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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 217 |
| AciI | CCGC | 4 cut(s) | 103, 114, 137, 188 |
| AclWI | GGATC | 2 cut(s) | 101, 114 |
| AcoI | YGGCCR | 1 cut(s) | 111 |
| AcsI | RAATTY | 1 cut(s) | 224 |
| AfeI | AGCGCT | 1 cut(s) | 246 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 311 |
| AluBI | AGCT | 2 cut(s) | 194, 235 |
| AluI | AGCT | 2 cut(s) | 194, 235 |
| AlwI | GGATC | 2 cut(s) | 101, 114 |
| Aor51HI | AGCGCT | 1 cut(s) | 246 |
| AoxI | GGCC | 3 cut(s) | 111, 296, 301 |
| ApoI | RAATTY | 1 cut(s) | 224 |
| AspLEI | GCGC | 2 cut(s) | 247, 293 |
| AspS9I | GGNCC | 2 cut(s) | 297, 302 |
| AsuC2I | CCSGG | 1 cut(s) | 300 |
| BamHI | GGATCC | 1 cut(s) | 106 |
| BccI | CCATC | 1 cut(s) | 97 |
| BcnI | CCSGG | 1 cut(s) | 300 |
| BfaI | CTAG | 1 cut(s) | 248 |
| BfoI | RGCGCY | 1 cut(s) | 248 |
| BisI | GCNGC | 1 cut(s) | 114 |
| BlsI | GCNGC | 1 cut(s) | 115 |
| Bme1390I | CCNGG | 1 cut(s) | 300 |
| BmgT120I | GGNCC | 2 cut(s) | 297, 302 |
| BmiI | GGNNCC | 1 cut(s) | 108 |
| BmrFI | CCNGG | 1 cut(s) | 300 |
| BpuMI | CCSGG | 1 cut(s) | 300 |
| BsaJI | CCNNGG | 1 cut(s) | 132 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 311 |
| Bse118I | RCCGGY | 1 cut(s) | 78 |
| BseDI | CCNNGG | 1 cut(s) | 132 |
| BseGI | GGATG | 1 cut(s) | 22 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 311 |
| BseX3I | CGGCCG | 1 cut(s) | 111 |
| Bsh1285I | CGRYCG | 2 cut(s) | 79, 114 |
| BshFI | GGCC | 3 cut(s) | 113, 298, 303 |
| BsiEI | CGRYCG | 2 cut(s) | 79, 114 |
| BsiSI | CCGG | 3 cut(s) | 79, 110, 300 |
| BslFI | GGGAC | 1 cut(s) | 328 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 311 |
| BsmFI | GGGAC | 1 cut(s) | 328 |
| BsnI | GGCC | 3 cut(s) | 113, 298, 303 |
| Bsp143I | GATC | 1 cut(s) | 106 |
| BspACI | CCGC | 4 cut(s) | 103, 114, 137, 188 |
| BspANI | GGCC | 3 cut(s) | 113, 298, 303 |
| BspLI | GGNNCC | 1 cut(s) | 108 |
| BspPI | GGATC | 2 cut(s) | 101, 114 |
| BsrFI | RCCGGY | 1 cut(s) | 78 |
| BssAI | RCCGGY | 1 cut(s) | 78 |
| BssECI | CCNNGG | 1 cut(s) | 132 |
| BssMI | GATC | 1 cut(s) | 106 |
| BssT1I | CCWWGG | 1 cut(s) | 132 |
| BstF5I | GGATG | 1 cut(s) | 22 |
| BstH2I | RGCGCY | 1 cut(s) | 248 |
| BstHHI | GCGC | 2 cut(s) | 247, 293 |
| BstKTI | GATC | 1 cut(s) | 109 |
| BstMBI | GATC | 1 cut(s) | 106 |
| BstMCI | CGRYCG | 2 cut(s) | 79, 114 |
| BstSCI | CCNGG | 1 cut(s) | 298 |
| BstX2I | RGATCY | 1 cut(s) | 106 |
| BstYI | RGATCY | 1 cut(s) | 106 |
| BstZI | CGGCCG | 1 cut(s) | 111 |
| BsuRI | GGCC | 3 cut(s) | 113, 298, 303 |
| BtsCI | GGATG | 1 cut(s) | 22 |
| CfoI | GCGC | 2 cut(s) | 247, 293 |
| Cfr10I | RCCGGY | 1 cut(s) | 78 |
| Cfr13I | GGNCC | 2 cut(s) | 297, 302 |
| CseI | GACGC | 1 cut(s) | 220 |
| CviAII | CATG | 1 cut(s) | 13 |
| CviJI | RGCY | 8 cut(s) | 88, 113, 131, 194, 235, 270, 298, 303 |
| CviKI_1 | RGCY | 8 cut(s) | 88, 113, 131, 194, 235, 270, 298, 303 |
