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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 127 |
| AccBSI | CCGCTC | 1 cut(s) | 288 |
| AccII | CGCG | 1 cut(s) | 99 |
| AciI | CCGC | 8 cut(s) | 18, 25, 48, 71, 263, 275, 286, 349 |
| AcsI | RAATTY | 1 cut(s) | 158 |
| AcuI | CTGAAG | 1 cut(s) | 191 |
| AfeI | AGCGCT | 1 cut(s) | 180 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 37, 136, 248, 335 |
| AgeI | ACCGGT | 1 cut(s) | 248 |
| AluBI | AGCT | 3 cut(s) | 8, 13, 169 |
| AluI | AGCT | 3 cut(s) | 8, 13, 169 |
| Alw21I | GWGCWC | 1 cut(s) | 227 |
| Alw26I | GTCTC | 1 cut(s) | 369 |
| Alw44I | GTGCAC | 1 cut(s) | 223 |
| Aor51HI | AGCGCT | 1 cut(s) | 180 |
| AoxI | GGCC | 1 cut(s) | 126 |
| ApaLI | GTGCAC | 1 cut(s) | 223 |
| ApeKI | GCWGC | 1 cut(s) | 21 |
| ApoI | RAATTY | 1 cut(s) | 158 |
| AsiGI | ACCGGT | 1 cut(s) | 248 |
| AspA2I | CCTAGG | 1 cut(s) | 329 |
| AspLEI | GCGC | 3 cut(s) | 99, 111, 181 |
| AspS9I | GGNCC | 4 cut(s) | 40, 73, 127, 365 |
| AsuHPI | GGTGA | 1 cut(s) | 238 |
| AvaII | GGWCC | 3 cut(s) | 40, 73, 365 |
| AvrII | CCTAGG | 1 cut(s) | 329 |
| BaeGI | GKGCMC | 1 cut(s) | 227 |
| Bbv12I | GWGCWC | 1 cut(s) | 227 |
| BbvCI | CCTCAGC | 1 cut(s) | 123 |
| BbvI | GCAGC | 1 cut(s) | 33 |
| BccI | CCATC | 2 cut(s) | 251, 365 |
| BcoDI | GTCTC | 1 cut(s) | 369 |
| BfaI | CTAG | 5 cut(s) | 68, 228, 282, 303, 330 |
| BfoI | RGCGCY | 1 cut(s) | 182 |
| BfuAI | ACCTGC | 1 cut(s) | 127 |
| BisI | GCNGC | 6 cut(s) | 19, 22, 25, 48, 275, 286 |
| BlnI | CCTAGG | 1 cut(s) | 329 |
| BlpI | GCTNAGC | 2 cut(s) | 9, 14 |
| BlsI | GCNGC | 6 cut(s) | 20, 23, 26, 49, 276, 287 |
| Bme18I | GGWCC | 3 cut(s) | 40, 73, 365 |
| BmgT120I | GGNCC | 4 cut(s) | 40, 73, 127, 365 |
| BmiI | GGNNCC | 1 cut(s) | 366 |
| BmsI | GCATC | 1 cut(s) | 363 |
| Bpu10I | CCTNAGC | 1 cut(s) | 123 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 9, 14 |
| BsaJI | CCNNGG | 1 cut(s) | 329 |
| BsaWI | WCCGGW | 1 cut(s) | 248 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 370 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 37, 136, 248, 335 |
| Bse118I | RCCGGY | 1 cut(s) | 248 |
| Bse1I | ACTGG | 1 cut(s) | 66 |
| BseDI | CCNNGG | 1 cut(s) | 329 |
| BseGI | GGATG | 1 cut(s) | 354 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 37, 136, 248, 335 |
| BseMII | CTCAG | 3 cut(s) | 23, 114, 300 |
| BseNI | ACTGG | 1 cut(s) | 66 |
| BseSI | GKGCMC | 1 cut(s) | 227 |
| BseXI | GCAGC | 1 cut(s) | 33 |
| BseYI | CCCAGC | 1 cut(s) | 199 |
| Bsh1236I | CGCG | 1 cut(s) | 99 |
| BshFI | GGCC | 1 cut(s) | 128 |
| BshTI | ACCGGT | 1 cut(s) | 248 |
| BsiHKAI | GWGCWC | 1 cut(s) | 227 |
| BsiSI | CCGG | 1 cut(s) | 249 |
| BslFI | GGGAC | 2 cut(s) | 311, 378 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 37, 136, 248, 335 |
