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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 265 |
| Acc36I | ACCTGC | 2 cut(s) | 265, 412 |
| Acc65I | GGTACC | 1 cut(s) | 424 |
| AccB1I | GGYRCC | 1 cut(s) | 424 |
| AciI | CCGC | 7 cut(s) | 9, 15, 38, 140, 428, 455, 477 |
| AclI | AACGTT | 2 cut(s) | 81, 99 |
| AclWI | GGATC | 1 cut(s) | 186 |
| AcoI | YGGCCR | 1 cut(s) | 279 |
| AfaI | GTAC | 3 cut(s) | 399, 405, 426 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 139, 220, 274, 386, 442, 464 |
| AgeI | ACCGGT | 1 cut(s) | 169 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AjnI | CCWGG | 1 cut(s) | 174 |
| AluBI | AGCT | 4 cut(s) | 77, 188, 199, 510 |
| AluI | AGCT | 4 cut(s) | 77, 188, 199, 510 |
| Alw21I | GWGCWC | 1 cut(s) | 365 |
| Alw26I | GTCTC | 1 cut(s) | 137 |
| AlwI | GGATC | 1 cut(s) | 186 |
| AoxI | GGCC | 4 cut(s) | 264, 279, 377, 451 |
| ApaI | GGGCCC | 1 cut(s) | 455 |
| ApeKI | GCWGC | 5 cut(s) | 31, 74, 156, 185, 507 |
| AsiGI | ACCGGT | 1 cut(s) | 169 |
| Asp718I | GGTACC | 1 cut(s) | 424 |
| AspLEI | GCGC | 2 cut(s) | 237, 249 |
| AspS9I | GGNCC | 6 cut(s) | 40, 172, 265, 378, 451, 452 |
| AsuC2I | CCSGG | 2 cut(s) | 153, 504 |
| AsuHPI | GGTGA | 2 cut(s) | 155, 457 |
| AvaII | GGWCC | 2 cut(s) | 40, 172 |
| BaeGI | GKGCMC | 1 cut(s) | 455 |
| BalI | TGGCCA | 1 cut(s) | 281 |
| BanI | GGYRCC | 1 cut(s) | 424 |
| BanII | GRGCYC | 1 cut(s) | 455 |
| Bbv12I | GWGCWC | 1 cut(s) | 365 |
| BbvI | GCAGC | 4 cut(s) | 43, 86, 168, 197 |
| BccI | CCATC | 4 cut(s) | 133, 225, 442, 458 |
| BciT130I | CCWGG | 1 cut(s) | 176 |
| BcnI | CCSGG | 2 cut(s) | 153, 504 |
| BcoDI | GTCTC | 1 cut(s) | 137 |
| BfaI | CTAG | 2 cut(s) | 6, 71 |
| BfuAI | ACCTGC | 2 cut(s) | 265, 412 |
| BisI | GCNGC | 5 cut(s) | 32, 75, 157, 186, 508 |
| BlpI | GCTNAGC | 1 cut(s) | 27 |
| BlsI | GCNGC | 5 cut(s) | 33, 76, 158, 187, 509 |
| Bme1390I | CCNGG | 3 cut(s) | 153, 176, 504 |
| Bme18I | GGWCC | 2 cut(s) | 40, 172 |
| BmgT120I | GGNCC | 6 cut(s) | 40, 172, 265, 378, 451, 452 |
| BmiI | GGNNCC | 2 cut(s) | 426, 453 |
| BmrFI | CCNGG | 3 cut(s) | 153, 176, 504 |
| BmsI | GCATC | 1 cut(s) | 408 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 2 cut(s) | 153, 504 |
| BsaI | GGTCTC | 1 cut(s) | 137 |
| BsaJI | CCNNGG | 1 cut(s) | 261 |
| BsaWI | WCCGGW | 1 cut(s) | 169 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 139, 220, 274, 386, 442, 464 |
| Bse118I | RCCGGY | 1 cut(s) | 169 |
| Bse1I | ACTGG | 3 cut(s) | 43, 324, 407 |
| BseBI | CCWGG | 1 cut(s) | 176 |
