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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 394, 569 |
| Acc65I | GGTACC | 2 cut(s) | 379, 406 |
| AccB1I | GGYRCC | 2 cut(s) | 379, 406 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccBSI | CCGCTC | 1 cut(s) | 170 |
| AccII | CGCG | 2 cut(s) | 90, 277 |
| AclWI | GGATC | 2 cut(s) | 168, 201 |
| AcuI | CTGAAG | 3 cut(s) | 48, 264, 546 |
| AfaI | GTAC | 2 cut(s) | 381, 408 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 151, 368, 610 |
| AflIII | ACRYGT | 1 cut(s) | 556 |
| AgsI | TTSAA | 1 cut(s) | 100 |
| AjnI | CCWGG | 4 cut(s) | 156, 347, 382, 573 |
| AluBI | AGCT | 1 cut(s) | 181 |
| AluI | AGCT | 1 cut(s) | 181 |
| Alw26I | GTCTC | 1 cut(s) | 119 |
| AlwI | GGATC | 2 cut(s) | 168, 201 |
| AoxI | GGCC | 3 cut(s) | 154, 359, 386 |
| Asp718I | GGTACC | 2 cut(s) | 379, 406 |
| AspLEI | GCGC | 3 cut(s) | 92, 325, 552 |
| AspS9I | GGNCC | 6 cut(s) | 40, 154, 360, 386, 415, 440 |
| AsuC2I | CCSGG | 2 cut(s) | 117, 153 |
| AsuHPI | GGTGA | 1 cut(s) | 134 |
| AvaII | GGWCC | 3 cut(s) | 40, 415, 440 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 363, 396 |
| BanI | GGYRCC | 2 cut(s) | 379, 406 |
| BccI | CCATC | 2 cut(s) | 52, 430 |
| BciT130I | CCWGG | 4 cut(s) | 158, 349, 384, 575 |
| BcnI | CCSGG | 2 cut(s) | 117, 153 |
| BcoDI | GTCTC | 1 cut(s) | 119 |
| BfaI | CTAG | 1 cut(s) | 6 |
| BfuAI | ACCTGC | 2 cut(s) | 394, 569 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 2 cut(s) | 145, 168 |
| BlsI | GCNGC | 2 cut(s) | 146, 169 |
| Bme1390I | CCNGG | 6 cut(s) | 117, 153, 158, 349, 384, 575 |
| Bme18I | GGWCC | 3 cut(s) | 40, 415, 440 |
| BmgT120I | GGNCC | 6 cut(s) | 40, 154, 360, 386, 415, 440 |
| BmiI | GGNNCC | 6 cut(s) | 42, 381, 408, 417, 441, 583 |
| BmrFI | CCNGG | 6 cut(s) | 117, 153, 158, 349, 384, 575 |
| BmrI | ACTGGG | 1 cut(s) | 65 |
| BmsI | GCATC | 2 cut(s) | 390, 609 |
| BmuI | ACTGGG | 1 cut(s) | 65 |
| BpuMI | CCSGG | 2 cut(s) | 117, 153 |
| BsaAI | YACGTR | 1 cut(s) | 341 |
| BsaJI | CCNNGG | 3 cut(s) | 115, 383, 545 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 467, 497 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 151, 368, 610 |
| Bse118I | RCCGGY | 1 cut(s) | 587 |
| Bse1I | ACTGG | 5 cut(s) | 71, 192, 373, 389, 615 |
| BseBI | CCWGG | 4 cut(s) | 158, 349, 384, 575 |
| BseDI | CCNNGG | 3 cut(s) | 115, 383, 545 |
| BseGI | GGATG | 3 cut(s) | 44, 441, 624 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 151, 368, 610 |
| BseMII | CTCAG | 1 cut(s) | 519 |
| BseNI | ACTGG | 5 cut(s) | 71, 192, 373, 389, 615 |
| BseYI | CCCAGC | 1 cut(s) | 106 |
