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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 559 |
| Acc65I | GGTACC | 2 cut(s) | 544, 571 |
| AccB1I | GGYRCC | 3 cut(s) | 530, 544, 571 |
| AccI | GTMKAC | 1 cut(s) | 523 |
| AccII | CGCG | 4 cut(s) | 113, 123, 128, 245 |
| AclWI | GGATC | 3 cut(s) | 200, 226, 366 |
| AcoI | YGGCCR | 2 cut(s) | 263, 273 |
| AcuI | CTGAAG | 2 cut(s) | 186, 429 |
| AfaI | GTAC | 6 cut(s) | 12, 24, 341, 546, 561, 573 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 38, 66, 215, 533 |
| AflIII | ACRYGT | 1 cut(s) | 440 |
| AjnI | CCWGG | 1 cut(s) | 547 |
| AluBI | AGCT | 2 cut(s) | 148, 346 |
| AluI | AGCT | 2 cut(s) | 148, 346 |
| AlwI | GGATC | 3 cut(s) | 200, 226, 366 |
| AoxI | GGCC | 5 cut(s) | 263, 273, 319, 471, 551 |
| ApeKI | GCWGC | 2 cut(s) | 303, 306 |
| Asp718I | GGTACC | 2 cut(s) | 544, 571 |
| AspLEI | GCGC | 5 cut(s) | 115, 125, 130, 247, 396 |
| AspS9I | GGNCC | 3 cut(s) | 88, 551, 605 |
| AsuC2I | CCSGG | 4 cut(s) | 262, 272, 282, 528 |
| AsuHPI | GGTGA | 1 cut(s) | 299 |
| AsuII | TTCGAA | 1 cut(s) | 186 |
| AsuNHI | GCTAGC | 1 cut(s) | 346 |
| AvaII | GGWCC | 2 cut(s) | 88, 605 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 528, 555, 561, 588 |
| BanI | GGYRCC | 3 cut(s) | 530, 544, 571 |
| BbsI | GAAGAC | 1 cut(s) | 467 |
| BbvCI | CCTCAGC | 1 cut(s) | 79 |
| BbvI | GCAGC | 2 cut(s) | 315, 318 |
| BccI | CCATC | 2 cut(s) | 129, 322 |
| BciT130I | CCWGG | 1 cut(s) | 549 |
| BcnI | CCSGG | 4 cut(s) | 262, 272, 282, 528 |
| BfaI | CTAG | 2 cut(s) | 347, 513 |
| BfuAI | ACCTGC | 1 cut(s) | 559 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 68, 136 |
| BglII | AGATCT | 1 cut(s) | 325 |
| BisI | GCNGC | 4 cut(s) | 105, 126, 304, 307 |
| BlsI | GCNGC | 4 cut(s) | 106, 127, 305, 308 |
| Bme1390I | CCNGG | 5 cut(s) | 262, 272, 282, 528, 549 |
| Bme18I | GGWCC | 2 cut(s) | 88, 605 |
| BmgT120I | GGNCC | 3 cut(s) | 88, 551, 605 |
| BmiI | GGNNCC | 4 cut(s) | 532, 546, 573, 606 |
| BmrFI | CCNGG | 5 cut(s) | 262, 272, 282, 528, 549 |
| BmrI | ACTGGG | 1 cut(s) | 167 |
| BmsI | GCATC | 1 cut(s) | 148 |
| BmtI | GCTAGC | 1 cut(s) | 350 |
| BmuI | ACTGGG | 1 cut(s) | 167 |
| BpiI | GAAGAC | 1 cut(s) | 467 |
| BpmI | CTGGAG | 2 cut(s) | 80, 488 |
| Bpu10I | CCTNAGC | 1 cut(s) | 79 |
| Bpu14I | TTCGAA | 1 cut(s) | 186 |
| BpuMI | CCSGG | 4 cut(s) | 262, 272, 282, 528 |
| BsaAI | YACGTR | 1 cut(s) | 506 |
| BsaJI | CCNNGG | 4 cut(s) | 260, 270, 280, 548 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 38, 66, 215, 533 |
| Bse118I | RCCGGY | 2 cut(s) | 533, 553 |
| Bse1I | ACTGG | 2 cut(s) | 162, 357 |
| BseBI | CCWGG | 1 cut(s) | 549 |
| BseDI | CCNNGG | 4 cut(s) | 260, 270, 280, 548 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 38, 66, 215, 533 |
