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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 365 |
| AccB1I | GGYRCC | 1 cut(s) | 365 |
| AccII | CGCG | 2 cut(s) | 90, 92 |
| AciI | CCGC | 7 cut(s) | 15, 74, 88, 149, 191, 312, 400 |
| AclWI | GGATC | 1 cut(s) | 201 |
| AcoI | YGGCCR | 2 cut(s) | 48, 261 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 1 cut(s) | 367 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 77, 368 |
| AjnI | CCWGG | 3 cut(s) | 151, 347, 361 |
| AleI | CACNNNNGTG | 1 cut(s) | 269 |
| AluBI | AGCT | 1 cut(s) | 289 |
| AluI | AGCT | 1 cut(s) | 289 |
| Alw21I | GWGCWC | 1 cut(s) | 347 |
| Alw26I | GTCTC | 1 cut(s) | 119 |
| AlwI | GGATC | 1 cut(s) | 201 |
| AoxI | GGCC | 4 cut(s) | 48, 154, 246, 261 |
| Asp718I | GGTACC | 1 cut(s) | 365 |
| AspLEI | GCGC | 2 cut(s) | 92, 374 |
| AspS9I | GGNCC | 2 cut(s) | 247, 397 |
| AsuC2I | CCSGG | 2 cut(s) | 107, 117 |
| AsuHPI | GGTGA | 1 cut(s) | 91 |
| AvaII | GGWCC | 1 cut(s) | 397 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| BanI | GGYRCC | 1 cut(s) | 365 |
| Bbv12I | GWGCWC | 1 cut(s) | 347 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 27, 61 |
| BciT130I | CCWGG | 3 cut(s) | 153, 349, 363 |
| BcnI | CCSGG | 2 cut(s) | 107, 117 |
| BcoDI | GTCTC | 1 cut(s) | 119 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| Bme1390I | CCNGG | 5 cut(s) | 107, 117, 153, 349, 363 |
| Bme18I | GGWCC | 1 cut(s) | 397 |
| BmgT120I | GGNCC | 2 cut(s) | 247, 397 |
| BmiI | GGNNCC | 1 cut(s) | 367 |
| BmrFI | CCNGG | 5 cut(s) | 107, 117, 153, 349, 363 |
| BmsI | GCATC | 1 cut(s) | 90 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| BpuMI | CCSGG | 2 cut(s) | 107, 117 |
| BsaJI | CCNNGG | 3 cut(s) | 105, 115, 243 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 77, 368 |
| Bse118I | RCCGGY | 1 cut(s) | 368 |
| Bse1I | ACTGG | 2 cut(s) | 43, 192 |
| BseBI | CCWGG | 3 cut(s) | 153, 349, 363 |
| BseDI | CCNNGG | 3 cut(s) | 105, 115, 243 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 77, 368 |
| BseNI | ACTGG | 2 cut(s) | 43, 192 |
| BseYI | CCCAGC | 1 cut(s) | 70 |
| Bsh1236I | CGCG | 2 cut(s) | 90, 92 |
| BshFI | GGCC | 4 cut(s) | 50, 156, 248, 263 |
| BshNI | GGYRCC | 1 cut(s) | 365 |
| BsiHKAI | GWGCWC | 1 cut(s) | 347 |
| BsiSI | CCGG | 3 cut(s) | 107, 117, 369 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 77, 368 |
| BsmAI | GTCTC | 1 cut(s) | 119 |
| BsnI | GGCC | 4 cut(s) | 50, 156, 248, 263 |
| Bsp1286I | GDGCHC | 1 cut(s) | 347 |
| Bsp143I | GATC | 1 cut(s) | 193 |
| BspACI | CCGC | 7 cut(s) | 15, 74, 88, 149, 191, 312, 400 |
| BspANI | GGCC | 4 cut(s) | 50, 156, 248, 263 |
| BspFNI | CGCG | 2 cut(s) | 90, 92 |
| BspLI | GGNNCC | 1 cut(s) | 367 |
| BspPI | GGATC | 1 cut(s) | 201 |
| BspT107I | GGYRCC | 1 cut(s) | 365 |
| BsrFI | RCCGGY | 1 cut(s) | 368 |
| BsrI | ACTGG | 2 cut(s) | 43, 192 |
| BssAI | RCCGGY | 1 cut(s) | 368 |
| BssECI | CCNNGG | 3 cut(s) | 105, 115, 243 |
| BssMI | GATC | 1 cut(s) | 193 |
| Bst2UI | CCWGG | 3 cut(s) | 153, 349, 363 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstC8I | GCNNGC | 2 cut(s) | 149, 332 |
| BstFNI | CGCG | 2 cut(s) | 90, 92 |
| BstHHI | GCGC | 2 cut(s) | 92, 374 |
| BstKTI | GATC | 1 cut(s) | 196 |
| BstMAI | GTCTC | 1 cut(s) | 119 |
| BstMBI | GATC | 1 cut(s) | 193 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 87, 153 |
| BstNI | CCWGG | 3 cut(s) | 153, 349, 363 |
| BstSCI | CCNGG | 5 cut(s) | 105, 115, 151, 347, 361 |
| BstUI | CGCG | 2 cut(s) | 90, 92 |
| BstX2I | RGATCY | 1 cut(s) | 193 |
| BstYI | RGATCY | 1 cut(s) | 193 |
| BsuRI | GGCC | 4 cut(s) | 50, 156, 248, 263 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| Cac8I | GCNNGC | 2 cut(s) | 149, 332 |