| Bsh1236I | CGCG | 2 cut(s) | 90, 277 |
| BshFI | GGCC | 3 cut(s) | 156, 361, 388 |
| BshNI | GGYRCC | 2 cut(s) | 379, 406 |
| BsiSI | CCGG | 3 cut(s) | 117, 152, 588 |
| BslFI | GGGAC | 1 cut(s) | 453 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 151, 368, 610 |
| BsmAI | GTCTC | 1 cut(s) | 119 |
| BsmFI | GGGAC | 1 cut(s) | 453 |
| BsnI | GGCC | 3 cut(s) | 156, 361, 388 |
| Bsp143I | GATC | 2 cut(s) | 160, 193 |
| BspANI | GGCC | 3 cut(s) | 156, 361, 388 |
| BspCNI | CTCAG | 1 cut(s) | 518 |
| BspFNI | CGCG | 2 cut(s) | 90, 277 |
| BspLI | GGNNCC | 6 cut(s) | 42, 381, 408, 417, 441, 583 |
| BspMI | ACCTGC | 2 cut(s) | 394, 569 |
| BspPI | GGATC | 2 cut(s) | 168, 201 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 2 cut(s) | 379, 406 |
| BsrBI | CCGCTC | 1 cut(s) | 170 |
| BsrFI | RCCGGY | 1 cut(s) | 587 |
| BsrI | ACTGG | 5 cut(s) | 71, 192, 373, 389, 615 |
| BssAI | RCCGGY | 1 cut(s) | 587 |
| BssECI | CCNNGG | 3 cut(s) | 115, 383, 545 |
| BssMI | GATC | 2 cut(s) | 160, 193 |
| BssT1I | CCWWGG | 1 cut(s) | 545 |
| Bst2UI | CCWGG | 4 cut(s) | 158, 349, 384, 575 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 216 |
| BstBAI | YACGTR | 1 cut(s) | 341 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 183, 589 |
| BstDEI | CTNAG | 1 cut(s) | 505 |
| BstF5I | GGATG | 3 cut(s) | 44, 441, 624 |
| BstFNI | CGCG | 2 cut(s) | 90, 277 |
| BstHHI | GCGC | 3 cut(s) | 92, 325, 552 |
| BstKTI | GATC | 2 cut(s) | 163, 196 |
| BstMAI | GTCTC | 1 cut(s) | 119 |
| BstMBI | GATC | 2 cut(s) | 160, 193 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 87, 144, 153, 274, 320, 358, 409, 584, 593 |
| BstNI | CCWGG | 4 cut(s) | 158, 349, 384, 575 |
| BstNSI | RCATGY | 1 cut(s) | 560 |
| BstSCI | CCNGG | 6 cut(s) | 115, 151, 156, 347, 382, 573 |
| BstUI | CGCG | 2 cut(s) | 90, 277 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 73 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 3 cut(s) | 156, 361, 388 |
| BtsCI | GGATG | 3 cut(s) | 44, 441, 624 |
| BtsIMutI | CAGTG | 3 cut(s) | 78, 185, 366 |
| BveI | ACCTGC | 2 cut(s) | 394, 569 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 183, 589 |
| CfoI | GCGC | 3 cut(s) | 92, 325, 552 |
| Cfr10I | RCCGGY | 1 cut(s) | 587 |
| Cfr13I | GGNCC | 6 cut(s) | 40, 154, 360, 386, 415, 440 |
| CseI | GACGC | 1 cut(s) | 307 |
| Csp6I | GTAC | 2 cut(s) | 380, 407 |
| CviAII | CATG | 1 cut(s) | 557 |
| CviQI | GTAC | 2 cut(s) | 380, 407 |
| DdeI | CTNAG | 1 cut(s) | 505 |
| DpnI | GATC | 2 cut(s) | 162, 195 |
| DpnII | GATC | 2 cut(s) | 160, 193 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 216 |
