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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 394 |
| Acc65I | GGTACC | 1 cut(s) | 406 |
| AccB1I | GGYRCC | 1 cut(s) | 406 |
| AccBSI | CCGCTC | 1 cut(s) | 170 |
| AccII | CGCG | 2 cut(s) | 90, 493 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfaI | GTAC | 4 cut(s) | 176, 381, 396, 408 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 202, 368, 545 |
| AjnI | CCWGG | 1 cut(s) | 544 |
| AluBI | AGCT | 2 cut(s) | 181, 512 |
| AluI | AGCT | 2 cut(s) | 181, 512 |
| AoxI | GGCC | 4 cut(s) | 154, 240, 246, 359 |
| Asp718I | GGTACC | 1 cut(s) | 406 |
| AspLEI | GCGC | 5 cut(s) | 92, 231, 325, 495, 552 |
| AspS9I | GGNCC | 6 cut(s) | 40, 154, 247, 360, 386, 440 |
| AsuC2I | CCSGG | 2 cut(s) | 117, 153 |
| AsuHPI | GGTGA | 3 cut(s) | 134, 137, 157 |
| AvaII | GGWCC | 3 cut(s) | 40, 386, 440 |
| BanI | GGYRCC | 1 cut(s) | 406 |
| BciT130I | CCWGG | 1 cut(s) | 546 |
| BcnI | CCSGG | 2 cut(s) | 117, 153 |
| BfaI | CTAG | 3 cut(s) | 107, 513, 574 |
| BfuAI | ACCTGC | 1 cut(s) | 394 |
| Bme1390I | CCNGG | 3 cut(s) | 117, 153, 546 |
| Bme18I | GGWCC | 3 cut(s) | 40, 386, 440 |
| BmgT120I | GGNCC | 6 cut(s) | 40, 154, 247, 360, 386, 440 |
| BmiI | GGNNCC | 3 cut(s) | 387, 408, 441 |
| BmrFI | CCNGG | 3 cut(s) | 117, 153, 546 |
| BmrI | ACTGGG | 1 cut(s) | 392 |
| BmsI | GCATC | 3 cut(s) | 115, 295, 390 |
| BmuI | ACTGGG | 1 cut(s) | 392 |
| BpuMI | CCSGG | 2 cut(s) | 117, 153 |
| BsaBI | GATNNNNATC | 1 cut(s) | 129 |
| BsaJI | CCNNGG | 4 cut(s) | 115, 243, 544, 545 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 467, 497 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 202, 368, 545 |
| Bse118I | RCCGGY | 1 cut(s) | 357 |
| Bse1I | ACTGG | 5 cut(s) | 43, 192, 306, 373, 387 |
| Bse8I | GATNNNNATC | 1 cut(s) | 129 |
| BseBI | CCWGG | 1 cut(s) | 546 |
| BseDI | CCNNGG | 4 cut(s) | 115, 243, 544, 545 |
| BseGI | GGATG | 1 cut(s) | 310 |
| BseJI | GATNNNNATC | 1 cut(s) | 129 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 202, 368, 545 |
| BseMII | CTCAG | 2 cut(s) | 471, 516 |
| BseNI | ACTGG | 5 cut(s) | 43, 192, 306, 373, 387 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseYI | CCCAGC | 2 cut(s) | 70, 319 |
| Bsh1236I | CGCG | 2 cut(s) | 90, 493 |
| BshFI | GGCC | 4 cut(s) | 156, 242, 248, 361 |
| BshNI | GGYRCC | 1 cut(s) | 406 |
| BsiSI | CCGG | 3 cut(s) | 117, 152, 358 |
| BslFI | GGGAC | 3 cut(s) | 399, 437, 453 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 202, 368, 545 |
| BsmFI | GGGAC | 3 cut(s) | 399, 437, 453 |
| BsnI | GGCC | 4 cut(s) | 156, 242, 248, 361 |
| BspANI | GGCC | 4 cut(s) | 156, 242, 248, 361 |
| BspCNI | CTCAG | 2 cut(s) | 472, 517 |
| BspFNI | CGCG | 2 cut(s) | 90, 493 |
| BspLI | GGNNCC | 3 cut(s) | 387, 408, 441 |
| BspMI | ACCTGC | 1 cut(s) | 394 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 406 |
| BsrBI | CCGCTC | 1 cut(s) | 170 |
| BsrFI | RCCGGY | 1 cut(s) | 357 |
| BsrI | ACTGG | 5 cut(s) | 43, 192, 306, 373, 387 |
| BssAI | RCCGGY | 1 cut(s) | 357 |
| BssECI | CCNNGG | 4 cut(s) | 115, 243, 544, 545 |
| Bst2UI | CCWGG | 1 cut(s) | 546 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 501 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 183, 332, 359, 540 |
| BstDEI | CTNAG | 2 cut(s) | 480, 525 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 200, 543 |
| BstF5I | GGATG | 1 cut(s) | 310 |
| BstFNI | CGCG | 2 cut(s) | 90, 493 |