| CfoI | GCGC | 2 cut(s) | 92, 374 |
| Cfr10I | RCCGGY | 1 cut(s) | 368 |
| Cfr13I | GGNCC | 2 cut(s) | 247, 397 |
| CsiI | ACCWGGT | 1 cut(s) | 361 |
| Csp6I | GTAC | 1 cut(s) | 366 |
| CviJI | RGCY | 7 cut(s) | 50, 120, 156, 248, 263, 289, 357 |
| CviKI_1 | RGCY | 7 cut(s) | 50, 120, 156, 248, 263, 289, 357 |
| CviQI | GTAC | 1 cut(s) | 366 |
| DpnI | GATC | 1 cut(s) | 195 |
| DpnII | GATC | 1 cut(s) | 193 |
| EaeI | YGGCCR | 2 cut(s) | 48, 261 |
| EciI | GGCGGA | 1 cut(s) | 206 |
| Eco47I | GGWCC | 1 cut(s) | 397 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoRII | CCWGG | 3 cut(s) | 151, 347, 361 |
| FaiI | YATR | 3 cut(s) | 175, 177, 354 |
| FauI | CCCGC | 2 cut(s) | 8, 156 |
| GlaI | GCGC | 2 cut(s) | 91, 373 |
| GsaI | CCCAGC | 1 cut(s) | 74 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HaeIII | GGCC | 4 cut(s) | 50, 156, 248, 263 |
| HapII | CCGG | 3 cut(s) | 107, 117, 369 |
| HhaI | GCGC | 2 cut(s) | 92, 374 |
| Hin6I | GCGC | 2 cut(s) | 90, 372 |
| HinP1I | GCGC | 2 cut(s) | 90, 372 |
| HpaII | CCGG | 3 cut(s) | 107, 117, 369 |
| HphI | GGTGA | 1 cut(s) | 91 |
| Hpy166II | GTNNAC | 2 cut(s) | 112, 221 |
| Hpy8I | GTNNAC | 2 cut(s) | 112, 221 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 87, 153 |
| HspAI | GCGC | 2 cut(s) | 90, 372 |
| KpnI | GGTACC | 1 cut(s) | 369 |
| Kzo9I | GATC | 1 cut(s) | 193 |
| LmnI | GCTCC | 1 cut(s) | 342 |
| LweI | GCATC | 1 cut(s) | 90 |
| MabI | ACCWGGT | 1 cut(s) | 361 |
| MaeIII | GTNAC | 2 cut(s) | 273, 336 |
| MalI | GATC | 1 cut(s) | 195 |
| MboI | GATC | 1 cut(s) | 193 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MflI | RGATCY | 1 cut(s) | 193 |
| MhlI | GDGCHC | 1 cut(s) | 347 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluCI | AATT | 1 cut(s) | 392 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MnlI | CCTC | 4 cut(s) | 173, 178, 238, 298 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MslI | CAYNNNNRTG | 1 cut(s) | 269 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 1 cut(s) | 74 |
| MspI | CCGG | 3 cut(s) | 107, 117, 369 |
| MspR9I | CCNGG | 5 cut(s) | 107, 117, 153, 349, 363 |
| MvaI | CCWGG | 3 cut(s) | 153, 349, 363 |
| MvnI | CGCG | 2 cut(s) | 90, 92 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 87, 153 |
| NciI | CCSGG | 2 cut(s) | 107, 117 |
| NdeII | GATC | 1 cut(s) | 193 |
| NlaIV | GGNNCC | 1 cut(s) | 367 |
| NmuCI | GTSAC | 2 cut(s) | 273, 336 |
| OliI | CACNNNNGTG | 1 cut(s) | 269 |
| Psp6I | CCWGG | 3 cut(s) | 151, 347, 361 |
| PspFI | CCCAGC | 1 cut(s) | 70 |
| PspGI | CCWGG | 3 cut(s) | 151, 347, 361 |
| PspN4I | GGNNCC | 1 cut(s) | 367 |
| PspPI | GGNCC | 2 cut(s) | 247, 397 |
| PsuI | RGATCY | 1 cut(s) | 193 |
| RsaI | GTAC | 1 cut(s) | 367 |
| RsaNI | GTAC | 1 cut(s) | 366 |
| RseI | CAYNNNNRTG | 1 cut(s) | 269 |
| Sau3AI | GATC | 1 cut(s) | 193 |
| Sau96I | GGNCC | 2 cut(s) | 247, 397 |
| ScrFI | CCNGG | 5 cut(s) | 107, 117, 153, 349, 363 |
| SduI | GDGCHC | 1 cut(s) | 347 |
| SetI | ASST | 3 cut(s) | 165, 291, 364 |
| SexAI | ACCWGGT | 1 cut(s) | 361 |
| SfaNI | GCATC | 1 cut(s) | 90 |
| SinI | GGWCC | 1 cut(s) | 397 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 269 |
| Sse9I | AATT | 1 cut(s) | 392 |
| SsiI | CCGC | 7 cut(s) | 15, 74, 88, 149, 191, 312, 400 |
| StyD4I | CCNGG | 5 cut(s) | 105, 115, 151, 347, 361 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaqII | GACCGA | 1 cut(s) | 257 |
| TasI | AATT | 1 cut(s) | 392 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 2 cut(s) | 273, 336 |
| Tsp45I | GTSAC | 2 cut(s) | 273, 336 |
| TspGWI | ACGGA | 1 cut(s) | 355 |
| TspRI | CASTG | 1 cut(s) | 192 |
| VpaK11BI | GGWCC | 1 cut(s) | 397 |