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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 218 |
| Acc36I | ACCTGC | 1 cut(s) | 235 |
| AccBSI | CCGCTC | 1 cut(s) | 530 |
| AccI | GTMKAC | 1 cut(s) | 346 |
| AciI | CCGC | 8 cut(s) | 38, 155, 179, 410, 430, 495, 528, 559 |
| AclI | AACGTT | 1 cut(s) | 81 |
| AclWI | GGATC | 1 cut(s) | 48 |
| AcsI | RAATTY | 1 cut(s) | 266 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 2 cut(s) | 369, 375 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 190, 244, 356, 455 |
| AgsI | TTSAA | 1 cut(s) | 100 |
| AjnI | CCWGG | 6 cut(s) | 138, 143, 418, 454, 540, 545 |
| AluBI | AGCT | 1 cut(s) | 168 |
| AluI | AGCT | 1 cut(s) | 168 |
| Alw21I | GWGCWC | 2 cut(s) | 94, 335 |
| Alw26I | GTCTC | 1 cut(s) | 492 |
| AlwI | GGATC | 1 cut(s) | 48 |
| AoxI | GGCC | 5 cut(s) | 141, 228, 403, 543, 574 |
| ApeKI | GCWGC | 2 cut(s) | 125, 467 |
| ApoI | RAATTY | 1 cut(s) | 266 |
| AspLEI | GCGC | 5 cut(s) | 219, 289, 313, 507, 515 |
| AspS9I | GGNCC | 3 cut(s) | 181, 426, 549 |
| AsuHPI | GGTGA | 1 cut(s) | 548 |
| AvaII | GGWCC | 3 cut(s) | 181, 426, 549 |
| Bbv12I | GWGCWC | 2 cut(s) | 94, 335 |
| BbvI | GCAGC | 2 cut(s) | 137, 479 |
| BccI | CCATC | 1 cut(s) | 66 |
| BceAI | ACGGC | 1 cut(s) | 561 |
| BciT130I | CCWGG | 6 cut(s) | 140, 145, 420, 456, 542, 547 |
| BcoDI | GTCTC | 1 cut(s) | 492 |
| BfaI | CTAG | 2 cut(s) | 107, 407 |
| BfmI | CTRYAG | 2 cut(s) | 442, 570 |
| BfoI | RGCGCY | 3 cut(s) | 290, 508, 516 |
| BfuAI | ACCTGC | 1 cut(s) | 235 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 140 |
| BisI | GCNGC | 3 cut(s) | 126, 155, 468 |
| BlsI | GCNGC | 3 cut(s) | 127, 156, 469 |
| Bme1390I | CCNGG | 6 cut(s) | 140, 145, 420, 456, 542, 547 |
| Bme18I | GGWCC | 3 cut(s) | 181, 426, 549 |
| BmgT120I | GGNCC | 3 cut(s) | 181, 426, 549 |
| BmiI | GGNNCC | 2 cut(s) | 427, 428 |
| BmrFI | CCNGG | 6 cut(s) | 140, 145, 420, 456, 542, 547 |
| BmsI | GCATC | 3 cut(s) | 378, 428, 454 |
| BpmI | CTGGAG | 2 cut(s) | 311, 402 |
| BsaJI | CCNNGG | 1 cut(s) | 72 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 190, 244, 356, 455 |
| BseBI | CCWGG | 6 cut(s) | 140, 145, 420, 456, 542, 547 |
| BseDI | CCNNGG | 1 cut(s) | 72 |
| BseGI | GGATG | 3 cut(s) | 58, 443, 469 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 190, 244, 356, 455 |
| BseXI | GCAGC | 2 cut(s) | 137, 479 |
| BseYI | CCCAGC | 3 cut(s) | 121, 307, 432 |
| BshFI | GGCC | 5 cut(s) | 143, 230, 405, 545, 576 |
| BsiHKAI | GWGCWC | 2 cut(s) | 94, 335 |
