No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 4, 16 |
| AccB1I | GGYRCC | 2 cut(s) | 4, 16 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 151 |
| AccBSI | CCGCTC | 2 cut(s) | 71, 667 |
| AccII | CGCG | 5 cut(s) | 107, 122, 250, 330, 855 |
| AclWI | GGATC | 2 cut(s) | 441, 725 |
| AcoI | YGGCCR | 3 cut(s) | 54, 501, 892 |
| AcyI | GRCGYC | 1 cut(s) | 264 |
| AfaI | GTAC | 4 cut(s) | 6, 18, 616, 734 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 32, 151, 219, 317, 709, 714 |
| AgsI | TTSAA | 1 cut(s) | 841 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 268 |
| AjnI | CCWGG | 8 cut(s) | 56, 78, 373, 391, 708, 735, 858, 863 |
| AleI | CACNNNNGTG | 1 cut(s) | 509 |
| AluBI | AGCT | 5 cut(s) | 142, 421, 529, 590, 623 |
| AluI | AGCT | 5 cut(s) | 142, 421, 529, 590, 623 |
| Alw26I | GTCTC | 3 cut(s) | 258, 276, 359 |
| AlwI | GGATC | 2 cut(s) | 441, 725 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 151 |
| AoxI | GGCC | 8 cut(s) | 54, 117, 394, 480, 486, 501, 861, 892 |
| ApeKI | GCWGC | 2 cut(s) | 381, 575 |
| Asp718I | GGTACC | 2 cut(s) | 4, 16 |
| AspLEI | GCGC | 2 cut(s) | 109, 332 |
| AspS9I | GGNCC | 5 cut(s) | 82, 117, 487, 603, 867 |
| AsuC2I | CCSGG | 4 cut(s) | 347, 357, 560, 810 |
| AsuHPI | GGTGA | 3 cut(s) | 202, 374, 654 |
| AsuNHI | GCTAGC | 1 cut(s) | 590 |
| AvaII | GGWCC | 3 cut(s) | 82, 603, 867 |
| AxyI | CCTNAGG | 1 cut(s) | 676 |
| BalI | TGGCCA | 1 cut(s) | 503 |
| BanI | GGYRCC | 2 cut(s) | 4, 16 |
| BbsI | GAAGAC | 2 cut(s) | 200, 243 |
| BbvI | GCAGC | 2 cut(s) | 393, 562 |
| BccI | CCATC | 1 cut(s) | 123 |
| BceAI | ACGGC | 2 cut(s) | 69, 879 |
| BciT130I | CCWGG | 8 cut(s) | 58, 80, 375, 393, 710, 737, 860, 865 |
| BcnI | CCSGG | 4 cut(s) | 347, 357, 560, 810 |
| BcoDI | GTCTC | 3 cut(s) | 258, 276, 359 |
| BfaI | CTAG | 3 cut(s) | 11, 591, 741 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 62 |
| BisI | GCNGC | 4 cut(s) | 110, 120, 382, 576 |
| BlsI | GCNGC | 4 cut(s) | 111, 121, 383, 577 |
| Bme18I | GGWCC | 3 cut(s) | 82, 603, 867 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 268 |
| BmgT120I | GGNCC | 5 cut(s) | 82, 117, 487, 603, 867 |
| BmiI | GGNNCC | 4 cut(s) | 6, 18, 604, 824 |
| BmsI | GCATC | 4 cut(s) | 142, 232, 746, 772 |
| BmtI | GCTAGC | 1 cut(s) | 594 |
| BpiI | GAAGAC | 2 cut(s) | 200, 243 |
| BpmI | CTGGAG | 2 cut(s) | 128, 563 |
| BpuEI | CTTGAG | 1 cut(s) | 653 |
| BpuMI | CCSGG | 4 cut(s) | 347, 357, 560, 810 |
| BsaHI | GRCGYC | 1 cut(s) | 264 |
| BsaI | GGTCTC | 1 cut(s) | 258 |
