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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 451 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 505 |
| AccBSI | CCGCTC | 2 cut(s) | 399, 580 |
| AccI | GTMKAC | 1 cut(s) | 357 |
| AccII | CGCG | 2 cut(s) | 74, 90 |
| AclWI | GGATC | 3 cut(s) | 201, 422, 532 |
| AcoI | YGGCCR | 3 cut(s) | 108, 154, 305 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AcyI | GRCGYC | 1 cut(s) | 555 |
| AfaI | GTAC | 1 cut(s) | 176 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 77, 367, 414, 505, 597 |
| AflIII | ACRYGT | 1 cut(s) | 277 |
| AgeI | ACCGGT | 1 cut(s) | 367 |
| AjnI | CCWGG | 3 cut(s) | 346, 407, 595 |
| Alw26I | GTCTC | 1 cut(s) | 542 |
| AlwI | GGATC | 3 cut(s) | 201, 422, 532 |
| AoxI | GGCC | 7 cut(s) | 108, 154, 240, 305, 449, 471, 599 |
| ApaI | GGGCCC | 1 cut(s) | 603 |
| ApeKI | GCWGC | 4 cut(s) | 138, 141, 263, 611 |
| AsiGI | ACCGGT | 1 cut(s) | 367 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 381 |
| AspLEI | GCGC | 5 cut(s) | 92, 231, 268, 447, 565 |
| AspS9I | GGNCC | 2 cut(s) | 599, 600 |
| AsuC2I | CCSGG | 5 cut(s) | 107, 117, 319, 457, 542 |
| AsuHPI | GGTGA | 2 cut(s) | 134, 437 |
| BaeGI | GKGCMC | 1 cut(s) | 603 |
| BanII | GRGCYC | 1 cut(s) | 603 |
| BbvI | GCAGC | 4 cut(s) | 150, 153, 250, 598 |
| BccI | CCATC | 4 cut(s) | 59, 157, 253, 460 |
| BciT130I | CCWGG | 3 cut(s) | 348, 409, 597 |
| BcnI | CCSGG | 5 cut(s) | 107, 117, 319, 457, 542 |
| BcoDI | GTCTC | 1 cut(s) | 542 |
| BfmI | CTRYAG | 2 cut(s) | 10, 620 |
| BfoI | RGCGCY | 1 cut(s) | 566 |
| BfuAI | ACCTGC | 1 cut(s) | 451 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 457 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 305, 612 |
| BlsI | GCNGC | 6 cut(s) | 140, 143, 146, 265, 306, 613 |
| Bme1390I | CCNGG | 8 cut(s) | 107, 117, 319, 348, 409, 457, 542, 597 |
| BmgT120I | GGNCC | 2 cut(s) | 599, 600 |
| BmiI | GGNNCC | 1 cut(s) | 601 |
| BmrFI | CCNGG | 8 cut(s) | 107, 117, 319, 348, 409, 457, 542, 597 |
| BmsI | GCATC | 1 cut(s) | 504 |
| BoxI | GACNNNNGTC | 1 cut(s) | 554 |
| BpmI | CTGGAG | 1 cut(s) | 545 |
| BpuMI | CCSGG | 5 cut(s) | 107, 117, 319, 457, 542 |
| BsaHI | GRCGYC | 1 cut(s) | 555 |
| BsaJI | CCNNGG | 5 cut(s) | 105, 115, 408, 440, 596 |
| BsaWI | WCCGGW | 1 cut(s) | 367 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 518, 548 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 77, 367, 414, 505, 597 |
| Bse118I | RCCGGY | 1 cut(s) | 367 |
| Bse1I | ACTGG | 2 cut(s) | 192, 528 |
| BseBI | CCWGG | 3 cut(s) | 348, 409, 597 |
| BseDI | CCNNGG | 5 cut(s) | 105, 115, 408, 440, 596 |
| BseGI | GGATG | 5 cut(s) | 51, 149, 452, 491, 519 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 77, 367, 414, 505, 597 |
| BseNI | ACTGG | 2 cut(s) | 192, 528 |
| BseSI | GKGCMC | 1 cut(s) | 603 |
| BseXI | GCAGC | 4 cut(s) | 150, 153, 250, 598 |
| BseYI | CCCAGC | 2 cut(s) | 361, 559 |
| Bsh1236I | CGCG | 2 cut(s) | 74, 90 |
| Bsh1285I | CGRYCG | 1 cut(s) | 78 |
| BshFI | GGCC | 7 cut(s) | 110, 156, 242, 307, 451, 473, 601 |