| BsmAI | GTCTC | 1 cut(s) | 369 |
| BsmFI | GGGAC | 2 cut(s) | 311, 378 |
| BsmI | GAATGC | 1 cut(s) | 196 |
| BsnI | GGCC | 1 cut(s) | 128 |
| Bsp1286I | GDGCHC | 1 cut(s) | 227 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 9, 14 |
| BspACI | CCGC | 8 cut(s) | 18, 25, 48, 71, 263, 275, 286, 349 |
| BspANI | GGCC | 1 cut(s) | 128 |
| BspCNI | CTCAG | 3 cut(s) | 22, 115, 301 |
| BspFNI | CGCG | 1 cut(s) | 99 |
| BspLI | GGNNCC | 1 cut(s) | 366 |
| BspMI | ACCTGC | 1 cut(s) | 127 |
| BsrBI | CCGCTC | 1 cut(s) | 288 |
| BsrFI | RCCGGY | 1 cut(s) | 248 |
| BsrI | ACTGG | 1 cut(s) | 66 |
| BssAI | RCCGGY | 1 cut(s) | 248 |
| BssECI | CCNNGG | 1 cut(s) | 329 |
| BssT1I | CCWWGG | 1 cut(s) | 329 |
| Bst4CI | ACNGT | 1 cut(s) | 216 |
| Bst6I | CTCTTC | 1 cut(s) | 180 |
| BstDEI | CTNAG | 4 cut(s) | 9, 14, 123, 309 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 333 |
| BstF5I | GGATG | 1 cut(s) | 354 |
| BstFNI | CGCG | 1 cut(s) | 99 |
| BstH2I | RGCGCY | 1 cut(s) | 182 |
| BstHHI | GCGC | 3 cut(s) | 99, 111, 181 |
| BstMAI | GTCTC | 1 cut(s) | 369 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 24, 200, 231, 346 |
| BstSLI | GKGCMC | 1 cut(s) | 227 |
| BstUI | CGCG | 1 cut(s) | 99 |
| BstV1I | GCAGC | 1 cut(s) | 33 |
| BsuRI | GGCC | 1 cut(s) | 128 |
| BtsCI | GGATG | 1 cut(s) | 354 |
| BtsI | GCAGTG | 1 cut(s) | 227 |
| BtsIMutI | CAGTG | 1 cut(s) | 227 |
| BveI | ACCTGC | 1 cut(s) | 127 |
| CfoI | GCGC | 3 cut(s) | 99, 111, 181 |
| Cfr10I | RCCGGY | 1 cut(s) | 248 |
| Cfr13I | GGNCC | 4 cut(s) | 40, 73, 127, 365 |
| CspAI | ACCGGT | 1 cut(s) | 248 |
| DdeI | CTNAG | 4 cut(s) | 9, 14, 123, 309 |
| Eam1104I | CTCTTC | 1 cut(s) | 180 |
| EarI | CTCTTC | 1 cut(s) | 180 |
| EciI | GGCGGA | 1 cut(s) | 278 |
| Eco130I | CCWWGG | 1 cut(s) | 329 |
| Eco47I | GGWCC | 3 cut(s) | 40, 73, 365 |
| Eco47III | AGCGCT | 1 cut(s) | 180 |
| Eco57I | CTGAAG | 1 cut(s) | 191 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 333 |
| EcoO109I | RGGNCCY | 1 cut(s) | 40 |
| EcoT14I | CCWWGG | 1 cut(s) | 329 |
| ErhI | CCWWGG | 1 cut(s) | 329 |
| FaiI | YATR | 3 cut(s) | 279, 320, 391 |
| FaqI | GGGAC | 2 cut(s) | 311, 378 |
| Fnu4HI | GCNGC | 6 cut(s) | 19, 22, 25, 48, 275, 286 |
| FokI | GGATG | 1 cut(s) | 341 |
| Fsp4HI | GCNGC | 6 cut(s) | 19, 22, 25, 48, 275, 286 |
| FspBI | CTAG | 5 cut(s) | 68, 228, 282, 303, 330 |
| GlaI | GCGC | 3 cut(s) | 98, 110, 180 |
| GluI | GCNGC | 6 cut(s) | 19, 22, 25, 48, 275, 286 |
| GsaI | CCCAGC | 1 cut(s) | 203 |
| HaeII | RGCGCY | 1 cut(s) | 182 |
| HaeIII | GGCC | 1 cut(s) | 128 |
| HapII | CCGG | 1 cut(s) | 249 |
| HhaI | GCGC | 3 cut(s) | 99, 111, 181 |
| Hin6I | GCGC | 3 cut(s) | 97, 109, 179 |
| HinP1I | GCGC | 3 cut(s) | 97, 109, 179 |
| HinfI | GANTC | 1 cut(s) | 266 |
| HpaII | CCGG | 1 cut(s) | 249 |
