No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 256 |
| Acc36I | ACCTGC | 1 cut(s) | 256 |
| AccB1I | GGYRCC | 1 cut(s) | 369 |
| AccBSI | CCGCTC | 1 cut(s) | 213 |
| AccII | CGCG | 2 cut(s) | 15, 431 |
| AclWI | GGATC | 5 cut(s) | 271, 427, 493, 627, 640 |
| AcoI | YGGCCR | 1 cut(s) | 284 |
| AcyI | GRCGYC | 2 cut(s) | 370, 558 |
| AdeI | CACNNNGTG | 1 cut(s) | 567 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 175, 217, 260, 293, 412 |
| AgsI | TTSAA | 1 cut(s) | 388 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 368, 400 |
| AluBI | AGCT | 1 cut(s) | 136 |
| AluI | AGCT | 1 cut(s) | 136 |
| AlwI | GGATC | 5 cut(s) | 271, 427, 493, 627, 640 |
| Ama87I | CYCGRG | 2 cut(s) | 75, 214 |
| AoxI | GGCC | 2 cut(s) | 284, 448 |
| ApeKI | GCWGC | 4 cut(s) | 80, 133, 199, 331 |
| AspLEI | GCGC | 1 cut(s) | 372 |
| AspS9I | GGNCC | 4 cut(s) | 115, 268, 416, 448 |
| AsuC2I | CCSGG | 4 cut(s) | 294, 356, 414, 630 |
| AsuHPI | GGTGA | 2 cut(s) | 49, 415 |
| AvaI | CYCGRG | 2 cut(s) | 75, 214 |
| AvaII | GGWCC | 3 cut(s) | 115, 268, 416 |
| BaeGI | GKGCMC | 1 cut(s) | 165 |
| BamHI | GGATCC | 1 cut(s) | 632 |
| BanI | GGYRCC | 1 cut(s) | 369 |
| BanII | GRGCYC | 1 cut(s) | 293 |
| BbvI | GCAGC | 4 cut(s) | 67, 145, 186, 343 |
| BccI | CCATC | 1 cut(s) | 57 |
| BceAI | ACGGC | 2 cut(s) | 438, 501 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 290, 324 |
| BcnI | CCSGG | 4 cut(s) | 294, 356, 414, 630 |
| BfaI | CTAG | 3 cut(s) | 93, 222, 649 |
| BfmI | CTRYAG | 1 cut(s) | 437 |
| BfoI | RGCGCY | 1 cut(s) | 373 |
| BfuAI | ACCTGC | 1 cut(s) | 256 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 297 |
| BisI | GCNGC | 7 cut(s) | 81, 134, 200, 298, 332, 429, 451 |
| BlsI | GCNGC | 7 cut(s) | 82, 135, 201, 299, 333, 430, 452 |
| Bme1390I | CCNGG | 4 cut(s) | 294, 356, 414, 630 |
| Bme18I | GGWCC | 3 cut(s) | 115, 268, 416 |
| BmeT110I | CYCGRG | 2 cut(s) | 75, 214 |
| BmgT120I | GGNCC | 4 cut(s) | 115, 268, 416, 448 |
| BmiI | GGNNCC | 6 cut(s) | 116, 117, 150, 371, 417, 634 |
| BmrFI | CCNGG | 4 cut(s) | 294, 356, 414, 630 |
| BmsI | GCATC | 2 cut(s) | 318, 411 |
| BpuMI | CCSGG | 4 cut(s) | 294, 356, 414, 630 |
| BsaHI | GRCGYC | 2 cut(s) | 370, 558 |
| BsaJI | CCNNGG | 4 cut(s) | 32, 234, 292, 355 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 175, 217, 260, 293, 412 |
