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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 304 |
| Acc36I | ACCTGC | 1 cut(s) | 304 |
| AccII | CGCG | 2 cut(s) | 17, 397 |
| AciI | CCGC | 8 cut(s) | 17, 38, 72, 92, 167, 212, 254, 397 |
| AclI | AACGTT | 1 cut(s) | 99 |
| AclWI | GGATC | 2 cut(s) | 48, 231 |
| AcoI | YGGCCR | 2 cut(s) | 228, 318 |
| AcsI | RAATTY | 1 cut(s) | 335 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 139, 265, 313 |
| AjnI | CCWGG | 1 cut(s) | 196 |
| AluBI | AGCT | 3 cut(s) | 47, 77, 244 |
| AluI | AGCT | 3 cut(s) | 47, 77, 244 |
| Alw21I | GWGCWC | 2 cut(s) | 112, 404 |
| AlwI | GGATC | 2 cut(s) | 48, 231 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 226 |
| AoxI | GGCC | 3 cut(s) | 228, 303, 318 |
| ApeKI | GCWGC | 5 cut(s) | 47, 74, 182, 201, 358 |
| ApoI | RAATTY | 1 cut(s) | 335 |
| AspLEI | GCGC | 3 cut(s) | 17, 288, 382 |
| AspS9I | GGNCC | 2 cut(s) | 256, 304 |
| AsuC2I | CCSGG | 1 cut(s) | 216 |
| AsuHPI | GGTGA | 1 cut(s) | 200 |
| AvaII | GGWCC | 1 cut(s) | 256 |
| BalI | TGGCCA | 2 cut(s) | 230, 320 |
| Bbv12I | GWGCWC | 2 cut(s) | 112, 404 |
| BbvI | GCAGC | 5 cut(s) | 34, 86, 194, 213, 345 |
| BccI | CCATC | 2 cut(s) | 66, 379 |
| BciT130I | CCWGG | 1 cut(s) | 198 |
| BcnI | CCSGG | 1 cut(s) | 216 |
| BfaI | CTAG | 1 cut(s) | 135 |
| BfmI | CTRYAG | 2 cut(s) | 180, 359 |
| BfuAI | ACCTGC | 1 cut(s) | 304 |
| BisI | GCNGC | 5 cut(s) | 48, 75, 183, 202, 359 |
| BlsI | GCNGC | 5 cut(s) | 49, 76, 184, 203, 360 |
| Bme1390I | CCNGG | 2 cut(s) | 198, 216 |
| Bme18I | GGWCC | 1 cut(s) | 256 |
| BmgT120I | GGNCC | 2 cut(s) | 256, 304 |
| BmiI | GGNNCC | 1 cut(s) | 144 |
| BmrFI | CCNGG | 2 cut(s) | 198, 216 |
| BpuMI | CCSGG | 1 cut(s) | 216 |
| BsaJI | CCNNGG | 1 cut(s) | 148 |
| BsaWI | WCCGGW | 1 cut(s) | 124 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 139, 265, 313 |
| BseBI | CCWGG | 1 cut(s) | 198 |
| BseDI | CCNNGG | 1 cut(s) | 148 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 139, 265, 313 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseXI | GCAGC | 5 cut(s) | 34, 86, 194, 213, 345 |
| BseYI | CCCAGC | 2 cut(s) | 88, 376 |
| Bsh1236I | CGCG | 2 cut(s) | 17, 397 |
| BshFI | GGCC | 3 cut(s) | 230, 305, 320 |
| BsiHKAI | GWGCWC | 2 cut(s) | 112, 404 |
| BsiSI | CCGG | 2 cut(s) | 125, 215 |
| BslFI | GGGAC | 1 cut(s) | 142 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 139, 265, 313 |
| BsmFI | GGGAC | 1 cut(s) | 142 |
| BsnI | GGCC | 3 cut(s) | 230, 305, 320 |
| Bsp1286I | GDGCHC | 2 cut(s) | 112, 404 |
| Bsp143I | GATC | 2 cut(s) | 40, 223 |
| Bsp19I | CCATGG | 1 cut(s) | 148 |
| BspACI | CCGC | 8 cut(s) | 17, 38, 72, 92, 167, 212, 254, 397 |
| BspANI | GGCC | 3 cut(s) | 230, 305, 320 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspFNI | CGCG | 2 cut(s) | 17, 397 |
| BspLI | GGNNCC | 1 cut(s) | 144 |
| BspMAI | CTGCAG | 2 cut(s) | 184, 363 |
| BspMI | ACCTGC | 1 cut(s) | 304 |
| BspPI | GGATC | 2 cut(s) | 48, 231 |
| BssECI | CCNNGG | 1 cut(s) | 148 |
| BssMI | GATC | 2 cut(s) | 40, 223 |