| BseMII | CTCAG | 2 cut(s) | 93, 579 |
| BseNI | ACTGG | 2 cut(s) | 162, 357 |
| BseRI | GAGGAG | 2 cut(s) | 401, 404 |
| BseXI | GCAGC | 2 cut(s) | 315, 318 |
| BseYI | CCCAGC | 1 cut(s) | 484 |
| Bsh1236I | CGCG | 4 cut(s) | 113, 123, 128, 245 |
| Bsh1285I | CGRYCG | 2 cut(s) | 481, 558 |
| BshFI | GGCC | 5 cut(s) | 265, 275, 321, 473, 553 |
| BshNI | GGYRCC | 3 cut(s) | 530, 544, 571 |
| BsiEI | CGRYCG | 2 cut(s) | 481, 558 |
| BsiSI | CCGG | 6 cut(s) | 262, 272, 282, 528, 534, 554 |
| BslFI | GGGAC | 1 cut(s) | 618 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 38, 66, 215, 533 |
| BsmFI | GGGAC | 1 cut(s) | 618 |
| BsnI | GGCC | 5 cut(s) | 265, 275, 321, 473, 553 |
| Bsp119I | TTCGAA | 1 cut(s) | 186 |
| Bsp143I | GATC | 5 cut(s) | 99, 205, 218, 325, 358 |
| BspANI | GGCC | 5 cut(s) | 265, 275, 321, 473, 553 |
| BspCNI | CTCAG | 2 cut(s) | 92, 580 |
| BspFNI | CGCG | 4 cut(s) | 113, 123, 128, 245 |
| BspHI | TCATGA | 1 cut(s) | 435 |
| BspLI | GGNNCC | 4 cut(s) | 532, 546, 573, 606 |
| BspMI | ACCTGC | 1 cut(s) | 559 |
| BspOI | GCTAGC | 1 cut(s) | 350 |
| BspPI | GGATC | 3 cut(s) | 200, 226, 366 |
| BspT104I | TTCGAA | 1 cut(s) | 186 |
| BspT107I | GGYRCC | 3 cut(s) | 530, 544, 571 |
| BsrFI | RCCGGY | 2 cut(s) | 533, 553 |
| BsrI | ACTGG | 2 cut(s) | 162, 357 |
| BssAI | RCCGGY | 2 cut(s) | 533, 553 |
| BssECI | CCNNGG | 4 cut(s) | 260, 270, 280, 548 |
| BssMI | GATC | 5 cut(s) | 99, 205, 218, 325, 358 |
| Bst2UI | CCWGG | 1 cut(s) | 549 |
| Bst4CI | ACNGT | 1 cut(s) | 423 |
| BstBAI | YACGTR | 1 cut(s) | 506 |
| BstBI | TTCGAA | 1 cut(s) | 186 |
| BstC8I | GCNNGC | 2 cut(s) | 348, 497 |
| BstDEI | CTNAG | 3 cut(s) | 79, 231, 588 |
| BstFNI | CGCG | 4 cut(s) | 113, 123, 128, 245 |
| BstHHI | GCGC | 5 cut(s) | 115, 125, 130, 247, 396 |
| BstKTI | GATC | 5 cut(s) | 102, 208, 221, 328, 361 |
| BstMBI | GATC | 5 cut(s) | 99, 205, 218, 325, 358 |
| BstMCI | CGRYCG | 2 cut(s) | 481, 558 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 68, 110, 136, 145, 242 |
| BstNI | CCWGG | 1 cut(s) | 549 |
| BstNSI | RCATGY | 2 cut(s) | 51, 174 |
| BstSCI | CCNGG | 5 cut(s) | 260, 270, 280, 526, 547 |
| BstUI | CGCG | 4 cut(s) | 113, 123, 128, 245 |
| BstV1I | GCAGC | 2 cut(s) | 315, 318 |
| BstV2I | GAAGAC | 1 cut(s) | 467 |
| BstX2I | RGATCY | 2 cut(s) | 325, 358 |
| BstYI | RGATCY | 2 cut(s) | 325, 358 |
| BsuRI | GGCC | 5 cut(s) | 265, 275, 321, 473, 553 |
| BtsIMutI | CAGTG | 2 cut(s) | 350, 575 |
| BveI | ACCTGC | 1 cut(s) | 559 |
| Cac8I | GCNNGC | 2 cut(s) | 348, 497 |
| CciI | TCATGA | 1 cut(s) | 435 |
| CfoI | GCGC | 5 cut(s) | 115, 125, 130, 247, 396 |
| Cfr10I | RCCGGY | 2 cut(s) | 533, 553 |
| Cfr13I | GGNCC | 3 cut(s) | 88, 551, 605 |
