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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 223 |
| Acc65I | GGTACC | 2 cut(s) | 208, 235 |
| AccB1I | GGYRCC | 3 cut(s) | 194, 208, 235 |
| AccBSI | CCGCTC | 1 cut(s) | 333 |
| AclWI | GGATC | 1 cut(s) | 30 |
| AcuI | CTGAAG | 1 cut(s) | 140 |
| AfaI | GTAC | 5 cut(s) | 5, 202, 210, 237, 388 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 197, 337 |
| AgeI | ACCGGT | 1 cut(s) | 197 |
| AjnI | CCWGG | 1 cut(s) | 176 |
| AluBI | AGCT | 1 cut(s) | 289 |
| AluI | AGCT | 1 cut(s) | 289 |
| Alw21I | GWGCWC | 1 cut(s) | 353 |
| AlwI | GGATC | 1 cut(s) | 30 |
| AsiGI | ACCGGT | 1 cut(s) | 197 |
| Asp718I | GGTACC | 2 cut(s) | 208, 235 |
| AspLEI | GCGC | 3 cut(s) | 60, 154, 373 |
| AspS9I | GGNCC | 1 cut(s) | 269 |
| AsuC2I | CCSGG | 1 cut(s) | 192 |
| AvaII | GGWCC | 1 cut(s) | 269 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 192, 192, 225, 225 |
| BanI | GGYRCC | 3 cut(s) | 194, 208, 235 |
| Bbv12I | GWGCWC | 1 cut(s) | 353 |
| BbvCI | CCTCAGC | 1 cut(s) | 72 |
| BccI | CCATC | 2 cut(s) | 259, 287 |
| BciT130I | CCWGG | 1 cut(s) | 178 |
| BcnI | CCSGG | 1 cut(s) | 192 |
| BfaI | CTAG | 4 cut(s) | 11, 98, 131, 342 |
| BfmI | CTRYAG | 1 cut(s) | 354 |
| BfuAI | ACCTGC | 1 cut(s) | 223 |
| BisI | GCNGC | 1 cut(s) | 320 |
| BlsI | GCNGC | 1 cut(s) | 321 |
| Bme1390I | CCNGG | 2 cut(s) | 178, 192 |
| Bme18I | GGWCC | 1 cut(s) | 269 |
| BmgT120I | GGNCC | 1 cut(s) | 269 |
| BmiI | GGNNCC | 5 cut(s) | 196, 210, 216, 237, 270 |
| BmrFI | CCNGG | 2 cut(s) | 178, 192 |
| BpmI | CTGGAG | 1 cut(s) | 360 |
| Bpu10I | CCTNAGC | 1 cut(s) | 72 |
| BpuMI | CCSGG | 1 cut(s) | 192 |
| BsaAI | YACGTR | 1 cut(s) | 170 |
| BsaWI | WCCGGW | 2 cut(s) | 197, 211 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 197, 337 |
| Bse118I | RCCGGY | 1 cut(s) | 197 |
| Bse1I | ACTGG | 1 cut(s) | 218 |
| BseBI | CCWGG | 1 cut(s) | 178 |
| BseGI | GGATG | 2 cut(s) | 270, 279 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 197, 337 |
| BseMII | CTCAG | 1 cut(s) | 63 |
| BseNI | ACTGG | 1 cut(s) | 218 |
| BseRI | GAGGAG | 2 cut(s) | 65, 68 |
| BseYI | CCCAGC | 1 cut(s) | 285 |
| BsgI | GTGCAG | 1 cut(s) | 251 |
| BshNI | GGYRCC | 3 cut(s) | 194, 208, 235 |
| BshTI | ACCGGT | 1 cut(s) | 197 |
| BsiHKAI | GWGCWC | 1 cut(s) | 353 |
| BsiSI | CCGG | 3 cut(s) | 192, 198, 212 |
| BslFI | GGGAC | 1 cut(s) | 282 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 197, 337 |
| BsmFI | GGGAC | 1 cut(s) | 282 |
| Bsp1286I | GDGCHC | 1 cut(s) | 353 |
| Bsp1407I | TGTACA | 1 cut(s) | 386 |
| Bsp143I | GATC | 1 cut(s) | 22 |
| BspCNI | CTCAG | 1 cut(s) | 64 |
| BspLI | GGNNCC | 5 cut(s) | 196, 210, 216, 237, 270 |
| BspMAI | CTGCAG | 1 cut(s) | 358 |
| BspMI | ACCTGC | 1 cut(s) | 223 |