| BslFI | GGGAC | 1 cut(s) | 439 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 190, 244, 356, 455 |
| BsmAI | GTCTC | 1 cut(s) | 492 |
| BsmFI | GGGAC | 1 cut(s) | 439 |
| BsnI | GGCC | 5 cut(s) | 143, 230, 405, 545, 576 |
| Bsp1286I | GDGCHC | 2 cut(s) | 94, 335 |
| Bsp143I | GATC | 1 cut(s) | 40 |
| BspACI | CCGC | 8 cut(s) | 38, 155, 179, 410, 430, 495, 528, 559 |
| BspANI | GGCC | 5 cut(s) | 143, 230, 405, 545, 576 |
| BspLI | GGNNCC | 2 cut(s) | 427, 428 |
| BspMAI | CTGCAG | 1 cut(s) | 574 |
| BspMI | ACCTGC | 1 cut(s) | 235 |
| BspPI | GGATC | 1 cut(s) | 48 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BsrBI | CCGCTC | 1 cut(s) | 530 |
| BssECI | CCNNGG | 1 cut(s) | 72 |
| BssMI | GATC | 1 cut(s) | 40 |
| Bst2UI | CCWGG | 6 cut(s) | 140, 145, 420, 456, 542, 547 |
| Bst4CI | ACNGT | 2 cut(s) | 11, 446 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 4 cut(s) | 36, 170, 304, 574 |
| BstDEI | CTNAG | 1 cut(s) | 491 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 188 |
| BstF5I | GGATG | 3 cut(s) | 58, 443, 469 |
| BstH2I | RGCGCY | 3 cut(s) | 290, 508, 516 |
| BstHHI | GCGC | 5 cut(s) | 219, 289, 313, 507, 515 |
| BstKTI | GATC | 1 cut(s) | 43 |
| BstMAI | GTCTC | 1 cut(s) | 492 |
| BstMBI | GATC | 1 cut(s) | 40 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 131, 140, 293, 308, 319, 438, 476 |
| BstNI | CCWGG | 6 cut(s) | 140, 145, 420, 456, 542, 547 |
| BstSCI | CCNGG | 6 cut(s) | 138, 143, 418, 454, 540, 545 |
| BstSFI | CTRYAG | 2 cut(s) | 442, 570 |
| BstV1I | GCAGC | 2 cut(s) | 137, 479 |
| BsuRI | GGCC | 5 cut(s) | 143, 230, 405, 545, 576 |
| BtsCI | GGATG | 3 cut(s) | 58, 443, 469 |
| BveI | ACCTGC | 1 cut(s) | 235 |
| Cac8I | GCNNGC | 4 cut(s) | 36, 170, 304, 574 |
| CfoI | GCGC | 5 cut(s) | 219, 289, 313, 507, 515 |
| Cfr13I | GGNCC | 3 cut(s) | 181, 426, 549 |
| CseI | GACGC | 1 cut(s) | 526 |
| Csp6I | GTAC | 2 cut(s) | 368, 374 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 340, 375 |
| CviJI | RGCY | 8 cut(s) | 143, 168, 230, 236, 405, 485, 545, 576 |
| CviKI_1 | RGCY | 8 cut(s) | 143, 168, 230, 236, 405, 485, 545, 576 |
| CviQI | GTAC | 2 cut(s) | 368, 374 |
| DdeI | CTNAG | 1 cut(s) | 491 |
| DpnI | GATC | 1 cut(s) | 42 |
| DpnII | GATC | 1 cut(s) | 40 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| Eco47I | GGWCC | 3 cut(s) | 181, 426, 549 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 188 |
| EcoO109I | RGGNCCY | 1 cut(s) | 426 |
| EcoRII | CCWGG | 6 cut(s) | 138, 143, 418, 454, 540, 545 |
