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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 265 |
| Acc36I | ACCTGC | 2 cut(s) | 265, 412 |
| Acc65I | GGTACC | 3 cut(s) | 177, 397, 424 |
| AccB1I | GGYRCC | 3 cut(s) | 177, 397, 424 |
| AccI | GTMKAC | 1 cut(s) | 376 |
| AccII | CGCG | 1 cut(s) | 237 |
| AciI | CCGC | 9 cut(s) | 9, 15, 48, 140, 358, 390, 428, 455, 514 |
| AclI | AACGTT | 2 cut(s) | 81, 99 |
| AcoI | YGGCCR | 1 cut(s) | 279 |
| AfaI | GTAC | 3 cut(s) | 179, 399, 426 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 139, 274, 478 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AluBI | AGCT | 2 cut(s) | 77, 199 |
| AluI | AGCT | 2 cut(s) | 77, 199 |
| Alw21I | GWGCWC | 1 cut(s) | 365 |
| Alw26I | GTCTC | 1 cut(s) | 137 |
| AoxI | GGCC | 5 cut(s) | 40, 258, 264, 279, 451 |
| ApaI | GGGCCC | 1 cut(s) | 455 |
| ApeKI | GCWGC | 4 cut(s) | 31, 74, 156, 185 |
| Asp718I | GGTACC | 3 cut(s) | 177, 397, 424 |
| AspLEI | GCGC | 3 cut(s) | 237, 249, 319 |
| AspS9I | GGNCC | 4 cut(s) | 40, 265, 451, 452 |
| AsuC2I | CCSGG | 1 cut(s) | 153 |
| BaeGI | GKGCMC | 1 cut(s) | 455 |
| BalI | TGGCCA | 1 cut(s) | 281 |
| BanI | GGYRCC | 3 cut(s) | 177, 397, 424 |
| BanII | GRGCYC | 1 cut(s) | 455 |
| Bbv12I | GWGCWC | 1 cut(s) | 365 |
| BbvI | GCAGC | 4 cut(s) | 43, 86, 168, 197 |
| BccI | CCATC | 3 cut(s) | 133, 442, 472 |
| BcnI | CCSGG | 1 cut(s) | 153 |
| BcoDI | GTCTC | 1 cut(s) | 137 |
| BfaI | CTAG | 4 cut(s) | 6, 71, 89, 366 |
| BfoI | RGCGCY | 1 cut(s) | 320 |
| BfuAI | ACCTGC | 2 cut(s) | 265, 412 |
| BisI | GCNGC | 4 cut(s) | 32, 75, 157, 186 |
| BlpI | GCTNAGC | 1 cut(s) | 27 |
| BlsI | GCNGC | 4 cut(s) | 33, 76, 158, 187 |
| Bme1390I | CCNGG | 1 cut(s) | 153 |
| BmgT120I | GGNCC | 4 cut(s) | 40, 265, 451, 452 |
| BmiI | GGNNCC | 4 cut(s) | 179, 399, 426, 453 |
| BmrFI | CCNGG | 1 cut(s) | 153 |
| BmsI | GCATC | 1 cut(s) | 408 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 1 cut(s) | 153 |
| BsaI | GGTCTC | 1 cut(s) | 137 |
| BsaJI | CCNNGG | 1 cut(s) | 261 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 139, 274, 478 |
| Bse1I | ACTGG | 2 cut(s) | 43, 407 |
| Bse3DI | GCAATG | 1 cut(s) | 359 |
| BseDI | CCNNGG | 1 cut(s) | 261 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 139, 274, 478 |
| BseMI | GCAATG | 1 cut(s) | 359 |
| BseMII | CTCAG | 1 cut(s) | 41 |
| BseNI | ACTGG | 2 cut(s) | 43, 407 |
| BseSI | GKGCMC | 1 cut(s) | 455 |
| BseXI | GCAGC | 4 cut(s) | 43, 86, 168, 197 |
| BseYI | CCCAGC | 1 cut(s) | 147 |
| Bsh1236I | CGCG | 1 cut(s) | 237 |
| BshFI | GGCC | 5 cut(s) | 42, 260, 266, 281, 453 |
| BshNI | GGYRCC | 3 cut(s) | 177, 397, 424 |
| BsiHKAI | GWGCWC | 1 cut(s) | 365 |
| BsiSI | CCGG | 1 cut(s) | 152 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 139, 274, 478 |
| BsmAI | GTCTC | 1 cut(s) | 137 |
| BsnI | GGCC | 5 cut(s) | 42, 260, 266, 281, 453 |
| Bso31I | GGTCTC | 1 cut(s) | 137 |
| Bsp120I | GGGCCC | 1 cut(s) | 451 |
| Bsp1286I | GDGCHC | 2 cut(s) | 365, 455 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 27 |
