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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 305 |
| AciI | CCGC | 4 cut(s) | 66, 82, 92, 115 |
| AclI | AACGTT | 1 cut(s) | 225 |
| AclWI | GGATC | 1 cut(s) | 38 |
| AcoI | YGGCCR | 1 cut(s) | 208 |
| AfaI | GTAC | 1 cut(s) | 293 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 20, 98, 149, 203 |
| AluBI | AGCT | 1 cut(s) | 128 |
| AluI | AGCT | 1 cut(s) | 128 |
| AlwI | GGATC | 1 cut(s) | 38 |
| AoxI | GGCC | 3 cut(s) | 187, 193, 208 |
| ApeKI | GCWGC | 1 cut(s) | 85 |
| AspLEI | GCGC | 2 cut(s) | 248, 272 |
| AspS9I | GGNCC | 2 cut(s) | 101, 194 |
| AsuHPI | GGTGA | 1 cut(s) | 104 |
| AsuNHI | GCTAGC | 1 cut(s) | 128 |
| AvaII | GGWCC | 1 cut(s) | 101 |
| BalI | TGGCCA | 1 cut(s) | 210 |
| BbvI | GCAGC | 1 cut(s) | 97 |
| BclI | TGATCA | 1 cut(s) | 7 |
| BfaI | CTAG | 3 cut(s) | 16, 129, 295 |
| BfoI | RGCGCY | 1 cut(s) | 249 |
| BisI | GCNGC | 2 cut(s) | 83, 86 |
| BlsI | GCNGC | 2 cut(s) | 84, 87 |
| BmcAI | AGTACT | 1 cut(s) | 293 |
| Bme18I | GGWCC | 1 cut(s) | 101 |
| BmgT120I | GGNCC | 2 cut(s) | 101, 194 |
| BmiI | GGNNCC | 1 cut(s) | 25 |
| BmtI | GCTAGC | 1 cut(s) | 132 |
| BpmI | CTGGAG | 1 cut(s) | 270 |
| BsaJI | CCNNGG | 1 cut(s) | 190 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 20, 98, 149, 203 |
| BseDI | CCNNGG | 1 cut(s) | 190 |
| BseGI | GGATG | 1 cut(s) | 74 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 20, 98, 149, 203 |
| BseXI | GCAGC | 1 cut(s) | 97 |
| BseYI | CCCAGC | 1 cut(s) | 266 |
| BshFI | GGCC | 3 cut(s) | 189, 195, 210 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 20, 98, 149, 203 |
| BsnI | GGCC | 3 cut(s) | 189, 195, 210 |
| Bsp143I | GATC | 2 cut(s) | 7, 43 |
| BspACI | CCGC | 4 cut(s) | 66, 82, 92, 115 |
| BspANI | GGCC | 3 cut(s) | 189, 195, 210 |
| BspLI | GGNNCC | 1 cut(s) | 25 |
| BspOI | GCTAGC | 1 cut(s) | 132 |
| BspPI | GGATC | 1 cut(s) | 38 |
| BssECI | CCNNGG | 1 cut(s) | 190 |
| BssMI | GATC | 2 cut(s) | 7, 43 |
| BstC8I | GCNNGC | 2 cut(s) | 130, 168 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 147 |
| BstF5I | GGATG | 1 cut(s) | 74 |
| BstH2I | RGCGCY | 1 cut(s) | 249 |
| BstHHI | GCGC | 2 cut(s) | 248, 272 |
| BstKTI | GATC | 2 cut(s) | 10, 46 |
| BstMBI | GATC | 2 cut(s) | 7, 43 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 91, 267 |
| BstV1I | GCAGC | 1 cut(s) | 97 |
| BstX2I | RGATCY | 1 cut(s) | 43 |
| BstYI | RGATCY | 1 cut(s) | 43 |
| BsuRI | GGCC | 3 cut(s) | 189, 195, 210 |
| BtsCI | GGATG | 1 cut(s) | 74 |
| BtsIMutI | CAGTG | 1 cut(s) | 132 |
| Cac8I | GCNNGC | 2 cut(s) | 130, 168 |
| CfoI | GCGC | 2 cut(s) | 248, 272 |
| Cfr13I | GGNCC | 2 cut(s) | 101, 194 |
| Csp6I | GTAC | 1 cut(s) | 292 |
| CviJI | RGCY | 8 cut(s) | 26, 33, 72, 88, 128, 189, 195, 210 |
| CviKI_1 | RGCY | 8 cut(s) | 26, 33, 72, 88, 128, 189, 195, 210 |
| CviQI | GTAC | 1 cut(s) | 292 |
| DpnI | GATC | 2 cut(s) | 9, 45 |
| DpnII | GATC | 2 cut(s) | 7, 43 |
| EaeI | YGGCCR | 1 cut(s) | 208 |
| Eco47I | GGWCC | 1 cut(s) | 101 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 147 |
| FaiI | YATR | 3 cut(s) | 52, 120, 124 |
| FbaI | TGATCA | 1 cut(s) | 7 |
| FblI | GTMKAC | 1 cut(s) | 305 |
| Fnu4HI | GCNGC | 2 cut(s) | 83, 86 |
| FokI | GGATG | 1 cut(s) | 61 |
| Fsp4HI | GCNGC | 2 cut(s) | 83, 86 |
| FspBI | CTAG | 3 cut(s) | 16, 129, 295 |
