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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 247 |
| Acc36I | ACCTGC | 2 cut(s) | 247, 394 |
| AccB1I | GGYRCC | 1 cut(s) | 659 |
| AccBSI | CCGCTC | 2 cut(s) | 602, 650 |
| AccII | CGCG | 1 cut(s) | 90 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AclWI | GGATC | 1 cut(s) | 562 |
| AcoI | YGGCCR | 2 cut(s) | 48, 261 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 2 cut(s) | 367, 453 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 73, 256, 368 |
| AgsI | TTSAA | 1 cut(s) | 678 |
| AjnI | CCWGG | 5 cut(s) | 115, 151, 347, 700, 705 |
| AleI | CACNNNNGTG | 2 cut(s) | 107, 269 |
| AluBI | AGCT | 2 cut(s) | 181, 427 |
| AluI | AGCT | 2 cut(s) | 181, 427 |
| Alw26I | GTCTC | 2 cut(s) | 119, 502 |
| AlwI | GGATC | 1 cut(s) | 562 |
| AoxI | GGCC | 8 cut(s) | 48, 154, 240, 246, 261, 440, 575, 703 |
| ApeKI | GCWGC | 2 cut(s) | 141, 622 |
| AspLEI | GCGC | 4 cut(s) | 92, 231, 374, 670 |
| AspS9I | GGNCC | 6 cut(s) | 247, 406, 415, 440, 576, 709 |
| AsuC2I | CCSGG | 3 cut(s) | 71, 320, 574 |
| AsuHPI | GGTGA | 2 cut(s) | 157, 708 |
| AvaII | GGWCC | 3 cut(s) | 406, 415, 709 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| BanI | GGYRCC | 1 cut(s) | 659 |
| BbvI | GCAGC | 2 cut(s) | 153, 634 |
| BccI | CCATC | 2 cut(s) | 59, 507 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 27, 61, 482, 516 |
| BciT130I | CCWGG | 5 cut(s) | 117, 153, 349, 702, 707 |
| BcnI | CCSGG | 3 cut(s) | 71, 320, 574 |
| BcoDI | GTCTC | 2 cut(s) | 119, 502 |
| BfaI | CTAG | 1 cut(s) | 363 |
| BfoI | RGCGCY | 1 cut(s) | 671 |
| BfuAI | ACCTGC | 2 cut(s) | 247, 394 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 153, 320, 588 |
| BisI | GCNGC | 4 cut(s) | 142, 145, 443, 623 |
| BlsI | GCNGC | 4 cut(s) | 143, 146, 444, 624 |
| Bme1390I | CCNGG | 8 cut(s) | 71, 117, 153, 320, 349, 574, 702, 707 |
| Bme18I | GGWCC | 3 cut(s) | 406, 415, 709 |
| BmgT120I | GGNCC | 6 cut(s) | 247, 406, 415, 440, 576, 709 |
| BmiI | GGNNCC | 4 cut(s) | 408, 417, 441, 661 |
| BmrFI | CCNGG | 8 cut(s) | 71, 117, 153, 320, 349, 574, 702, 707 |
| BmrI | ACTGGG | 2 cut(s) | 101, 451 |
| BmsI | GCATC | 2 cut(s) | 583, 609 |
| BmuI | ACTGGG | 2 cut(s) | 101, 451 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| BpuEI | CTTGAG | 1 cut(s) | 526 |
| BpuMI | CCSGG | 3 cut(s) | 71, 320, 574 |
| BsaI | GGTCTC | 1 cut(s) | 502 |
| BsaJI | CCNNGG | 4 cut(s) | 115, 243, 512, 525 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 623, 653 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 73, 256, 368 |
| Bse118I | RCCGGY | 1 cut(s) | 368 |
| Bse1I | ACTGG | 6 cut(s) | 43, 107, 192, 389, 457, 615 |
| BseBI | CCWGG | 5 cut(s) | 117, 153, 349, 702, 707 |
| BseDI | CCNNGG | 4 cut(s) | 115, 243, 512, 525 |
| BseGI | GGATG | 2 cut(s) | 51, 624 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 73, 256, 368 |
| BseMII | CTCAG | 2 cut(s) | 281, 317 |