| BspACI | CCGC | 9 cut(s) | 9, 15, 48, 140, 358, 390, 428, 455, 514 |
| BspANI | GGCC | 5 cut(s) | 42, 260, 266, 281, 453 |
| BspCNI | CTCAG | 1 cut(s) | 40 |
| BspFNI | CGCG | 1 cut(s) | 237 |
| BspLI | GGNNCC | 4 cut(s) | 179, 399, 426, 453 |
| BspMI | ACCTGC | 2 cut(s) | 265, 412 |
| BspT107I | GGYRCC | 3 cut(s) | 177, 397, 424 |
| BspTNI | GGTCTC | 1 cut(s) | 137 |
| BsrDI | GCAATG | 1 cut(s) | 359 |
| BsrI | ACTGG | 2 cut(s) | 43, 407 |
| BssECI | CCNNGG | 1 cut(s) | 261 |
| BstC8I | GCNNGC | 3 cut(s) | 201, 350, 455 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstFNI | CGCG | 1 cut(s) | 237 |
| BstH2I | RGCGCY | 1 cut(s) | 320 |
| BstHHI | GCGC | 3 cut(s) | 237, 249, 319 |
| BstMAI | GTCTC | 1 cut(s) | 137 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 162, 323, 338, 369, 427, 463 |
| BstNSI | RCATGY | 1 cut(s) | 390 |
| BstSCI | CCNGG | 1 cut(s) | 151 |
| BstSLI | GKGCMC | 1 cut(s) | 455 |
| BstUI | CGCG | 1 cut(s) | 237 |
| BstV1I | GCAGC | 4 cut(s) | 43, 86, 168, 197 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 174 |
| BsuRI | GGCC | 5 cut(s) | 42, 260, 266, 281, 453 |
| BveI | ACCTGC | 2 cut(s) | 265, 412 |
| Cac8I | GCNNGC | 3 cut(s) | 201, 350, 455 |
| CfoI | GCGC | 3 cut(s) | 237, 249, 319 |
| Cfr13I | GGNCC | 4 cut(s) | 40, 265, 451, 452 |
| CseI | GACGC | 1 cut(s) | 378 |
| Csp6I | GTAC | 3 cut(s) | 178, 398, 425 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 117, 152 |
| CviAII | CATG | 4 cut(s) | 107, 190, 283, 387 |
| CviQI | GTAC | 3 cut(s) | 178, 398, 425 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| EaeI | YGGCCR | 1 cut(s) | 279 |
| EciI | GGCGGA | 3 cut(s) | 37, 155, 373 |
| Eco24I | GRGCYC | 1 cut(s) | 455 |
| Eco31I | GGTCTC | 1 cut(s) | 137 |
| EcoT38I | GRGCYC | 1 cut(s) | 455 |
| FaeI | CATG | 4 cut(s) | 110, 193, 286, 390 |
| FaiI | YATR | 7 cut(s) | 108, 191, 195, 251, 284, 388, 413 |
| FatI | CATG | 4 cut(s) | 106, 189, 282, 386 |
| FauI | CCCGC | 2 cut(s) | 8, 462 |
| FblI | GTMKAC | 1 cut(s) | 376 |
| Fnu4HI | GCNGC | 4 cut(s) | 32, 75, 157, 186 |
| FriOI | GRGCYC | 1 cut(s) | 455 |
| Fsp4HI | GCNGC | 4 cut(s) | 32, 75, 157, 186 |
| FspBI | CTAG | 4 cut(s) | 6, 71, 89, 366 |
| GlaI | GCGC | 3 cut(s) | 236, 248, 318 |
| GluI | GCNGC | 4 cut(s) | 32, 75, 157, 186 |
| GsaI | CCCAGC | 1 cut(s) | 151 |
| HaeII | RGCGCY | 1 cut(s) | 320 |
| HaeIII | GGCC | 5 cut(s) | 42, 260, 266, 281, 453 |
| HapII | CCGG | 1 cut(s) | 152 |
| HgaI | GACGC | 1 cut(s) | 378 |
| HhaI | GCGC | 3 cut(s) | 237, 249, 319 |
| Hin1II | CATG | 4 cut(s) | 110, 193, 286, 390 |
| Hin6I | GCGC | 3 cut(s) | 235, 247, 317 |
| HinP1I | GCGC | 3 cut(s) | 235, 247, 317 |
| HincII | GTYRAC | 3 cut(s) | 85, 103, 377 |
| HindII | GTYRAC | 3 cut(s) | 85, 103, 377 |
| HinfI | GANTC | 1 cut(s) | 494 |
| HpaI | GTTAAC | 1 cut(s) | 103 |
| HpaII | CCGG | 1 cut(s) | 152 |
| Hpy166II | GTNNAC | 5 cut(s) | 22, 85, 97, 103, 377 |
| Hpy188I | TCNGA | 1 cut(s) | 211 |
| Hpy8I | GTNNAC | 5 cut(s) | 22, 85, 97, 103, 377 |