| BssT1I | CCWWGG | 1 cut(s) | 148 |
| Bst2UI | CCWGG | 1 cut(s) | 198 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 246 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstDSI | CCRYGG | 1 cut(s) | 148 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstFNI | CGCG | 2 cut(s) | 17, 397 |
| BstHHI | GCGC | 3 cut(s) | 17, 288, 382 |
| BstKTI | GATC | 2 cut(s) | 43, 226 |
| BstMBI | GATC | 2 cut(s) | 40, 223 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 44, 377, 388, 408 |
| BstNI | CCWGG | 1 cut(s) | 198 |
| BstSCI | CCNGG | 2 cut(s) | 196, 214 |
| BstSFI | CTRYAG | 2 cut(s) | 180, 359 |
| BstUI | CGCG | 2 cut(s) | 17, 397 |
| BstV1I | GCAGC | 5 cut(s) | 34, 86, 194, 213, 345 |
| BstX2I | RGATCY | 1 cut(s) | 223 |
| BstYI | RGATCY | 1 cut(s) | 223 |
| BsuRI | GGCC | 3 cut(s) | 230, 305, 320 |
| BtgI | CCRYGG | 1 cut(s) | 148 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| BtsIMutI | CAGTG | 1 cut(s) | 24 |
| BveI | ACCTGC | 1 cut(s) | 304 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 246 |
| CaiI | CAGNNNCTG | 1 cut(s) | 226 |
| CfoI | GCGC | 3 cut(s) | 17, 288, 382 |
| Cfr13I | GGNCC | 2 cut(s) | 256, 304 |
| CviAII | CATG | 1 cut(s) | 149 |
| CviJI | RGCY | 7 cut(s) | 47, 77, 153, 230, 244, 305, 320 |
| CviKI_1 | RGCY | 7 cut(s) | 47, 77, 153, 230, 244, 305, 320 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 2 cut(s) | 42, 225 |
| DpnII | GATC | 2 cut(s) | 40, 223 |
| EaeI | YGGCCR | 2 cut(s) | 228, 318 |
| EciI | GGCGGA | 1 cut(s) | 269 |
| Eco130I | CCWWGG | 1 cut(s) | 148 |
| Eco47I | GGWCC | 1 cut(s) | 256 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoRII | CCWGG | 1 cut(s) | 196 |
| EcoT14I | CCWWGG | 1 cut(s) | 148 |
| ErhI | CCWWGG | 1 cut(s) | 148 |
| FaeI | CATG | 1 cut(s) | 152 |
| FaiI | YATR | 3 cut(s) | 150, 236, 240 |
| FaqI | GGGAC | 1 cut(s) | 142 |
| FatI | CATG | 1 cut(s) | 148 |
| FauI | CCCGC | 5 cut(s) | 10, 79, 85, 174, 219 |
| Fnu4HI | GCNGC | 5 cut(s) | 48, 75, 183, 202, 359 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 5 cut(s) | 48, 75, 183, 202, 359 |
| FspBI | CTAG | 1 cut(s) | 135 |
| GlaI | GCGC | 3 cut(s) | 16, 287, 381 |
| GluI | GCNGC | 5 cut(s) | 48, 75, 183, 202, 359 |
| GsaI | CCCAGC | 2 cut(s) | 92, 380 |
| HaeIII | GGCC | 3 cut(s) | 230, 305, 320 |
| HapII | CCGG | 2 cut(s) | 125, 215 |
| HhaI | GCGC | 3 cut(s) | 17, 288, 382 |
| Hin1II | CATG | 1 cut(s) | 152 |
| Hin6I | GCGC | 3 cut(s) | 15, 286, 380 |
| HinP1I | GCGC | 3 cut(s) | 15, 286, 380 |
| HincII | GTYRAC | 2 cut(s) | 103, 130 |
| HindII | GTYRAC | 2 cut(s) | 103, 130 |
| HpaI | GTTAAC | 1 cut(s) | 103 |
| HpaII | CCGG | 2 cut(s) | 125, 215 |
| HphI | GGTGA | 1 cut(s) | 200 |
| Hpy166II | GTNNAC | 2 cut(s) | 103, 130 |
| Hpy188III | TCNNGA | 1 cut(s) | 221 |
| Hpy8I | GTNNAC | 2 cut(s) | 103, 130 |
| HpyAV | CCTTC | 1 cut(s) | 289 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 1 cut(s) | 99 |
| HpyCH4V | TGCA | 2 cut(s) | 182, 361 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 44, 377, 388, 408 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HpySE526I | ACGT | 1 cut(s) | 99 |