| BsaJI | CCNNGG | 8 cut(s) | 30, 78, 113, 345, 355, 373, 483, 708 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 238, 268, 630, 660 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 32, 151, 219, 317, 709, 714 |
| Bse118I | RCCGGY | 2 cut(s) | 750, 902 |
| Bse1I | ACTGG | 1 cut(s) | 778 |
| Bse21I | CCTNAGG | 1 cut(s) | 676 |
| BseBI | CCWGG | 8 cut(s) | 58, 80, 375, 393, 710, 737, 860, 865 |
| BseDI | CCNNGG | 8 cut(s) | 30, 78, 113, 345, 355, 373, 483, 708 |
| BseGI | GGATG | 1 cut(s) | 787 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 32, 151, 219, 317, 709, 714 |
| BseMII | CTCAG | 1 cut(s) | 690 |
| BseNI | ACTGG | 1 cut(s) | 778 |
| BseXI | GCAGC | 2 cut(s) | 393, 562 |
| BseYI | CCCAGC | 3 cut(s) | 310, 619, 827 |
| BsgI | GTGCAG | 2 cut(s) | 561, 639 |
| Bsh1236I | CGCG | 5 cut(s) | 107, 122, 250, 330, 855 |
| BshFI | GGCC | 8 cut(s) | 56, 119, 396, 482, 488, 503, 863, 894 |
| BshNI | GGYRCC | 2 cut(s) | 4, 16 |
| BsiSI | CCGG | 6 cut(s) | 347, 357, 560, 751, 809, 903 |
| BslFI | GGGAC | 4 cut(s) | 157, 499, 555, 616 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 32, 151, 219, 317, 709, 714 |
| BsmAI | GTCTC | 3 cut(s) | 258, 276, 359 |
| BsmFI | GGGAC | 4 cut(s) | 157, 499, 555, 616 |
| BsnI | GGCC | 8 cut(s) | 56, 119, 396, 482, 488, 503, 863, 894 |
| Bso31I | GGTCTC | 1 cut(s) | 258 |
| Bsp143I | GATC | 4 cut(s) | 93, 280, 433, 717 |
| Bsp68I | TCGCGA | 1 cut(s) | 855 |
| BspANI | GGCC | 8 cut(s) | 56, 119, 396, 482, 488, 503, 863, 894 |
| BspCNI | CTCAG | 1 cut(s) | 689 |
| BspFNI | CGCG | 5 cut(s) | 107, 122, 250, 330, 855 |
| BspLI | GGNNCC | 4 cut(s) | 6, 18, 604, 824 |
| BspOI | GCTAGC | 1 cut(s) | 594 |
| BspPI | GGATC | 2 cut(s) | 441, 725 |
| BspT107I | GGYRCC | 2 cut(s) | 4, 16 |
| BspTNI | GGTCTC | 1 cut(s) | 258 |
| BsrBI | CCGCTC | 2 cut(s) | 71, 667 |
| BsrFI | RCCGGY | 2 cut(s) | 750, 902 |
| BsrI | ACTGG | 1 cut(s) | 778 |
| BssAI | RCCGGY | 2 cut(s) | 750, 902 |
| BssECI | CCNNGG | 8 cut(s) | 30, 78, 113, 345, 355, 373, 483, 708 |
| BssMI | GATC | 4 cut(s) | 93, 280, 433, 717 |
| BssNI | GRCGYC | 1 cut(s) | 264 |
| BssT1I | CCWWGG | 2 cut(s) | 30, 483 |
| Bst2UI | CCWGG | 8 cut(s) | 58, 80, 375, 393, 710, 737, 860, 865 |
| BstACI | GRCGYC | 1 cut(s) | 264 |
| BstC8I | GCNNGC | 5 cut(s) | 63, 423, 592, 752, 892 |
| BstDEI | CTNAG | 1 cut(s) | 676 |
| BstDSI | CCRYGG | 1 cut(s) | 113 |
| BstEII | GGTNACC | 1 cut(s) | 37 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 217, 707 |
| BstF5I | GGATG | 1 cut(s) | 787 |