| BseNI | ACTGG | 6 cut(s) | 43, 107, 192, 389, 457, 615 |
| BseXI | GCAGC | 2 cut(s) | 153, 634 |
| BseYI | CCCAGC | 1 cut(s) | 664 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| BshFI | GGCC | 8 cut(s) | 50, 156, 242, 248, 263, 442, 577, 705 |
| BshNI | GGYRCC | 1 cut(s) | 659 |
| BsiSI | CCGG | 4 cut(s) | 71, 320, 369, 574 |
| BslFI | GGGAC | 1 cut(s) | 392 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 73, 256, 368 |
| BsmAI | GTCTC | 2 cut(s) | 119, 502 |
| BsmFI | GGGAC | 1 cut(s) | 392 |
| BsmI | GAATGC | 1 cut(s) | 574 |
| BsnI | GGCC | 8 cut(s) | 50, 156, 242, 248, 263, 442, 577, 705 |
| Bso31I | GGTCTC | 1 cut(s) | 502 |
| Bsp143I | GATC | 2 cut(s) | 193, 554 |
| BspANI | GGCC | 8 cut(s) | 50, 156, 242, 248, 263, 442, 577, 705 |
| BspCNI | CTCAG | 2 cut(s) | 282, 318 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspLI | GGNNCC | 4 cut(s) | 408, 417, 441, 661 |
| BspMI | ACCTGC | 2 cut(s) | 247, 394 |
| BspPI | GGATC | 1 cut(s) | 562 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 659 |
| BspTNI | GGTCTC | 1 cut(s) | 502 |
| BsrBI | CCGCTC | 2 cut(s) | 602, 650 |
| BsrFI | RCCGGY | 1 cut(s) | 368 |
| BsrI | ACTGG | 6 cut(s) | 43, 107, 192, 389, 457, 615 |
| BssAI | RCCGGY | 1 cut(s) | 368 |
| BssECI | CCNNGG | 4 cut(s) | 115, 243, 512, 525 |
| BssMI | GATC | 2 cut(s) | 193, 554 |
| BssT1I | CCWWGG | 1 cut(s) | 512 |
| Bst2UI | CCWGG | 5 cut(s) | 117, 153, 349, 702, 707 |
| Bst4CI | ACNGT | 1 cut(s) | 336 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 183, 589 |
| BstDEI | CTNAG | 2 cut(s) | 290, 326 |
| BstF5I | GGATG | 2 cut(s) | 51, 624 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstH2I | RGCGCY | 1 cut(s) | 671 |
| BstHHI | GCGC | 4 cut(s) | 92, 231, 374, 670 |
| BstKTI | GATC | 2 cut(s) | 196, 557 |
| BstMAI | GTCTC | 2 cut(s) | 119, 502 |
| BstMBI | GATC | 2 cut(s) | 193, 554 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 153, 320, 409, 588, 593, 631 |
| BstNI | CCWGG | 5 cut(s) | 117, 153, 349, 702, 707 |
| BstSCI | CCNGG | 8 cut(s) | 69, 115, 151, 318, 347, 572, 700, 705 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 2 cut(s) | 153, 634 |
| BstX2I | RGATCY | 1 cut(s) | 554 |
| BstYI | RGATCY | 1 cut(s) | 554 |
| BsuRI | GGCC | 8 cut(s) | 50, 156, 242, 248, 263, 442, 577, 705 |
| BtgZI | GCGATG | 2 cut(s) | 485, 606 |
| BtsCI | GGATG | 2 cut(s) | 51, 624 |
| BtsI | GCAGTG | 1 cut(s) | 488 |
| BtsIMutI | CAGTG | 4 cut(s) | 114, 185, 323, 488 |
| BveI | ACCTGC | 2 cut(s) | 247, 394 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 183, 589 |
| CfoI | GCGC | 4 cut(s) | 92, 231, 374, 670 |
| Cfr10I | RCCGGY | 1 cut(s) | 368 |
| Cfr13I | GGNCC | 6 cut(s) | 247, 406, 415, 440, 576, 709 |
| Csp6I | GTAC | 2 cut(s) | 366, 452 |
| CviQI | GTAC | 2 cut(s) | 366, 452 |
| DdeI | CTNAG | 2 cut(s) | 290, 326 |
| DpnI | GATC | 2 cut(s) | 195, 556 |
| DpnII | GATC | 2 cut(s) | 193, 554 |