| BshTI | ACCGGT | 1 cut(s) | 367 |
| BsiEI | CGRYCG | 1 cut(s) | 78 |
| BsiSI | CCGG | 7 cut(s) | 107, 117, 153, 319, 368, 457, 541 |
| BslFI | GGGAC | 1 cut(s) | 542 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 77, 367, 414, 505, 597 |
| BsmAI | GTCTC | 1 cut(s) | 542 |
| BsmBI | CGTCTC | 1 cut(s) | 542 |
| BsmFI | GGGAC | 1 cut(s) | 542 |
| BsnI | GGCC | 7 cut(s) | 110, 156, 242, 307, 451, 473, 601 |
| Bsp120I | GGGCCC | 1 cut(s) | 599 |
| Bsp1286I | GDGCHC | 1 cut(s) | 603 |
| Bsp143I | GATC | 5 cut(s) | 75, 160, 193, 427, 537 |
| BspANI | GGCC | 7 cut(s) | 110, 156, 242, 307, 451, 473, 601 |
| BspFNI | CGCG | 2 cut(s) | 74, 90 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspLI | GGNNCC | 1 cut(s) | 601 |
| BspMI | ACCTGC | 1 cut(s) | 451 |
| BspPI | GGATC | 3 cut(s) | 201, 422, 532 |
| BspQI | GCTCTTC | 2 cut(s) | 24, 587 |
| BsrBI | CCGCTC | 2 cut(s) | 399, 580 |
| BsrFI | RCCGGY | 1 cut(s) | 367 |
| BsrI | ACTGG | 2 cut(s) | 192, 528 |
| BssAI | RCCGGY | 1 cut(s) | 367 |
| BssECI | CCNNGG | 5 cut(s) | 105, 115, 408, 440, 596 |
| BssMI | GATC | 5 cut(s) | 75, 160, 193, 427, 537 |
| BssNI | GRCGYC | 1 cut(s) | 555 |
| Bst2UI | CCWGG | 3 cut(s) | 348, 409, 597 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 335 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 587 |
| BstACI | GRCGYC | 1 cut(s) | 555 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 48, 449 |
| BstF5I | GGATG | 5 cut(s) | 51, 149, 452, 491, 519 |
| BstFNI | CGCG | 2 cut(s) | 74, 90 |
| BstH2I | RGCGCY | 1 cut(s) | 566 |
| BstHHI | GCGC | 5 cut(s) | 92, 231, 268, 447, 565 |
| BstKTI | GATC | 5 cut(s) | 78, 163, 196, 430, 540 |
| BstMAI | GTCTC | 1 cut(s) | 542 |
| BstMBI | GATC | 5 cut(s) | 75, 160, 193, 427, 537 |
| BstMCI | CGRYCG | 1 cut(s) | 78 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 87, 144, 153, 371, 457, 479 |
| BstNI | CCWGG | 3 cut(s) | 348, 409, 597 |
| BstNSI | RCATGY | 1 cut(s) | 281 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 554 |
| BstSCI | CCNGG | 8 cut(s) | 105, 115, 317, 346, 407, 455, 540, 595 |
| BstSFI | CTRYAG | 2 cut(s) | 10, 620 |
| BstSLI | GKGCMC | 1 cut(s) | 603 |
| BstUI | CGCG | 2 cut(s) | 74, 90 |
| BstV1I | GCAGC | 4 cut(s) | 150, 153, 250, 598 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 7 cut(s) | 110, 156, 242, 307, 451, 473, 601 |
| BtsCI | GGATG | 5 cut(s) | 51, 149, 452, 491, 519 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| BveI | ACCTGC | 1 cut(s) | 451 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 48, 449 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 5 cut(s) | 92, 231, 268, 447, 565 |
| Cfr10I | RCCGGY | 1 cut(s) | 367 |
| Cfr13I | GGNCC | 2 cut(s) | 599, 600 |
| CseI | GACGC | 1 cut(s) | 544 |
| Csp6I | GTAC | 1 cut(s) | 175 |
| CspAI | ACCGGT | 1 cut(s) | 367 |
| CviAII | CATG | 3 cut(s) | 271, 278, 338 |
| CviQI | GTAC | 1 cut(s) | 175 |
| DpnI | GATC | 5 cut(s) | 77, 162, 195, 429, 539 |
| DpnII | GATC | 5 cut(s) | 75, 160, 193, 427, 537 |
| EaeI | YGGCCR | 3 cut(s) | 108, 154, 305 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 587 |