| FaiI | YATR | 3 cut(s) | 160, 164, 383 |
| FaqI | GGGAC | 1 cut(s) | 439 |
| FauI | CCCGC | 2 cut(s) | 437, 535 |
| FblI | GTMKAC | 1 cut(s) | 346 |
| Fnu4HI | GCNGC | 3 cut(s) | 126, 155, 468 |
| FokI | GGATG | 3 cut(s) | 45, 450, 476 |
| Fsp4HI | GCNGC | 3 cut(s) | 126, 155, 468 |
| FspBI | CTAG | 2 cut(s) | 107, 407 |
| FspI | TGCGCA | 1 cut(s) | 218 |
| GlaI | GCGC | 5 cut(s) | 218, 288, 312, 506, 514 |
| GluI | GCNGC | 3 cut(s) | 126, 155, 468 |
| GsaI | CCCAGC | 3 cut(s) | 125, 311, 436 |
| GsuI | CTGGAG | 2 cut(s) | 311, 402 |
| HaeII | RGCGCY | 3 cut(s) | 290, 508, 516 |
| HaeIII | GGCC | 5 cut(s) | 143, 230, 405, 545, 576 |
| HgaI | GACGC | 1 cut(s) | 526 |
| HhaI | GCGC | 5 cut(s) | 219, 289, 313, 507, 515 |
| Hin6I | GCGC | 5 cut(s) | 217, 287, 311, 505, 513 |
| HinP1I | GCGC | 5 cut(s) | 217, 287, 311, 505, 513 |
| HincII | GTYRAC | 4 cut(s) | 79, 85, 112, 347 |
| HindII | GTYRAC | 4 cut(s) | 79, 85, 112, 347 |
| HinfI | GANTC | 2 cut(s) | 413, 565 |
| HpaI | GTTAAC | 3 cut(s) | 79, 85, 112 |
| HphI | GGTGA | 1 cut(s) | 548 |
| Hpy166II | GTNNAC | 5 cut(s) | 79, 85, 112, 209, 347 |
| Hpy8I | GTNNAC | 5 cut(s) | 79, 85, 112, 209, 347 |
| HpyAV | CCTTC | 1 cut(s) | 220 |
| HpyCH4III | ACNGT | 2 cut(s) | 11, 446 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 340 |
| HpyCH4V | TGCA | 3 cut(s) | 391, 467, 572 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 131, 140, 293, 308, 319, 438, 476 |
| HpyF3I | CTNAG | 1 cut(s) | 491 |
| HpySE526I | ACGT | 2 cut(s) | 81, 340 |
| HspAI | GCGC | 5 cut(s) | 217, 287, 311, 505, 513 |
| KflI | GGGWCCC | 1 cut(s) | 426 |
| KspAI | GTTAAC | 3 cut(s) | 79, 85, 112 |
| Kzo9I | GATC | 1 cut(s) | 40 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 2 cut(s) | 202, 330 |
| Lsp1109I | GCAGC | 2 cut(s) | 137, 479 |
| LweI | GCATC | 3 cut(s) | 378, 428, 454 |
| MaeI | CTAG | 2 cut(s) | 107, 407 |
| MaeII | ACGT | 2 cut(s) | 81, 340 |
| MalI | GATC | 1 cut(s) | 42 |
| MbiI | CCGCTC | 1 cut(s) | 530 |
| MboI | GATC | 1 cut(s) | 40 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MhlI | GDGCHC | 2 cut(s) | 94, 335 |
| MluCI | AATT | 3 cut(s) | 266, 471, 517 |
| MnlI | CCTC | 7 cut(s) | 10, 82, 194, 276, 286, 392, 416 |
| MseI | TTAA | 4 cut(s) | 78, 84, 111, 278 |
| MslI | CAYNNNNRTG | 1 cut(s) | 386 |
| MspA1I | CMGCKG | 1 cut(s) | 432 |
| MspR9I | CCNGG | 6 cut(s) | 140, 145, 420, 456, 542, 547 |
| MvaI | CCWGG | 6 cut(s) | 140, 145, 420, 456, 542, 547 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 131, 140, 293, 308, 319, 438, 476 |