| EarI | CTCTTC | 2 cut(s) | 24, 216 |
| EciI | GGCGGA | 5 cut(s) | 37, 53, 206, 494, 574 |
| Eco130I | CCWWGG | 1 cut(s) | 545 |
| Eco47I | GGWCC | 3 cut(s) | 40, 415, 440 |
| Eco57I | CTGAAG | 3 cut(s) | 48, 264, 546 |
| EcoRII | CCWGG | 4 cut(s) | 156, 347, 382, 573 |
| EcoT14I | CCWWGG | 1 cut(s) | 545 |
| ErhI | CCWWGG | 1 cut(s) | 545 |
| FaeI | CATG | 1 cut(s) | 560 |
| FaiI | YATR | 4 cut(s) | 175, 395, 452, 558 |
| FaqI | GGGAC | 1 cut(s) | 453 |
| FatI | CATG | 1 cut(s) | 556 |
| Fnu4HI | GCNGC | 2 cut(s) | 145, 168 |
| FokI | GGATG | 3 cut(s) | 31, 448, 631 |
| Fsp4HI | GCNGC | 2 cut(s) | 145, 168 |
| FspBI | CTAG | 1 cut(s) | 6 |
| GlaI | GCGC | 3 cut(s) | 91, 324, 551 |
| GluI | GCNGC | 2 cut(s) | 145, 168 |
| GsaI | CCCAGC | 1 cut(s) | 110 |
| HaeIII | GGCC | 3 cut(s) | 156, 361, 388 |
| HapII | CCGG | 3 cut(s) | 117, 152, 588 |
| HgaI | GACGC | 1 cut(s) | 307 |
| HhaI | GCGC | 3 cut(s) | 92, 325, 552 |
| Hin1II | CATG | 1 cut(s) | 560 |
| Hin6I | GCGC | 3 cut(s) | 90, 323, 550 |
| HinP1I | GCGC | 3 cut(s) | 90, 323, 550 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HinfI | GANTC | 1 cut(s) | 482 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 3 cut(s) | 117, 152, 588 |
| HphI | GGTGA | 1 cut(s) | 134 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 297 |
| Hpy188I | TCNGA | 1 cut(s) | 508 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 297 |
| HpyAV | CCTTC | 2 cut(s) | 232, 521 |
| HpyCH4IV | ACGT | 2 cut(s) | 340, 374 |
| HpyCH4V | TGCA | 2 cut(s) | 403, 622 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 87, 144, 153, 274, 320, 358, 409, 584, 593 |
| HpyF3I | CTNAG | 1 cut(s) | 505 |
| HpySE526I | ACGT | 2 cut(s) | 340, 374 |
| Hsp92II | CATG | 1 cut(s) | 560 |
| HspAI | GCGC | 3 cut(s) | 90, 323, 550 |
| KpnI | GGTACC | 2 cut(s) | 383, 410 |
| KroI | GCCGGC | 1 cut(s) | 587 |
| KroNI | GCCGGC | 1 cut(s) | 589 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 2 cut(s) | 160, 193 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 469 |
| LweI | GCATC | 2 cut(s) | 390, 609 |
| MaeI | CTAG | 1 cut(s) | 6 |
| MaeII | ACGT | 2 cut(s) | 340, 374 |
| MaeIII | GTNAC | 1 cut(s) | 483 |
| MalI | GATC | 2 cut(s) | 162, 195 |
| MbiI | CCGCTC | 1 cut(s) | 170 |
| MboI | GATC | 2 cut(s) | 160, 193 |
| MboII | GAAGA | 3 cut(s) | 41, 188, 233 |
| MfeI | CAATTG | 1 cut(s) | 626 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MluCI | AATT | 2 cut(s) | 532, 626 |
| MlyI | GAGTC | 1 cut(s) | 491 |
| MmeI | TCCRAC | 2 cut(s) | 90, 447 |
| MnlI | CCTC | 5 cut(s) | 73, 217, 469, 501, 507 |
