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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 145 |
| Acc36I | ACCTGC | 1 cut(s) | 145 |
| AccII | CGCG | 1 cut(s) | 117 |
| AciI | CCGC | 2 cut(s) | 48, 89 |
| AcoI | YGGCCR | 1 cut(s) | 159 |
| AluBI | AGCT | 2 cut(s) | 79, 187 |
| AluI | AGCT | 2 cut(s) | 79, 187 |
| AoxI | GGCC | 3 cut(s) | 36, 138, 159 |
| AspLEI | GCGC | 3 cut(s) | 117, 129, 199 |
| AspS9I | GGNCC | 1 cut(s) | 91 |
| AsuHPI | GGTGA | 1 cut(s) | 178 |
| AsuNHI | GCTAGC | 1 cut(s) | 79 |
| AvaII | GGWCC | 1 cut(s) | 91 |
| BalI | TGGCCA | 1 cut(s) | 161 |
| BccI | CCATC | 1 cut(s) | 65 |
| BfaI | CTAG | 2 cut(s) | 80, 86 |
| BfoI | RGCGCY | 1 cut(s) | 200 |
| BfuAI | ACCTGC | 1 cut(s) | 145 |
| Bme18I | GGWCC | 1 cut(s) | 91 |
| BmgT120I | GGNCC | 1 cut(s) | 91 |
| BmrI | ACTGGG | 1 cut(s) | 64 |
| BmtI | GCTAGC | 1 cut(s) | 83 |
| BmuI | ACTGGG | 1 cut(s) | 64 |
| BsaJI | CCNNGG | 2 cut(s) | 141, 162 |
| Bse1I | ACTGG | 1 cut(s) | 70 |
| BseDI | CCNNGG | 2 cut(s) | 141, 162 |
| BseGI | GGATG | 1 cut(s) | 57 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseNI | ACTGG | 1 cut(s) | 70 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseYI | CCCAGC | 1 cut(s) | 217 |
| Bsh1236I | CGCG | 1 cut(s) | 117 |
| BshFI | GGCC | 3 cut(s) | 38, 140, 161 |
| BsnI | GGCC | 3 cut(s) | 38, 140, 161 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| Bsp19I | CCATGG | 1 cut(s) | 162 |
| BspACI | CCGC | 2 cut(s) | 48, 89 |
| BspANI | GGCC | 3 cut(s) | 38, 140, 161 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspFNI | CGCG | 1 cut(s) | 117 |
| BspMI | ACCTGC | 1 cut(s) | 145 |
| BspOI | GCTAGC | 1 cut(s) | 83 |
| BsrI | ACTGG | 1 cut(s) | 70 |
| BssECI | CCNNGG | 2 cut(s) | 141, 162 |
| BssMI | GATC | 1 cut(s) | 40 |
| BssT1I | CCWWGG | 1 cut(s) | 162 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 81, 119 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstDSI | CCRYGG | 1 cut(s) | 162 |
| BstF5I | GGATG | 1 cut(s) | 57 |
| BstFNI | CGCG | 1 cut(s) | 117 |
| BstH2I | RGCGCY | 1 cut(s) | 200 |
| BstHHI | GCGC | 3 cut(s) | 117, 129, 199 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 203, 218 |
| BstUI | CGCG | 1 cut(s) | 117 |
| BsuRI | GGCC | 3 cut(s) | 38, 140, 161 |
| BtgI | CCRYGG | 1 cut(s) | 162 |
| BtsCI | GGATG | 1 cut(s) | 57 |
| BtsIMutI | CAGTG | 1 cut(s) | 24 |
| BveI | ACCTGC | 1 cut(s) | 145 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 81, 119 |
| CfoI | GCGC | 3 cut(s) | 117, 129, 199 |
| Cfr13I | GGNCC | 1 cut(s) | 91 |
| CviAII | CATG | 1 cut(s) | 163 |
| CviJI | RGCY | 5 cut(s) | 38, 79, 140, 161, 187 |
