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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 380 |
| AccBSI | CCGCTC | 3 cut(s) | 490, 528, 588 |
| AccII | CGCG | 2 cut(s) | 340, 678 |
| AclWI | GGATC | 3 cut(s) | 201, 548, 623 |
| AcoI | YGGCCR | 2 cut(s) | 261, 715 |
| AdeI | CACNNNGTG | 1 cut(s) | 329 |
| AfaI | GTAC | 2 cut(s) | 439, 557 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 283, 354, 532 |
| AgsI | TTSAA | 1 cut(s) | 664 |
| AjnI | CCWGG | 7 cut(s) | 133, 151, 318, 347, 558, 681, 686 |
| AleI | CACNNNNGTG | 1 cut(s) | 269 |
| AluBI | AGCT | 3 cut(s) | 181, 289, 446 |
| AluI | AGCT | 3 cut(s) | 181, 289, 446 |
| Alw26I | GTCTC | 3 cut(s) | 119, 359, 488 |
| AlwI | GGATC | 3 cut(s) | 201, 548, 623 |
| Ama87I | CYCGRG | 2 cut(s) | 491, 499 |
| AoxI | GGCC | 6 cut(s) | 154, 240, 246, 261, 684, 715 |
| ApeKI | GCWGC | 2 cut(s) | 398, 702 |
| AspS9I | GGNCC | 4 cut(s) | 247, 392, 426, 690 |
| AsuC2I | CCSGG | 3 cut(s) | 107, 117, 633 |
| AsuHPI | GGTGA | 3 cut(s) | 91, 134, 689 |
| AvaI | CYCGRG | 2 cut(s) | 491, 499 |
| AvaII | GGWCC | 3 cut(s) | 392, 426, 690 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| BbsI | GAAGAC | 2 cut(s) | 27, 744 |
| BbvI | GCAGC | 2 cut(s) | 385, 689 |
| BceAI | ACGGC | 1 cut(s) | 702 |
| BciT130I | CCWGG | 7 cut(s) | 135, 153, 320, 349, 560, 683, 688 |
| BcnI | CCSGG | 3 cut(s) | 107, 117, 633 |
| BcoDI | GTCTC | 3 cut(s) | 119, 359, 488 |
| BfaI | CTAG | 2 cut(s) | 188, 564 |
| BfuAI | ACCTGC | 1 cut(s) | 380 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 320 |
| BisI | GCNGC | 2 cut(s) | 399, 703 |
| BlsI | GCNGC | 2 cut(s) | 400, 704 |
| Bme18I | GGWCC | 3 cut(s) | 392, 426, 690 |
| BmeT110I | CYCGRG | 2 cut(s) | 491, 499 |
| BmgT120I | GGNCC | 4 cut(s) | 247, 392, 426, 690 |
| BmiI | GGNNCC | 3 cut(s) | 394, 427, 647 |
| BmsI | GCATC | 2 cut(s) | 569, 595 |
| BpiI | GAAGAC | 2 cut(s) | 27, 744 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| BpuMI | CCSGG | 3 cut(s) | 107, 117, 633 |
| BsaI | GGTCTC | 2 cut(s) | 359, 488 |
| BsaJI | CCNNGG | 5 cut(s) | 105, 115, 133, 243, 498 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 453, 483 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 283, 354, 532 |
| Bse118I | RCCGGY | 2 cut(s) | 573, 725 |
| Bse1I | ACTGG | 3 cut(s) | 43, 375, 601 |
| BseBI | CCWGG | 7 cut(s) | 135, 153, 320, 349, 560, 683, 688 |
| BseDI | CCNNGG | 5 cut(s) | 105, 115, 133, 243, 498 |
| BseGI | GGATG | 1 cut(s) | 610 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 283, 354, 532 |
| BseNI | ACTGG | 3 cut(s) | 43, 375, 601 |
| BseRI | GAGGAG | 1 cut(s) | 299 |
| BseXI | GCAGC | 2 cut(s) | 385, 689 |
| BseYI | CCCAGC | 1 cut(s) | 442 |
| BsgI | GTGCAG | 2 cut(s) | 384, 462 |