| EarI | CTCTTC | 2 cut(s) | 24, 587 |
| EciI | GGCGGA | 7 cut(s) | 206, 239, 389, 420, 469, 484, 560 |
| Eco24I | GRGCYC | 1 cut(s) | 603 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoO109I | RGGNCCY | 1 cut(s) | 600 |
| EcoRII | CCWGG | 3 cut(s) | 346, 407, 595 |
| EcoT38I | GRGCYC | 1 cut(s) | 603 |
| Esp3I | CGTCTC | 1 cut(s) | 542 |
| FaeI | CATG | 3 cut(s) | 274, 281, 341 |
| FaiI | YATR | 5 cut(s) | 173, 201, 272, 279, 339 |
| FaqI | GGGAC | 1 cut(s) | 542 |
| FatI | CATG | 3 cut(s) | 270, 277, 337 |
| FauI | CCCGC | 3 cut(s) | 8, 55, 79 |
| FblI | GTMKAC | 1 cut(s) | 357 |
| Fnu4HI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 305, 612 |
| FokI | GGATG | 5 cut(s) | 38, 136, 439, 478, 526 |
| FriOI | GRGCYC | 1 cut(s) | 603 |
| Fsp4HI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 305, 612 |
| GlaI | GCGC | 5 cut(s) | 91, 230, 267, 446, 564 |
| GluI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 305, 612 |
| GsaI | CCCAGC | 2 cut(s) | 365, 563 |
| GsuI | CTGGAG | 1 cut(s) | 545 |
| HaeII | RGCGCY | 1 cut(s) | 566 |
| HaeIII | GGCC | 7 cut(s) | 110, 156, 242, 307, 451, 473, 601 |
| HapII | CCGG | 7 cut(s) | 107, 117, 153, 319, 368, 457, 541 |
| HgaI | GACGC | 1 cut(s) | 544 |
| HhaI | GCGC | 5 cut(s) | 92, 231, 268, 447, 565 |
| Hin1I | GRCGYC | 1 cut(s) | 555 |
| Hin1II | CATG | 3 cut(s) | 274, 281, 341 |
| Hin6I | GCGC | 5 cut(s) | 90, 229, 266, 445, 563 |
| HinP1I | GCGC | 5 cut(s) | 90, 229, 266, 445, 563 |
| HincII | GTYRAC | 2 cut(s) | 85, 358 |
| HindII | GTYRAC | 2 cut(s) | 85, 358 |
| HinfI | GANTC | 4 cut(s) | 297, 377, 476, 574 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 7 cut(s) | 107, 117, 153, 319, 368, 457, 541 |
| HphI | GGTGA | 2 cut(s) | 134, 437 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 358 |
| Hpy188I | TCNGA | 4 cut(s) | 67, 135, 403, 573 |
| Hpy188III | TCNNGA | 1 cut(s) | 271 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 358 |
| Hpy99I | CGWCG | 1 cut(s) | 488 |
| HpyAV | CCTTC | 1 cut(s) | 72 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 335 |
| HpyCH4IV | ACGT | 1 cut(s) | 217 |
| HpyCH4V | TGCA | 2 cut(s) | 517, 614 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 87, 144, 153, 371, 457, 479 |
| HpySE526I | ACGT | 1 cut(s) | 217 |
| Hsp92I | GRCGYC | 1 cut(s) | 555 |
| Hsp92II | CATG | 3 cut(s) | 274, 281, 341 |
| HspAI | GCGC | 5 cut(s) | 90, 229, 266, 445, 563 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 5 cut(s) | 75, 160, 193, 427, 537 |
| LguI | GCTCTTC | 2 cut(s) | 24, 587 |
| LmnI | GCTCC | 2 cut(s) | 226, 526 |
| Lsp1109I | GCAGC | 4 cut(s) | 150, 153, 250, 598 |
| LweI | GCATC | 1 cut(s) | 504 |
| MaeII | ACGT | 1 cut(s) | 217 |
| MaeIII | GTNAC | 1 cut(s) | 166 |
| MalI | GATC | 5 cut(s) | 77, 162, 195, 429, 539 |
| MbiI | CCGCTC | 2 cut(s) | 399, 580 |
| MboI | GATC | 5 cut(s) | 75, 160, 193, 427, 537 |
| MboII | GAAGA | 3 cut(s) | 41, 188, 574 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 1 cut(s) | 603 |
| MluCI | AATT | 1 cut(s) | 615 |
