No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 298 |
| AccB1I | GGYRCC | 2 cut(s) | 284, 298 |
| AccI | GTMKAC | 1 cut(s) | 277 |
| AciI | CCGC | 6 cut(s) | 21, 39, 64, 68, 110, 235 |
| AclWI | GGATC | 1 cut(s) | 120 |
| AfaI | GTAC | 3 cut(s) | 19, 95, 300 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 70, 287 |
| AflIII | ACRYGT | 1 cut(s) | 194 |
| AluBI | AGCT | 3 cut(s) | 100, 182, 346 |
| AluI | AGCT | 3 cut(s) | 100, 182, 346 |
| AlwI | GGATC | 1 cut(s) | 120 |
| AoxI | GGCC | 4 cut(s) | 4, 73, 165, 225 |
| ApeKI | GCWGC | 2 cut(s) | 57, 60 |
| Asp718I | GGTACC | 1 cut(s) | 298 |
| AspLEI | GCGC | 1 cut(s) | 150 |
| AspS9I | GGNCC | 3 cut(s) | 4, 73, 166 |
| AsuC2I | CCSGG | 3 cut(s) | 36, 72, 282 |
| AsuHPI | GGTGA | 1 cut(s) | 53 |
| AsuNHI | GCTAGC | 1 cut(s) | 100 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 282, 315 |
| BanI | GGYRCC | 2 cut(s) | 284, 298 |
| BbvCI | CCTCAGC | 1 cut(s) | 162 |
| BbvI | GCAGC | 2 cut(s) | 69, 72 |
| BcnI | CCSGG | 3 cut(s) | 36, 72, 282 |
| BfaI | CTAG | 3 cut(s) | 101, 221, 267 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 72 |
| BglII | AGATCT | 1 cut(s) | 79 |
| BisI | GCNGC | 3 cut(s) | 58, 61, 64 |
| BlsI | GCNGC | 3 cut(s) | 59, 62, 65 |
| Bme1390I | CCNGG | 3 cut(s) | 36, 72, 282 |
| BmgT120I | GGNCC | 3 cut(s) | 4, 73, 166 |
| BmiI | GGNNCC | 2 cut(s) | 286, 300 |
| BmrFI | CCNGG | 3 cut(s) | 36, 72, 282 |
| BmsI | GCATC | 1 cut(s) | 309 |
| BmtI | GCTAGC | 1 cut(s) | 104 |
| Bpu10I | CCTNAGC | 2 cut(s) | 162, 342 |
| BpuMI | CCSGG | 3 cut(s) | 36, 72, 282 |
| BsaAI | YACGTR | 1 cut(s) | 260 |
| BsaJI | CCNNGG | 1 cut(s) | 34 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 70, 287 |
| Bse118I | RCCGGY | 2 cut(s) | 287, 307 |
| Bse1I | ACTGG | 1 cut(s) | 111 |
| BseDI | CCNNGG | 1 cut(s) | 34 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 70, 287 |
| BseMII | CTCAG | 2 cut(s) | 153, 333 |
| BseNI | ACTGG | 1 cut(s) | 111 |
| BseXI | GCAGC | 2 cut(s) | 69, 72 |
| BseYI | CCCAGC | 1 cut(s) | 238 |
| BsgI | GTGCAG | 1 cut(s) | 335 |
| BshFI | GGCC | 4 cut(s) | 6, 75, 167, 227 |
| BshNI | GGYRCC | 2 cut(s) | 284, 298 |
| BsiSI | CCGG | 5 cut(s) | 36, 71, 282, 288, 308 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 70, 287 |
| BsnI | GGCC | 4 cut(s) | 6, 75, 167, 227 |
| Bsp143I | GATC | 2 cut(s) | 79, 112 |
| BspACI | CCGC | 6 cut(s) | 21, 39, 64, 68, 110, 235 |
| BspANI | GGCC | 4 cut(s) | 6, 75, 167, 227 |
| BspCNI | CTCAG | 2 cut(s) | 154, 334 |