| Bse118I | RCCGGY | 1 cut(s) | 609 |
| Bse3DI | GCAATG | 1 cut(s) | 609 |
| BseDI | CCNNGG | 4 cut(s) | 32, 234, 292, 355 |
| BseGI | GGATG | 2 cut(s) | 333, 402 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 175, 217, 260, 293, 412 |
| BseMI | GCAATG | 1 cut(s) | 609 |
| BseRI | GAGGAG | 1 cut(s) | 198 |
| BseSI | GKGCMC | 1 cut(s) | 165 |
| BseXI | GCAGC | 4 cut(s) | 67, 145, 186, 343 |
| BsgI | GTGCAG | 1 cut(s) | 185 |
| Bsh1236I | CGCG | 2 cut(s) | 15, 431 |
| BshFI | GGCC | 2 cut(s) | 286, 450 |
| BshNI | GGYRCC | 1 cut(s) | 369 |
| BsiHKCI | CYCGRG | 2 cut(s) | 75, 214 |
| BsiSI | CCGG | 8 cut(s) | 271, 294, 320, 355, 413, 590, 610, 629 |
| BslFI | GGGAC | 2 cut(s) | 101, 429 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 175, 217, 260, 293, 412 |
| BsmFI | GGGAC | 2 cut(s) | 101, 429 |
| BsmI | GAATGC | 1 cut(s) | 283 |
| BsnI | GGCC | 2 cut(s) | 286, 450 |
| BsoBI | CYCGRG | 2 cut(s) | 75, 214 |
| Bsp1286I | GDGCHC | 2 cut(s) | 165, 293 |
| Bsp143I | GATC | 4 cut(s) | 263, 432, 498, 632 |
| Bsp68I | TCGCGA | 1 cut(s) | 15 |
| BspANI | GGCC | 2 cut(s) | 286, 450 |
| BspFNI | CGCG | 2 cut(s) | 15, 431 |
| BspLI | GGNNCC | 6 cut(s) | 116, 117, 150, 371, 417, 634 |
| BspMI | ACCTGC | 1 cut(s) | 256 |
| BspPI | GGATC | 5 cut(s) | 271, 427, 493, 627, 640 |
| BspQI | GCTCTTC | 1 cut(s) | 620 |
| BspT107I | GGYRCC | 1 cut(s) | 369 |
| BsrBI | CCGCTC | 1 cut(s) | 213 |
| BsrDI | GCAATG | 1 cut(s) | 609 |
| BsrFI | RCCGGY | 1 cut(s) | 609 |
| BssAI | RCCGGY | 1 cut(s) | 609 |
| BssECI | CCNNGG | 4 cut(s) | 32, 234, 292, 355 |
| BssMI | GATC | 4 cut(s) | 263, 432, 498, 632 |
| BssNI | GRCGYC | 2 cut(s) | 370, 558 |
| Bst4CI | ACNGT | 1 cut(s) | 441 |
| Bst6I | CTCTTC | 1 cut(s) | 620 |
| BstACI | GRCGYC | 2 cut(s) | 370, 558 |
| BstC8I | GCNNGC | 2 cut(s) | 13, 626 |
| BstDSI | CCRYGG | 1 cut(s) | 32 |
| BstF5I | GGATG | 2 cut(s) | 333, 402 |
| BstFNI | CGCG | 2 cut(s) | 15, 431 |
| BstH2I | RGCGCY | 1 cut(s) | 373 |
| BstHHI | GCGC | 1 cut(s) | 372 |
| BstKTI | GATC | 4 cut(s) | 266, 435, 501, 635 |
| BstMBI | GATC | 4 cut(s) | 263, 432, 498, 632 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 297 |
| BstSCI | CCNGG | 4 cut(s) | 292, 354, 412, 628 |
| BstSFI | CTRYAG | 1 cut(s) | 437 |