| NdeII | GATC | 1 cut(s) | 40 |
| NlaIV | GGNNCC | 2 cut(s) | 427, 428 |
| NsbI | TGCGCA | 1 cut(s) | 218 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PfeI | GAWTC | 2 cut(s) | 413, 565 |
| PflFI | GACNNNGTC | 1 cut(s) | 342 |
| PfoI | TCCNGGA | 2 cut(s) | 418, 454 |
| PkrI | GCNGC | 3 cut(s) | 127, 156, 469 |
| PpuMI | RGGWCCY | 1 cut(s) | 426 |
| Psp1406I | AACGTT | 1 cut(s) | 81 |
| Psp5II | RGGWCCY | 1 cut(s) | 426 |
| Psp6I | CCWGG | 6 cut(s) | 138, 143, 418, 454, 540, 545 |
| PspFI | CCCAGC | 3 cut(s) | 121, 307, 432 |
| PspGI | CCWGG | 6 cut(s) | 138, 143, 418, 454, 540, 545 |
| PspN4I | GGNNCC | 2 cut(s) | 427, 428 |
| PspPI | GGNCC | 3 cut(s) | 181, 426, 549 |
| PspPPI | RGGWCCY | 1 cut(s) | 426 |
| PstI | CTGCAG | 1 cut(s) | 574 |
| PsyI | GACNNNGTC | 1 cut(s) | 342 |
| RsaI | GTAC | 2 cut(s) | 369, 375 |
| RsaNI | GTAC | 2 cut(s) | 368, 374 |
| RseI | CAYNNNNRTG | 1 cut(s) | 386 |
| SalI | GTCGAC | 1 cut(s) | 345 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 4 cut(s) | 78, 84, 111, 278 |
| SatI | GCNGC | 3 cut(s) | 126, 155, 468 |
| Sau3AI | GATC | 1 cut(s) | 40 |
| Sau96I | GGNCC | 3 cut(s) | 181, 426, 549 |
| SbfI | CCTGCAGG | 1 cut(s) | 574 |
| ScrFI | CCNGG | 6 cut(s) | 140, 145, 420, 456, 542, 547 |
| SdaI | CCTGCAGG | 1 cut(s) | 574 |
| SduI | GDGCHC | 2 cut(s) | 94, 335 |
| SetI | ASST | 6 cut(s) | 84, 170, 186, 249, 343, 403 |
| SfaNI | GCATC | 3 cut(s) | 378, 428, 454 |
| SfcI | CTRYAG | 2 cut(s) | 442, 570 |
| SinI | GGWCC | 3 cut(s) | 181, 426, 549 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 386 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 574 |
| Sse9I | AATT | 3 cut(s) | 266, 471, 517 |
| SsiI | CCGC | 8 cut(s) | 38, 155, 179, 410, 430, 495, 528, 559 |
| SspI | AATATT | 1 cut(s) | 355 |
| SspMI | CTAG | 2 cut(s) | 107, 407 |
| StyD4I | CCNGG | 6 cut(s) | 138, 143, 418, 454, 540, 545 |
| TaaI | ACNGT | 2 cut(s) | 11, 446 |
| TaiI | ACGT | 2 cut(s) | 84, 343 |
| TaqI | TCGA | 1 cut(s) | 346 |
| TasI | AATT | 3 cut(s) | 266, 471, 517 |
| TauI | GCSGC | 1 cut(s) | 157 |
| TfiI | GAWTC | 2 cut(s) | 413, 565 |
| Tru1I | TTAA | 4 cut(s) | 78, 84, 111, 278 |
| Tru9I | TTAA | 4 cut(s) | 78, 84, 111, 278 |
| TseI | GCWGC | 2 cut(s) | 125, 467 |
| Tth111I | GACNNNGTC | 1 cut(s) | 342 |
| VpaK11BI | GGWCC | 3 cut(s) | 181, 426, 549 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 188 |
| XapI | RAATTY | 1 cut(s) | 266 |
| XmiI | GTMKAC | 1 cut(s) | 346 |
| XspI | CTAG | 2 cut(s) | 107, 407 |