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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 73, 225 |
| AciI | CCGC | 6 cut(s) | 38, 71, 73, 91, 110, 128 |
| AclI | AACGTT | 1 cut(s) | 80 |
| AclWI | GGATC | 2 cut(s) | 48, 174 |
| AcoI | YGGCCR | 1 cut(s) | 267 |
| AcsI | RAATTY | 1 cut(s) | 284 |
| AcuI | CTGAAG | 1 cut(s) | 317 |
| AjnI | CCWGG | 2 cut(s) | 157, 162 |
| AluBI | AGCT | 2 cut(s) | 187, 235 |
| AluI | AGCT | 2 cut(s) | 187, 235 |
| Alw21I | GWGCWC | 2 cut(s) | 231, 237 |
| AlwI | GGATC | 2 cut(s) | 48, 174 |
| AoxI | GGCC | 5 cut(s) | 132, 160, 246, 252, 267 |
| ApeKI | GCWGC | 1 cut(s) | 125 |
| ApoI | RAATTY | 1 cut(s) | 284 |
| AspLEI | GCGC | 2 cut(s) | 225, 331 |
| AspS9I | GGNCC | 2 cut(s) | 133, 253 |
| BalI | TGGCCA | 1 cut(s) | 269 |
| BanII | GRGCYC | 1 cut(s) | 237 |
| BauI | CACGAG | 1 cut(s) | 230 |
| Bbv12I | GWGCWC | 2 cut(s) | 231, 237 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 1 cut(s) | 137 |
| BciT130I | CCWGG | 2 cut(s) | 159, 164 |
| BfmI | CTRYAG | 1 cut(s) | 123 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 159 |
| BglII | AGATCT | 1 cut(s) | 199 |
| BisI | GCNGC | 2 cut(s) | 74, 126 |
| BlsI | GCNGC | 2 cut(s) | 75, 127 |
| Bme1390I | CCNGG | 2 cut(s) | 159, 164 |
| BmgT120I | GGNCC | 2 cut(s) | 133, 253 |
| BmrFI | CCNGG | 2 cut(s) | 159, 164 |
| BoxI | GACNNNNGTC | 1 cut(s) | 147 |
| BpmI | CTGGAG | 1 cut(s) | 329 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BsaJI | CCNNGG | 2 cut(s) | 71, 249 |
| Bse1I | ACTGG | 1 cut(s) | 312 |
| BseBI | CCWGG | 2 cut(s) | 159, 164 |
| BseDI | CCNNGG | 2 cut(s) | 71, 249 |
| BseMII | CTCAG | 2 cut(s) | 18, 250 |
| BseNI | ACTGG | 1 cut(s) | 312 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseXI | GCAGC | 1 cut(s) | 137 |
| BseYI | CCCAGC | 2 cut(s) | 87, 325 |
| Bsh1236I | CGCG | 2 cut(s) | 73, 225 |
| BshFI | GGCC | 5 cut(s) | 134, 162, 248, 254, 269 |
| BsiHKAI | GWGCWC | 2 cut(s) | 231, 237 |
| BslFI | GGGAC | 1 cut(s) | 92 |
| BsmFI | GGGAC | 1 cut(s) | 92 |
| BsnI | GGCC | 5 cut(s) | 134, 162, 248, 254, 269 |
| Bsp1286I | GDGCHC | 2 cut(s) | 231, 237 |
| Bsp143I | GATC | 3 cut(s) | 40, 166, 199 |
| BspACI | CCGC | 6 cut(s) | 38, 71, 73, 91, 110, 128 |
| BspANI | GGCC | 5 cut(s) | 134, 162, 248, 254, 269 |
| BspCNI | CTCAG | 2 cut(s) | 19, 249 |
| BspFNI | CGCG | 2 cut(s) | 73, 225 |
| BspMAI | CTGCAG | 1 cut(s) | 127 |
| BspPI | GGATC | 2 cut(s) | 48, 174 |
| BsrI | ACTGG | 1 cut(s) | 312 |
| BssECI | CCNNGG | 2 cut(s) | 71, 249 |
| BssMI | GATC | 3 cut(s) | 40, 166, 199 |
| BssSI | CACGAG | 1 cut(s) | 230 |
| Bst2BI | CACGAG | 1 cut(s) | 230 |
| Bst2UI | CCWGG | 2 cut(s) | 159, 164 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 106 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 189, 227 |