| BseDI | CCNNGG | 1 cut(s) | 261 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 139, 220, 274, 386, 442, 464 |
| BseMII | CTCAG | 1 cut(s) | 41 |
| BseNI | ACTGG | 3 cut(s) | 43, 324, 407 |
| BseSI | GKGCMC | 1 cut(s) | 455 |
| BseXI | GCAGC | 4 cut(s) | 43, 86, 168, 197 |
| BseYI | CCCAGC | 1 cut(s) | 147 |
| BshFI | GGCC | 4 cut(s) | 266, 281, 379, 453 |
| BshNI | GGYRCC | 1 cut(s) | 424 |
| BshTI | ACCGGT | 1 cut(s) | 169 |
| BsiHKAI | GWGCWC | 1 cut(s) | 365 |
| BsiSI | CCGG | 3 cut(s) | 152, 170, 504 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 139, 220, 274, 386, 442, 464 |
| BsmAI | GTCTC | 1 cut(s) | 137 |
| BsnI | GGCC | 4 cut(s) | 266, 281, 379, 453 |
| Bso31I | GGTCTC | 1 cut(s) | 137 |
| Bsp120I | GGGCCC | 1 cut(s) | 451 |
| Bsp1286I | GDGCHC | 2 cut(s) | 365, 455 |
| Bsp143I | GATC | 1 cut(s) | 178 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 27 |
| BspACI | CCGC | 7 cut(s) | 9, 15, 38, 140, 428, 455, 477 |
| BspANI | GGCC | 4 cut(s) | 266, 281, 379, 453 |
| BspCNI | CTCAG | 1 cut(s) | 40 |
| BspLI | GGNNCC | 2 cut(s) | 426, 453 |
| BspMI | ACCTGC | 2 cut(s) | 265, 412 |
| BspPI | GGATC | 1 cut(s) | 186 |
| BspT107I | GGYRCC | 1 cut(s) | 424 |
| BspTNI | GGTCTC | 1 cut(s) | 137 |
| BsrFI | RCCGGY | 1 cut(s) | 169 |
| BsrI | ACTGG | 3 cut(s) | 43, 324, 407 |
| BssAI | RCCGGY | 1 cut(s) | 169 |
| BssECI | CCNNGG | 1 cut(s) | 261 |
| BssMI | GATC | 1 cut(s) | 178 |
| Bst2UI | CCWGG | 1 cut(s) | 176 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 201, 350, 455 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 218 |
| BstHHI | GCGC | 2 cut(s) | 237, 249 |
| BstKTI | GATC | 1 cut(s) | 181 |
| BstMAI | GTCTC | 1 cut(s) | 137 |
| BstMBI | GATC | 1 cut(s) | 178 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 323, 369, 427 |
| BstNI | CCWGG | 1 cut(s) | 176 |
| BstSCI | CCNGG | 3 cut(s) | 151, 174, 502 |
| BstSLI | GKGCMC | 1 cut(s) | 455 |
| BstV1I | GCAGC | 4 cut(s) | 43, 86, 168, 197 |
| BstX2I | RGATCY | 1 cut(s) | 178 |
| BstYI | RGATCY | 1 cut(s) | 178 |
| BsuRI | GGCC | 4 cut(s) | 266, 281, 379, 453 |
| BtsIMutI | CAGTG | 1 cut(s) | 317 |
| BveI | ACCTGC | 2 cut(s) | 265, 412 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 201, 350, 455 |
| CfoI | GCGC | 2 cut(s) | 237, 249 |
| Cfr10I | RCCGGY | 1 cut(s) | 169 |
| Cfr13I | GGNCC | 6 cut(s) | 40, 172, 265, 378, 451, 452 |
| CseI | GACGC | 1 cut(s) | 378 |
| Csp6I | GTAC | 3 cut(s) | 398, 404, 425 |
| CspAI | ACCGGT | 1 cut(s) | 169 |