| CseI | GACGC | 4 cut(s) | 72, 119, 472, 574 |
| Csp6I | GTAC | 6 cut(s) | 11, 23, 340, 545, 560, 572 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 573, 608 |
| CviAII | CATG | 3 cut(s) | 48, 171, 436 |
| CviQI | GTAC | 6 cut(s) | 11, 23, 340, 545, 560, 572 |
| DdeI | CTNAG | 3 cut(s) | 79, 231, 588 |
| DpnI | GATC | 5 cut(s) | 101, 207, 220, 327, 360 |
| DpnII | GATC | 5 cut(s) | 99, 205, 218, 325, 358 |
| EaeI | YGGCCR | 2 cut(s) | 263, 273 |
| EciI | GGCGGA | 2 cut(s) | 198, 371 |
| Eco47I | GGWCC | 2 cut(s) | 88, 605 |
| Eco57I | CTGAAG | 2 cut(s) | 186, 429 |
| EcoRII | CCWGG | 1 cut(s) | 547 |
| FaeI | CATG | 3 cut(s) | 51, 174, 439 |
| FaiI | YATR | 6 cut(s) | 49, 172, 338, 366, 437, 616 |
| FaqI | GGGAC | 1 cut(s) | 618 |
| FatI | CATG | 3 cut(s) | 47, 170, 435 |
| FauI | CCCGC | 1 cut(s) | 170 |
| FblI | GTMKAC | 1 cut(s) | 523 |
| Fnu4HI | GCNGC | 4 cut(s) | 105, 126, 304, 307 |
| Fsp4HI | GCNGC | 4 cut(s) | 105, 126, 304, 307 |
| FspBI | CTAG | 2 cut(s) | 347, 513 |
| GlaI | GCGC | 5 cut(s) | 114, 124, 129, 246, 395 |
| GluI | GCNGC | 4 cut(s) | 105, 126, 304, 307 |
| GsaI | CCCAGC | 1 cut(s) | 488 |
| GsuI | CTGGAG | 2 cut(s) | 80, 488 |
| HaeIII | GGCC | 5 cut(s) | 265, 275, 321, 473, 553 |
| HapII | CCGG | 6 cut(s) | 262, 272, 282, 528, 534, 554 |
| HgaI | GACGC | 4 cut(s) | 72, 119, 472, 574 |
| HhaI | GCGC | 5 cut(s) | 115, 125, 130, 247, 396 |
| Hin1II | CATG | 3 cut(s) | 51, 174, 439 |
| Hin6I | GCGC | 5 cut(s) | 113, 123, 128, 245, 394 |
| HinP1I | GCGC | 5 cut(s) | 113, 123, 128, 245, 394 |
| HincII | GTYRAC | 3 cut(s) | 228, 240, 524 |
| HindII | GTYRAC | 3 cut(s) | 228, 240, 524 |
| HindIII | AAGCTT | 1 cut(s) | 146 |
| HpaI | GTTAAC | 2 cut(s) | 228, 240 |
| HpaII | CCGG | 6 cut(s) | 262, 272, 282, 528, 534, 554 |
| HphI | GGTGA | 1 cut(s) | 299 |
| Hpy166II | GTNNAC | 3 cut(s) | 228, 240, 524 |
| Hpy188I | TCNGA | 3 cut(s) | 88, 144, 153 |
| Hpy188III | TCNNGA | 2 cut(s) | 97, 436 |
| Hpy8I | GTNNAC | 3 cut(s) | 228, 240, 524 |
| Hpy99I | CGWCG | 2 cut(s) | 88, 113 |
| HpyCH4III | ACNGT | 1 cut(s) | 423 |
| HpyCH4IV | ACGT | 3 cut(s) | 382, 442, 505 |
| HpyCH4V | TGCA | 1 cut(s) | 51 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 68, 110, 136, 145, 242 |
| HpyF3I | CTNAG | 3 cut(s) | 79, 231, 588 |
| HpySE526I | ACGT | 3 cut(s) | 382, 442, 505 |
| Hsp92II | CATG | 3 cut(s) | 51, 174, 439 |
| HspAI | GCGC | 5 cut(s) | 113, 123, 128, 245, 394 |
| KpnI | GGTACC | 2 cut(s) | 548, 575 |
| KspAI | GTTAAC | 2 cut(s) | 228, 240 |
| Kzo9I | GATC | 5 cut(s) | 99, 205, 218, 325, 358 |
| LmnI | GCTCC | 1 cut(s) | 391 |
| Lsp1109I | GCAGC | 2 cut(s) | 315, 318 |
| LweI | GCATC | 1 cut(s) | 148 |
| MaeI | CTAG | 2 cut(s) | 347, 513 |
