No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 29 |
| AccB1I | GGYRCC | 1 cut(s) | 29 |
| AccBSI | CCGCTC | 1 cut(s) | 629 |
| AccII | CGCG | 3 cut(s) | 116, 327, 448 |
| AclI | AACGTT | 1 cut(s) | 123 |
| AclWI | GGATC | 2 cut(s) | 659, 672 |
| AcoI | YGGCCR | 1 cut(s) | 369 |
| AcsI | RAATTY | 1 cut(s) | 386 |
| AcuI | CTGAAG | 1 cut(s) | 491 |
| AdeI | CACNNNGTG | 1 cut(s) | 310 |
| AfaI | GTAC | 1 cut(s) | 31 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 115, 181, 494, 495, 522, 596, 646 |
| AgsI | TTSAA | 1 cut(s) | 160 |
| AjnI | CCWGG | 8 cut(s) | 189, 201, 238, 256, 261, 639, 644, 660 |
| AluBI | AGCT | 2 cut(s) | 17, 286 |
| AluI | AGCT | 2 cut(s) | 17, 286 |
| Alw21I | GWGCWC | 1 cut(s) | 455 |
| AlwI | GGATC | 2 cut(s) | 659, 672 |
| AoxI | GGCC | 6 cut(s) | 231, 259, 348, 354, 369, 642 |
| ApeKI | GCWGC | 2 cut(s) | 224, 243 |
| ApoI | RAATTY | 1 cut(s) | 386 |
| Asp718I | GGTACC | 1 cut(s) | 29 |
| AspLEI | GCGC | 3 cut(s) | 327, 433, 614 |
| AspS9I | GGNCC | 4 cut(s) | 205, 232, 355, 526 |
| AsuC2I | CCSGG | 2 cut(s) | 93, 591 |
| AsuHPI | GGTGA | 1 cut(s) | 596 |
| AvaII | GGWCC | 2 cut(s) | 205, 526 |
| BalI | TGGCCA | 1 cut(s) | 371 |
| BamHI | GGATCC | 1 cut(s) | 664 |
| BanI | GGYRCC | 1 cut(s) | 29 |
| BanII | GRGCYC | 2 cut(s) | 92, 197 |
| Bbv12I | GWGCWC | 1 cut(s) | 455 |
| BbvI | GCAGC | 2 cut(s) | 236, 255 |
| BccI | CCATC | 1 cut(s) | 453 |
| BceAI | ACGGC | 1 cut(s) | 660 |
| BciT130I | CCWGG | 8 cut(s) | 191, 203, 240, 258, 263, 641, 646, 662 |
| BcnI | CCSGG | 2 cut(s) | 93, 591 |
| BfaI | CTAG | 4 cut(s) | 177, 428, 464, 533 |
| BfmI | CTRYAG | 2 cut(s) | 18, 222 |
| BfoI | RGCGCY | 1 cut(s) | 615 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 75, 258 |
| BglII | AGATCT | 1 cut(s) | 298 |
| BisI | GCNGC | 3 cut(s) | 117, 225, 244 |
| BlsI | GCNGC | 3 cut(s) | 118, 226, 245 |
| Bme18I | GGWCC | 2 cut(s) | 205, 526 |
| BmgT120I | GGNCC | 4 cut(s) | 205, 232, 355, 526 |
| BmiI | GGNNCC | 7 cut(s) | 31, 89, 207, 500, 514, 527, 666 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 27 |
| BpmI | CTGGAG | 2 cut(s) | 150, 431 |
| BpuMI | CCSGG | 2 cut(s) | 93, 591 |
| BsaJI | CCNNGG | 7 cut(s) | 91, 114, 189, 190, 201, 351, 645 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 115, 181, 494, 495, 522, 596, 646 |