| BspHI | TCATGA | 2 cut(s) | 189, 350 |
| BspLI | GGNNCC | 2 cut(s) | 286, 300 |
| BspOI | GCTAGC | 1 cut(s) | 104 |
| BspPI | GGATC | 1 cut(s) | 120 |
| BspT107I | GGYRCC | 2 cut(s) | 284, 298 |
| BsrFI | RCCGGY | 2 cut(s) | 287, 307 |
| BsrI | ACTGG | 1 cut(s) | 111 |
| BssAI | RCCGGY | 2 cut(s) | 287, 307 |
| BssECI | CCNNGG | 1 cut(s) | 34 |
| BssMI | GATC | 2 cut(s) | 79, 112 |
| BstBAI | YACGTR | 1 cut(s) | 260 |
| BstC8I | GCNNGC | 3 cut(s) | 68, 102, 251 |
| BstDEI | CTNAG | 2 cut(s) | 162, 342 |
| BstHHI | GCGC | 1 cut(s) | 150 |
| BstKTI | GATC | 2 cut(s) | 82, 115 |
| BstMBI | GATC | 2 cut(s) | 79, 112 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 63, 72, 313 |
| BstSCI | CCNGG | 3 cut(s) | 34, 70, 280 |
| BstV1I | GCAGC | 2 cut(s) | 69, 72 |
| BstX2I | RGATCY | 2 cut(s) | 79, 112 |
| BstYI | RGATCY | 2 cut(s) | 79, 112 |
| BsuRI | GGCC | 4 cut(s) | 6, 75, 167, 227 |
| BtsIMutI | CAGTG | 3 cut(s) | 104, 228, 329 |
| Cac8I | GCNNGC | 3 cut(s) | 68, 102, 251 |
| CciI | TCATGA | 2 cut(s) | 189, 350 |
| CfoI | GCGC | 1 cut(s) | 150 |
| Cfr10I | RCCGGY | 2 cut(s) | 287, 307 |
| Cfr13I | GGNCC | 3 cut(s) | 4, 73, 166 |
| Csp6I | GTAC | 3 cut(s) | 18, 94, 299 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 31 |
| CviAII | CATG | 2 cut(s) | 190, 351 |
| CviQI | GTAC | 3 cut(s) | 18, 94, 299 |
| DdeI | CTNAG | 2 cut(s) | 162, 342 |
| DpnI | GATC | 2 cut(s) | 81, 114 |
| DpnII | GATC | 2 cut(s) | 79, 112 |
| EciI | GGCGGA | 1 cut(s) | 125 |
| FaeI | CATG | 2 cut(s) | 193, 354 |
| FaiI | YATR | 4 cut(s) | 92, 120, 191, 352 |
| FatI | CATG | 2 cut(s) | 189, 350 |
| FauI | CCCGC | 1 cut(s) | 75 |
| FblI | GTMKAC | 1 cut(s) | 277 |
| Fnu4HI | GCNGC | 3 cut(s) | 58, 61, 64 |
| Fsp4HI | GCNGC | 3 cut(s) | 58, 61, 64 |
| FspBI | CTAG | 3 cut(s) | 101, 221, 267 |
| GlaI | GCGC | 1 cut(s) | 149 |
| GluI | GCNGC | 3 cut(s) | 58, 61, 64 |
| GsaI | CCCAGC | 1 cut(s) | 242 |
| HaeIII | GGCC | 4 cut(s) | 6, 75, 167, 227 |
| HapII | CCGG | 5 cut(s) | 36, 71, 282, 288, 308 |
| HhaI | GCGC | 1 cut(s) | 150 |
| Hin1II | CATG | 2 cut(s) | 193, 354 |
| Hin6I | GCGC | 1 cut(s) | 148 |
| HinP1I | GCGC | 1 cut(s) | 148 |
| HincII | GTYRAC | 1 cut(s) | 278 |
| HindII | GTYRAC | 1 cut(s) | 278 |
| HpaII | CCGG | 5 cut(s) | 36, 71, 282, 288, 308 |
| HphI | GGTGA | 1 cut(s) | 53 |
| Hpy166II | GTNNAC | 1 cut(s) | 278 |
| Hpy188III | TCNNGA | 2 cut(s) | 190, 351 |
| Hpy8I | GTNNAC | 1 cut(s) | 278 |
| HpyAV | CCTTC | 1 cut(s) | 136 |