| EaeI | YGGCCR | 2 cut(s) | 48, 261 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 1 cut(s) | 135 |
| Eco130I | CCWWGG | 1 cut(s) | 512 |
| Eco31I | GGTCTC | 1 cut(s) | 502 |
| Eco47I | GGWCC | 3 cut(s) | 406, 415, 709 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoO109I | RGGNCCY | 1 cut(s) | 406 |
| EcoRII | CCWGG | 5 cut(s) | 115, 151, 347, 700, 705 |
| EcoT14I | CCWWGG | 1 cut(s) | 512 |
| ErhI | CCWWGG | 1 cut(s) | 512 |
| FaiI | YATR | 4 cut(s) | 177, 201, 399, 401 |
| FaqI | GGGAC | 1 cut(s) | 392 |
| FauI | CCCGC | 4 cut(s) | 8, 74, 417, 592 |
| FauNDI | CATATG | 1 cut(s) | 399 |
| Fnu4HI | GCNGC | 4 cut(s) | 142, 145, 443, 623 |
| FokI | GGATG | 2 cut(s) | 38, 631 |
| Fsp4HI | GCNGC | 4 cut(s) | 142, 145, 443, 623 |
| FspBI | CTAG | 1 cut(s) | 363 |
| GlaI | GCGC | 4 cut(s) | 91, 230, 373, 669 |
| GluI | GCNGC | 4 cut(s) | 142, 145, 443, 623 |
| GsaI | CCCAGC | 1 cut(s) | 668 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HaeII | RGCGCY | 1 cut(s) | 671 |
| HaeIII | GGCC | 8 cut(s) | 50, 156, 242, 248, 263, 442, 577, 705 |
| HapII | CCGG | 4 cut(s) | 71, 320, 369, 574 |
| HhaI | GCGC | 4 cut(s) | 92, 231, 374, 670 |
| Hin6I | GCGC | 4 cut(s) | 90, 229, 372, 668 |
| HinP1I | GCGC | 4 cut(s) | 90, 229, 372, 668 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HinfI | GANTC | 2 cut(s) | 482, 653 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 4 cut(s) | 71, 320, 369, 574 |
| HphI | GGTGA | 2 cut(s) | 157, 708 |
| Hpy166II | GTNNAC | 4 cut(s) | 79, 85, 112, 221 |
| Hpy188III | TCNNGA | 2 cut(s) | 339, 546 |
| Hpy8I | GTNNAC | 4 cut(s) | 79, 85, 112, 221 |
| HpyCH4III | ACNGT | 1 cut(s) | 336 |
| HpyCH4IV | ACGT | 4 cut(s) | 81, 217, 266, 277 |
| HpyCH4V | TGCA | 4 cut(s) | 403, 493, 596, 622 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 153, 320, 409, 588, 593, 631 |
| HpyF3I | CTNAG | 2 cut(s) | 290, 326 |
| HpySE526I | ACGT | 4 cut(s) | 81, 217, 266, 277 |
| HspAI | GCGC | 4 cut(s) | 90, 229, 372, 668 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 2 cut(s) | 193, 554 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 1 cut(s) | 631 |
| Lsp1109I | GCAGC | 2 cut(s) | 153, 634 |
| LweI | GCATC | 2 cut(s) | 583, 609 |
| MaeI | CTAG | 1 cut(s) | 363 |
| MaeII | ACGT | 4 cut(s) | 81, 217, 266, 277 |
| MaeIII | GTNAC | 2 cut(s) | 273, 336 |
| MalI | GATC | 2 cut(s) | 195, 556 |
| MbiI | CCGCTC | 2 cut(s) | 602, 650 |
| MboI | GATC | 2 cut(s) | 193, 554 |
| MboII | GAAGA | 3 cut(s) | 41, 188, 306 |
| MflI | RGATCY | 1 cut(s) | 554 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluCI | AATT | 2 cut(s) | 642, 672 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MlyI | GAGTC | 1 cut(s) | 662 |
| MmeI | TCCRAC | 1 cut(s) | 609 |
| MnlI | CCTC | 8 cut(s) | 173, 178, 238, 277, 298, 469, 520, 545 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MseI | TTAA | 3 cut(s) | 84, 461, 641 |