| CviAII | CATG | 1 cut(s) | 107 |
| CviQI | GTAC | 3 cut(s) | 398, 404, 425 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 1 cut(s) | 180 |
| DpnII | GATC | 1 cut(s) | 178 |
| EaeI | YGGCCR | 1 cut(s) | 279 |
| EciI | GGCGGA | 1 cut(s) | 492 |
| Eco24I | GRGCYC | 1 cut(s) | 455 |
| Eco31I | GGTCTC | 1 cut(s) | 137 |
| Eco47I | GGWCC | 2 cut(s) | 40, 172 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 218 |
| EcoRII | CCWGG | 1 cut(s) | 174 |
| EcoT38I | GRGCYC | 1 cut(s) | 455 |
| FaeI | CATG | 1 cut(s) | 110 |
| FaiI | YATR | 6 cut(s) | 108, 191, 195, 211, 251, 413 |
| FatI | CATG | 1 cut(s) | 106 |
| FauI | CCCGC | 2 cut(s) | 8, 462 |
| Fnu4HI | GCNGC | 5 cut(s) | 32, 75, 157, 186, 508 |
| FriOI | GRGCYC | 1 cut(s) | 455 |
| Fsp4HI | GCNGC | 5 cut(s) | 32, 75, 157, 186, 508 |
| FspBI | CTAG | 2 cut(s) | 6, 71 |
| GlaI | GCGC | 2 cut(s) | 236, 248 |
| GluI | GCNGC | 5 cut(s) | 32, 75, 157, 186, 508 |
| GsaI | CCCAGC | 1 cut(s) | 151 |
| HaeIII | GGCC | 4 cut(s) | 266, 281, 379, 453 |
| HapII | CCGG | 3 cut(s) | 152, 170, 504 |
| HgaI | GACGC | 1 cut(s) | 378 |
| HhaI | GCGC | 2 cut(s) | 237, 249 |
| Hin1II | CATG | 1 cut(s) | 110 |
| Hin6I | GCGC | 2 cut(s) | 235, 247 |
| HinP1I | GCGC | 2 cut(s) | 235, 247 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HinfI | GANTC | 1 cut(s) | 480 |
| HpaII | CCGG | 3 cut(s) | 152, 170, 504 |
| HphI | GGTGA | 2 cut(s) | 155, 457 |
| Hpy166II | GTNNAC | 2 cut(s) | 48, 85 |
| Hpy8I | GTNNAC | 2 cut(s) | 48, 85 |
| Hpy99I | CGWCG | 1 cut(s) | 305 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 99 |
| HpyCH4V | TGCA | 2 cut(s) | 110, 421 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 323, 369, 427 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HpySE526I | ACGT | 2 cut(s) | 81, 99 |
| Hsp92II | CATG | 1 cut(s) | 110 |
| HspAI | GCGC | 2 cut(s) | 235, 247 |
| KpnI | GGTACC | 1 cut(s) | 428 |
| Kzo9I | GATC | 1 cut(s) | 178 |
| LmnI | GCTCC | 3 cut(s) | 232, 360, 467 |
| Lsp1109I | GCAGC | 4 cut(s) | 43, 86, 168, 197 |
| LweI | GCATC | 1 cut(s) | 408 |
| MaeI | CTAG | 2 cut(s) | 6, 71 |
| MaeII | ACGT | 2 cut(s) | 81, 99 |
| MaeIII | GTNAC | 1 cut(s) | 161 |
| MalI | GATC | 1 cut(s) | 180 |
| MboI | GATC | 1 cut(s) | 178 |
| MflI | RGATCY | 1 cut(s) | 178 |
| MhlI | GDGCHC | 2 cut(s) | 365, 455 |
| MlsI | TGGCCA | 1 cut(s) | 281 |
| MluCI | AATT | 1 cut(s) | 305 |
| MluNI | TGGCCA | 1 cut(s) | 281 |
| MnlI | CCTC | 4 cut(s) | 224, 256, 436, 467 |
| Mox20I | TGGCCA | 1 cut(s) | 281 |
| MscI | TGGCCA | 1 cut(s) | 281 |