| BstSLI | GKGCMC | 1 cut(s) | 165 |
| BstUI | CGCG | 2 cut(s) | 15, 431 |
| BstV1I | GCAGC | 4 cut(s) | 67, 145, 186, 343 |
| BstX2I | RGATCY | 1 cut(s) | 632 |
| BstYI | RGATCY | 1 cut(s) | 632 |
| BsuRI | GGCC | 2 cut(s) | 286, 450 |
| BtgI | CCRYGG | 1 cut(s) | 32 |
| BtgZI | GCGATG | 3 cut(s) | 20, 30, 180 |
| BtsCI | GGATG | 2 cut(s) | 333, 402 |
| BtuMI | TCGCGA | 1 cut(s) | 15 |
| BveI | ACCTGC | 1 cut(s) | 256 |
| Cac8I | GCNNGC | 2 cut(s) | 13, 626 |
| CfoI | GCGC | 1 cut(s) | 372 |
| Cfr10I | RCCGGY | 1 cut(s) | 609 |
| Cfr13I | GGNCC | 4 cut(s) | 115, 268, 416, 448 |
| CpoI | CGGWCCG | 1 cut(s) | 268 |
| CseI | GACGC | 2 cut(s) | 311, 566 |
| CspI | CGGWCCG | 1 cut(s) | 268 |
| CviAII | CATG | 1 cut(s) | 57 |
| DinI | GGCGCC | 1 cut(s) | 371 |
| DpnI | GATC | 4 cut(s) | 265, 434, 500, 634 |
| DpnII | GATC | 4 cut(s) | 263, 432, 498, 632 |
| DraIII | CACNNNGTG | 1 cut(s) | 567 |
| EaeI | YGGCCR | 1 cut(s) | 284 |
| Eam1104I | CTCTTC | 1 cut(s) | 620 |
| EarI | CTCTTC | 1 cut(s) | 620 |
| EciI | GGCGGA | 1 cut(s) | 289 |
| Eco24I | GRGCYC | 1 cut(s) | 293 |
| Eco47I | GGWCC | 3 cut(s) | 115, 268, 416 |
| Eco88I | CYCGRG | 2 cut(s) | 75, 214 |
| EcoO109I | RGGNCCY | 1 cut(s) | 115 |
| EcoT38I | GRGCYC | 1 cut(s) | 293 |
| EgeI | GGCGCC | 1 cut(s) | 371 |
| EheI | GGCGCC | 1 cut(s) | 371 |
| FaeI | CATG | 1 cut(s) | 60 |
| FaiI | YATR | 4 cut(s) | 58, 458, 512, 514 |
| FaqI | GGGAC | 2 cut(s) | 101, 429 |
| FatI | CATG | 1 cut(s) | 56 |
| FauI | CCCGC | 2 cut(s) | 126, 173 |
| Fnu4HI | GCNGC | 7 cut(s) | 81, 134, 200, 298, 332, 429, 451 |
| FokI | GGATG | 2 cut(s) | 340, 389 |
| FriOI | GRGCYC | 1 cut(s) | 293 |
| Fsp4HI | GCNGC | 7 cut(s) | 81, 134, 200, 298, 332, 429, 451 |
| FspBI | CTAG | 3 cut(s) | 93, 222, 649 |
| GlaI | GCGC | 1 cut(s) | 371 |
| GluI | GCNGC | 7 cut(s) | 81, 134, 200, 298, 332, 429, 451 |
| HaeII | RGCGCY | 1 cut(s) | 373 |
| HaeIII | GGCC | 2 cut(s) | 286, 450 |
| HapII | CCGG | 8 cut(s) | 271, 294, 320, 355, 413, 590, 610, 629 |
| HgaI | GACGC | 2 cut(s) | 311, 566 |
| HhaI | GCGC | 1 cut(s) | 372 |
| Hin1I | GRCGYC | 2 cut(s) | 370, 558 |
| Hin1II | CATG | 1 cut(s) | 60 |
| Hin6I | GCGC | 1 cut(s) | 370 |
| HinP1I | GCGC | 1 cut(s) | 370 |
| HincII | GTYRAC | 1 cut(s) | 534 |
| HindII | GTYRAC | 1 cut(s) | 534 |