| HpyCH4IV | ACGT | 3 cut(s) | 136, 196, 259 |
| HpyCH4V | TGCA | 2 cut(s) | 316, 322 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 63, 72, 313 |
| HpyF3I | CTNAG | 2 cut(s) | 162, 342 |
| HpySE526I | ACGT | 3 cut(s) | 136, 196, 259 |
| Hsp92II | CATG | 2 cut(s) | 193, 354 |
| HspAI | GCGC | 1 cut(s) | 148 |
| KpnI | GGTACC | 1 cut(s) | 302 |
| Kzo9I | GATC | 2 cut(s) | 79, 112 |
| LmnI | GCTCC | 1 cut(s) | 145 |
| LpnPI | CCDG | 7 cut(s) | 49, 84, 92, 252, 295, 301, 321 |
| Lsp1109I | GCAGC | 2 cut(s) | 69, 72 |
| LweI | GCATC | 1 cut(s) | 309 |
| MaeI | CTAG | 3 cut(s) | 101, 221, 267 |
| MaeII | ACGT | 3 cut(s) | 136, 196, 259 |
| MaeIII | GTNAC | 2 cut(s) | 85, 255 |
| MalI | GATC | 2 cut(s) | 81, 114 |
| MboI | GATC | 2 cut(s) | 79, 112 |
| MboII | GAAGA | 1 cut(s) | 107 |
| MflI | RGATCY | 2 cut(s) | 79, 112 |
| MnlI | CCTC | 4 cut(s) | 37, 157, 196, 217 |
| MspI | CCGG | 5 cut(s) | 36, 71, 282, 288, 308 |
| MspR9I | CCNGG | 3 cut(s) | 36, 72, 282 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 63, 72, 313 |
| NciI | CCSGG | 3 cut(s) | 36, 72, 282 |
| NdeII | GATC | 2 cut(s) | 79, 112 |
| NheI | GCTAGC | 1 cut(s) | 100 |
| NlaIII | CATG | 2 cut(s) | 193, 354 |
| NlaIV | GGNNCC | 2 cut(s) | 286, 300 |
| NmuCI | GTSAC | 2 cut(s) | 85, 255 |
| PagI | TCATGA | 2 cut(s) | 189, 350 |
| PkrI | GCNGC | 3 cut(s) | 59, 62, 65 |
| Ppu21I | YACGTR | 1 cut(s) | 260 |
| PspFI | CCCAGC | 1 cut(s) | 238 |
| PspN4I | GGNNCC | 2 cut(s) | 286, 300 |
| PspPI | GGNCC | 3 cut(s) | 4, 73, 166 |
| PsuI | RGATCY | 2 cut(s) | 79, 112 |
| RsaI | GTAC | 3 cut(s) | 19, 95, 300 |
| RsaNI | GTAC | 3 cut(s) | 18, 94, 299 |
| SalI | GTCGAC | 1 cut(s) | 276 |
| SatI | GCNGC | 3 cut(s) | 58, 61, 64 |
| Sau3AI | GATC | 2 cut(s) | 79, 112 |
| Sau96I | GGNCC | 3 cut(s) | 4, 73, 166 |
| ScrFI | CCNGG | 3 cut(s) | 36, 72, 282 |
| SetI | ASST | 7 cut(s) | 102, 139, 184, 199, 262, 343, 348 |
| SfaNI | GCATC | 1 cut(s) | 309 |
| SsiI | CCGC | 6 cut(s) | 21, 39, 64, 68, 110, 235 |
| SspMI | CTAG | 3 cut(s) | 101, 221, 267 |
| StyD4I | CCNGG | 3 cut(s) | 34, 70, 280 |
| TaiI | ACGT | 3 cut(s) | 139, 199, 262 |
| TaqI | TCGA | 1 cut(s) | 277 |
| TauI | GCSGC | 1 cut(s) | 66 |
| TscAI | CASTG | 3 cut(s) | 111, 235, 336 |
| TseFI | GTSAC | 2 cut(s) | 85, 255 |
| TseI | GCWGC | 2 cut(s) | 57, 60 |
| Tsp45I | GTSAC | 2 cut(s) | 85, 255 |
| TspDTI | ATGAA | 1 cut(s) | 107 |
| TspRI | CASTG | 3 cut(s) | 111, 235, 336 |
| XmiI | GTMKAC | 1 cut(s) | 277 |
| XspI | CTAG | 3 cut(s) | 101, 221, 267 |