| BspPI | GGATC | 1 cut(s) | 30 |
| BspT107I | GGYRCC | 3 cut(s) | 194, 208, 235 |
| BsrBI | CCGCTC | 1 cut(s) | 333 |
| BsrFI | RCCGGY | 1 cut(s) | 197 |
| BsrGI | TGTACA | 1 cut(s) | 386 |
| BsrI | ACTGG | 1 cut(s) | 218 |
| BssAI | RCCGGY | 1 cut(s) | 197 |
| BssMI | GATC | 1 cut(s) | 22 |
| Bst2UI | CCWGG | 1 cut(s) | 178 |
| Bst4CI | ACNGT | 2 cut(s) | 87, 165 |
| Bst6I | CTCTTC | 1 cut(s) | 116 |
| BstAUI | TGTACA | 1 cut(s) | 386 |
| BstBAI | YACGTR | 1 cut(s) | 170 |
| BstC8I | GCNNGC | 2 cut(s) | 358, 369 |
| BstDEI | CTNAG | 1 cut(s) | 72 |
| BstF5I | GGATG | 2 cut(s) | 270, 279 |
| BstHHI | GCGC | 3 cut(s) | 60, 154, 373 |
| BstKTI | GATC | 1 cut(s) | 25 |
| BstMBI | GATC | 1 cut(s) | 22 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 149, 238, 357, 377 |
| BstNI | CCWGG | 1 cut(s) | 178 |
| BstSCI | CCNGG | 2 cut(s) | 176, 190 |
| BstSFI | CTRYAG | 1 cut(s) | 354 |
| BstX2I | RGATCY | 1 cut(s) | 22 |
| BstYI | RGATCY | 1 cut(s) | 22 |
| BtsCI | GGATG | 2 cut(s) | 270, 279 |
| BtsI | GCAGTG | 2 cut(s) | 314, 351 |
| BtsIMutI | CAGTG | 2 cut(s) | 314, 351 |
| BveI | ACCTGC | 1 cut(s) | 223 |
| Cac8I | GCNNGC | 2 cut(s) | 358, 369 |
| CfoI | GCGC | 3 cut(s) | 60, 154, 373 |
| Cfr10I | RCCGGY | 1 cut(s) | 197 |
| Cfr13I | GGNCC | 1 cut(s) | 269 |
| CseI | GACGC | 2 cut(s) | 136, 254 |
| Csp6I | GTAC | 5 cut(s) | 4, 201, 209, 236, 387 |
| CspAI | ACCGGT | 1 cut(s) | 197 |
| CviJI | RGCY | 8 cut(s) | 71, 77, 92, 137, 217, 289, 341, 393 |
| CviKI_1 | RGCY | 8 cut(s) | 71, 77, 92, 137, 217, 289, 341, 393 |
| CviQI | GTAC | 5 cut(s) | 4, 201, 209, 236, 387 |
| DdeI | CTNAG | 1 cut(s) | 72 |
| DpnI | GATC | 1 cut(s) | 24 |
| DpnII | GATC | 1 cut(s) | 22 |
| Eam1104I | CTCTTC | 1 cut(s) | 116 |
| EarI | CTCTTC | 1 cut(s) | 116 |
| EciI | GGCGGA | 3 cut(s) | 35, 323, 354 |
| Eco47I | GGWCC | 1 cut(s) | 269 |
| Eco57I | CTGAAG | 1 cut(s) | 140 |
| EcoRII | CCWGG | 1 cut(s) | 176 |
| FaiI | YATR | 3 cut(s) | 30, 224, 324 |
| FaqI | GGGAC | 1 cut(s) | 282 |
| Fnu4HI | GCNGC | 1 cut(s) | 320 |
| FokI | GGATG | 2 cut(s) | 266, 277 |
| Fsp4HI | GCNGC | 1 cut(s) | 320 |
| FspBI | CTAG | 4 cut(s) | 11, 98, 131, 342 |
| GlaI | GCGC | 3 cut(s) | 59, 153, 372 |
| GluI | GCNGC | 1 cut(s) | 320 |
| GsaI | CCCAGC | 1 cut(s) | 289 |
| GsuI | CTGGAG | 1 cut(s) | 360 |
| HapII | CCGG | 3 cut(s) | 192, 198, 212 |
| HgaI | GACGC | 2 cut(s) | 136, 254 |
| HhaI | GCGC | 3 cut(s) | 60, 154, 373 |
| Hin6I | GCGC | 3 cut(s) | 58, 152, 371 |
| HinP1I | GCGC | 3 cut(s) | 58, 152, 371 |
| HinfI | GANTC | 1 cut(s) | 311 |
| HpaII | CCGG | 3 cut(s) | 192, 198, 212 |
| Hpy188I | TCNGA | 1 cut(s) | 337 |
| HpyCH4III | ACNGT | 2 cut(s) | 87, 165 |
| HpyCH4IV | ACGT | 3 cut(s) | 46, 106, 169 |
| HpyCH4V | TGCA | 2 cut(s) | 232, 356 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 149, 238, 357, 377 |