| MlyI | GAGTC | 1 cut(s) | 291 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MnlI | CCTC | 6 cut(s) | 118, 214, 277, 408, 435, 525 |
| MroXI | GAANNNNTTC | 1 cut(s) | 381 |
| MseI | TTAA | 1 cut(s) | 84 |
| MspA1I | CMGCKG | 1 cut(s) | 611 |
| MspI | CCGG | 7 cut(s) | 107, 117, 153, 319, 368, 457, 541 |
| MspR9I | CCNGG | 8 cut(s) | 107, 117, 319, 348, 409, 457, 542, 597 |
| MvaI | CCWGG | 3 cut(s) | 348, 409, 597 |
| MvnI | CGCG | 2 cut(s) | 74, 90 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 87, 144, 153, 371, 457, 479 |
| NciI | CCSGG | 5 cut(s) | 107, 117, 319, 457, 542 |
| NdeII | GATC | 5 cut(s) | 75, 160, 193, 427, 537 |
| NlaIII | CATG | 3 cut(s) | 274, 281, 341 |
| NlaIV | GGNNCC | 1 cut(s) | 601 |
| NmeAIII | GCCGAG | 1 cut(s) | 465 |
| NmuCI | GTSAC | 1 cut(s) | 166 |
| NspI | RCATGY | 1 cut(s) | 281 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PciI | ACATGT | 1 cut(s) | 277 |
| PciSI | GCTCTTC | 2 cut(s) | 24, 587 |
| PdmI | GAANNNNTTC | 1 cut(s) | 381 |
| PfeI | GAWTC | 3 cut(s) | 377, 476, 574 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 505 |
| PinAI | ACCGGT | 1 cut(s) | 367 |
| PkrI | GCNGC | 6 cut(s) | 140, 143, 146, 265, 306, 613 |
| Ple19I | CGATCG | 1 cut(s) | 78 |
| PleI | GAGTC | 1 cut(s) | 291 |
| PpsI | GAGTC | 1 cut(s) | 291 |
| PscI | ACATGT | 1 cut(s) | 277 |
| PshAI | GACNNNNGTC | 1 cut(s) | 554 |
| Psp6I | CCWGG | 3 cut(s) | 346, 407, 595 |
| PspFI | CCCAGC | 2 cut(s) | 361, 559 |
| PspGI | CCWGG | 3 cut(s) | 346, 407, 595 |
| PspN4I | GGNNCC | 1 cut(s) | 601 |
| PspOMI | GGGCCC | 1 cut(s) | 599 |
| PspPI | GGNCC | 2 cut(s) | 599, 600 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PvuI | CGATCG | 1 cut(s) | 78 |
| RsaI | GTAC | 1 cut(s) | 176 |
| RsaNI | GTAC | 1 cut(s) | 175 |
| SalI | GTCGAC | 1 cut(s) | 356 |
| SapI | GCTCTTC | 2 cut(s) | 24, 587 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 6 cut(s) | 139, 142, 145, 264, 305, 612 |
| Sau3AI | GATC | 5 cut(s) | 75, 160, 193, 427, 537 |
| Sau96I | GGNCC | 2 cut(s) | 599, 600 |
| SchI | GAGTC | 1 cut(s) | 291 |
| ScrFI | CCNGG | 8 cut(s) | 107, 117, 319, 348, 409, 457, 542, 597 |
| SduI | GDGCHC | 1 cut(s) | 603 |
| SetI | ASST | 6 cut(s) | 64, 220, 427, 465, 536, 598 |
| SfaNI | GCATC | 1 cut(s) | 504 |
| SfcI | CTRYAG | 2 cut(s) | 10, 620 |
| SgrAI | CRCCGGYG | 1 cut(s) | 367 |
| Sse9I | AATT | 1 cut(s) | 615 |
| StyD4I | CCNGG | 8 cut(s) | 105, 115, 317, 346, 407, 455, 540, 595 |
| TaaI | ACNGT | 2 cut(s) | 11, 335 |
| TaiI | ACGT | 1 cut(s) | 220 |
| TaqI | TCGA | 1 cut(s) | 357 |
| TasI | AATT | 1 cut(s) | 615 |
| TauI | GCSGC | 2 cut(s) | 147, 307 |
| TfiI | GAWTC | 3 cut(s) | 377, 476, 574 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 1 cut(s) | 166 |
| TseI | GCWGC | 4 cut(s) | 138, 141, 263, 611 |
| Tsp45I | GTSAC | 1 cut(s) | 166 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspRI | CASTG | 1 cut(s) | 192 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 505 |
| XceI | RCATGY | 1 cut(s) | 281 |
| XmiI | GTMKAC | 1 cut(s) | 357 |
| XmnI | GAANNNNTTC | 1 cut(s) | 381 |