| MseI | TTAA | 2 cut(s) | 102, 308 |
| MslI | CAYNNNNRTG | 2 cut(s) | 287, 416 |
| Msp20I | TGGCCA | 1 cut(s) | 281 |
| MspA1I | CMGCKG | 2 cut(s) | 140, 430 |
| MspI | CCGG | 3 cut(s) | 152, 170, 504 |
| MspR9I | CCNGG | 3 cut(s) | 153, 176, 504 |
| MvaI | CCWGG | 1 cut(s) | 176 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 323, 369, 427 |
| NciI | CCSGG | 2 cut(s) | 153, 504 |
| NdeII | GATC | 1 cut(s) | 178 |
| NlaIII | CATG | 1 cut(s) | 110 |
| NlaIV | GGNNCC | 2 cut(s) | 426, 453 |
| NmeAIII | GCCGAG | 1 cut(s) | 286 |
| NmuCI | GTSAC | 1 cut(s) | 161 |
| PaqCI | CACCTGC | 1 cut(s) | 265 |
| PfeI | GAWTC | 1 cut(s) | 480 |
| PflFI | GACNNNGTC | 1 cut(s) | 372 |
| PfoI | TCCNGGA | 1 cut(s) | 174 |
| PinAI | ACCGGT | 1 cut(s) | 169 |
| PkrI | GCNGC | 5 cut(s) | 33, 76, 158, 187, 509 |
| Psp1406I | AACGTT | 2 cut(s) | 81, 99 |
| Psp6I | CCWGG | 1 cut(s) | 174 |
| PspFI | CCCAGC | 1 cut(s) | 147 |
| PspGI | CCWGG | 1 cut(s) | 174 |
| PspN4I | GGNNCC | 2 cut(s) | 426, 453 |
| PspOMI | GGGCCC | 1 cut(s) | 451 |
| PspPI | GGNCC | 6 cut(s) | 40, 172, 265, 378, 451, 452 |
| PsuI | RGATCY | 1 cut(s) | 178 |
| PsyI | GACNNNGTC | 1 cut(s) | 372 |
| RsaI | GTAC | 3 cut(s) | 399, 405, 426 |
| RsaNI | GTAC | 3 cut(s) | 398, 404, 425 |
| RseI | CAYNNNNRTG | 2 cut(s) | 287, 416 |
| SaqAI | TTAA | 2 cut(s) | 102, 308 |
| SatI | GCNGC | 5 cut(s) | 32, 75, 157, 186, 508 |
| Sau3AI | GATC | 1 cut(s) | 178 |
| Sau96I | GGNCC | 6 cut(s) | 40, 172, 265, 378, 451, 452 |
| ScrFI | CCNGG | 3 cut(s) | 153, 176, 504 |
| SduI | GDGCHC | 2 cut(s) | 365, 455 |
| SfaNI | GCATC | 1 cut(s) | 408 |
| SinI | GGWCC | 2 cut(s) | 40, 172 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 287, 416 |
| Sse9I | AATT | 1 cut(s) | 305 |
| SsiI | CCGC | 7 cut(s) | 9, 15, 38, 140, 428, 455, 477 |
| SspMI | CTAG | 2 cut(s) | 6, 71 |
| StyD4I | CCNGG | 3 cut(s) | 151, 174, 502 |
| TaiI | ACGT | 2 cut(s) | 84, 102 |
| TaqI | TCGA | 1 cut(s) | 300 |
| TasI | AATT | 1 cut(s) | 305 |
| TfiI | GAWTC | 1 cut(s) | 480 |
| Tru1I | TTAA | 2 cut(s) | 102, 308 |
| Tru9I | TTAA | 2 cut(s) | 102, 308 |
| TscAI | CASTG | 1 cut(s) | 324 |
| TseFI | GTSAC | 1 cut(s) | 161 |
| TseI | GCWGC | 5 cut(s) | 31, 74, 156, 185, 507 |
| Tsp45I | GTSAC | 1 cut(s) | 161 |
| TspGWI | ACGGA | 1 cut(s) | 373 |
| TspRI | CASTG | 1 cut(s) | 324 |
| Tth111I | GACNNNGTC | 1 cut(s) | 372 |
| VpaK11BI | GGWCC | 2 cut(s) | 40, 172 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 218 |
| XspI | CTAG | 2 cut(s) | 6, 71 |