| CviKI_1 | RGCY | 5 cut(s) | 38, 79, 140, 161, 187 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| EaeI | YGGCCR | 1 cut(s) | 159 |
| EciI | GGCGGA | 1 cut(s) | 37 |
| Eco130I | CCWWGG | 1 cut(s) | 162 |
| Eco47I | GGWCC | 1 cut(s) | 91 |
| EcoT14I | CCWWGG | 1 cut(s) | 162 |
| ErhI | CCWWGG | 1 cut(s) | 162 |
| FaeI | CATG | 1 cut(s) | 166 |
| FaiI | YATR | 2 cut(s) | 75, 164 |
| FatI | CATG | 1 cut(s) | 162 |
| FokI | GGATG | 1 cut(s) | 44 |
| FspBI | CTAG | 2 cut(s) | 80, 86 |
| GlaI | GCGC | 3 cut(s) | 116, 128, 198 |
| GsaI | CCCAGC | 1 cut(s) | 221 |
| HaeII | RGCGCY | 1 cut(s) | 200 |
| HaeIII | GGCC | 3 cut(s) | 38, 140, 161 |
| HhaI | GCGC | 3 cut(s) | 117, 129, 199 |
| Hin1II | CATG | 1 cut(s) | 166 |
| Hin6I | GCGC | 3 cut(s) | 115, 127, 197 |
| HinP1I | GCGC | 3 cut(s) | 115, 127, 197 |
| HphI | GGTGA | 1 cut(s) | 178 |
| Hpy188III | TCNNGA | 2 cut(s) | 110, 174 |
| HpyAV | CCTTC | 1 cut(s) | 104 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 203, 218 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| Hsp92II | CATG | 1 cut(s) | 166 |
| HspAI | GCGC | 3 cut(s) | 115, 127, 197 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LmnI | GCTCC | 2 cut(s) | 31, 124 |
| LpnPI | CCDG | 5 cut(s) | 20, 83, 107, 140, 185 |
| MaeI | CTAG | 2 cut(s) | 80, 86 |
| MalI | GATC | 1 cut(s) | 42 |
| MboI | GATC | 1 cut(s) | 40 |
| MlsI | TGGCCA | 1 cut(s) | 161 |
| MluNI | TGGCCA | 1 cut(s) | 161 |
| MnlI | CCTC | 2 cut(s) | 22, 136 |
| Mox20I | TGGCCA | 1 cut(s) | 161 |
| MscI | TGGCCA | 1 cut(s) | 161 |
| MslI | CAYNNNNRTG | 1 cut(s) | 167 |
| Msp20I | TGGCCA | 1 cut(s) | 161 |
| MvnI | CGCG | 1 cut(s) | 117 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 203, 218 |
| NcoI | CCATGG | 1 cut(s) | 162 |
| NdeII | GATC | 1 cut(s) | 40 |
| NheI | GCTAGC | 1 cut(s) | 79 |
| NlaIII | CATG | 1 cut(s) | 166 |
| NmeAIII | GCCGAG | 1 cut(s) | 166 |
| PaqCI | CACCTGC | 1 cut(s) | 145 |
| PspFI | CCCAGC | 1 cut(s) | 217 |
| PspPI | GGNCC | 1 cut(s) | 91 |
| RseI | CAYNNNNRTG | 1 cut(s) | 167 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 1 cut(s) | 91 |
| SetI | ASST | 4 cut(s) | 81, 96, 159, 189 |
| SinI | GGWCC | 1 cut(s) | 91 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 167 |
| SsiI | CCGC | 2 cut(s) | 48, 89 |
| SspMI | CTAG | 2 cut(s) | 80, 86 |
| StyI | CCWWGG | 1 cut(s) | 162 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaqI | TCGA | 1 cut(s) | 109 |
| TscAI | CASTG | 1 cut(s) | 31 |
| TspRI | CASTG | 1 cut(s) | 31 |
| VpaK11BI | GGWCC | 1 cut(s) | 91 |
| XspI | CTAG | 2 cut(s) | 80, 86 |