| MroNI | GCCGGC | 1 cut(s) | 587 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 398 |
| MspA1I | CMGCKG | 2 cut(s) | 412, 565 |
| MspI | CCGG | 3 cut(s) | 117, 152, 588 |
| MspR9I | CCNGG | 6 cut(s) | 117, 153, 158, 349, 384, 575 |
| MunI | CAATTG | 1 cut(s) | 626 |
| MvaI | CCWGG | 4 cut(s) | 158, 349, 384, 575 |
| MvnI | CGCG | 2 cut(s) | 90, 277 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 87, 144, 153, 274, 320, 358, 409, 584, 593 |
| NaeI | GCCGGC | 1 cut(s) | 589 |
| NciI | CCSGG | 2 cut(s) | 117, 153 |
| NdeII | GATC | 2 cut(s) | 160, 193 |
| NgoMIV | GCCGGC | 1 cut(s) | 587 |
| NlaIII | CATG | 1 cut(s) | 560 |
| NlaIV | GGNNCC | 6 cut(s) | 42, 381, 408, 417, 441, 583 |
| NmuCI | GTSAC | 1 cut(s) | 483 |
| NspI | RCATGY | 1 cut(s) | 560 |
| PciI | ACATGT | 1 cut(s) | 556 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PdiI | GCCGGC | 1 cut(s) | 589 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 2 cut(s) | 146, 169 |
| PleI | GAGTC | 1 cut(s) | 490 |
| PpsI | GAGTC | 1 cut(s) | 490 |
| Ppu21I | YACGTR | 1 cut(s) | 341 |
| PscI | ACATGT | 1 cut(s) | 556 |
| Psp6I | CCWGG | 4 cut(s) | 156, 347, 382, 573 |
| PspFI | CCCAGC | 1 cut(s) | 106 |
| PspGI | CCWGG | 4 cut(s) | 156, 347, 382, 573 |
| PspN4I | GGNNCC | 6 cut(s) | 42, 381, 408, 417, 441, 583 |
| PspPI | GGNCC | 6 cut(s) | 40, 154, 360, 386, 415, 440 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| RsaI | GTAC | 2 cut(s) | 381, 408 |
| RsaNI | GTAC | 2 cut(s) | 380, 407 |
| RseI | CAYNNNNRTG | 1 cut(s) | 398 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 2 cut(s) | 145, 168 |
| Sau3AI | GATC | 2 cut(s) | 160, 193 |
| Sau96I | GGNCC | 6 cut(s) | 40, 154, 360, 386, 415, 440 |
| SchI | GAGTC | 1 cut(s) | 491 |
| ScrFI | CCNGG | 6 cut(s) | 117, 153, 158, 349, 384, 575 |
| SetI | ASST | 7 cut(s) | 183, 343, 377, 408, 424, 493, 583 |
| SfaNI | GCATC | 2 cut(s) | 390, 609 |
| SinI | GGWCC | 3 cut(s) | 40, 415, 440 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 398 |
| Sse9I | AATT | 2 cut(s) | 532, 626 |
| SspMI | CTAG | 1 cut(s) | 6 |
| StyD4I | CCNGG | 6 cut(s) | 115, 151, 156, 347, 382, 573 |
| StyI | CCWWGG | 1 cut(s) | 545 |
| TaiI | ACGT | 2 cut(s) | 343, 377 |
| TasI | AATT | 2 cut(s) | 532, 626 |
| TauI | GCSGC | 2 cut(s) | 147, 170 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 3 cut(s) | 78, 192, 373 |
| TseFI | GTSAC | 1 cut(s) | 483 |
| Tsp45I | GTSAC | 1 cut(s) | 483 |
| TspDTI | ATGAA | 1 cut(s) | 300 |
| TspRI | CASTG | 3 cut(s) | 78, 192, 373 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 3 cut(s) | 40, 415, 440 |
| XceI | RCATGY | 1 cut(s) | 560 |
| XspI | CTAG | 1 cut(s) | 6 |