| Bse118I | RCCGGY | 1 cut(s) | 501 |
| Bse1I | ACTGG | 3 cut(s) | 24, 167, 414 |
| BseBI | CCWGG | 8 cut(s) | 191, 203, 240, 258, 263, 641, 646, 662 |
| BseDI | CCNNGG | 7 cut(s) | 91, 114, 189, 190, 201, 351, 645 |
| BseGI | GGATG | 2 cut(s) | 197, 477 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 115, 181, 494, 495, 522, 596, 646 |
| BseNI | ACTGG | 3 cut(s) | 24, 167, 414 |
| BseRI | GAGGAG | 1 cut(s) | 609 |
| BseXI | GCAGC | 2 cut(s) | 236, 255 |
| BseYI | CCCAGC | 2 cut(s) | 130, 608 |
| BsgI | GTGCAG | 1 cut(s) | 526 |
| Bsh1236I | CGCG | 3 cut(s) | 116, 327, 448 |
| BshFI | GGCC | 6 cut(s) | 233, 261, 350, 356, 371, 644 |
| BshNI | GGYRCC | 1 cut(s) | 29 |
| BsiHKAI | GWGCWC | 1 cut(s) | 455 |
| BsiSI | CCGG | 4 cut(s) | 85, 93, 502, 590 |
| BslFI | GGGAC | 2 cut(s) | 191, 539 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 115, 181, 494, 495, 522, 596, 646 |
| BsmFI | GGGAC | 2 cut(s) | 191, 539 |
| BsnI | GGCC | 6 cut(s) | 233, 261, 350, 356, 371, 644 |
| Bsp1286I | GDGCHC | 3 cut(s) | 92, 197, 455 |
| Bsp143I | GATC | 2 cut(s) | 298, 664 |
| BspANI | GGCC | 6 cut(s) | 233, 261, 350, 356, 371, 644 |
| BspFNI | CGCG | 3 cut(s) | 116, 327, 448 |
| BspLI | GGNNCC | 7 cut(s) | 31, 89, 207, 500, 514, 527, 666 |
| BspMAI | CTGCAG | 1 cut(s) | 226 |
| BspPI | GGATC | 2 cut(s) | 659, 672 |
| BspT107I | GGYRCC | 1 cut(s) | 29 |
| BsrBI | CCGCTC | 1 cut(s) | 629 |
| BsrFI | RCCGGY | 1 cut(s) | 501 |
| BsrI | ACTGG | 3 cut(s) | 24, 167, 414 |
| BssAI | RCCGGY | 1 cut(s) | 501 |
| BssECI | CCNNGG | 7 cut(s) | 91, 114, 189, 190, 201, 351, 645 |
| BssMI | GATC | 2 cut(s) | 298, 664 |
| Bst2UI | CCWGG | 8 cut(s) | 191, 203, 240, 258, 263, 641, 646, 662 |
| Bst4CI | ACNGT | 1 cut(s) | 149 |
| BstC8I | GCNNGC | 5 cut(s) | 76, 288, 329, 440, 673 |
| BstDSI | CCRYGG | 1 cut(s) | 114 |
| BstF5I | GGATG | 2 cut(s) | 197, 477 |
| BstFNI | CGCG | 3 cut(s) | 116, 327, 448 |
| BstH2I | RGCGCY | 1 cut(s) | 615 |
| BstHHI | GCGC | 3 cut(s) | 327, 433, 614 |
| BstKTI | GATC | 2 cut(s) | 301, 667 |
| BstMBI | GATC | 2 cut(s) | 298, 664 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 75, 230, 249, 258, 413, 428, 439 |
| BstNI | CCWGG | 8 cut(s) | 191, 203, 240, 258, 263, 641, 646, 662 |
| BstNSI | RCATGY | 1 cut(s) | 693 |