| Bsh1236I | CGCG | 2 cut(s) | 340, 678 |
| BshFI | GGCC | 6 cut(s) | 156, 242, 248, 263, 686, 717 |
| BsiHKCI | CYCGRG | 2 cut(s) | 491, 499 |
| BsiSI | CCGG | 5 cut(s) | 107, 117, 574, 632, 726 |
| BslFI | GGGAC | 3 cut(s) | 259, 378, 439 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 283, 354, 532 |
| BsmAI | GTCTC | 3 cut(s) | 119, 359, 488 |
| BsmFI | GGGAC | 3 cut(s) | 259, 378, 439 |
| BsnI | GGCC | 6 cut(s) | 156, 242, 248, 263, 686, 717 |
| Bso31I | GGTCTC | 2 cut(s) | 359, 488 |
| BsoBI | CYCGRG | 2 cut(s) | 491, 499 |
| Bsp143I | GATC | 3 cut(s) | 193, 540, 628 |
| Bsp68I | TCGCGA | 1 cut(s) | 340 |
| BspANI | GGCC | 6 cut(s) | 156, 242, 248, 263, 686, 717 |
| BspFNI | CGCG | 2 cut(s) | 340, 678 |
| BspLI | GGNNCC | 3 cut(s) | 394, 427, 647 |
| BspMI | ACCTGC | 1 cut(s) | 380 |
| BspPI | GGATC | 3 cut(s) | 201, 548, 623 |
| BspTNI | GGTCTC | 2 cut(s) | 359, 488 |
| BsrBI | CCGCTC | 3 cut(s) | 490, 528, 588 |
| BsrFI | RCCGGY | 2 cut(s) | 573, 725 |
| BsrI | ACTGG | 3 cut(s) | 43, 375, 601 |
| BssAI | RCCGGY | 2 cut(s) | 573, 725 |
| BssECI | CCNNGG | 5 cut(s) | 105, 115, 133, 243, 498 |
| BssMI | GATC | 3 cut(s) | 193, 540, 628 |
| Bst2UI | CCWGG | 7 cut(s) | 135, 153, 320, 349, 560, 683, 688 |
| Bst4CI | ACNGT | 2 cut(s) | 76, 613 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 149, 183, 575, 715 |
| BstF5I | GGATG | 1 cut(s) | 610 |
| BstFNI | CGCG | 2 cut(s) | 340, 678 |
| BstKTI | GATC | 3 cut(s) | 196, 543, 631 |
| BstMAI | GTCTC | 3 cut(s) | 119, 359, 488 |
| BstMBI | GATC | 3 cut(s) | 193, 540, 628 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 153, 320, 395, 579 |
| BstNI | CCWGG | 7 cut(s) | 135, 153, 320, 349, 560, 683, 688 |
| BstUI | CGCG | 2 cut(s) | 340, 678 |
| BstV1I | GCAGC | 2 cut(s) | 385, 689 |
| BstV2I | GAAGAC | 2 cut(s) | 27, 744 |
| BstX2I | RGATCY | 1 cut(s) | 193 |
| BstYI | RGATCY | 1 cut(s) | 193 |
| BsuRI | GGCC | 6 cut(s) | 156, 242, 248, 263, 686, 717 |
| BtgZI | GCGATG | 1 cut(s) | 592 |
| BtsCI | GGATG | 1 cut(s) | 610 |
| BtsI | GCAGTG | 1 cut(s) | 474 |
| BtsIMutI | CAGTG | 1 cut(s) | 474 |
| BtuMI | TCGCGA | 1 cut(s) | 340 |
| BveI | ACCTGC | 1 cut(s) | 380 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 149, 183, 575, 715 |
| Cfr10I | RCCGGY | 2 cut(s) | 573, 725 |
| Cfr13I | GGNCC | 4 cut(s) | 247, 392, 426, 690 |
| CseI | GACGC | 1 cut(s) | 667 |
| Csp6I | GTAC | 2 cut(s) | 438, 556 |
| CviAII | CATG | 1 cut(s) | 224 |
| CviQI | GTAC | 2 cut(s) | 438, 556 |
| DpnI | GATC | 3 cut(s) | 195, 542, 630 |
| DpnII | GATC | 3 cut(s) | 193, 540, 628 |
| DraIII | CACNNNGTG | 1 cut(s) | 329 |
| EaeI | YGGCCR | 2 cut(s) | 261, 715 |
| EciI | GGCGGA | 2 cut(s) | 37, 651 |