| BstFNI | CGCG | 5 cut(s) | 107, 122, 250, 330, 855 |
| BstHHI | GCGC | 2 cut(s) | 109, 332 |
| BstKTI | GATC | 4 cut(s) | 96, 283, 436, 720 |
| BstMAI | GTCTC | 3 cut(s) | 258, 276, 359 |
| BstMBI | GATC | 4 cut(s) | 93, 280, 433, 717 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 62, 139, 148, 327, 336, 756 |
| BstNI | CCWGG | 8 cut(s) | 58, 80, 375, 393, 710, 737, 860, 865 |
| BstPI | GGTNACC | 1 cut(s) | 37 |
| BstUI | CGCG | 5 cut(s) | 107, 122, 250, 330, 855 |
| BstV1I | GCAGC | 2 cut(s) | 393, 562 |
| BstV2I | GAAGAC | 2 cut(s) | 200, 243 |
| BstX2I | RGATCY | 1 cut(s) | 433 |
| BstYI | RGATCY | 1 cut(s) | 433 |
| Bsu36I | CCTNAGG | 1 cut(s) | 676 |
| BsuRI | GGCC | 8 cut(s) | 56, 119, 396, 482, 488, 503, 863, 894 |
| BtgI | CCRYGG | 1 cut(s) | 113 |
| BtgZI | GCGATG | 1 cut(s) | 769 |
| BtsCI | GGATG | 1 cut(s) | 787 |
| BtsI | GCAGTG | 3 cut(s) | 96, 651, 877 |
| BtsIMutI | CAGTG | 3 cut(s) | 96, 651, 877 |
| BtuMI | TCGCGA | 1 cut(s) | 855 |
| Cac8I | GCNNGC | 5 cut(s) | 63, 423, 592, 752, 892 |
| CaiI | CAGNNNCTG | 1 cut(s) | 151 |
| CfoI | GCGC | 2 cut(s) | 109, 332 |
| Cfr10I | RCCGGY | 2 cut(s) | 750, 902 |
| Cfr13I | GGNCC | 5 cut(s) | 82, 117, 487, 603, 867 |
| CseI | GACGC | 4 cut(s) | 111, 113, 157, 272 |
| Csp6I | GTAC | 4 cut(s) | 5, 17, 615, 733 |
| CviQI | GTAC | 4 cut(s) | 5, 17, 615, 733 |
| DdeI | CTNAG | 1 cut(s) | 676 |
| DpnI | GATC | 4 cut(s) | 95, 282, 435, 719 |
| DpnII | GATC | 4 cut(s) | 93, 280, 433, 717 |
| DriI | GACNNNNNGTC | 1 cut(s) | 268 |
| EaeI | YGGCCR | 3 cut(s) | 54, 501, 892 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 268 |
| EciI | GGCGGA | 2 cut(s) | 277, 446 |
| Eco130I | CCWWGG | 2 cut(s) | 30, 483 |
| Eco31I | GGTCTC | 1 cut(s) | 258 |
| Eco47I | GGWCC | 3 cut(s) | 82, 603, 867 |
| Eco81I | CCTNAGG | 1 cut(s) | 676 |
| Eco91I | GGTNACC | 1 cut(s) | 37 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 217, 707 |
| EcoO65I | GGTNACC | 1 cut(s) | 37 |
| EcoRII | CCWGG | 8 cut(s) | 56, 78, 373, 391, 708, 735, 858, 863 |
| EcoT14I | CCWWGG | 2 cut(s) | 30, 483 |
| ErhI | CCWWGG | 2 cut(s) | 30, 483 |
| FaiI | YATR | 6 cut(s) | 216, 337, 417, 908, 910, 937 |
| FaqI | GGGAC | 4 cut(s) | 157, 499, 555, 616 |
| FauI | CCCGC | 6 cut(s) | 54, 396, 524, 580, 587, 755 |
| FauNDI | CATATG | 1 cut(s) | 908 |
| Fnu4HI | GCNGC | 4 cut(s) | 110, 120, 382, 576 |
| FokI | GGATG | 1 cut(s) | 794 |
| Fsp4HI | GCNGC | 4 cut(s) | 110, 120, 382, 576 |
| FspBI | CTAG | 3 cut(s) | 11, 591, 741 |
| GlaI | GCGC | 2 cut(s) | 108, 331 |