| MaeII | ACGT | 3 cut(s) | 382, 442, 505 |
| MaeIII | GTNAC | 2 cut(s) | 331, 501 |
| MalI | GATC | 5 cut(s) | 101, 207, 220, 327, 360 |
| MboI | GATC | 5 cut(s) | 99, 205, 218, 325, 358 |
| MboII | GAAGA | 2 cut(s) | 353, 472 |
| MflI | RGATCY | 2 cut(s) | 325, 358 |
| MluCI | AATT | 1 cut(s) | 27 |
| MmeI | TCCRAC | 2 cut(s) | 56, 66 |
| MnlI | CCTC | 8 cut(s) | 88, 104, 126, 283, 379, 382, 442, 463 |
| MseI | TTAA | 2 cut(s) | 227, 239 |
| MspI | CCGG | 6 cut(s) | 262, 272, 282, 528, 534, 554 |
| MspR9I | CCNGG | 5 cut(s) | 262, 272, 282, 528, 549 |
| MteI | GCGCNGCGC | 1 cut(s) | 126 |
| MvaI | CCWGG | 1 cut(s) | 549 |
| MvnI | CGCG | 4 cut(s) | 113, 123, 128, 245 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 68, 110, 136, 145, 242 |
| NciI | CCSGG | 4 cut(s) | 262, 272, 282, 528 |
| NdeII | GATC | 5 cut(s) | 99, 205, 218, 325, 358 |
| NheI | GCTAGC | 1 cut(s) | 346 |
| NlaIII | CATG | 3 cut(s) | 51, 174, 439 |
| NlaIV | GGNNCC | 4 cut(s) | 532, 546, 573, 606 |
| NmuCI | GTSAC | 2 cut(s) | 331, 501 |
| NspI | RCATGY | 2 cut(s) | 51, 174 |
| NspV | TTCGAA | 1 cut(s) | 186 |
| PagI | TCATGA | 1 cut(s) | 435 |
| PkrI | GCNGC | 4 cut(s) | 106, 127, 305, 308 |
| Ppu21I | YACGTR | 1 cut(s) | 506 |
| Psp6I | CCWGG | 1 cut(s) | 547 |
| PspFI | CCCAGC | 1 cut(s) | 484 |
| PspGI | CCWGG | 1 cut(s) | 547 |
| PspN4I | GGNNCC | 4 cut(s) | 532, 546, 573, 606 |
| PspPI | GGNCC | 3 cut(s) | 88, 551, 605 |
| PsuI | RGATCY | 2 cut(s) | 325, 358 |
| RsaI | GTAC | 6 cut(s) | 12, 24, 341, 546, 561, 573 |
| RsaNI | GTAC | 6 cut(s) | 11, 23, 340, 545, 560, 572 |
| SalI | GTCGAC | 1 cut(s) | 522 |
| SaqAI | TTAA | 2 cut(s) | 227, 239 |
| SatI | GCNGC | 4 cut(s) | 105, 126, 304, 307 |
| Sau3AI | GATC | 5 cut(s) | 99, 205, 218, 325, 358 |
| Sau96I | GGNCC | 3 cut(s) | 88, 551, 605 |
| ScrFI | CCNGG | 5 cut(s) | 262, 272, 282, 528, 549 |
| SfaNI | GCATC | 1 cut(s) | 148 |
| SfuI | TTCGAA | 1 cut(s) | 186 |
| SinI | GGWCC | 2 cut(s) | 88, 605 |
| Sse9I | AATT | 1 cut(s) | 27 |
| SspMI | CTAG | 2 cut(s) | 347, 513 |
| StyD4I | CCNGG | 5 cut(s) | 260, 270, 280, 526, 547 |
| TaaI | ACNGT | 1 cut(s) | 423 |
| TaiI | ACGT | 3 cut(s) | 385, 445, 508 |
| TaqI | TCGA | 2 cut(s) | 186, 523 |
| TasI | AATT | 1 cut(s) | 27 |
| TauI | GCSGC | 2 cut(s) | 107, 128 |
| Tru1I | TTAA | 2 cut(s) | 227, 239 |
| Tru9I | TTAA | 2 cut(s) | 227, 239 |
| TscAI | CASTG | 2 cut(s) | 357, 582 |
| TseFI | GTSAC | 2 cut(s) | 331, 501 |
| TseI | GCWGC | 2 cut(s) | 303, 306 |
| Tsp45I | GTSAC | 2 cut(s) | 331, 501 |
| TspDTI | ATGAA | 1 cut(s) | 353 |
| TspRI | CASTG | 2 cut(s) | 357, 582 |
| VpaK11BI | GGWCC | 2 cut(s) | 88, 605 |
| XceI | RCATGY | 2 cut(s) | 51, 174 |
| XmiI | GTMKAC | 1 cut(s) | 523 |
| XspI | CTAG | 2 cut(s) | 347, 513 |