| BstSFI | CTRYAG | 2 cut(s) | 18, 222 |
| BstUI | CGCG | 3 cut(s) | 116, 327, 448 |
| BstV1I | GCAGC | 2 cut(s) | 236, 255 |
| BstX2I | RGATCY | 2 cut(s) | 298, 664 |
| BstYI | RGATCY | 2 cut(s) | 298, 664 |
| BsuRI | GGCC | 6 cut(s) | 233, 261, 350, 356, 371, 644 |
| BtgI | CCRYGG | 1 cut(s) | 114 |
| BtsCI | GGATG | 2 cut(s) | 197, 477 |
| BtsI | GCAGTG | 2 cut(s) | 514, 655 |
| BtsIMutI | CAGTG | 4 cut(s) | 31, 407, 514, 655 |
| Cac8I | GCNNGC | 5 cut(s) | 76, 288, 329, 440, 673 |
| CfoI | GCGC | 3 cut(s) | 327, 433, 614 |
| Cfr10I | RCCGGY | 1 cut(s) | 501 |
| Cfr13I | GGNCC | 4 cut(s) | 205, 232, 355, 526 |
| Cfr42I | CCGCGG | 1 cut(s) | 117 |
| CseI | GACGC | 1 cut(s) | 625 |
| Csp6I | GTAC | 1 cut(s) | 30 |
| CviAII | CATG | 2 cut(s) | 690, 725 |
| CviQI | GTAC | 1 cut(s) | 30 |
| DpnI | GATC | 2 cut(s) | 300, 666 |
| DpnII | GATC | 2 cut(s) | 298, 664 |
| DraIII | CACNNNGTG | 1 cut(s) | 310 |
| EaeI | YGGCCR | 1 cut(s) | 369 |
| EciI | GGCGGA | 1 cut(s) | 470 |
| Eco24I | GRGCYC | 2 cut(s) | 92, 197 |
| Eco47I | GGWCC | 2 cut(s) | 205, 526 |
| Eco57I | CTGAAG | 1 cut(s) | 491 |
| EcoO109I | RGGNCCY | 1 cut(s) | 205 |
| EcoRII | CCWGG | 8 cut(s) | 189, 201, 238, 256, 261, 639, 644, 660 |
| EcoT38I | GRGCYC | 2 cut(s) | 92, 197 |
| FaeI | CATG | 2 cut(s) | 693, 728 |
| FaiI | YATR | 4 cut(s) | 278, 282, 691, 726 |
| FaqI | GGGAC | 2 cut(s) | 191, 539 |
| FatI | CATG | 2 cut(s) | 689, 724 |
| FauI | CCCGC | 3 cut(s) | 121, 216, 516 |
| Fnu4HI | GCNGC | 3 cut(s) | 117, 225, 244 |
| FokI | GGATG | 2 cut(s) | 184, 464 |
| FriOI | GRGCYC | 2 cut(s) | 92, 197 |
| Fsp4HI | GCNGC | 3 cut(s) | 117, 225, 244 |
| FspBI | CTAG | 4 cut(s) | 177, 428, 464, 533 |
| GlaI | GCGC | 3 cut(s) | 326, 432, 613 |
| GluI | GCNGC | 3 cut(s) | 117, 225, 244 |
| GsaI | CCCAGC | 2 cut(s) | 134, 612 |
| GsuI | CTGGAG | 2 cut(s) | 150, 431 |
| HaeII | RGCGCY | 1 cut(s) | 615 |
| HaeIII | GGCC | 6 cut(s) | 233, 261, 350, 356, 371, 644 |
| HapII | CCGG | 4 cut(s) | 85, 93, 502, 590 |
| HgaI | GACGC | 1 cut(s) | 625 |
| HhaI | GCGC | 3 cut(s) | 327, 433, 614 |
| Hin1II | CATG | 2 cut(s) | 693, 728 |
| Hin6I | GCGC | 3 cut(s) | 325, 431, 612 |
| HinP1I | GCGC | 3 cut(s) | 325, 431, 612 |
| HincII | GTYRAC | 3 cut(s) | 127, 145, 172 |
| HindII | GTYRAC | 3 cut(s) | 127, 145, 172 |
