 653, 661, 736, 970 |
| Cfr10I | RCCGGY | 2 cut(s) | 124, 359 |
| CseI | GACGC | 3 cut(s) | 351, 672, 845 |
| Csp6I | GTAC | 1 cut(s) | 398 |
| CspAI | ACCGGT | 1 cut(s) | 359 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 343, 378 |
| CviQI | GTAC | 1 cut(s) | 398 |
| DdeI | CTNAG | 2 cut(s) | 27, 642 |
| DinI | GGCGCC | 1 cut(s) | 652 |
| DpnI | GATC | 3 cut(s) | 77, 713, 951 |
| DpnII | GATC | 3 cut(s) | 75, 711, 949 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 785 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 785 |
| EaeI | YGGCCR | 2 cut(s) | 126, 252 |
| Eam1104I | CTCTTC | 1 cut(s) | 1205 |
| EarI | CTCTTC | 1 cut(s) | 1205 |
| EciI | GGCGGA | 4 cut(s) | 37, 485, 716, 1144 |
| Eco130I | CCWWGG | 2 cut(s) | 516, 1288 |
| Eco24I | GRGCYC | 1 cut(s) | 147 |
| Eco47I | GGWCC | 8 cut(s) | 151, 184, 377, 431, 572, 695, 912, 1255 |
| Eco47III | AGCGCT | 1 cut(s) | 969 |
| EcoO109I | RGGNCCY | 3 cut(s) | 151, 572, 695 |
| EcoRI | GAATTC | 1 cut(s) | 1088 |
| EcoRII | CCWGG | 4 cut(s) | 147, 691, 707, 907 |
| EcoT14I | CCWWGG | 2 cut(s) | 516, 1288 |
| EcoT38I | GRGCYC | 1 cut(s) | 147 |
| EgeI | GGCGCC | 1 cut(s) | 652 |
| EheI | GGCGCC | 1 cut(s) | 652 |
| ErhI | CCWWGG | 2 cut(s) | 516, 1288 |
| FaqI | GGGAC | 4 cut(s) | 444, 518, 585, 1002 |
| FauI | CCCGC | 2 cut(s) | 8, 131 |
| FauNDI | CATATG | 2 cut(s) | 792, 1099 |
| FbaI | TGATCA | 1 cut(s) | 75 |
| Fnu4HI | GCNGC | 9 cut(s) | 159, 493, 769, 946, 1041, 1196, 1247, 1276, 1279 |
| FokI | GGATG | 4 cut(s) | 622, 710, 1017, 1058 |
| FriOI | GRGCYC | 1 cut(s) | 147 |
| Fsp4HI | GCNGC | 9 cut(s) | 159, 493, 769, 946, 1041, 1196, 1247, 1276, 1279 |
| FspBI | CTAG | 6 cut(s) | 179, 510, 620, 632, 971, 1176 |
| GlaI | GCGC | 6 cut(s) | 221, 291, 652, 660, 735, 969 |
| GluI | GCNGC | 9 cut(s) | 159, 493, 769, 946, 1041, 1196, 1247, 1276, 1279 |
| GsaI | CCCAGC | 4 cut(s) | 138, 314, 659, 734 |
| GsuI | CTGGAG | 3 cut(s) | 314, 623, 891 |
| HaeII | RGCGCY | 4 cut(s) | 293, 654, 662, 971 |
| HaeIII | GGCC | 5 cut(s) | 128, 233, 239, 254, 1003 |
| HapII | CCGG | 5 cut(s) | 125, 360, 375, 413, 637 |
| HgaI | GACGC | 3 cut(s) | 351, 672, 845 |
| HhaI | GCGC | 6 cut(s) | 222, 292, 653, 661, 736, 970 |
| Hin1I | GRCGYC | 2 cut(s) | 651, 837 |
| Hin6I | GCGC | 6 cut(s) | 220, 290, 651, 659, 734, 968 |
| HinP1I | GCGC | 6 cut(s) | 220, 290, 651, 659, 734, 968 |
| HincII | GTYRAC | 1 cut(s) | 350 |
| HindII | GTYRAC | 1 cut(s) | 350 |
| HindIII | AAGCTT | 1 cut(s) | 975 |
| HinfI | GANTC | 3 cut(s) | 473, 920, 990 |
| HpaII | CCGG | 5 cut(s) | 125, 360, 375, 413, 637 |
| HphI | GGTGA | 3 cut(s) | 55, 805, 1007 |
| Hpy166II | GTNNAC | 2 cut(s) | 110, 350 |
| Hpy188I | TCNGA | 1 cut(s) | 567 |
| Hpy188III | TCNNGA | 6 cut(s) | 267, 330, 687, 953, 1057, 1166 |
| Hpy8I | GTNNAC | 2 cut(s) | 110, 350 |
| HpyAV | CCTTC | 4 cut(s) | 280, 450, 811, 1246 |