| MslI | CAYNNNNRTG | 2 cut(s) | 107, 269 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 4 cut(s) | 412, 565, 587, 721 |
| MspI | CCGG | 4 cut(s) | 71, 320, 369, 574 |
| MspR9I | CCNGG | 8 cut(s) | 71, 117, 153, 320, 349, 574, 702, 707 |
| Mva1269I | GAATGC | 1 cut(s) | 574 |
| MvaI | CCWGG | 5 cut(s) | 117, 153, 349, 702, 707 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 153, 320, 409, 588, 593, 631 |
| NciI | CCSGG | 3 cut(s) | 71, 320, 574 |
| NdeI | CATATG | 1 cut(s) | 399 |
| NdeII | GATC | 2 cut(s) | 193, 554 |
| NlaIV | GGNNCC | 4 cut(s) | 408, 417, 441, 661 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 2 cut(s) | 273, 336 |
| OliI | CACNNNNGTG | 2 cut(s) | 107, 269 |
| PaqCI | CACCTGC | 1 cut(s) | 247 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PctI | GAATGC | 1 cut(s) | 574 |
| PfeI | GAWTC | 1 cut(s) | 482 |
| PkrI | GCNGC | 4 cut(s) | 143, 146, 444, 624 |
| PleI | GAGTC | 1 cut(s) | 661 |
| PpsI | GAGTC | 1 cut(s) | 661 |
| PpuMI | RGGWCCY | 1 cut(s) | 406 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp5II | RGGWCCY | 1 cut(s) | 406 |
| Psp6I | CCWGG | 5 cut(s) | 115, 151, 347, 700, 705 |
| PspFI | CCCAGC | 1 cut(s) | 664 |
| PspGI | CCWGG | 5 cut(s) | 115, 151, 347, 700, 705 |
| PspN4I | GGNNCC | 4 cut(s) | 408, 417, 441, 661 |
| PspPI | GGNCC | 6 cut(s) | 247, 406, 415, 440, 576, 709 |
| PspPPI | RGGWCCY | 1 cut(s) | 406 |
| PsuI | RGATCY | 1 cut(s) | 554 |
| RsaI | GTAC | 2 cut(s) | 367, 453 |
| RsaNI | GTAC | 2 cut(s) | 366, 452 |
| RseI | CAYNNNNRTG | 2 cut(s) | 107, 269 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 3 cut(s) | 84, 461, 641 |
| SatI | GCNGC | 4 cut(s) | 142, 145, 443, 623 |
| Sau3AI | GATC | 2 cut(s) | 193, 554 |
| Sau96I | GGNCC | 6 cut(s) | 247, 406, 415, 440, 576, 709 |
| SchI | GAGTC | 1 cut(s) | 662 |
| ScrFI | CCNGG | 8 cut(s) | 71, 117, 153, 320, 349, 574, 702, 707 |
| SfaNI | GCATC | 2 cut(s) | 583, 609 |
| SinI | GGWCC | 3 cut(s) | 406, 415, 709 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 107, 269 |
| SmlI | CTYRAG | 1 cut(s) | 505 |
| SmoI | CTYRAG | 1 cut(s) | 505 |
| Sse9I | AATT | 2 cut(s) | 642, 672 |
| SspMI | CTAG | 1 cut(s) | 363 |
| StyD4I | CCNGG | 8 cut(s) | 69, 115, 151, 318, 347, 572, 700, 705 |
| StyI | CCWWGG | 1 cut(s) | 512 |
| TaaI | ACNGT | 1 cut(s) | 336 |
| TaiI | ACGT | 4 cut(s) | 84, 220, 269, 280 |
| TaqI | TCGA | 1 cut(s) | 646 |
| TasI | AATT | 2 cut(s) | 642, 672 |
| TauI | GCSGC | 2 cut(s) | 147, 445 |
| TfiI | GAWTC | 1 cut(s) | 482 |
| Tru1I | TTAA | 3 cut(s) | 84, 461, 641 |
| Tru9I | TTAA | 3 cut(s) | 84, 461, 641 |
| TscAI | CASTG | 4 cut(s) | 114, 192, 330, 495 |
| TseFI | GTSAC | 2 cut(s) | 273, 336 |
| TseI | GCWGC | 2 cut(s) | 141, 622 |
| Tsp45I | GTSAC | 2 cut(s) | 273, 336 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspGWI | ACGGA | 1 cut(s) | 218 |
| TspRI | CASTG | 4 cut(s) | 114, 192, 330, 495 |
| VpaK11BI | GGWCC | 3 cut(s) | 406, 415, 709 |
| XspI | CTAG | 1 cut(s) | 363 |