| HpyF3I | CTNAG | 1 cut(s) | 72 |
| HpySE526I | ACGT | 3 cut(s) | 46, 106, 169 |
| HspAI | GCGC | 3 cut(s) | 58, 152, 371 |
| KpnI | GGTACC | 2 cut(s) | 212, 239 |
| Kzo9I | GATC | 1 cut(s) | 22 |
| LmnI | GCTCC | 3 cut(s) | 55, 214, 298 |
| MaeI | CTAG | 4 cut(s) | 11, 98, 131, 342 |
| MaeII | ACGT | 3 cut(s) | 46, 106, 169 |
| MaeIII | GTNAC | 3 cut(s) | 102, 165, 170 |
| MalI | GATC | 1 cut(s) | 24 |
| MbiI | CCGCTC | 1 cut(s) | 333 |
| MboI | GATC | 1 cut(s) | 22 |
| MboII | GAAGA | 2 cut(s) | 17, 133 |
| MflI | RGATCY | 1 cut(s) | 22 |
| MhlI | GDGCHC | 1 cut(s) | 353 |
| MluCI | AATT | 1 cut(s) | 254 |
| MmeI | TCCRAC | 1 cut(s) | 276 |
| MnlI | CCTC | 8 cut(s) | 43, 46, 67, 117, 127, 298, 331, 384 |
| MslI | CAYNNNNRTG | 1 cut(s) | 227 |
| MspA1I | CMGCKG | 2 cut(s) | 241, 380 |
| MspI | CCGG | 3 cut(s) | 192, 198, 212 |
| MspR9I | CCNGG | 2 cut(s) | 178, 192 |
| MvaI | CCWGG | 1 cut(s) | 178 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 149, 238, 357, 377 |
| NciI | CCSGG | 1 cut(s) | 192 |
| NdeII | GATC | 1 cut(s) | 22 |
| NlaIV | GGNNCC | 5 cut(s) | 196, 210, 216, 237, 270 |
| NmuCI | GTSAC | 2 cut(s) | 102, 165 |
| PfeI | GAWTC | 1 cut(s) | 311 |
| PinAI | ACCGGT | 1 cut(s) | 197 |
| PkrI | GCNGC | 1 cut(s) | 321 |
| Ppu21I | YACGTR | 1 cut(s) | 170 |
| Psp6I | CCWGG | 1 cut(s) | 176 |
| PspFI | CCCAGC | 1 cut(s) | 285 |
| PspGI | CCWGG | 1 cut(s) | 176 |
| PspN4I | GGNNCC | 5 cut(s) | 196, 210, 216, 237, 270 |
| PspPI | GGNCC | 1 cut(s) | 269 |
| PstI | CTGCAG | 1 cut(s) | 358 |
| PsuI | RGATCY | 1 cut(s) | 22 |
| RsaI | GTAC | 5 cut(s) | 5, 202, 210, 237, 388 |
| RsaNI | GTAC | 5 cut(s) | 4, 201, 209, 236, 387 |
| RseI | CAYNNNNRTG | 1 cut(s) | 227 |
| SatI | GCNGC | 1 cut(s) | 320 |
| Sau3AI | GATC | 1 cut(s) | 22 |
| Sau96I | GGNCC | 1 cut(s) | 269 |
| ScrFI | CCNGG | 2 cut(s) | 178, 192 |
| SduI | GDGCHC | 1 cut(s) | 353 |
| SetI | ASST | 7 cut(s) | 49, 109, 172, 231, 237, 253, 291 |
| SfcI | CTRYAG | 1 cut(s) | 354 |
| SinI | GGWCC | 1 cut(s) | 269 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 227 |
| Sse9I | AATT | 1 cut(s) | 254 |
| SspMI | CTAG | 4 cut(s) | 11, 98, 131, 342 |
| StyD4I | CCNGG | 2 cut(s) | 176, 190 |
| TaaI | ACNGT | 2 cut(s) | 87, 165 |
| TaiI | ACGT | 3 cut(s) | 49, 109, 172 |
| TaqI | TCGA | 1 cut(s) | 187 |
| TasI | AATT | 1 cut(s) | 254 |
| TatI | WGTACW | 1 cut(s) | 386 |
| TauI | GCSGC | 1 cut(s) | 322 |
| TfiI | GAWTC | 1 cut(s) | 311 |
| TscAI | CASTG | 2 cut(s) | 321, 358 |
| TseFI | GTSAC | 2 cut(s) | 102, 165 |
| Tsp45I | GTSAC | 2 cut(s) | 102, 165 |
| TspDTI | ATGAA | 2 cut(s) | 17, 129 |
| TspRI | CASTG | 2 cut(s) | 321, 358 |
| VpaK11BI | GGWCC | 1 cut(s) | 269 |
| XspI | CTAG | 4 cut(s) | 11, 98, 131, 342 |