| HpyCH4III | ACNGT | 3 cut(s) | 11, 107, 1301 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 7 cut(s) | 110, 394, 613, 718, 932, 1079, 1195 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 296, 311, 342, 400, 974 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 642 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| Hsp92I | GRCGYC | 2 cut(s) | 651, 837 |
| HspAI | GCGC | 6 cut(s) | 220, 290, 651, 659, 734, 968 |
| KasI | GGCGCC | 1 cut(s) | 650 |
| KflI | GGGWCCC | 1 cut(s) | 572 |
| KpnI | GGTACC | 1 cut(s) | 401 |
| KroI | GCCGGC | 1 cut(s) | 124 |
| KroNI | GCCGGC | 1 cut(s) | 126 |
| Ksp22I | TGATCA | 1 cut(s) | 75 |
| Kzo9I | GATC | 3 cut(s) | 75, 711, 949 |
| LmnI | GCTCC | 4 cut(s) | 142, 217, 415, 604 |
| Lsp1109I | GCAGC | 3 cut(s) | 1052, 1207, 1287 |
| LweI | GCATC | 2 cut(s) | 381, 600 |
| MaeI | CTAG | 6 cut(s) | 179, 510, 620, 632, 971, 1176 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MaeIII | GTNAC | 3 cut(s) | 474, 756, 811 |
| MalI | GATC | 3 cut(s) | 77, 713, 951 |
| MbiI | CCGCTC | 3 cut(s) | 495, 607, 1281 |
| MboI | GATC | 3 cut(s) | 75, 711, 949 |
| MboII | GAAGA | 1 cut(s) | 1222 |
| MflI | RGATCY | 1 cut(s) | 711 |
| MhlI | GDGCHC | 2 cut(s) | 112, 147 |
| MlsI | TGGCCA | 1 cut(s) | 254 |
| MluCI | AATT | 3 cut(s) | 940, 1021, 1088 |
| MluNI | TGGCCA | 1 cut(s) | 254 |
| Mly113I | GGCGCC | 1 cut(s) | 651 |
| MlyI | GAGTC | 3 cut(s) | 482, 914, 984 |
| MmeI | TCCRAC | 1 cut(s) | 438 |
| Mox20I | TGGCCA | 1 cut(s) | 254 |
| MroNI | GCCGGC | 1 cut(s) | 124 |
| MroXI | GAANNNNTTC | 1 cut(s) | 549 |
| MscI | TGGCCA | 1 cut(s) | 254 |
| MseI | TTAA | 3 cut(s) | 102, 282, 368 |
| MslI | CAYNNNNRTG | 2 cut(s) | 260, 389 |
| Msp20I | TGGCCA | 1 cut(s) | 254 |
| MspA1I | CMGCKG | 4 cut(s) | 138, 403, 556, 768 |
| MspCI | CTTAAG | 1 cut(s) | 281 |
| MspI | CCGG | 5 cut(s) | 125, 360, 375, 413, 637 |
| MspR9I | CCNGG | 6 cut(s) | 149, 375, 638, 693, 709, 909 |
| MvaI | CCWGG | 4 cut(s) | 149, 693, 709, 909 |
| MvnI | CGCG | 2 cut(s) | 210, 948 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 296, 311, 342, 400, 974 |
| NaeI | GCCGGC | 1 cut(s) | 126 |
| NarI | GGCGCC | 1 cut(s) | 651 |
| NciI | CCSGG | 2 cut(s) | 375, 638 |
| NcoI | CCATGG | 1 cut(s) | 1288 |
| NdeI | CATATG | 2 cut(s) | 792, 1099 |
| NdeII | GATC | 3 cut(s) | 75, 711, 949 |
| NgoMIV | GCCGGC | 1 cut(s) | 124 |
| NheI | GCTAGC | 1 cut(s) | 1175 |
| NlaIV | GGNNCC | 9 cut(s) | 144, 399, 432, 573, 574, 652, 713, 913, 1002 |
| NmeAIII | GCCGAG | 1 cut(s) | 259 |
| NmuCI | GTSAC | 3 cut(s) | 474, 756, 811 |
| NspI | RCATGY | 3 cut(s) | 124, 533, 722 |
| PaeI | GCATGC | 2 cut(s) | 124, 722 |
| PagI | TCATGA | 1 cut(s) | 1056 |
| PaqCI | CACCTGC | 1 cut(s) | 238 |
| PciI | ACATGT | 1 cut(s) | 529 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1044 |
| PdiI | GCCGGC | 1 cut(s) | 126 |
| PdmI | GAANNNNTTC | 1 cut(s) | 549 |
| PflFI | GACNNNGTC | 2 cut(s) | 345, 1052 |