| HinfI | GANTC | 4 cut(s) | 191, 465, 549, 641 |
| HpaII | CCGG | 8 cut(s) | 271, 294, 320, 355, 413, 590, 610, 629 |
| HphI | GGTGA | 2 cut(s) | 49, 415 |
| Hpy166II | GTNNAC | 1 cut(s) | 534 |
| Hpy188III | TCNNGA | 1 cut(s) | 14 |
| Hpy8I | GTNNAC | 1 cut(s) | 534 |
| Hpy99I | CGWCG | 2 cut(s) | 305, 448 |
| HpyAV | CCTTC | 2 cut(s) | 513, 522 |
| HpyCH4III | ACNGT | 1 cut(s) | 441 |
| HpyCH4V | TGCA | 3 cut(s) | 202, 331, 460 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 297 |
| Hsp92I | GRCGYC | 2 cut(s) | 370, 558 |
| Hsp92II | CATG | 1 cut(s) | 60 |
| HspAI | GCGC | 1 cut(s) | 370 |
| KasI | GGCGCC | 1 cut(s) | 369 |
| KflI | GGGWCCC | 1 cut(s) | 115 |
| Kzo9I | GATC | 4 cut(s) | 263, 432, 498, 632 |
| LguI | GCTCTTC | 1 cut(s) | 620 |
| LmnI | GCTCC | 2 cut(s) | 148, 178 |
| LpnPI | CCDG | 9 cut(s) | 261, 284, 307, 333, 368, 426, 603, 623, 642 |
| Lsp1109I | GCAGC | 4 cut(s) | 67, 145, 186, 343 |
| LweI | GCATC | 2 cut(s) | 318, 411 |
| MaeI | CTAG | 3 cut(s) | 93, 222, 649 |
| MaeIII | GTNAC | 2 cut(s) | 37, 476 |
| MalI | GATC | 4 cut(s) | 265, 434, 500, 634 |
| MbiI | CCGCTC | 1 cut(s) | 213 |
| MboI | GATC | 4 cut(s) | 263, 432, 498, 632 |
| MboII | GAAGA | 1 cut(s) | 607 |
| MflI | RGATCY | 1 cut(s) | 632 |
| MhlI | GDGCHC | 2 cut(s) | 165, 293 |
| MluCI | AATT | 2 cut(s) | 363, 382 |
| Mly113I | GGCGCC | 1 cut(s) | 370 |
| MlyI | GAGTC | 2 cut(s) | 459, 635 |
| MmeI | TCCRAC | 2 cut(s) | 156, 318 |
| MnlI | CCTC | 7 cut(s) | 13, 176, 179, 210, 229, 254, 592 |
| MseI | TTAA | 2 cut(s) | 350, 524 |
| MslI | CAYNNNNRTG | 2 cut(s) | 61, 407 |
| MspI | CCGG | 8 cut(s) | 271, 294, 320, 355, 413, 590, 610, 629 |
| MspR9I | CCNGG | 4 cut(s) | 294, 356, 414, 630 |
| Mva1269I | GAATGC | 1 cut(s) | 283 |
| MvnI | CGCG | 2 cut(s) | 15, 431 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 297 |
| NarI | GGCGCC | 1 cut(s) | 370 |
| NciI | CCSGG | 4 cut(s) | 294, 356, 414, 630 |
| NdeII | GATC | 4 cut(s) | 263, 432, 498, 632 |
| NlaIII | CATG | 1 cut(s) | 60 |
| NlaIV | GGNNCC | 6 cut(s) | 116, 117, 150, 371, 417, 634 |
| NmeAIII | GCCGAG | 1 cut(s) | 312 |
| NmuCI | GTSAC | 2 cut(s) | 37, 476 |
| NruI | TCGCGA | 1 cut(s) | 15 |
| PaeR7I | CTCGAG | 1 cut(s) | 214 |
| PaqCI | CACCTGC | 1 cut(s) | 256 |
| PciSI | GCTCTTC | 1 cut(s) | 620 |
