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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 207 |
| AccB1I | GGYRCC | 1 cut(s) | 207 |
| AccBSI | CCGCTC | 2 cut(s) | 329, 378 |
| AccII | CGCG | 1 cut(s) | 170 |
| AclWI | GGATC | 3 cut(s) | 30, 513, 526 |
| AcsI | RAATTY | 2 cut(s) | 107, 356 |
| AfaI | GTAC | 3 cut(s) | 5, 209, 235 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 67, 85, 263, 350 |
| AjnI | CCWGG | 1 cut(s) | 513 |
| AluBI | AGCT | 1 cut(s) | 10 |
| AluI | AGCT | 1 cut(s) | 10 |
| Alw21I | GWGCWC | 1 cut(s) | 95 |
| Alw26I | GTCTC | 1 cut(s) | 173 |
| AlwI | GGATC | 3 cut(s) | 30, 513, 526 |
| AoxI | GGCC | 2 cut(s) | 341, 494 |
| ApoI | RAATTY | 2 cut(s) | 107, 356 |
| Asp718I | GGTACC | 1 cut(s) | 207 |
| AspLEI | GCGC | 2 cut(s) | 60, 455 |
| AspS9I | GGNCC | 4 cut(s) | 242, 266, 341, 501 |
| AsuC2I | CCSGG | 4 cut(s) | 339, 368, 441, 498 |
| AsuHPI | GGTGA | 2 cut(s) | 100, 352 |
| AsuNHI | GCTAGC | 1 cut(s) | 10 |
| AvaII | GGWCC | 3 cut(s) | 242, 266, 501 |
| BamHI | GGATCC | 1 cut(s) | 518 |
| BanI | GGYRCC | 1 cut(s) | 207 |
| BauI | CACGAG | 2 cut(s) | 176, 373 |
| Bbv12I | GWGCWC | 1 cut(s) | 95 |
| BccI | CCATC | 2 cut(s) | 256, 424 |
| BceAI | ACGGC | 1 cut(s) | 213 |
| BciT130I | CCWGG | 1 cut(s) | 515 |
| BcnI | CCSGG | 4 cut(s) | 339, 368, 441, 498 |
| BcoDI | GTCTC | 1 cut(s) | 173 |
| BfaI | CTAG | 3 cut(s) | 11, 17, 398 |
| BfoI | RGCGCY | 2 cut(s) | 61, 456 |
| BisI | GCNGC | 4 cut(s) | 379, 382, 464, 576 |
| BlsI | GCNGC | 4 cut(s) | 380, 383, 465, 577 |
| Bme1390I | CCNGG | 5 cut(s) | 339, 368, 441, 498, 515 |
| Bme18I | GGWCC | 3 cut(s) | 242, 266, 501 |
| BmgT120I | GGNCC | 4 cut(s) | 242, 266, 341, 501 |
| BmiI | GGNNCC | 6 cut(s) | 209, 244, 267, 342, 502, 520 |
| BmrFI | CCNGG | 5 cut(s) | 339, 368, 441, 498, 515 |
| BmsI | GCATC | 1 cut(s) | 497 |
| BmtI | GCTAGC | 1 cut(s) | 14 |
| BpmI | CTGGAG | 1 cut(s) | 497 |
| BpuMI | CCSGG | 4 cut(s) | 339, 368, 441, 498 |
| BsaJI | CCNNGG | 6 cut(s) | 338, 349, 366, 439, 497, 514 |
| BsaWI | WCCGGW | 3 cut(s) | 130, 210, 256 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 292, 322 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 67, 85, 263, 350 |
| BseBI | CCWGG | 1 cut(s) | 515 |
| BseDI | CCNNGG | 6 cut(s) | 338, 349, 366, 439, 497, 514 |
| BseGI | GGATG | 1 cut(s) | 220 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 67, 85, 263, 350 |