| PfoI | TCCNGGA | 1 cut(s) | 707 |
| PinAI | ACCGGT | 1 cut(s) | 359 |
| PkrI | GCNGC | 9 cut(s) | 160, 494, 770, 947, 1042, 1197, 1248, 1277, 1280 |
| PleI | GAGTC | 3 cut(s) | 481, 914, 984 |
| PluTI | GGCGCC | 1 cut(s) | 654 |
| PpsI | GAGTC | 3 cut(s) | 481, 914, 984 |
| PpuMI | RGGWCCY | 3 cut(s) | 151, 572, 695 |
| PscI | ACATGT | 1 cut(s) | 529 |
| PshBI | ATTAAT | 1 cut(s) | 368 |
| Psp5II | RGGWCCY | 3 cut(s) | 151, 572, 695 |
| Psp6I | CCWGG | 4 cut(s) | 147, 691, 707, 907 |
| PspFI | CCCAGC | 4 cut(s) | 134, 310, 655, 730 |
| PspGI | CCWGG | 4 cut(s) | 147, 691, 707, 907 |
| PspN4I | GGNNCC | 9 cut(s) | 144, 399, 432, 573, 574, 652, 713, 913, 1002 |
| PspPPI | RGGWCCY | 3 cut(s) | 151, 572, 695 |
| PsuI | RGATCY | 1 cut(s) | 711 |
| PsyI | GACNNNGTC | 2 cut(s) | 345, 1052 |
| RsaI | GTAC | 1 cut(s) | 399 |
| RsaNI | GTAC | 1 cut(s) | 398 |
| RseI | CAYNNNNRTG | 2 cut(s) | 260, 389 |
| SaqAI | TTAA | 3 cut(s) | 102, 282, 368 |
| SatI | GCNGC | 9 cut(s) | 159, 493, 769, 946, 1041, 1196, 1247, 1276, 1279 |
| Sau3AI | GATC | 3 cut(s) | 75, 711, 949 |
| SchI | GAGTC | 3 cut(s) | 482, 914, 984 |
| ScrFI | CCNGG | 6 cut(s) | 149, 375, 638, 693, 709, 909 |
| SduI | GDGCHC | 2 cut(s) | 112, 147 |
| SfaNI | GCATC | 2 cut(s) | 381, 600 |
| SfcI | CTRYAG | 1 cut(s) | 10 |
| SfoI | GGCGCC | 1 cut(s) | 652 |
| SgrAI | CRCCGGYG | 1 cut(s) | 359 |
| SinI | GGWCC | 8 cut(s) | 151, 184, 377, 431, 572, 695, 912, 1255 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 260, 389 |
| SmlI | CTYRAG | 2 cut(s) | 281, 995 |
| SmoI | CTYRAG | 2 cut(s) | 281, 995 |
| SphI | GCATGC | 2 cut(s) | 124, 722 |
| Sse9I | AATT | 3 cut(s) | 940, 1021, 1088 |
| SspDI | GGCGCC | 1 cut(s) | 650 |
| SspI | AATATT | 1 cut(s) | 1262 |
| SspMI | CTAG | 6 cut(s) | 179, 510, 620, 632, 971, 1176 |
| StyD4I | CCNGG | 6 cut(s) | 147, 373, 636, 691, 707, 907 |
| StyI | CCWWGG | 2 cut(s) | 516, 1288 |
| TaaI | ACNGT | 3 cut(s) | 11, 107, 1301 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TaqI | TCGA | 3 cut(s) | 331, 497, 831 |
| TasI | AATT | 3 cut(s) | 940, 1021, 1088 |
| TauI | GCSGC | 6 cut(s) | 161, 495, 771, 948, 1249, 1281 |
| Tru1I | TTAA | 3 cut(s) | 102, 282, 368 |
| Tru9I | TTAA | 3 cut(s) | 102, 282, 368 |
| TseFI | GTSAC | 3 cut(s) | 474, 756, 811 |
| TseI | GCWGC | 3 cut(s) | 1040, 1195, 1275 |
| Tsp45I | GTSAC | 3 cut(s) | 474, 756, 811 |
| TspDTI | ATGAA | 1 cut(s) | 1081 |
| Tth111I | GACNNNGTC | 2 cut(s) | 345, 1052 |
| Vha464I | CTTAAG | 1 cut(s) | 281 |
| VneI | GTGCAC | 1 cut(s) | 108 |
| VpaK11BI | GGWCC | 8 cut(s) | 151, 184, 377, 431, 572, 695, 912, 1255 |
| VspI | ATTAAT | 1 cut(s) | 368 |
| XapI | RAATTY | 1 cut(s) | 1088 |
| XceI | RCATGY | 3 cut(s) | 124, 533, 722 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 454, 544 |
| XmnI | GAANNNNTTC | 1 cut(s) | 549 |
| XspI | CTAG | 6 cut(s) | 179, 510, 620, 632, 971, 1176 |