| Eco31I | GGTCTC | 2 cut(s) | 359, 488 |
| Eco47I | GGWCC | 3 cut(s) | 392, 426, 690 |
| Eco88I | CYCGRG | 2 cut(s) | 491, 499 |
| EcoO109I | RGGNCCY | 1 cut(s) | 392 |
| EcoRII | CCWGG | 7 cut(s) | 133, 151, 318, 347, 558, 681, 686 |
| FaeI | CATG | 1 cut(s) | 227 |
| FaiI | YATR | 6 cut(s) | 177, 201, 225, 385, 387, 733 |
| FaqI | GGGAC | 3 cut(s) | 259, 378, 439 |
| FatI | CATG | 1 cut(s) | 223 |
| FauI | CCCGC | 5 cut(s) | 8, 284, 403, 533, 578 |
| FauNDI | CATATG | 1 cut(s) | 385 |
| Fnu4HI | GCNGC | 2 cut(s) | 399, 703 |
| FokI | GGATG | 1 cut(s) | 617 |
| Fsp4HI | GCNGC | 2 cut(s) | 399, 703 |
| FspBI | CTAG | 2 cut(s) | 188, 564 |
| GluI | GCNGC | 2 cut(s) | 399, 703 |
| GsaI | CCCAGC | 1 cut(s) | 446 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HaeIII | GGCC | 6 cut(s) | 156, 242, 248, 263, 686, 717 |
| HapII | CCGG | 5 cut(s) | 107, 117, 574, 632, 726 |
| HgaI | GACGC | 1 cut(s) | 667 |
| Hin1II | CATG | 1 cut(s) | 227 |
| HincII | GTYRAC | 2 cut(s) | 28, 112 |
| HindII | GTYRAC | 2 cut(s) | 28, 112 |
| HinfI | GANTC | 3 cut(s) | 468, 639, 740 |
| HpaII | CCGG | 5 cut(s) | 107, 117, 574, 632, 726 |
| HphI | GGTGA | 3 cut(s) | 91, 134, 689 |
| Hpy166II | GTNNAC | 3 cut(s) | 28, 112, 221 |
| Hpy188I | TCNGA | 1 cut(s) | 723 |
| Hpy188III | TCNNGA | 3 cut(s) | 339, 493, 643 |
| Hpy8I | GTNNAC | 3 cut(s) | 28, 112, 221 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyCH4III | ACNGT | 2 cut(s) | 76, 613 |
| HpyCH4IV | ACGT | 1 cut(s) | 24 |
| HpyCH4V | TGCA | 7 cut(s) | 383, 389, 401, 479, 582, 608, 705 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 153, 320, 395, 579 |
| HpySE526I | ACGT | 1 cut(s) | 24 |
| Hsp92II | CATG | 1 cut(s) | 227 |
| KroI | GCCGGC | 1 cut(s) | 573 |
| KroNI | GCCGGC | 1 cut(s) | 575 |
| Kzo9I | GATC | 3 cut(s) | 193, 540, 628 |
| LmnI | GCTCC | 2 cut(s) | 286, 357 |
| Lsp1109I | GCAGC | 2 cut(s) | 385, 689 |
| LweI | GCATC | 2 cut(s) | 569, 595 |
| MaeI | CTAG | 2 cut(s) | 188, 564 |
| MaeII | ACGT | 1 cut(s) | 24 |
| MaeIII | GTNAC | 1 cut(s) | 751 |
| MalI | GATC | 3 cut(s) | 195, 542, 630 |
| MbiI | CCGCTC | 3 cut(s) | 490, 528, 588 |
| MboI | GATC | 3 cut(s) | 193, 540, 628 |
| MboII | GAAGA | 3 cut(s) | 32, 188, 749 |
| MflI | RGATCY | 1 cut(s) | 193 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluCI | AATT | 2 cut(s) | 411, 706 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MlyI | GAGTC | 2 cut(s) | 477, 734 |
| MmeI | TCCRAC | 1 cut(s) | 595 |
| MnlI | CCTC | 9 cut(s) | 173, 178, 238, 277, 298, 455, 506, 508, 531 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MreI | CGCCGGCG | 1 cut(s) | 573 |
| MroNI | GCCGGC | 1 cut(s) | 573 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 269 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 4 cut(s) | 398, 551, 702, 763 |
