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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 343 |
| Acc65I | GGTACC | 2 cut(s) | 328, 355 |
| AccB1I | GGYRCC | 2 cut(s) | 328, 355 |
| AccI | GTMKAC | 1 cut(s) | 307 |
| AccII | CGCG | 1 cut(s) | 168 |
| AciI | CCGC | 6 cut(s) | 15, 38, 74, 289, 321, 359 |
| AclWI | GGATC | 2 cut(s) | 48, 117 |
| AcoI | YGGCCR | 1 cut(s) | 210 |
| AcsI | RAATTY | 1 cut(s) | 227 |
| AfaI | GTAC | 3 cut(s) | 330, 336, 357 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 151, 365 |
| AgsI | TTSAA | 2 cut(s) | 100, 232 |
| AjnI | CCWGG | 1 cut(s) | 296 |
| AluBI | AGCT | 1 cut(s) | 130 |
| AluI | AGCT | 1 cut(s) | 130 |
| Alw21I | GWGCWC | 1 cut(s) | 296 |
| AlwI | GGATC | 2 cut(s) | 48, 117 |
| AoxI | GGCC | 3 cut(s) | 189, 195, 210 |
| ApoI | RAATTY | 1 cut(s) | 227 |
| Asp718I | GGTACC | 2 cut(s) | 328, 355 |
| AspLEI | GCGC | 2 cut(s) | 168, 180 |
| AspS9I | GGNCC | 1 cut(s) | 196 |
| AsuC2I | CCSGG | 1 cut(s) | 107 |
| BalI | TGGCCA | 1 cut(s) | 212 |
| BanI | GGYRCC | 2 cut(s) | 328, 355 |
| Bbv12I | GWGCWC | 1 cut(s) | 296 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 1 cut(s) | 298 |
| BcnI | CCSGG | 1 cut(s) | 107 |
| BfuAI | ACCTGC | 1 cut(s) | 343 |
| Bme1390I | CCNGG | 2 cut(s) | 107, 298 |
| BmgT120I | GGNCC | 1 cut(s) | 196 |
| BmiI | GGNNCC | 2 cut(s) | 330, 357 |
| BmrFI | CCNGG | 2 cut(s) | 107, 298 |
| BmsI | GCATC | 2 cut(s) | 244, 339 |
| BpuMI | CCSGG | 1 cut(s) | 107 |
| BsaJI | CCNNGG | 2 cut(s) | 105, 192 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 151, 365 |
| Bse1I | ACTGG | 1 cut(s) | 255 |
| BseBI | CCWGG | 1 cut(s) | 298 |
| BseDI | CCNNGG | 2 cut(s) | 105, 192 |
| BseGI | GGATG | 2 cut(s) | 58, 259 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 151, 365 |
| BseNI | ACTGG | 1 cut(s) | 255 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseYI | CCCAGC | 1 cut(s) | 70 |
| Bsh1236I | CGCG | 1 cut(s) | 168 |
| BshFI | GGCC | 3 cut(s) | 191, 197, 212 |
| BshNI | GGYRCC | 2 cut(s) | 328, 355 |
| BsiHKAI | GWGCWC | 1 cut(s) | 296 |
| BsiSI | CCGG | 1 cut(s) | 107 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 151, 365 |
| BsnI | GGCC | 3 cut(s) | 191, 197, 212 |
| Bsp1286I | GDGCHC | 1 cut(s) | 296 |
| Bsp143I | GATC | 2 cut(s) | 40, 109 |
| BspACI | CCGC | 6 cut(s) | 15, 38, 74, 289, 321, 359 |
| BspANI | GGCC | 3 cut(s) | 191, 197, 212 |
| BspFNI | CGCG | 1 cut(s) | 168 |
| BspLI | GGNNCC | 2 cut(s) | 330, 357 |
| BspMI | ACCTGC | 1 cut(s) | 343 |
| BspPI | GGATC | 2 cut(s) | 48, 117 |
| BspT107I | GGYRCC | 2 cut(s) | 328, 355 |