| HpyAV | CCTTC | 2 cut(s) | 250, 468 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 99 |
| HpyCH4V | TGCA | 3 cut(s) | 110, 203, 421 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 162, 323, 338, 369, 427, 463 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HpySE526I | ACGT | 2 cut(s) | 81, 99 |
| Hsp92II | CATG | 4 cut(s) | 110, 193, 286, 390 |
| HspAI | GCGC | 3 cut(s) | 235, 247, 317 |
| KpnI | GGTACC | 3 cut(s) | 181, 401, 428 |
| KspAI | GTTAAC | 1 cut(s) | 103 |
| LmnI | GCTCC | 3 cut(s) | 232, 360, 481 |
| Lsp1109I | GCAGC | 4 cut(s) | 43, 86, 168, 197 |
| LweI | GCATC | 1 cut(s) | 408 |
| MaeI | CTAG | 4 cut(s) | 6, 71, 89, 366 |
| MaeII | ACGT | 2 cut(s) | 81, 99 |
| MhlI | GDGCHC | 2 cut(s) | 365, 455 |
| MlsI | TGGCCA | 1 cut(s) | 281 |
| MluCI | AATT | 1 cut(s) | 305 |
| MluNI | TGGCCA | 1 cut(s) | 281 |
| MmeI | TCCRAC | 1 cut(s) | 189 |
| MnlI | CCTC | 5 cut(s) | 31, 191, 256, 479, 481 |
| Mox20I | TGGCCA | 1 cut(s) | 281 |
| MscI | TGGCCA | 1 cut(s) | 281 |
| MseI | TTAA | 3 cut(s) | 102, 299, 308 |
| MslI | CAYNNNNRTG | 2 cut(s) | 287, 416 |
| Msp20I | TGGCCA | 1 cut(s) | 281 |
| MspA1I | CMGCKG | 1 cut(s) | 430 |
| MspI | CCGG | 1 cut(s) | 152 |
| MspR9I | CCNGG | 1 cut(s) | 153 |
| MvnI | CGCG | 1 cut(s) | 237 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 162, 323, 338, 369, 427, 463 |
| NciI | CCSGG | 1 cut(s) | 153 |
| NlaIII | CATG | 4 cut(s) | 110, 193, 286, 390 |
| NlaIV | GGNNCC | 4 cut(s) | 179, 399, 426, 453 |
| NmeAIII | GCCGAG | 1 cut(s) | 286 |
| NspI | RCATGY | 1 cut(s) | 390 |
| PaqCI | CACCTGC | 1 cut(s) | 265 |
| PfeI | GAWTC | 1 cut(s) | 494 |
| PflFI | GACNNNGTC | 1 cut(s) | 372 |
| PkrI | GCNGC | 4 cut(s) | 33, 76, 158, 187 |
| Psp1406I | AACGTT | 2 cut(s) | 81, 99 |
| PspFI | CCCAGC | 1 cut(s) | 147 |
| PspN4I | GGNNCC | 4 cut(s) | 179, 399, 426, 453 |
| PspOMI | GGGCCC | 1 cut(s) | 451 |
| PspPI | GGNCC | 4 cut(s) | 40, 265, 451, 452 |
| PsyI | GACNNNGTC | 1 cut(s) | 372 |
| RsaI | GTAC | 3 cut(s) | 179, 399, 426 |
| RsaNI | GTAC | 3 cut(s) | 178, 398, 425 |
| RseI | CAYNNNNRTG | 2 cut(s) | 287, 416 |
| SalI | GTCGAC | 1 cut(s) | 375 |
| SaqAI | TTAA | 3 cut(s) | 102, 299, 308 |
| SatI | GCNGC | 4 cut(s) | 32, 75, 157, 186 |
| Sau96I | GGNCC | 4 cut(s) | 40, 265, 451, 452 |
| ScrFI | CCNGG | 1 cut(s) | 153 |
| SduI | GDGCHC | 2 cut(s) | 365, 455 |
| SfaNI | GCATC | 1 cut(s) | 408 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 287, 416 |
| Sse9I | AATT | 1 cut(s) | 305 |
| SsiI | CCGC | 9 cut(s) | 9, 15, 48, 140, 358, 390, 428, 455, 514 |
| SspMI | CTAG | 4 cut(s) | 6, 71, 89, 366 |
| StyD4I | CCNGG | 1 cut(s) | 151 |
| TaiI | ACGT | 2 cut(s) | 84, 102 |
| TaqI | TCGA | 1 cut(s) | 376 |
| TasI | AATT | 1 cut(s) | 305 |
| TfiI | GAWTC | 1 cut(s) | 494 |
| Tru1I | TTAA | 3 cut(s) | 102, 299, 308 |
| Tru9I | TTAA | 3 cut(s) | 102, 299, 308 |
| TseI | GCWGC | 4 cut(s) | 31, 74, 156, 185 |
| Tth111I | GACNNNGTC | 1 cut(s) | 372 |
| XceI | RCATGY | 1 cut(s) | 390 |
| XmiI | GTMKAC | 1 cut(s) | 376 |
| XspI | CTAG | 4 cut(s) | 6, 71, 89, 366 |