| HinfI | GANTC | 2 cut(s) | 185, 698 |
| HpaI | GTTAAC | 2 cut(s) | 127, 145 |
| HpaII | CCGG | 4 cut(s) | 85, 93, 502, 590 |
| HphI | GGTGA | 1 cut(s) | 596 |
| Hpy166II | GTNNAC | 3 cut(s) | 127, 145, 172 |
| Hpy188I | TCNGA | 1 cut(s) | 681 |
| Hpy8I | GTNNAC | 3 cut(s) | 127, 145, 172 |
| Hpy99I | CGWCG | 2 cut(s) | 395, 723 |
| HpyAV | CCTTC | 2 cut(s) | 466, 553 |
| HpyCH4III | ACNGT | 1 cut(s) | 149 |
| HpyCH4IV | ACGT | 1 cut(s) | 123 |
| HpyCH4V | TGCA | 6 cut(s) | 152, 224, 507, 566, 671, 693 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 75, 230, 249, 258, 413, 428, 439 |
| HpySE526I | ACGT | 1 cut(s) | 123 |
| Hsp92II | CATG | 2 cut(s) | 693, 728 |
| HspAI | GCGC | 3 cut(s) | 325, 431, 612 |
| KpnI | GGTACC | 1 cut(s) | 33 |
| KspAI | GTTAAC | 2 cut(s) | 127, 145 |
| KspI | CCGCGG | 1 cut(s) | 117 |
| Kzo9I | GATC | 2 cut(s) | 298, 664 |
| LmnI | GCTCC | 4 cut(s) | 87, 322, 450, 498 |
| Lsp1109I | GCAGC | 2 cut(s) | 236, 255 |
| MaeI | CTAG | 4 cut(s) | 177, 428, 464, 533 |
| MaeII | ACGT | 1 cut(s) | 123 |
| MaeIII | GTNAC | 2 cut(s) | 50, 602 |
| MalI | GATC | 2 cut(s) | 300, 666 |
| MbiI | CCGCTC | 1 cut(s) | 629 |
| MboI | GATC | 2 cut(s) | 298, 664 |
| MboII | GAAGA | 1 cut(s) | 293 |
| MflI | RGATCY | 2 cut(s) | 298, 664 |
| MhlI | GDGCHC | 3 cut(s) | 92, 197, 455 |
| MlsI | TGGCCA | 1 cut(s) | 371 |
| MluCI | AATT | 3 cut(s) | 386, 570, 616 |
| MluNI | TGGCCA | 1 cut(s) | 371 |
| MlyI | GAGTC | 1 cut(s) | 692 |
| MnlI | CCTC | 7 cut(s) | 223, 346, 406, 488, 526, 587, 590 |
| Mox20I | TGGCCA | 1 cut(s) | 371 |
| MscI | TGGCCA | 1 cut(s) | 371 |
| MseI | TTAA | 3 cut(s) | 126, 138, 144 |
| MslI | CAYNNNNRTG | 1 cut(s) | 377 |
| Msp20I | TGGCCA | 1 cut(s) | 371 |
| MspA1I | CMGCKG | 4 cut(s) | 116, 134, 211, 227 |
| MspI | CCGG | 4 cut(s) | 85, 93, 502, 590 |
| MvaI | CCWGG | 8 cut(s) | 191, 203, 240, 258, 263, 641, 646, 662 |
| MvnI | CGCG | 3 cut(s) | 116, 327, 448 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 75, 230, 249, 258, 413, 428, 439 |
| NciI | CCSGG | 2 cut(s) | 93, 591 |
| NdeII | GATC | 2 cut(s) | 298, 664 |
| NlaIII | CATG | 2 cut(s) | 693, 728 |
| NlaIV | GGNNCC | 7 cut(s) | 31, 89, 207, 500, 514, 527, 666 |
| NmeAIII | GCCGAG | 1 cut(s) | 376 |
| NmuCI | GTSAC | 2 cut(s) | 50, 602 |
| NspI | RCATGY | 1 cut(s) | 693 |