| DpnI | GATC | 1 cut(s) | 108 |
| DpnII | GATC | 1 cut(s) | 106 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 217 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 217 |
| EaeI | YGGCCR | 1 cut(s) | 111 |
| EagI | CGGCCG | 1 cut(s) | 111 |
| EciI | GGCGGA | 2 cut(s) | 152, 203 |
| EclXI | CGGCCG | 1 cut(s) | 111 |
| Eco130I | CCWWGG | 1 cut(s) | 132 |
| Eco47III | AGCGCT | 1 cut(s) | 246 |
| Eco52I | CGGCCG | 1 cut(s) | 111 |
| EcoT14I | CCWWGG | 1 cut(s) | 132 |
| ErhI | CCWWGG | 1 cut(s) | 132 |
| FaeI | CATG | 1 cut(s) | 16 |
| FaiI | YATR | 3 cut(s) | 14, 119, 311 |
| FaqI | GGGAC | 1 cut(s) | 328 |
| FatI | CATG | 1 cut(s) | 12 |
| FauI | CCCGC | 1 cut(s) | 96 |
| Fnu4HI | GCNGC | 1 cut(s) | 114 |
| FokI | GGATG | 1 cut(s) | 9 |
| Fsp4HI | GCNGC | 1 cut(s) | 114 |
| FspBI | CTAG | 1 cut(s) | 248 |
| GlaI | GCGC | 2 cut(s) | 246, 292 |
| GluI | GCNGC | 1 cut(s) | 114 |
| HaeII | RGCGCY | 1 cut(s) | 248 |
| HaeIII | GGCC | 3 cut(s) | 113, 298, 303 |
| HapII | CCGG | 3 cut(s) | 79, 110, 300 |
| HgaI | GACGC | 1 cut(s) | 220 |
| HhaI | GCGC | 2 cut(s) | 247, 293 |
| Hin1II | CATG | 1 cut(s) | 16 |
| Hin6I | GCGC | 2 cut(s) | 245, 291 |
| HinP1I | GCGC | 2 cut(s) | 245, 291 |
| HpaII | CCGG | 3 cut(s) | 79, 110, 300 |
| Hpy166II | GTNNAC | 1 cut(s) | 278 |
| Hpy8I | GTNNAC | 1 cut(s) | 278 |
| Hpy99I | CGWCG | 1 cut(s) | 214 |
| HpyAV | CCTTC | 1 cut(s) | 178 |
| HpyCH4V | TGCA | 2 cut(s) | 40, 98 |
| Hsp92II | CATG | 1 cut(s) | 16 |
| HspAI | GCGC | 2 cut(s) | 245, 291 |
| Kzo9I | GATC | 1 cut(s) | 106 |
| LpnPI | CCDG | 3 cut(s) | 92, 123, 313 |
| MaeI | CTAG | 1 cut(s) | 248 |
| MalI | GATC | 1 cut(s) | 108 |
| MboI | GATC | 1 cut(s) | 106 |
| MboII | GAAGA | 1 cut(s) | 16 |
| MflI | RGATCY | 1 cut(s) | 106 |
| MluCI | AATT | 1 cut(s) | 224 |
| MseI | TTAA | 1 cut(s) | 358 |
| MspI | CCGG | 3 cut(s) | 79, 110, 300 |
| MspR9I | CCNGG | 1 cut(s) | 300 |
| NciI | CCSGG | 1 cut(s) | 300 |
| NdeII | GATC | 1 cut(s) | 106 |
| NlaIII | CATG | 1 cut(s) | 16 |
| NlaIV | GGNNCC | 1 cut(s) | 108 |
| PkrI | GCNGC | 1 cut(s) | 115 |
| PspN4I | GGNNCC | 1 cut(s) | 108 |
| PspPI | GGNCC | 2 cut(s) | 297, 302 |
| PsuI | RGATCY | 1 cut(s) | 106 |
| SaqAI | TTAA | 1 cut(s) | 358 |
| SatI | GCNGC | 1 cut(s) | 114 |
| Sau3AI | GATC | 1 cut(s) | 106 |
| Sau96I | GGNCC | 2 cut(s) | 297, 302 |
| ScrFI | CCNGG | 1 cut(s) | 300 |
| SetI | ASST | 2 cut(s) | 196, 237 |
| Sse9I | AATT | 1 cut(s) | 224 |
| SsiI | CCGC | 4 cut(s) | 103, 114, 137, 188 |
| SspMI | CTAG | 1 cut(s) | 248 |
| StyD4I | CCNGG | 1 cut(s) | 298 |
| StyI | CCWWGG | 1 cut(s) | 132 |
| TaqI | TCGA | 1 cut(s) | 75 |
| TasI | AATT | 1 cut(s) | 224 |
| TauI | GCSGC | 1 cut(s) | 116 |
| Tru1I | TTAA | 1 cut(s) | 358 |
| Tru9I | TTAA | 1 cut(s) | 358 |
| TspDTI | ATGAA | 2 cut(s) | 17, 29 |
| XapI | RAATTY | 1 cut(s) | 224 |
| XspI | CTAG | 1 cut(s) | 248 |