| Bsh1236I | CGCG | 1 cut(s) | 170 |
| Bsh1285I | CGRYCG | 1 cut(s) | 445 |
| BshFI | GGCC | 2 cut(s) | 343, 496 |
| BshNI | GGYRCC | 1 cut(s) | 207 |
| BsiEI | CGRYCG | 1 cut(s) | 445 |
| BsiHKAI | GWGCWC | 1 cut(s) | 95 |
| BsiSI | CCGG | 8 cut(s) | 131, 211, 240, 257, 338, 368, 441, 497 |
| BslFI | GGGAC | 2 cut(s) | 279, 514 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 67, 85, 263, 350 |
| BsmAI | GTCTC | 1 cut(s) | 173 |
| BsmFI | GGGAC | 2 cut(s) | 279, 514 |
| BsmI | GAATGC | 1 cut(s) | 417 |
| BsnI | GGCC | 2 cut(s) | 343, 496 |
| Bsp1286I | GDGCHC | 1 cut(s) | 95 |
| Bsp143I | GATC | 2 cut(s) | 22, 518 |
| Bsp68I | TCGCGA | 1 cut(s) | 170 |
| BspANI | GGCC | 2 cut(s) | 343, 496 |
| BspFNI | CGCG | 1 cut(s) | 170 |
| BspLI | GGNNCC | 6 cut(s) | 209, 244, 267, 342, 502, 520 |
| BspOI | GCTAGC | 1 cut(s) | 14 |
| BspPI | GGATC | 3 cut(s) | 30, 513, 526 |
| BspT107I | GGYRCC | 1 cut(s) | 207 |
| BsrBI | CCGCTC | 2 cut(s) | 329, 378 |
| BssECI | CCNNGG | 6 cut(s) | 338, 349, 366, 439, 497, 514 |
| BssMI | GATC | 2 cut(s) | 22, 518 |
| BssSI | CACGAG | 2 cut(s) | 176, 373 |
| Bst2BI | CACGAG | 2 cut(s) | 176, 373 |
| Bst2UI | CCWGG | 1 cut(s) | 515 |
| Bst4CI | ACNGT | 1 cut(s) | 505 |
| BstC8I | GCNNGC | 5 cut(s) | 12, 95, 162, 528, 539 |
| BstDEI | CTNAG | 1 cut(s) | 403 |
| BstF5I | GGATG | 1 cut(s) | 220 |
| BstFNI | CGCG | 1 cut(s) | 170 |
| BstH2I | RGCGCY | 2 cut(s) | 61, 456 |
| BstHHI | GCGC | 2 cut(s) | 60, 455 |
| BstKTI | GATC | 2 cut(s) | 25, 521 |
| BstMAI | GTCTC | 1 cut(s) | 173 |
| BstMBI | GATC | 2 cut(s) | 22, 518 |
| BstMCI | CGRYCG | 1 cut(s) | 445 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 134, 236 |
| BstNI | CCWGG | 1 cut(s) | 515 |
| BstNSI | RCATGY | 1 cut(s) | 230 |
| BstSCI | CCNGG | 5 cut(s) | 337, 366, 439, 496, 513 |
| BstUI | CGCG | 1 cut(s) | 170 |
| BstX2I | RGATCY | 2 cut(s) | 22, 518 |
| BstYI | RGATCY | 2 cut(s) | 22, 518 |
| BsuRI | GGCC | 2 cut(s) | 343, 496 |
| BtsCI | GGATG | 1 cut(s) | 220 |
| BtuMI | TCGCGA | 1 cut(s) | 170 |
| Cac8I | GCNNGC | 5 cut(s) | 12, 95, 162, 528, 539 |
| CfoI | GCGC | 2 cut(s) | 60, 455 |
| Cfr13I | GGNCC | 4 cut(s) | 242, 266, 341, 501 |
| Csp6I | GTAC | 3 cut(s) | 4, 208, 234 |
| CviAII | CATG | 2 cut(s) | 227, 579 |
| CviJI | RGCY | 8 cut(s) | 10, 77, 118, 187, 337, 343, 434, 496 |