| PctI | GAATGC | 1 cut(s) | 283 |
| PfeI | GAWTC | 2 cut(s) | 191, 549 |
| PkrI | GCNGC | 7 cut(s) | 82, 135, 201, 299, 333, 430, 452 |
| PleI | GAGTC | 2 cut(s) | 459, 635 |
| PluTI | GGCGCC | 1 cut(s) | 373 |
| PpsI | GAGTC | 2 cut(s) | 459, 635 |
| PpuMI | RGGWCCY | 1 cut(s) | 115 |
| Psp5II | RGGWCCY | 1 cut(s) | 115 |
| PspN4I | GGNNCC | 6 cut(s) | 116, 117, 150, 371, 417, 634 |
| PspPI | GGNCC | 4 cut(s) | 115, 268, 416, 448 |
| PspPPI | RGGWCCY | 1 cut(s) | 115 |
| PspXI | VCTCGAGB | 1 cut(s) | 214 |
| PsuI | RGATCY | 1 cut(s) | 632 |
| RruI | TCGCGA | 1 cut(s) | 15 |
| RseI | CAYNNNNRTG | 2 cut(s) | 61, 407 |
| Rsr2I | CGGWCCG | 1 cut(s) | 268 |
| RsrII | CGGWCCG | 1 cut(s) | 268 |
| SapI | GCTCTTC | 1 cut(s) | 620 |
| SaqAI | TTAA | 2 cut(s) | 350, 524 |
| SatI | GCNGC | 7 cut(s) | 81, 134, 200, 298, 332, 429, 451 |
| Sau3AI | GATC | 4 cut(s) | 263, 432, 498, 632 |
| Sau96I | GGNCC | 4 cut(s) | 115, 268, 416, 448 |
| SchI | GAGTC | 2 cut(s) | 459, 635 |
| ScrFI | CCNGG | 4 cut(s) | 294, 356, 414, 630 |
| SduI | GDGCHC | 2 cut(s) | 165, 293 |
| SetI | ASST | 7 cut(s) | 24, 138, 250, 350, 523, 533, 566 |
| SfaNI | GCATC | 2 cut(s) | 318, 411 |
| SfcI | CTRYAG | 1 cut(s) | 437 |
| SfoI | GGCGCC | 1 cut(s) | 371 |
| Sfr274I | CTCGAG | 1 cut(s) | 214 |
| SinI | GGWCC | 3 cut(s) | 115, 268, 416 |
| SlaI | CTCGAG | 1 cut(s) | 214 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 61, 407 |
| SmlI | CTYRAG | 1 cut(s) | 214 |
| SmoI | CTYRAG | 1 cut(s) | 214 |
| Sse9I | AATT | 2 cut(s) | 363, 382 |
| SspDI | GGCGCC | 1 cut(s) | 369 |
| SspMI | CTAG | 3 cut(s) | 93, 222, 649 |
| StyD4I | CCNGG | 4 cut(s) | 292, 354, 412, 628 |
| TaaI | ACNGT | 1 cut(s) | 441 |
| TaqI | TCGA | 3 cut(s) | 215, 552, 618 |
| TaqII | GACCGA | 2 cut(s) | 256, 511 |
| TasI | AATT | 2 cut(s) | 363, 382 |
| TauI | GCSGC | 3 cut(s) | 300, 431, 453 |
| TfiI | GAWTC | 2 cut(s) | 191, 549 |
| Tru1I | TTAA | 2 cut(s) | 350, 524 |
| Tru9I | TTAA | 2 cut(s) | 350, 524 |
| TseFI | GTSAC | 2 cut(s) | 37, 476 |
| TseI | GCWGC | 4 cut(s) | 80, 133, 199, 331 |
| Tsp45I | GTSAC | 2 cut(s) | 37, 476 |
| TspDTI | ATGAA | 1 cut(s) | 620 |
| TspGWI | ACGGA | 2 cut(s) | 21, 395 |
| VpaK11BI | GGWCC | 3 cut(s) | 115, 268, 416 |
| XhoI | CTCGAG | 1 cut(s) | 214 |
| XspI | CTAG | 3 cut(s) | 93, 222, 649 |