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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 146 |
| Acc36I | ACCTGC | 2 cut(s) | 146, 293 |
| AciI | CCGC | 5 cut(s) | 19, 43, 90, 211, 342 |
| AclWI | GGATC | 1 cut(s) | 100 |
| AcoI | YGGCCR | 2 cut(s) | 44, 160 |
| AfaI | GTAC | 1 cut(s) | 266 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 155, 267 |
| AjnI | CCWGG | 1 cut(s) | 246 |
| AleI | CACNNNNGTG | 1 cut(s) | 168 |
| AluBI | AGCT | 3 cut(s) | 80, 326, 359 |
| AluI | AGCT | 3 cut(s) | 80, 326, 359 |
| Alw21I | GWGCWC | 1 cut(s) | 246 |
| Alw26I | GTCTC | 2 cut(s) | 18, 311 |
| AlwI | GGATC | 1 cut(s) | 100 |
| Ama87I | CYCGRG | 1 cut(s) | 32 |
| AoxI | GGCC | 5 cut(s) | 44, 53, 145, 160, 339 |
| ApeKI | GCWGC | 1 cut(s) | 40 |
| AspLEI | GCGC | 2 cut(s) | 130, 273 |
| AspS9I | GGNCC | 2 cut(s) | 146, 339 |
| AsuC2I | CCSGG | 5 cut(s) | 6, 16, 33, 34, 219 |
| AsuHPI | GGTGA | 2 cut(s) | 33, 56 |
| AvaI | CYCGRG | 1 cut(s) | 32 |
| BaeGI | GKGCMC | 1 cut(s) | 355 |
| BalI | TGGCCA | 1 cut(s) | 162 |
| Bbv12I | GWGCWC | 1 cut(s) | 246 |
| BbvI | GCAGC | 1 cut(s) | 52 |
| BciT130I | CCWGG | 1 cut(s) | 248 |
| BcnI | CCSGG | 5 cut(s) | 6, 16, 33, 34, 219 |
| BcoDI | GTCTC | 2 cut(s) | 18, 311 |
| BfaI | CTAG | 1 cut(s) | 262 |
| BfuAI | ACCTGC | 2 cut(s) | 146, 293 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 52, 219 |
| BisI | GCNGC | 3 cut(s) | 41, 44, 342 |
| BlsI | GCNGC | 3 cut(s) | 42, 45, 343 |
| Bme1390I | CCNGG | 6 cut(s) | 6, 16, 33, 34, 219, 248 |
| BmeT110I | CYCGRG | 1 cut(s) | 32 |
| BmgT120I | GGNCC | 2 cut(s) | 146, 339 |
| BmiI | GGNNCC | 1 cut(s) | 340 |
| BmrFI | CCNGG | 6 cut(s) | 6, 16, 33, 34, 219, 248 |
| BpmI | CTGGAG | 1 cut(s) | 222 |
| BpuMI | CCSGG | 5 cut(s) | 6, 16, 33, 34, 219 |
| BsaI | GGTCTC | 1 cut(s) | 311 |
| BsaJI | CCNNGG | 4 cut(s) | 4, 14, 32, 142 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 155, 267 |
| Bse118I | RCCGGY | 1 cut(s) | 267 |
| Bse1I | ACTGG | 2 cut(s) | 91, 288 |
| BseBI | CCWGG | 1 cut(s) | 248 |
| BseDI | CCNNGG | 4 cut(s) | 4, 14, 32, 142 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 155, 267 |
| BseNI | ACTGG | 2 cut(s) | 91, 288 |
| BseSI | GKGCMC | 1 cut(s) | 355 |
| BseXI | GCAGC | 1 cut(s) | 52 |
| BseYI | CCCAGC | 1 cut(s) | 355 |
| BshFI | GGCC | 5 cut(s) | 46, 55, 147, 162, 341 |
| BsiHKAI | GWGCWC | 1 cut(s) | 246 |
| BsiHKCI | CYCGRG | 1 cut(s) | 32 |
| BsiSI | CCGG | 5 cut(s) | 6, 16, 33, 219, 268 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 155, 267 |
| BsmAI | GTCTC | 2 cut(s) | 18, 311 |
| BsnI | GGCC | 5 cut(s) | 46, 55, 147, 162, 341 |
| Bso31I | GGTCTC | 1 cut(s) | 311 |