| GluI | GCNGC | 4 cut(s) | 110, 120, 382, 576 |
| GsaI | CCCAGC | 3 cut(s) | 314, 623, 831 |
| GsuI | CTGGAG | 2 cut(s) | 128, 563 |
| HaeIII | GGCC | 8 cut(s) | 56, 119, 396, 482, 488, 503, 863, 894 |
| HapII | CCGG | 6 cut(s) | 347, 357, 560, 751, 809, 903 |
| HgaI | GACGC | 4 cut(s) | 111, 113, 157, 272 |
| HhaI | GCGC | 2 cut(s) | 109, 332 |
| Hin1I | GRCGYC | 1 cut(s) | 264 |
| Hin6I | GCGC | 2 cut(s) | 107, 330 |
| HinP1I | GCGC | 2 cut(s) | 107, 330 |
| HincII | GTYRAC | 1 cut(s) | 352 |
| HindII | GTYRAC | 1 cut(s) | 352 |
| HinfI | GANTC | 4 cut(s) | 22, 645, 816, 917 |
| HpaII | CCGG | 6 cut(s) | 347, 357, 560, 751, 809, 903 |
| HphI | GGTGA | 3 cut(s) | 202, 374, 654 |
| Hpy166II | GTNNAC | 2 cut(s) | 352, 461 |
| Hpy188I | TCNGA | 4 cut(s) | 138, 182, 262, 900 |
| Hpy188III | TCNNGA | 5 cut(s) | 126, 145, 670, 820, 854 |
| Hpy8I | GTNNAC | 2 cut(s) | 352, 461 |
| HpyAV | CCTTC | 2 cut(s) | 41, 472 |
| HpyCH4V | TGCA | 7 cut(s) | 339, 578, 656, 759, 785, 882, 912 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 62, 139, 148, 327, 336, 756 |
| HpyF3I | CTNAG | 1 cut(s) | 676 |
| Hsp92I | GRCGYC | 1 cut(s) | 264 |
| HspAI | GCGC | 2 cut(s) | 107, 330 |
| KpnI | GGTACC | 2 cut(s) | 8, 20 |
| KroI | GCCGGC | 1 cut(s) | 750 |
| KroNI | GCCGGC | 1 cut(s) | 752 |
| Kzo9I | GATC | 4 cut(s) | 93, 280, 433, 717 |
| LmnI | GCTCC | 1 cut(s) | 147 |
| Lsp1109I | GCAGC | 2 cut(s) | 393, 562 |
| LweI | GCATC | 4 cut(s) | 142, 232, 746, 772 |
| MaeI | CTAG | 3 cut(s) | 11, 591, 741 |
| MaeIII | GTNAC | 3 cut(s) | 37, 631, 928 |
| MalI | GATC | 4 cut(s) | 95, 282, 435, 719 |
| MbiI | CCGCTC | 2 cut(s) | 71, 667 |
| MboI | GATC | 4 cut(s) | 93, 280, 433, 717 |
| MboII | GAAGA | 5 cut(s) | 202, 205, 240, 248, 428 |
| MfeI | CAATTG | 1 cut(s) | 789 |
| MflI | RGATCY | 1 cut(s) | 433 |
| MlsI | TGGCCA | 1 cut(s) | 503 |
| MluCI | AATT | 2 cut(s) | 789, 883 |
| MluNI | TGGCCA | 1 cut(s) | 503 |
| MlyI | GAGTC | 3 cut(s) | 16, 654, 911 |
| Mox20I | TGGCCA | 1 cut(s) | 503 |
| MreI | CGCCGGCG | 1 cut(s) | 750 |
| MroNI | GCCGGC | 1 cut(s) | 750 |
| MscI | TGGCCA | 1 cut(s) | 503 |
| MseI | TTAA | 2 cut(s) | 324, 804 |
| MslI | CAYNNNNRTG | 1 cut(s) | 509 |
| Msp20I | TGGCCA | 1 cut(s) | 503 |
| MspA1I | CMGCKG | 3 cut(s) | 314, 575, 728 |
| MspI | CCGG | 6 cut(s) | 347, 357, 560, 751, 809, 903 |
| MunI | CAATTG | 1 cut(s) | 789 |
| MvaI | CCWGG | 8 cut(s) | 58, 80, 375, 393, 710, 737, 860, 865 |
| MvnI | CGCG | 5 cut(s) | 107, 122, 250, 330, 855 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 62, 139, 148, 327, 336, 756 |