| BstDEI | CTNAG | 2 cut(s) | 27, 236 |
| BstDSI | CCRYGG | 1 cut(s) | 71 |
| BstFNI | CGCG | 2 cut(s) | 73, 225 |
| BstHHI | GCGC | 2 cut(s) | 225, 331 |
| BstKTI | GATC | 3 cut(s) | 43, 169, 202 |
| BstMBI | GATC | 3 cut(s) | 40, 166, 199 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 131, 159, 311, 326, 337 |
| BstNI | CCWGG | 2 cut(s) | 159, 164 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 147 |
| BstSCI | CCNGG | 2 cut(s) | 157, 162 |
| BstSFI | CTRYAG | 1 cut(s) | 123 |
| BstUI | CGCG | 2 cut(s) | 73, 225 |
| BstV1I | GCAGC | 1 cut(s) | 137 |
| BstX2I | RGATCY | 2 cut(s) | 166, 199 |
| BstYI | RGATCY | 2 cut(s) | 166, 199 |
| BsuRI | GGCC | 5 cut(s) | 134, 162, 248, 254, 269 |
| BtgI | CCRYGG | 1 cut(s) | 71 |
| BtsIMutI | CAGTG | 1 cut(s) | 305 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 189, 227 |
| CfoI | GCGC | 2 cut(s) | 225, 331 |
| Cfr13I | GGNCC | 2 cut(s) | 133, 253 |
| Cfr42I | CCGCGG | 1 cut(s) | 74 |
| CviJI | RGCY | 8 cut(s) | 76, 134, 162, 187, 235, 248, 254, 269 |
| CviKI_1 | RGCY | 8 cut(s) | 76, 134, 162, 187, 235, 248, 254, 269 |
| DdeI | CTNAG | 2 cut(s) | 27, 236 |
| DpnI | GATC | 3 cut(s) | 42, 168, 201 |
| DpnII | GATC | 3 cut(s) | 40, 166, 199 |
| EaeI | YGGCCR | 1 cut(s) | 267 |
| Ecl136II | GAGCTC | 1 cut(s) | 235 |
| Eco24I | GRGCYC | 1 cut(s) | 237 |
| Eco53kI | GAGCTC | 1 cut(s) | 235 |
| Eco57I | CTGAAG | 1 cut(s) | 317 |
| EcoICRI | GAGCTC | 1 cut(s) | 235 |
| EcoRII | CCWGG | 2 cut(s) | 157, 162 |
| EcoT38I | GRGCYC | 1 cut(s) | 237 |
| FaiI | YATR | 1 cut(s) | 183 |
| FaqI | GGGAC | 1 cut(s) | 92 |
| FauI | CCCGC | 2 cut(s) | 78, 117 |
| Fnu4HI | GCNGC | 2 cut(s) | 74, 126 |
| FriOI | GRGCYC | 1 cut(s) | 237 |
| Fsp4HI | GCNGC | 2 cut(s) | 74, 126 |
| GlaI | GCGC | 2 cut(s) | 224, 330 |
| GluI | GCNGC | 2 cut(s) | 74, 126 |
| GsaI | CCCAGC | 2 cut(s) | 91, 329 |
| GsuI | CTGGAG | 1 cut(s) | 329 |
| HaeIII | GGCC | 5 cut(s) | 134, 162, 248, 254, 269 |
| HhaI | GCGC | 2 cut(s) | 225, 331 |
| Hin6I | GCGC | 2 cut(s) | 223, 329 |
| HinP1I | GCGC | 2 cut(s) | 223, 329 |
| HincII | GTYRAC | 2 cut(s) | 84, 102 |
| HindII | GTYRAC | 2 cut(s) | 84, 102 |
| HpaI | GTTAAC | 2 cut(s) | 84, 102 |
| Hpy166II | GTNNAC | 2 cut(s) | 84, 102 |
| Hpy188I | TCNGA | 1 cut(s) | 239 |
| Hpy8I | GTNNAC | 2 cut(s) | 84, 102 |
| Hpy99I | CGWCG | 1 cut(s) | 293 |
| HpyAV | CCTTC | 1 cut(s) | 295 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 106 |
| HpyCH4IV | ACGT | 1 cut(s) | 80 |
| HpyCH4V | TGCA | 1 cut(s) | 125 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 131, 159, 311, 326, 337 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 236 |