| BsoBI | CYCGRG | 1 cut(s) | 32 |
| Bsp1286I | GDGCHC | 2 cut(s) | 246, 355 |
| Bsp143I | GATC | 1 cut(s) | 92 |
| BspACI | CCGC | 5 cut(s) | 19, 43, 90, 211, 342 |
| BspANI | GGCC | 5 cut(s) | 46, 55, 147, 162, 341 |
| BspLI | GGNNCC | 1 cut(s) | 340 |
| BspMI | ACCTGC | 2 cut(s) | 146, 293 |
| BspPI | GGATC | 1 cut(s) | 100 |
| BspTNI | GGTCTC | 1 cut(s) | 311 |
| BsrFI | RCCGGY | 1 cut(s) | 267 |
| BsrI | ACTGG | 2 cut(s) | 91, 288 |
| BssAI | RCCGGY | 1 cut(s) | 267 |
| BssECI | CCNNGG | 4 cut(s) | 4, 14, 32, 142 |
| BssMI | GATC | 1 cut(s) | 92 |
| Bst2UI | CCWGG | 1 cut(s) | 248 |
| BstC8I | GCNNGC | 1 cut(s) | 82 |
| BstHHI | GCGC | 2 cut(s) | 130, 273 |
| BstKTI | GATC | 1 cut(s) | 95 |
| BstMAI | GTCTC | 2 cut(s) | 18, 311 |
| BstMBI | GATC | 1 cut(s) | 92 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 52, 219, 308, 350 |
| BstNI | CCWGG | 1 cut(s) | 248 |
| BstSCI | CCNGG | 6 cut(s) | 4, 14, 31, 32, 217, 246 |
| BstSLI | GKGCMC | 1 cut(s) | 355 |
| BstV1I | GCAGC | 1 cut(s) | 52 |
| BstX2I | RGATCY | 1 cut(s) | 92 |
| BstYI | RGATCY | 1 cut(s) | 92 |
| BsuRI | GGCC | 5 cut(s) | 46, 55, 147, 162, 341 |
| BtsIMutI | CAGTG | 1 cut(s) | 84 |
| BveI | ACCTGC | 2 cut(s) | 146, 293 |
| Cac8I | GCNNGC | 1 cut(s) | 82 |
| CfoI | GCGC | 2 cut(s) | 130, 273 |
| Cfr10I | RCCGGY | 1 cut(s) | 267 |
| Cfr13I | GGNCC | 2 cut(s) | 146, 339 |
| Cfr9I | CCCGGG | 1 cut(s) | 32 |
| Csp6I | GTAC | 1 cut(s) | 265 |
| CviQI | GTAC | 1 cut(s) | 265 |
| DpnI | GATC | 1 cut(s) | 94 |
| DpnII | GATC | 1 cut(s) | 92 |
| EaeI | YGGCCR | 2 cut(s) | 44, 160 |
| EciI | GGCGGA | 1 cut(s) | 105 |
| Eco31I | GGTCTC | 1 cut(s) | 311 |
| Eco88I | CYCGRG | 1 cut(s) | 32 |
| EcoRII | CCWGG | 1 cut(s) | 246 |
| FaiI | YATR | 4 cut(s) | 74, 76, 298, 300 |
| FauNDI | CATATG | 1 cut(s) | 298 |
| Fnu4HI | GCNGC | 3 cut(s) | 41, 44, 342 |
| Fsp4HI | GCNGC | 3 cut(s) | 41, 44, 342 |
| FspBI | CTAG | 1 cut(s) | 262 |
| GlaI | GCGC | 2 cut(s) | 129, 272 |
| GluI | GCNGC | 3 cut(s) | 41, 44, 342 |
| GsaI | CCCAGC | 1 cut(s) | 359 |
| GsuI | CTGGAG | 1 cut(s) | 222 |
| HaeIII | GGCC | 5 cut(s) | 46, 55, 147, 162, 341 |
| HapII | CCGG | 5 cut(s) | 6, 16, 33, 219, 268 |
| HhaI | GCGC | 2 cut(s) | 130, 273 |
| Hin6I | GCGC | 2 cut(s) | 128, 271 |
| HinP1I | GCGC | 2 cut(s) | 128, 271 |
| HpaII | CCGG | 5 cut(s) | 6, 16, 33, 219, 268 |
| HphI | GGTGA | 2 cut(s) | 33, 56 |
| Hpy166II | GTNNAC | 2 cut(s) | 11, 120 |
| Hpy188III | TCNNGA | 1 cut(s) | 331 |
| Hpy8I | GTNNAC | 2 cut(s) | 11, 120 |
| HpyCH4IV | ACGT | 2 cut(s) | 116, 176 |
| HpyCH4V | TGCA | 2 cut(s) | 296, 302 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 52, 219, 308, 350 |