| GlaI | GCGC | 2 cut(s) | 247, 271 |
| GluI | GCNGC | 2 cut(s) | 83, 86 |
| GsaI | CCCAGC | 1 cut(s) | 270 |
| GsuI | CTGGAG | 1 cut(s) | 270 |
| HaeII | RGCGCY | 1 cut(s) | 249 |
| HaeIII | GGCC | 3 cut(s) | 189, 195, 210 |
| HhaI | GCGC | 2 cut(s) | 248, 272 |
| Hin6I | GCGC | 2 cut(s) | 246, 270 |
| HinP1I | GCGC | 2 cut(s) | 246, 270 |
| HincII | GTYRAC | 2 cut(s) | 223, 306 |
| HindII | GTYRAC | 2 cut(s) | 223, 306 |
| HphI | GGTGA | 1 cut(s) | 104 |
| Hpy166II | GTNNAC | 2 cut(s) | 223, 306 |
| Hpy188I | TCNGA | 1 cut(s) | 7 |
| Hpy8I | GTNNAC | 2 cut(s) | 223, 306 |
| HpyAV | CCTTC | 2 cut(s) | 179, 236 |
| HpyCH4IV | ACGT | 3 cut(s) | 213, 225, 299 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 91, 267 |
| HpySE526I | ACGT | 3 cut(s) | 213, 225, 299 |
| HspAI | GCGC | 2 cut(s) | 246, 270 |
| Ksp22I | TGATCA | 1 cut(s) | 7 |
| Kzo9I | GATC | 2 cut(s) | 7, 43 |
| LmnI | GCTCC | 1 cut(s) | 23 |
| LpnPI | CCDG | 7 cut(s) | 15, 54, 143, 164, 189, 234, 280 |
| Lsp1109I | GCAGC | 1 cut(s) | 97 |
| MaeI | CTAG | 3 cut(s) | 16, 129, 295 |
| MaeII | ACGT | 3 cut(s) | 213, 225, 299 |
| MalI | GATC | 2 cut(s) | 9, 45 |
| MboI | GATC | 2 cut(s) | 7, 43 |
| MflI | RGATCY | 1 cut(s) | 43 |
| MlsI | TGGCCA | 1 cut(s) | 210 |
| MluCI | AATT | 1 cut(s) | 234 |
| MluNI | TGGCCA | 1 cut(s) | 210 |
| MnlI | CCTC | 5 cut(s) | 62, 120, 153, 185, 245 |
| Mox20I | TGGCCA | 1 cut(s) | 210 |
| MscI | TGGCCA | 1 cut(s) | 210 |
| MseI | TTAA | 2 cut(s) | 216, 237 |
| MslI | CAYNNNNRTG | 2 cut(s) | 117, 216 |
| Msp20I | TGGCCA | 1 cut(s) | 210 |
| MspA1I | CMGCKG | 1 cut(s) | 66 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 91, 267 |
| NdeII | GATC | 2 cut(s) | 7, 43 |
| NheI | GCTAGC | 1 cut(s) | 128 |
| NlaIV | GGNNCC | 1 cut(s) | 25 |
| NmeAIII | GCCGAG | 1 cut(s) | 215 |
| PflFI | GACNNNGTC | 1 cut(s) | 301 |
| PkrI | GCNGC | 2 cut(s) | 84, 87 |
| Psp1406I | AACGTT | 1 cut(s) | 225 |
| PspFI | CCCAGC | 1 cut(s) | 266 |
| PspN4I | GGNNCC | 1 cut(s) | 25 |
| PspPI | GGNCC | 2 cut(s) | 101, 194 |
| PsuI | RGATCY | 1 cut(s) | 43 |
| PsyI | GACNNNGTC | 1 cut(s) | 301 |
| RsaI | GTAC | 1 cut(s) | 293 |
| RsaNI | GTAC | 1 cut(s) | 292 |
| RseI | CAYNNNNRTG | 2 cut(s) | 117, 216 |
| SalI | GTCGAC | 1 cut(s) | 304 |
| SaqAI | TTAA | 2 cut(s) | 216, 237 |
| SatI | GCNGC | 2 cut(s) | 83, 86 |
| Sau3AI | GATC | 2 cut(s) | 7, 43 |
| Sau96I | GGNCC | 2 cut(s) | 101, 194 |
| ScaI | AGTACT | 1 cut(s) | 293 |
| SetI | ASST | 8 cut(s) | 112, 130, 145, 208, 216, 228, 256, 302 |
| SinI | GGWCC | 1 cut(s) | 101 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 117, 216 |
| Sse9I | AATT | 1 cut(s) | 234 |
| SsiI | CCGC | 4 cut(s) | 66, 82, 92, 115 |
| SspMI | CTAG | 3 cut(s) | 16, 129, 295 |
| TaiI | ACGT | 3 cut(s) | 216, 228, 302 |
| TaqI | TCGA | 1 cut(s) | 305 |
| TasI | AATT | 1 cut(s) | 234 |
| TatI | WGTACW | 1 cut(s) | 291 |
| TauI | GCSGC | 1 cut(s) | 85 |
| Tru1I | TTAA | 2 cut(s) | 216, 237 |
| Tru9I | TTAA | 2 cut(s) | 216, 237 |
| TscAI | CASTG | 1 cut(s) | 139 |
| TseI | GCWGC | 1 cut(s) | 85 |
| TspRI | CASTG | 1 cut(s) | 139 |
| Tth111I | GACNNNGTC | 1 cut(s) | 301 |
| VpaK11BI | GGWCC | 1 cut(s) | 101 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 147 |
| XmiI | GTMKAC | 1 cut(s) | 305 |
| XspI | CTAG | 3 cut(s) | 16, 129, 295 |
| ZrmI | AGTACT | 1 cut(s) | 293 |