| MspI | CCGG | 5 cut(s) | 107, 117, 574, 632, 726 |
| MvaI | CCWGG | 7 cut(s) | 135, 153, 320, 349, 560, 683, 688 |
| MvnI | CGCG | 2 cut(s) | 340, 678 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 153, 320, 395, 579 |
| NaeI | GCCGGC | 1 cut(s) | 575 |
| NciI | CCSGG | 3 cut(s) | 107, 117, 633 |
| NdeI | CATATG | 1 cut(s) | 385 |
| NdeII | GATC | 3 cut(s) | 193, 540, 628 |
| NgoMIV | GCCGGC | 1 cut(s) | 573 |
| NlaIII | CATG | 1 cut(s) | 227 |
| NlaIV | GGNNCC | 3 cut(s) | 394, 427, 647 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 1 cut(s) | 751 |
| NruI | TCGCGA | 1 cut(s) | 340 |
| OliI | CACNNNNGTG | 1 cut(s) | 269 |
| PaeR7I | CTCGAG | 1 cut(s) | 491 |
| PdiI | GCCGGC | 1 cut(s) | 575 |
| PfeI | GAWTC | 1 cut(s) | 639 |
| PkrI | GCNGC | 2 cut(s) | 400, 704 |
| PleI | GAGTC | 2 cut(s) | 476, 734 |
| PpsI | GAGTC | 2 cut(s) | 476, 734 |
| PpuMI | RGGWCCY | 1 cut(s) | 392 |
| Psp5II | RGGWCCY | 1 cut(s) | 392 |
| Psp6I | CCWGG | 7 cut(s) | 133, 151, 318, 347, 558, 681, 686 |
| PspFI | CCCAGC | 1 cut(s) | 442 |
| PspGI | CCWGG | 7 cut(s) | 133, 151, 318, 347, 558, 681, 686 |
| PspN4I | GGNNCC | 3 cut(s) | 394, 427, 647 |
| PspPI | GGNCC | 4 cut(s) | 247, 392, 426, 690 |
| PspPPI | RGGWCCY | 1 cut(s) | 392 |
| PsuI | RGATCY | 1 cut(s) | 193 |
| RruI | TCGCGA | 1 cut(s) | 340 |
| RsaI | GTAC | 2 cut(s) | 439, 557 |
| RsaNI | GTAC | 2 cut(s) | 438, 556 |
| RseI | CAYNNNNRTG | 1 cut(s) | 269 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 2 cut(s) | 399, 703 |
| Sau3AI | GATC | 3 cut(s) | 193, 540, 628 |
| Sau96I | GGNCC | 4 cut(s) | 247, 392, 426, 690 |
| SchI | GAGTC | 2 cut(s) | 477, 734 |
| SetI | ASST | 9 cut(s) | 27, 139, 165, 183, 291, 394, 448, 500, 627 |
| SfaNI | GCATC | 2 cut(s) | 569, 595 |
| Sfr274I | CTCGAG | 1 cut(s) | 491 |
| SgrAI | CRCCGGYG | 1 cut(s) | 573 |
| SinI | GGWCC | 3 cut(s) | 392, 426, 690 |
| SlaI | CTCGAG | 1 cut(s) | 491 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 269 |
| SmlI | CTYRAG | 1 cut(s) | 491 |
| SmoI | CTYRAG | 1 cut(s) | 491 |
| Sse9I | AATT | 2 cut(s) | 411, 706 |
| SspMI | CTAG | 2 cut(s) | 188, 564 |
| TaaI | ACNGT | 2 cut(s) | 76, 613 |
| TaiI | ACGT | 1 cut(s) | 27 |
| TaqI | TCGA | 1 cut(s) | 492 |
| TasI | AATT | 2 cut(s) | 411, 706 |
| TfiI | GAWTC | 1 cut(s) | 639 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 1 cut(s) | 481 |
| TseFI | GTSAC | 1 cut(s) | 751 |
| TseI | GCWGC | 2 cut(s) | 398, 702 |
| Tsp45I | GTSAC | 1 cut(s) | 751 |
| TspDTI | ATGAA | 2 cut(s) | 188, 240 |
| TspRI | CASTG | 1 cut(s) | 481 |
| VpaK11BI | GGWCC | 3 cut(s) | 392, 426, 690 |
| XhoI | CTCGAG | 1 cut(s) | 491 |
| XspI | CTAG | 2 cut(s) | 188, 564 |