| HpySE526I | ACGT | 2 cut(s) | 116, 176 |
| HspAI | GCGC | 2 cut(s) | 128, 271 |
| Kzo9I | GATC | 1 cut(s) | 92 |
| LmnI | GCTCC | 1 cut(s) | 368 |
| Lsp1109I | GCAGC | 1 cut(s) | 52 |
| MaeI | CTAG | 1 cut(s) | 262 |
| MaeII | ACGT | 2 cut(s) | 116, 176 |
| MaeIII | GTNAC | 1 cut(s) | 172 |
| MalI | GATC | 1 cut(s) | 94 |
| MboI | GATC | 1 cut(s) | 92 |
| MboII | GAAGA | 1 cut(s) | 87 |
| MflI | RGATCY | 1 cut(s) | 92 |
| MhlI | GDGCHC | 2 cut(s) | 246, 355 |
| MlsI | TGGCCA | 1 cut(s) | 162 |
| MluNI | TGGCCA | 1 cut(s) | 162 |
| MmeI | TCCRAC | 1 cut(s) | 346 |
| MnlI | CCTC | 4 cut(s) | 77, 137, 197, 368 |
| Mox20I | TGGCCA | 1 cut(s) | 162 |
| MscI | TGGCCA | 1 cut(s) | 162 |
| MslI | CAYNNNNRTG | 1 cut(s) | 168 |
| Msp20I | TGGCCA | 1 cut(s) | 162 |
| MspA1I | CMGCKG | 1 cut(s) | 43 |
| MspI | CCGG | 5 cut(s) | 6, 16, 33, 219, 268 |
| MspR9I | CCNGG | 6 cut(s) | 6, 16, 33, 34, 219, 248 |
| MvaI | CCWGG | 1 cut(s) | 248 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 52, 219, 308, 350 |
| NciI | CCSGG | 5 cut(s) | 6, 16, 33, 34, 219 |
| NdeI | CATATG | 1 cut(s) | 298 |
| NdeII | GATC | 1 cut(s) | 92 |
| NlaIV | GGNNCC | 1 cut(s) | 340 |
| NmeAIII | GCCGAG | 1 cut(s) | 167 |
| NmuCI | GTSAC | 1 cut(s) | 172 |
| OliI | CACNNNNGTG | 1 cut(s) | 168 |
| PaqCI | CACCTGC | 1 cut(s) | 146 |
| PkrI | GCNGC | 3 cut(s) | 42, 45, 343 |
| Psp6I | CCWGG | 1 cut(s) | 246 |
| PspFI | CCCAGC | 1 cut(s) | 355 |
| PspGI | CCWGG | 1 cut(s) | 246 |
| PspN4I | GGNNCC | 1 cut(s) | 340 |
| PspPI | GGNCC | 2 cut(s) | 146, 339 |
| PsuI | RGATCY | 1 cut(s) | 92 |
| RsaI | GTAC | 1 cut(s) | 266 |
| RsaNI | GTAC | 1 cut(s) | 265 |
| RseI | CAYNNNNRTG | 1 cut(s) | 168 |
| SatI | GCNGC | 3 cut(s) | 41, 44, 342 |
| Sau3AI | GATC | 1 cut(s) | 92 |
| Sau96I | GGNCC | 2 cut(s) | 146, 339 |
| ScrFI | CCNGG | 6 cut(s) | 6, 16, 33, 34, 219, 248 |
| SduI | GDGCHC | 2 cut(s) | 246, 355 |
| SetI | ASST | 9 cut(s) | 69, 82, 119, 160, 179, 263, 307, 328, 361 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 52 |
| SmaI | CCCGGG | 1 cut(s) | 34 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 168 |
| SsiI | CCGC | 5 cut(s) | 19, 43, 90, 211, 342 |
| SspMI | CTAG | 1 cut(s) | 262 |
| StyD4I | CCNGG | 6 cut(s) | 4, 14, 31, 32, 217, 246 |
| TaiI | ACGT | 2 cut(s) | 119, 179 |
| TauI | GCSGC | 2 cut(s) | 46, 344 |
| TscAI | CASTG | 1 cut(s) | 91 |
| TseFI | GTSAC | 1 cut(s) | 172 |
| TseI | GCWGC | 1 cut(s) | 40 |
| Tsp45I | GTSAC | 1 cut(s) | 172 |
| TspDTI | ATGAA | 1 cut(s) | 199 |
| TspGWI | ACGGA | 1 cut(s) | 239 |
| TspMI | CCCGGG | 1 cut(s) | 32 |
| TspRI | CASTG | 1 cut(s) | 91 |
| XmaI | CCCGGG | 1 cut(s) | 32 |
| XspI | CTAG | 1 cut(s) | 262 |