| CviKI_1 | RGCY | 8 cut(s) | 10, 77, 118, 187, 337, 343, 434, 496 |
| CviQI | GTAC | 3 cut(s) | 4, 208, 234 |
| DdeI | CTNAG | 1 cut(s) | 403 |
| DpnI | GATC | 2 cut(s) | 24, 520 |
| DpnII | GATC | 2 cut(s) | 22, 518 |
| EciI | GGCGGA | 4 cut(s) | 215, 319, 399, 524 |
| Eco47I | GGWCC | 3 cut(s) | 242, 266, 501 |
| EcoRII | CCWGG | 1 cut(s) | 513 |
| FaeI | CATG | 2 cut(s) | 230, 582 |
| FaiI | YATR | 4 cut(s) | 228, 543, 545, 580 |
| FaqI | GGGAC | 2 cut(s) | 279, 514 |
| FatI | CATG | 2 cut(s) | 226, 578 |
| FauI | CCCGC | 2 cut(s) | 468, 546 |
| FauNDI | CATATG | 1 cut(s) | 543 |
| Fnu4HI | GCNGC | 4 cut(s) | 379, 382, 464, 576 |
| FokI | GGATG | 1 cut(s) | 207 |
| Fsp4HI | GCNGC | 4 cut(s) | 379, 382, 464, 576 |
| FspBI | CTAG | 3 cut(s) | 11, 17, 398 |
| GlaI | GCGC | 2 cut(s) | 59, 454 |
| GluI | GCNGC | 4 cut(s) | 379, 382, 464, 576 |
| GsuI | CTGGAG | 1 cut(s) | 497 |
| HaeII | RGCGCY | 2 cut(s) | 61, 456 |
| HaeIII | GGCC | 2 cut(s) | 343, 496 |
| HapII | CCGG | 8 cut(s) | 131, 211, 240, 257, 338, 368, 441, 497 |
| HhaI | GCGC | 2 cut(s) | 60, 455 |
| Hin1II | CATG | 2 cut(s) | 230, 582 |
| Hin6I | GCGC | 2 cut(s) | 58, 453 |
| HinP1I | GCGC | 2 cut(s) | 58, 453 |
| HinfI | GANTC | 2 cut(s) | 307, 387 |
| HpaII | CCGG | 8 cut(s) | 131, 211, 240, 257, 338, 368, 441, 497 |
| HphI | GGTGA | 2 cut(s) | 100, 352 |
| Hpy166II | GTNNAC | 1 cut(s) | 508 |
| Hpy188I | TCNGA | 1 cut(s) | 425 |
| Hpy188III | TCNNGA | 1 cut(s) | 169 |
| Hpy8I | GTNNAC | 1 cut(s) | 508 |
| HpyAV | CCTTC | 1 cut(s) | 61 |
| HpyCH4III | ACNGT | 1 cut(s) | 505 |
| HpyCH4V | TGCA | 3 cut(s) | 230, 415, 547 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 134, 236 |
| HpyF3I | CTNAG | 1 cut(s) | 403 |
| Hsp92II | CATG | 2 cut(s) | 230, 582 |
| HspAI | GCGC | 2 cut(s) | 58, 453 |
| KpnI | GGTACC | 1 cut(s) | 211 |
| Kzo9I | GATC | 2 cut(s) | 22, 518 |
| LmnI | GCTCC | 3 cut(s) | 43, 55, 294 |
| LweI | GCATC | 1 cut(s) | 497 |
| MaeI | CTAG | 3 cut(s) | 11, 17, 398 |
| MaeIII | GTNAC | 2 cut(s) | 308, 563 |
| MalI | GATC | 2 cut(s) | 24, 520 |
| MbiI | CCGCTC | 2 cut(s) | 329, 378 |
| MboI | GATC | 2 cut(s) | 22, 518 |
| MflI | RGATCY | 2 cut(s) | 22, 518 |
| MhlI | GDGCHC | 1 cut(s) | 95 |
| MluCI | AATT | 3 cut(s) | 107, 251, 356 |
| MlyI | GAGTC | 1 cut(s) | 316 |
| MnlI | CCTC | 9 cut(s) | 64, 127, 285, 294, 344, 373, 489, 521, 532 |
| MslI | CAYNNNNRTG | 2 cut(s) | 98, 225 |
