21 kDa protein-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 39 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 132 |
| AgsI | TTSAA | 1 cut(s) | 142 |
| AhlI | ACTAGT | 1 cut(s) | 70 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 72 |
| AspA2I | CCTAGG | 1 cut(s) | 8 |
| AsuHPI | GGTGA | 2 cut(s) | 85, 97 |
| AvrII | CCTAGG | 1 cut(s) | 8 |
| BciVI | GTATCC | 1 cut(s) | 15 |
| BcuI | ACTAGT | 1 cut(s) | 70 |
| BfaI | CTAG | 2 cut(s) | 9, 71 |
| BfuI | GTATCC | 1 cut(s) | 15 |
| BlnI | CCTAGG | 1 cut(s) | 8 |
| BsaJI | CCNNGG | 1 cut(s) | 8 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 132 |
| BseDI | CCNNGG | 1 cut(s) | 8 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 132 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 132 |
| BspACI | CCGC | 1 cut(s) | 39 |
| BssECI | CCNNGG | 1 cut(s) | 8 |
| BssT1I | CCWWGG | 1 cut(s) | 8 |
| Bst4CI | ACNGT | 1 cut(s) | 151 |
| BstC8I | GCNNGC | 1 cut(s) | 43 |
| BsuI | GTATCC | 1 cut(s) | 15 |
| Cac8I | GCNNGC | 1 cut(s) | 43 |
| CviAII | CATG | 1 cut(s) | 127 |
| Eco130I | CCWWGG | 1 cut(s) | 8 |
| EcoT14I | CCWWGG | 1 cut(s) | 8 |
| ErhI | CCWWGG | 1 cut(s) | 8 |
| FaeI | CATG | 1 cut(s) | 130 |
| FaiI | YATR | 3 cut(s) | 78, 128, 147 |
| FatI | CATG | 1 cut(s) | 126 |
| FspBI | CTAG | 2 cut(s) | 9, 71 |
| Hin1II | CATG | 1 cut(s) | 130 |
| HphI | GGTGA | 2 cut(s) | 85, 97 |
| Hpy166II | GTNNAC | 1 cut(s) | 105 |
| Hpy188III | TCNNGA | 1 cut(s) | 52 |
| Hpy8I | GTNNAC | 1 cut(s) | 105 |
| HpyCH4III | ACNGT | 1 cut(s) | 151 |
| HpyCH4IV | ACGT | 1 cut(s) | 90 |
| HpySE526I | ACGT | 1 cut(s) | 90 |
| Hsp92II | CATG | 1 cut(s) | 130 |
| LpnPI | CCDG | 2 cut(s) | 27, 65 |
| MaeI | CTAG | 2 cut(s) | 9, 71 |
| MaeII | ACGT | 1 cut(s) | 90 |
| MaeIII | GTNAC | 2 cut(s) | 91, 121 |
| MnlI | CCTC | 2 cut(s) | 38, 91 |
| MroXI | GAANNNNTTC | 1 cut(s) | 72 |
| MspA1I | CMGCKG | 1 cut(s) | 41 |
| NlaIII | CATG | 1 cut(s) | 130 |
| NmuCI | GTSAC | 2 cut(s) | 91, 121 |
| PdmI | GAANNNNTTC | 1 cut(s) | 72 |
| SetI | ASST | 4 cut(s) | 14, 83, 93, 137 |
| SgeI | CNNG | 7 cut(s) | 21, 54, 64, 83, 110, 122, 139 |
| SpeI | ACTAGT | 1 cut(s) | 70 |
| SsiI | CCGC | 1 cut(s) | 39 |
| SspMI | CTAG | 2 cut(s) | 9, 71 |
| StyI | CCWWGG | 1 cut(s) | 8 |
| TaaI | ACNGT | 1 cut(s) | 151 |
| TaiI | ACGT | 1 cut(s) | 93 |
| TseFI | GTSAC | 2 cut(s) | 91, 121 |
| Tsp45I | GTSAC | 2 cut(s) | 91, 121 |
| TspDTI | ATGAA | 2 cut(s) | 65, 143 |
| XmaJI | CCTAGG | 1 cut(s) | 8 |
| XmnI | GAANNNNTTC | 1 cut(s) | 72 |
| XspI | CTAG | 2 cut(s) | 9, 71 |