| PasI | CCCWGGG | 1 cut(s) | 190 |
| PfeI | GAWTC | 1 cut(s) | 185 |
| PfoI | TCCNGGA | 1 cut(s) | 589 |
| PkrI | GCNGC | 3 cut(s) | 118, 226, 245 |
| PleI | GAGTC | 1 cut(s) | 692 |
| PpsI | GAGTC | 1 cut(s) | 692 |
| PpuMI | RGGWCCY | 1 cut(s) | 205 |
| Psp1406I | AACGTT | 1 cut(s) | 123 |
| Psp5II | RGGWCCY | 1 cut(s) | 205 |
| Psp6I | CCWGG | 8 cut(s) | 189, 201, 238, 256, 261, 639, 644, 660 |
| PspFI | CCCAGC | 2 cut(s) | 130, 608 |
| PspGI | CCWGG | 8 cut(s) | 189, 201, 238, 256, 261, 639, 644, 660 |
| PspN4I | GGNNCC | 7 cut(s) | 31, 89, 207, 500, 514, 527, 666 |
| PspPI | GGNCC | 4 cut(s) | 205, 232, 355, 526 |
| PspPPI | RGGWCCY | 1 cut(s) | 205 |
| PstI | CTGCAG | 1 cut(s) | 226 |
| PsuI | RGATCY | 2 cut(s) | 298, 664 |
| RsaI | GTAC | 1 cut(s) | 31 |
| RsaNI | GTAC | 1 cut(s) | 30 |
| RseI | CAYNNNNRTG | 1 cut(s) | 377 |
| SacII | CCGCGG | 1 cut(s) | 117 |
| SaqAI | TTAA | 3 cut(s) | 126, 138, 144 |
| SatI | GCNGC | 3 cut(s) | 117, 225, 244 |
| Sau3AI | GATC | 2 cut(s) | 298, 664 |
| Sau96I | GGNCC | 4 cut(s) | 205, 232, 355, 526 |
| SchI | GAGTC | 1 cut(s) | 692 |
| SduI | GDGCHC | 3 cut(s) | 92, 197, 455 |
| SetI | ASST | 8 cut(s) | 19, 57, 126, 207, 288, 429, 518, 564 |
| SfcI | CTRYAG | 2 cut(s) | 18, 222 |
| Sfr303I | CCGCGG | 1 cut(s) | 117 |
| SgrBI | CCGCGG | 1 cut(s) | 117 |
| SinI | GGWCC | 2 cut(s) | 205, 526 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 377 |
| SmlI | CTYRAG | 1 cut(s) | 12 |
| SmoI | CTYRAG | 1 cut(s) | 12 |
| Sse9I | AATT | 3 cut(s) | 386, 570, 616 |
| SspMI | CTAG | 4 cut(s) | 177, 428, 464, 533 |
| TaaI | ACNGT | 1 cut(s) | 149 |
| TaiI | ACGT | 1 cut(s) | 126 |
| TaqI | TCGA | 1 cut(s) | 390 |
| TasI | AATT | 3 cut(s) | 386, 570, 616 |
| TauI | GCSGC | 1 cut(s) | 119 |
| TfiI | GAWTC | 1 cut(s) | 185 |
| Tru1I | TTAA | 3 cut(s) | 126, 138, 144 |
| Tru9I | TTAA | 3 cut(s) | 126, 138, 144 |
| TscAI | CASTG | 4 cut(s) | 31, 414, 514, 662 |
| TseFI | GTSAC | 2 cut(s) | 50, 602 |
| TseI | GCWGC | 2 cut(s) | 224, 243 |
| Tsp45I | GTSAC | 2 cut(s) | 50, 602 |
| TspGWI | ACGGA | 1 cut(s) | 73 |
| TspRI | CASTG | 4 cut(s) | 31, 414, 514, 662 |
| VpaK11BI | GGWCC | 2 cut(s) | 205, 526 |
| XapI | RAATTY | 1 cut(s) | 386 |
| XceI | RCATGY | 1 cut(s) | 693 |
| XspI | CTAG | 4 cut(s) | 177, 428, 464, 533 |