| BsrI | ACTGG | 1 cut(s) | 255 |
| BssECI | CCNNGG | 2 cut(s) | 105, 192 |
| BssMI | GATC | 2 cut(s) | 40, 109 |
| Bst2UI | CCWGG | 1 cut(s) | 298 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 254 |
| BstC8I | GCNNGC | 1 cut(s) | 36 |
| BstDEI | CTNAG | 1 cut(s) | 302 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 149 |
| BstF5I | GGATG | 2 cut(s) | 58, 259 |
| BstFNI | CGCG | 1 cut(s) | 168 |
| BstHHI | GCGC | 2 cut(s) | 168, 180 |
| BstKTI | GATC | 2 cut(s) | 43, 112 |
| BstMBI | GATC | 2 cut(s) | 40, 109 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 254, 280, 358 |
| BstNI | CCWGG | 1 cut(s) | 298 |
| BstSCI | CCNGG | 2 cut(s) | 105, 296 |
| BstUI | CGCG | 1 cut(s) | 168 |
| BstX2I | RGATCY | 1 cut(s) | 109 |
| BstYI | RGATCY | 1 cut(s) | 109 |
| BsuRI | GGCC | 3 cut(s) | 191, 197, 212 |
| BtsCI | GGATG | 2 cut(s) | 58, 259 |
| BtsIMutI | CAGTG | 1 cut(s) | 248 |
| BveI | ACCTGC | 1 cut(s) | 343 |
| Cac8I | GCNNGC | 1 cut(s) | 36 |
| CfoI | GCGC | 2 cut(s) | 168, 180 |
| Cfr13I | GGNCC | 1 cut(s) | 196 |
| Csp6I | GTAC | 3 cut(s) | 329, 335, 356 |
| CviAII | CATG | 1 cut(s) | 318 |
| CviJI | RGCY | 5 cut(s) | 130, 191, 197, 212, 301 |
| CviKI_1 | RGCY | 5 cut(s) | 130, 191, 197, 212, 301 |
| CviQI | GTAC | 3 cut(s) | 329, 335, 356 |
| DdeI | CTNAG | 1 cut(s) | 302 |
| DpnI | GATC | 2 cut(s) | 42, 111 |
| DpnII | GATC | 2 cut(s) | 40, 109 |
| EaeI | YGGCCR | 1 cut(s) | 210 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 149 |
| EcoRII | CCWGG | 1 cut(s) | 296 |
| FaeI | CATG | 1 cut(s) | 321 |
| FaiI | YATR | 5 cut(s) | 122, 124, 126, 319, 344 |
| FatI | CATG | 1 cut(s) | 317 |
| FauI | CCCGC | 1 cut(s) | 8 |
| FblI | GTMKAC | 1 cut(s) | 307 |
| FokI | GGATG | 2 cut(s) | 45, 266 |
| GlaI | GCGC | 2 cut(s) | 167, 179 |
| GsaI | CCCAGC | 1 cut(s) | 74 |
| HaeIII | GGCC | 3 cut(s) | 191, 197, 212 |
| HapII | CCGG | 1 cut(s) | 107 |
| HhaI | GCGC | 2 cut(s) | 168, 180 |
| Hin1II | CATG | 1 cut(s) | 321 |
| Hin6I | GCGC | 2 cut(s) | 166, 178 |
| HinP1I | GCGC | 2 cut(s) | 166, 178 |
| HincII | GTYRAC | 2 cut(s) | 79, 308 |
| HindII | GTYRAC | 2 cut(s) | 79, 308 |
| HpaI | GTTAAC | 1 cut(s) | 79 |
| HpaII | CCGG | 1 cut(s) | 107 |
| Hpy166II | GTNNAC | 2 cut(s) | 79, 308 |
| Hpy8I | GTNNAC | 2 cut(s) | 79, 308 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4V | TGCA | 2 cut(s) | 257, 352 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 254, 280, 358 |
| HpyF3I | CTNAG | 1 cut(s) | 302 |
| Hsp92II | CATG | 1 cut(s) | 321 |
| HspAI | GCGC | 2 cut(s) | 166, 178 |
| KpnI | GGTACC | 2 cut(s) | 332, 359 |