| Hsp92II | CATG | 1 cut(s) | 152 |
| HspAI | GCGC | 3 cut(s) | 15, 286, 380 |
| KspAI | GTTAAC | 1 cut(s) | 103 |
| Kzo9I | GATC | 2 cut(s) | 40, 223 |
| LmnI | GCTCC | 3 cut(s) | 31, 388, 399 |
| Lsp1109I | GCAGC | 5 cut(s) | 34, 86, 194, 213, 345 |
| MaeI | CTAG | 1 cut(s) | 135 |
| MaeII | ACGT | 1 cut(s) | 99 |
| MaeIII | GTNAC | 2 cut(s) | 95, 206 |
| MalI | GATC | 2 cut(s) | 42, 225 |
| MboI | GATC | 2 cut(s) | 40, 223 |
| MflI | RGATCY | 1 cut(s) | 223 |
| MhlI | GDGCHC | 2 cut(s) | 112, 404 |
| MlsI | TGGCCA | 2 cut(s) | 230, 320 |
| MluCI | AATT | 1 cut(s) | 335 |
| MluNI | TGGCCA | 2 cut(s) | 230, 320 |
| MnlI | CCTC | 3 cut(s) | 22, 269, 295 |
| Mox20I | TGGCCA | 2 cut(s) | 230, 320 |
| MscI | TGGCCA | 2 cut(s) | 230, 320 |
| MseI | TTAA | 2 cut(s) | 102, 415 |
| MslI | CAYNNNNRTG | 1 cut(s) | 326 |
| Msp20I | TGGCCA | 2 cut(s) | 230, 320 |
| MspA1I | CMGCKG | 2 cut(s) | 92, 169 |
| MspI | CCGG | 2 cut(s) | 125, 215 |
| MspR9I | CCNGG | 2 cut(s) | 198, 216 |
| MvaI | CCWGG | 1 cut(s) | 198 |
| MvnI | CGCG | 2 cut(s) | 17, 397 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 44, 377, 388, 408 |
| NciI | CCSGG | 1 cut(s) | 216 |
| NcoI | CCATGG | 1 cut(s) | 148 |
| NdeII | GATC | 2 cut(s) | 40, 223 |
| NlaIII | CATG | 1 cut(s) | 152 |
| NlaIV | GGNNCC | 1 cut(s) | 144 |
| NmuCI | GTSAC | 1 cut(s) | 206 |
| PaqCI | CACCTGC | 1 cut(s) | 304 |
| PkrI | GCNGC | 5 cut(s) | 49, 76, 184, 203, 360 |
| Psp1406I | AACGTT | 1 cut(s) | 99 |
| Psp6I | CCWGG | 1 cut(s) | 196 |
| PspFI | CCCAGC | 2 cut(s) | 88, 376 |
| PspGI | CCWGG | 1 cut(s) | 196 |
| PspN4I | GGNNCC | 1 cut(s) | 144 |
| PspPI | GGNCC | 2 cut(s) | 256, 304 |
| PstI | CTGCAG | 2 cut(s) | 184, 363 |
| PstNI | CAGNNNCTG | 1 cut(s) | 226 |
| PsuI | RGATCY | 1 cut(s) | 223 |
| RseI | CAYNNNNRTG | 1 cut(s) | 326 |
| SaqAI | TTAA | 2 cut(s) | 102, 415 |
| SatI | GCNGC | 5 cut(s) | 48, 75, 183, 202, 359 |
| Sau3AI | GATC | 2 cut(s) | 40, 223 |
| Sau96I | GGNCC | 2 cut(s) | 256, 304 |
| ScrFI | CCNGG | 2 cut(s) | 198, 216 |
| SduI | GDGCHC | 2 cut(s) | 112, 404 |
| SetI | ASST | 7 cut(s) | 49, 79, 102, 246, 261, 300, 318 |
| SfcI | CTRYAG | 2 cut(s) | 180, 359 |
| SinI | GGWCC | 1 cut(s) | 256 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 326 |
| Sse9I | AATT | 1 cut(s) | 335 |
| SsiI | CCGC | 8 cut(s) | 17, 38, 72, 92, 167, 212, 254, 397 |
| SspMI | CTAG | 1 cut(s) | 135 |
| StyD4I | CCNGG | 2 cut(s) | 196, 214 |
| StyI | CCWWGG | 1 cut(s) | 148 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 1 cut(s) | 102 |
| TasI | AATT | 1 cut(s) | 335 |
| Tru1I | TTAA | 2 cut(s) | 102, 415 |
| Tru9I | TTAA | 2 cut(s) | 102, 415 |
| TscAI | CASTG | 1 cut(s) | 31 |
| TseFI | GTSAC | 1 cut(s) | 206 |
| TseI | GCWGC | 5 cut(s) | 47, 74, 182, 201, 358 |
| Tsp45I | GTSAC | 1 cut(s) | 206 |
| TspRI | CASTG | 1 cut(s) | 31 |
| VpaK11BI | GGWCC | 1 cut(s) | 256 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XapI | RAATTY | 1 cut(s) | 335 |
| XspI | CTAG | 1 cut(s) | 135 |