| NaeI | GCCGGC | 1 cut(s) | 752 |
| NciI | CCSGG | 4 cut(s) | 347, 357, 560, 810 |
| NdeI | CATATG | 1 cut(s) | 908 |
| NdeII | GATC | 4 cut(s) | 93, 280, 433, 717 |
| NgoMIV | GCCGGC | 1 cut(s) | 750 |
| NheI | GCTAGC | 1 cut(s) | 590 |
| NlaIV | GGNNCC | 4 cut(s) | 6, 18, 604, 824 |
| NmuCI | GTSAC | 1 cut(s) | 928 |
| NruI | TCGCGA | 1 cut(s) | 855 |
| OliI | CACNNNNGTG | 1 cut(s) | 509 |
| PdiI | GCCGGC | 1 cut(s) | 752 |
| PfeI | GAWTC | 1 cut(s) | 816 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 151 |
| PkrI | GCNGC | 4 cut(s) | 111, 121, 383, 577 |
| PleI | GAGTC | 3 cut(s) | 16, 653, 911 |
| PpsI | GAGTC | 3 cut(s) | 16, 653, 911 |
| Psp6I | CCWGG | 8 cut(s) | 56, 78, 373, 391, 708, 735, 858, 863 |
| PspEI | GGTNACC | 1 cut(s) | 37 |
| PspFI | CCCAGC | 3 cut(s) | 310, 619, 827 |
| PspGI | CCWGG | 8 cut(s) | 56, 78, 373, 391, 708, 735, 858, 863 |
| PspN4I | GGNNCC | 4 cut(s) | 6, 18, 604, 824 |
| PspPI | GGNCC | 5 cut(s) | 82, 117, 487, 603, 867 |
| PstNI | CAGNNNCTG | 1 cut(s) | 151 |
| PsuI | RGATCY | 1 cut(s) | 433 |
| RruI | TCGCGA | 1 cut(s) | 855 |
| RsaI | GTAC | 4 cut(s) | 6, 18, 616, 734 |
| RsaNI | GTAC | 4 cut(s) | 5, 17, 615, 733 |
| RseI | CAYNNNNRTG | 1 cut(s) | 509 |
| SaqAI | TTAA | 2 cut(s) | 324, 804 |
| SatI | GCNGC | 4 cut(s) | 110, 120, 382, 576 |
| Sau3AI | GATC | 4 cut(s) | 93, 280, 433, 717 |
| Sau96I | GGNCC | 5 cut(s) | 82, 117, 487, 603, 867 |
| SchI | GAGTC | 3 cut(s) | 16, 654, 911 |
| SfaNI | GCATC | 4 cut(s) | 142, 232, 746, 772 |
| SgrAI | CRCCGGYG | 1 cut(s) | 750 |
| SinI | GGWCC | 3 cut(s) | 82, 603, 867 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 509 |
| SmlI | CTYRAG | 1 cut(s) | 668 |
| SmoI | CTYRAG | 1 cut(s) | 668 |
| Sse9I | AATT | 2 cut(s) | 789, 883 |
| SspMI | CTAG | 3 cut(s) | 11, 591, 741 |
| StyI | CCWWGG | 2 cut(s) | 30, 483 |
| TaqI | TCGA | 3 cut(s) | 73, 159, 226 |
| TasI | AATT | 2 cut(s) | 789, 883 |
| TauI | GCSGC | 2 cut(s) | 112, 122 |
| TfiI | GAWTC | 1 cut(s) | 816 |
| Tru1I | TTAA | 2 cut(s) | 324, 804 |
| Tru9I | TTAA | 2 cut(s) | 324, 804 |
| TscAI | CASTG | 3 cut(s) | 103, 658, 884 |
| TseFI | GTSAC | 1 cut(s) | 928 |
| TseI | GCWGC | 2 cut(s) | 381, 575 |
| Tsp45I | GTSAC | 1 cut(s) | 928 |
| TspRI | CASTG | 3 cut(s) | 103, 658, 884 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 151 |
| VpaK11BI | GGWCC | 3 cut(s) | 82, 603, 867 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 217, 707 |
| XspI | CTAG | 3 cut(s) | 11, 591, 741 |