| MspI | CCGG | 8 cut(s) | 131, 211, 240, 257, 338, 368, 441, 497 |
| MspR9I | CCNGG | 5 cut(s) | 339, 368, 441, 498, 515 |
| Mva1269I | GAATGC | 1 cut(s) | 417 |
| MvaI | CCWGG | 1 cut(s) | 515 |
| MvnI | CGCG | 1 cut(s) | 170 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 134, 236 |
| NciI | CCSGG | 4 cut(s) | 339, 368, 441, 498 |
| NdeI | CATATG | 1 cut(s) | 543 |
| NdeII | GATC | 2 cut(s) | 22, 518 |
| NheI | GCTAGC | 1 cut(s) | 10 |
| NlaIII | CATG | 2 cut(s) | 230, 582 |
| NlaIV | GGNNCC | 6 cut(s) | 209, 244, 267, 342, 502, 520 |
| NmeAIII | GCCGAG | 1 cut(s) | 86 |
| NmuCI | GTSAC | 2 cut(s) | 308, 563 |
| NruI | TCGCGA | 1 cut(s) | 170 |
| NspI | RCATGY | 1 cut(s) | 230 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 174 |
| PctI | GAATGC | 1 cut(s) | 417 |
| PfeI | GAWTC | 1 cut(s) | 387 |
| PkrI | GCNGC | 4 cut(s) | 380, 383, 465, 577 |
| PleI | GAGTC | 1 cut(s) | 315 |
| PpsI | GAGTC | 1 cut(s) | 315 |
| Psp6I | CCWGG | 1 cut(s) | 513 |
| PspGI | CCWGG | 1 cut(s) | 513 |
| PspN4I | GGNNCC | 6 cut(s) | 209, 244, 267, 342, 502, 520 |
| PspPI | GGNCC | 4 cut(s) | 242, 266, 341, 501 |
| PsuI | RGATCY | 2 cut(s) | 22, 518 |
| RruI | TCGCGA | 1 cut(s) | 170 |
| RsaI | GTAC | 3 cut(s) | 5, 209, 235 |
| RsaNI | GTAC | 3 cut(s) | 4, 208, 234 |
| RseI | CAYNNNNRTG | 2 cut(s) | 98, 225 |
| SatI | GCNGC | 4 cut(s) | 379, 382, 464, 576 |
| Sau3AI | GATC | 2 cut(s) | 22, 518 |
| Sau96I | GGNCC | 4 cut(s) | 242, 266, 341, 501 |
| SchI | GAGTC | 1 cut(s) | 316 |
| ScrFI | CCNGG | 5 cut(s) | 339, 368, 441, 498, 515 |
| SduI | GDGCHC | 1 cut(s) | 95 |
| SetI | ASST | 4 cut(s) | 12, 90, 365, 513 |
| SfaNI | GCATC | 1 cut(s) | 497 |
| SinI | GGWCC | 3 cut(s) | 242, 266, 501 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 98, 225 |
| Sse9I | AATT | 3 cut(s) | 107, 251, 356 |
| SspMI | CTAG | 3 cut(s) | 11, 17, 398 |
| StyD4I | CCNGG | 5 cut(s) | 337, 366, 439, 496, 513 |
| TaaI | ACNGT | 1 cut(s) | 505 |
| TaqI | TCGA | 1 cut(s) | 445 |
| TasI | AATT | 3 cut(s) | 107, 251, 356 |
| TauI | GCSGC | 4 cut(s) | 381, 384, 466, 578 |
| TfiI | GAWTC | 1 cut(s) | 387 |
| TseFI | GTSAC | 2 cut(s) | 308, 563 |
| Tsp45I | GTSAC | 2 cut(s) | 308, 563 |
| TspGWI | ACGGA | 1 cut(s) | 307 |
| VpaK11BI | GGWCC | 3 cut(s) | 242, 266, 501 |
| XapI | RAATTY | 2 cut(s) | 107, 356 |
| XceI | RCATGY | 1 cut(s) | 230 |
| XspI | CTAG | 3 cut(s) | 11, 17, 398 |