| HphI | GGTGA | 1 cut(s) | 238 |
| Hpy166II | GTNNAC | 1 cut(s) | 225 |
| Hpy188III | TCNNGA | 1 cut(s) | 358 |
| Hpy8I | GTNNAC | 1 cut(s) | 225 |
| HpyAV | CCTTC | 1 cut(s) | 355 |
| HpyCH4III | ACNGT | 1 cut(s) | 216 |
| HpyCH4V | TGCA | 2 cut(s) | 37, 225 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 24, 200, 231, 346 |
| HpyF3I | CTNAG | 4 cut(s) | 9, 14, 123, 309 |
| HspAI | GCGC | 3 cut(s) | 97, 109, 179 |
| LmnI | GCTCC | 3 cut(s) | 106, 231, 351 |
| Lsp1109I | GCAGC | 1 cut(s) | 33 |
| LweI | GCATC | 1 cut(s) | 363 |
| MaeI | CTAG | 5 cut(s) | 68, 228, 282, 303, 330 |
| MaeIII | GTNAC | 1 cut(s) | 267 |
| MbiI | CCGCTC | 1 cut(s) | 288 |
| MboII | GAAGA | 2 cut(s) | 184, 197 |
| MhlI | GDGCHC | 1 cut(s) | 227 |
| MluCI | AATT | 1 cut(s) | 158 |
| MlyI | GAGTC | 1 cut(s) | 275 |
| MnlI | CCTC | 6 cut(s) | 53, 86, 118, 253, 304, 329 |
| MslI | CAYNNNNRTG | 1 cut(s) | 149 |
| MspA1I | CMGCKG | 1 cut(s) | 349 |
| MspI | CCGG | 1 cut(s) | 249 |
| Mva1269I | GAATGC | 1 cut(s) | 196 |
| MvnI | CGCG | 1 cut(s) | 99 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 24, 200, 231, 346 |
| NlaIV | GGNNCC | 1 cut(s) | 366 |
| NmuCI | GTSAC | 1 cut(s) | 267 |
| PctI | GAATGC | 1 cut(s) | 196 |
| PinAI | ACCGGT | 1 cut(s) | 248 |
| PkrI | GCNGC | 6 cut(s) | 20, 23, 26, 49, 276, 287 |
| PleI | GAGTC | 1 cut(s) | 274 |
| PpsI | GAGTC | 1 cut(s) | 274 |
| PpuMI | RGGWCCY | 1 cut(s) | 40 |
| Psp5II | RGGWCCY | 1 cut(s) | 40 |
| PspFI | CCCAGC | 1 cut(s) | 199 |
| PspN4I | GGNNCC | 1 cut(s) | 366 |
| PspPI | GGNCC | 4 cut(s) | 40, 73, 127, 365 |
| PspPPI | RGGWCCY | 1 cut(s) | 40 |
| RseI | CAYNNNNRTG | 1 cut(s) | 149 |
| SatI | GCNGC | 6 cut(s) | 19, 22, 25, 48, 275, 286 |
| Sau96I | GGNCC | 4 cut(s) | 40, 73, 127, 365 |
| SchI | GAGTC | 1 cut(s) | 275 |
| SduI | GDGCHC | 1 cut(s) | 227 |
| SetI | ASST | 8 cut(s) | 10, 15, 45, 78, 141, 171, 257, 325 |
| SfaNI | GCATC | 1 cut(s) | 363 |
| SinI | GGWCC | 3 cut(s) | 40, 73, 365 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 149 |
| Sse9I | AATT | 1 cut(s) | 158 |
| SsiI | CCGC | 8 cut(s) | 18, 25, 48, 71, 263, 275, 286, 349 |
| SspMI | CTAG | 5 cut(s) | 68, 228, 282, 303, 330 |
| StyI | CCWWGG | 1 cut(s) | 329 |
| TaaI | ACNGT | 1 cut(s) | 216 |
| TaqI | TCGA | 1 cut(s) | 290 |
| TasI | AATT | 1 cut(s) | 158 |
| TauI | GCSGC | 5 cut(s) | 21, 27, 50, 277, 288 |
| TscAI | CASTG | 1 cut(s) | 227 |
| TseFI | GTSAC | 1 cut(s) | 267 |
| TseI | GCWGC | 1 cut(s) | 21 |
| Tsp45I | GTSAC | 1 cut(s) | 267 |
| TspRI | CASTG | 1 cut(s) | 227 |
| VneI | GTGCAC | 1 cut(s) | 223 |
| VpaK11BI | GGWCC | 3 cut(s) | 40, 73, 365 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 333 |
| XapI | RAATTY | 1 cut(s) | 158 |
| XmaJI | CCTAGG | 1 cut(s) | 329 |
| XspI | CTAG | 5 cut(s) | 68, 228, 282, 303, 330 |