| KspAI | GTTAAC | 1 cut(s) | 79 |
| Kzo9I | GATC | 2 cut(s) | 40, 109 |
| LmnI | GCTCC | 3 cut(s) | 31, 163, 291 |
| LpnPI | CCDG | 7 cut(s) | 84, 116, 120, 236, 283, 310, 338 |
| LweI | GCATC | 2 cut(s) | 244, 339 |
| MaeIII | GTNAC | 1 cut(s) | 368 |
| MalI | GATC | 2 cut(s) | 42, 111 |
| MboI | GATC | 2 cut(s) | 40, 109 |
| MfeI | CAATTG | 1 cut(s) | 284 |
| MflI | RGATCY | 1 cut(s) | 109 |
| MhlI | GDGCHC | 1 cut(s) | 296 |
| MlsI | TGGCCA | 1 cut(s) | 212 |
| MluCI | AATT | 3 cut(s) | 134, 227, 284 |
| MluNI | TGGCCA | 1 cut(s) | 212 |
| MnlI | CCTC | 4 cut(s) | 22, 127, 155, 187 |
| Mox20I | TGGCCA | 1 cut(s) | 212 |
| MscI | TGGCCA | 1 cut(s) | 212 |
| MseI | TTAA | 2 cut(s) | 78, 84 |
| MslI | CAYNNNNRTG | 2 cut(s) | 218, 347 |
| Msp20I | TGGCCA | 1 cut(s) | 212 |
| MspA1I | CMGCKG | 1 cut(s) | 74 |
| MspI | CCGG | 1 cut(s) | 107 |
| MspR9I | CCNGG | 2 cut(s) | 107, 298 |
| MunI | CAATTG | 1 cut(s) | 284 |
| MvaI | CCWGG | 1 cut(s) | 298 |
| MvnI | CGCG | 1 cut(s) | 168 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 254, 280, 358 |
| NciI | CCSGG | 1 cut(s) | 107 |
| NdeII | GATC | 2 cut(s) | 40, 109 |
| NlaIII | CATG | 1 cut(s) | 321 |
| NlaIV | GGNNCC | 2 cut(s) | 330, 357 |
| NmeAIII | GCCGAG | 1 cut(s) | 217 |
| Psp6I | CCWGG | 1 cut(s) | 296 |
| PspFI | CCCAGC | 1 cut(s) | 70 |
| PspGI | CCWGG | 1 cut(s) | 296 |
| PspN4I | GGNNCC | 2 cut(s) | 330, 357 |
| PspPI | GGNCC | 1 cut(s) | 196 |
| PsuI | RGATCY | 1 cut(s) | 109 |
| RsaI | GTAC | 3 cut(s) | 330, 336, 357 |
| RsaNI | GTAC | 3 cut(s) | 329, 335, 356 |
| RseI | CAYNNNNRTG | 2 cut(s) | 218, 347 |
| SalI | GTCGAC | 1 cut(s) | 306 |
| SaqAI | TTAA | 2 cut(s) | 78, 84 |
| Sau3AI | GATC | 2 cut(s) | 40, 109 |
| Sau96I | GGNCC | 1 cut(s) | 196 |
| ScrFI | CCNGG | 2 cut(s) | 107, 298 |
| SduI | GDGCHC | 1 cut(s) | 296 |
| SetI | ASST | 3 cut(s) | 132, 147, 357 |
| SfaNI | GCATC | 2 cut(s) | 244, 339 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 218, 347 |
| Sse9I | AATT | 3 cut(s) | 134, 227, 284 |
| SsiI | CCGC | 6 cut(s) | 15, 38, 74, 289, 321, 359 |
| StyD4I | CCNGG | 2 cut(s) | 105, 296 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaqI | TCGA | 1 cut(s) | 307 |
| TasI | AATT | 3 cut(s) | 134, 227, 284 |
| Tru1I | TTAA | 2 cut(s) | 78, 84 |
| Tru9I | TTAA | 2 cut(s) | 78, 84 |
| TscAI | CASTG | 1 cut(s) | 255 |
| TspRI | CASTG | 1 cut(s) | 255 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 149 |
| XapI | RAATTY | 1 cut(s) | 227 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 155 |
| XmiI | GTMKAC | 1 cut(s) | 307 |