| HpySE526I | ACGT | 1 cut(s) | 80 |
| HspAI | GCGC | 2 cut(s) | 223, 329 |
| KspAI | GTTAAC | 2 cut(s) | 84, 102 |
| KspI | CCGCGG | 1 cut(s) | 74 |
| Kzo9I | GATC | 3 cut(s) | 40, 166, 199 |
| LmnI | GCTCC | 2 cut(s) | 31, 220 |
| Lsp1109I | GCAGC | 1 cut(s) | 137 |
| MaeII | ACGT | 1 cut(s) | 80 |
| MalI | GATC | 3 cut(s) | 42, 168, 201 |
| MboI | GATC | 3 cut(s) | 40, 166, 199 |
| MboII | GAAGA | 2 cut(s) | 194, 310 |
| MflI | RGATCY | 2 cut(s) | 166, 199 |
| MhlI | GDGCHC | 2 cut(s) | 231, 237 |
| MlsI | TGGCCA | 1 cut(s) | 269 |
| MluCI | AATT | 1 cut(s) | 284 |
| MluNI | TGGCCA | 1 cut(s) | 269 |
| MnlI | CCTC | 4 cut(s) | 22, 124, 244, 304 |
| Mox20I | TGGCCA | 1 cut(s) | 269 |
| MscI | TGGCCA | 1 cut(s) | 269 |
| MseI | TTAA | 3 cut(s) | 83, 95, 101 |
| MslI | CAYNNNNRTG | 1 cut(s) | 275 |
| Msp20I | TGGCCA | 1 cut(s) | 269 |
| MspA1I | CMGCKG | 4 cut(s) | 73, 91, 112, 128 |
| MspR9I | CCNGG | 2 cut(s) | 159, 164 |
| MvaI | CCWGG | 2 cut(s) | 159, 164 |
| MvnI | CGCG | 2 cut(s) | 73, 225 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 131, 159, 311, 326, 337 |
| NdeII | GATC | 3 cut(s) | 40, 166, 199 |
| NmeAIII | GCCGAG | 2 cut(s) | 199, 274 |
| PkrI | GCNGC | 2 cut(s) | 75, 127 |
| PshAI | GACNNNNGTC | 1 cut(s) | 147 |
| Psp124BI | GAGCTC | 1 cut(s) | 237 |
| Psp1406I | AACGTT | 1 cut(s) | 80 |
| Psp6I | CCWGG | 2 cut(s) | 157, 162 |
| PspFI | CCCAGC | 2 cut(s) | 87, 325 |
| PspGI | CCWGG | 2 cut(s) | 157, 162 |
| PspPI | GGNCC | 2 cut(s) | 133, 253 |
| PstI | CTGCAG | 1 cut(s) | 127 |
| PsuI | RGATCY | 2 cut(s) | 166, 199 |
| RseI | CAYNNNNRTG | 1 cut(s) | 275 |
| SacI | GAGCTC | 1 cut(s) | 237 |
| SacII | CCGCGG | 1 cut(s) | 74 |
| SaqAI | TTAA | 3 cut(s) | 83, 95, 101 |
| SatI | GCNGC | 2 cut(s) | 74, 126 |
| Sau3AI | GATC | 3 cut(s) | 40, 166, 199 |
| Sau96I | GGNCC | 2 cut(s) | 133, 253 |
| ScrFI | CCNGG | 2 cut(s) | 159, 164 |
| SduI | GDGCHC | 2 cut(s) | 231, 237 |
| SetI | ASST | 3 cut(s) | 83, 189, 237 |
| SfcI | CTRYAG | 1 cut(s) | 123 |
| Sfr303I | CCGCGG | 1 cut(s) | 74 |
| SgrBI | CCGCGG | 1 cut(s) | 74 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 275 |
| Sse9I | AATT | 1 cut(s) | 284 |
| SsiI | CCGC | 6 cut(s) | 38, 71, 73, 91, 110, 128 |
| SstI | GAGCTC | 1 cut(s) | 237 |
| StyD4I | CCNGG | 2 cut(s) | 157, 162 |
| TaaI | ACNGT | 2 cut(s) | 11, 106 |
| TaiI | ACGT | 1 cut(s) | 83 |
| TaqI | TCGA | 1 cut(s) | 288 |
| TasI | AATT | 1 cut(s) | 284 |
| TauI | GCSGC | 1 cut(s) | 76 |
| Tru1I | TTAA | 3 cut(s) | 83, 95, 101 |
| Tru9I | TTAA | 3 cut(s) | 83, 95, 101 |
| TscAI | CASTG | 1 cut(s) | 312 |
| TseI | GCWGC | 1 cut(s) | 125 |
| TspRI | CASTG | 1 cut(s) | 312 |
| XapI | RAATTY | 1 cut(s) | 284 |