| BstHHI | GCGC | 5 cut(s) | 92, 231, 325, 495, 552 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 87, 320, 331, 358, 501 |
| BstNI | CCWGG | 1 cut(s) | 546 |
| BstSCI | CCNGG | 3 cut(s) | 115, 151, 544 |
| BstUI | CGCG | 2 cut(s) | 90, 493 |
| BsuRI | GGCC | 4 cut(s) | 156, 242, 248, 361 |
| BtsCI | GGATG | 1 cut(s) | 310 |
| BtsIMutI | CAGTG | 4 cut(s) | 185, 299, 366, 410 |
| BveI | ACCTGC | 1 cut(s) | 394 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 183, 332, 359, 540 |
| CfoI | GCGC | 5 cut(s) | 92, 231, 325, 495, 552 |
| Cfr10I | RCCGGY | 1 cut(s) | 357 |
| Cfr13I | GGNCC | 6 cut(s) | 40, 154, 247, 360, 386, 440 |
| Csp6I | GTAC | 4 cut(s) | 175, 380, 395, 407 |
| CviQI | GTAC | 4 cut(s) | 175, 380, 395, 407 |
| DdeI | CTNAG | 2 cut(s) | 480, 525 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 3 cut(s) | 37, 53, 525 |
| Eco47I | GGWCC | 3 cut(s) | 40, 386, 440 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 200, 543 |
| EcoRII | CCWGG | 1 cut(s) | 544 |
| FaqI | GGGAC | 3 cut(s) | 399, 437, 453 |
| FokI | GGATG | 1 cut(s) | 317 |
| FspBI | CTAG | 3 cut(s) | 107, 513, 574 |
| GlaI | GCGC | 5 cut(s) | 91, 230, 324, 494, 551 |
| GsaI | CCCAGC | 2 cut(s) | 74, 323 |
| HaeIII | GGCC | 4 cut(s) | 156, 242, 248, 361 |
| HapII | CCGG | 3 cut(s) | 117, 152, 358 |
| HhaI | GCGC | 5 cut(s) | 92, 231, 325, 495, 552 |
| Hin6I | GCGC | 5 cut(s) | 90, 229, 323, 493, 550 |
| HinP1I | GCGC | 5 cut(s) | 90, 229, 323, 493, 550 |
| HinfI | GANTC | 1 cut(s) | 482 |
| HpaII | CCGG | 3 cut(s) | 117, 152, 358 |
| HphI | GGTGA | 3 cut(s) | 134, 137, 157 |
| Hpy188I | TCNGA | 1 cut(s) | 508 |
| Hpy188III | TCNNGA | 1 cut(s) | 339 |
| HpyAV | CCTTC | 2 cut(s) | 206, 232 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 2 cut(s) | 277, 374 |
| HpyCH4V | TGCA | 3 cut(s) | 128, 308, 403 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 87, 320, 331, 358, 501 |
| HpyF3I | CTNAG | 2 cut(s) | 480, 525 |
| HpySE526I | ACGT | 2 cut(s) | 277, 374 |
| HspAI | GCGC | 5 cut(s) | 90, 229, 323, 493, 550 |
| KpnI | GGTACC | 1 cut(s) | 410 |
| KroI | GCCGGC | 1 cut(s) | 357 |
| KroNI | GCCGGC | 1 cut(s) | 359 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 2 cut(s) | 226, 469 |
| LweI | GCATC | 3 cut(s) | 115, 295, 390 |
| MaeI | CTAG | 3 cut(s) | 107, 513, 574 |
| MaeII | ACGT | 2 cut(s) | 277, 374 |
| MaeIII | GTNAC | 3 cut(s) | 143, 273, 483 |
| MbiI | CCGCTC | 1 cut(s) | 170 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MlyI | GAGTC | 1 cut(s) | 491 |
| MmeI | TCCRAC | 1 cut(s) | 447 |
| MnlI | CCTC | 8 cut(s) | 214, 217, 238, 288, 429, 465, 469, 520 |
| MroNI | GCCGGC | 1 cut(s) | 357 |
| MseI | TTAA | 1 cut(s) | 84 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 565 |
| MspI | CCGG | 3 cut(s) | 117, 152, 358 |
| MspR9I | CCNGG | 3 cut(s) | 117, 153, 546 |
| MvaI | CCWGG | 1 cut(s) | 546 |
| MvnI | CGCG | 2 cut(s) | 90, 493 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 87, 320, 331, 358, 501 |
| NaeI | GCCGGC | 1 cut(s) | 359 |
| NciI | CCSGG | 2 cut(s) | 117, 153 |
| NgoMIV | GCCGGC | 1 cut(s) | 357 |
| NlaIV | GGNNCC | 3 cut(s) | 387, 408, 441 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 3 cut(s) | 143, 273, 483 |
| PasI | CCCWGGG | 1 cut(s) | 545 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PdiI | GCCGGC | 1 cut(s) | 359 |
| PleI | GAGTC | 1 cut(s) | 490 |
| PpsI | GAGTC | 1 cut(s) | 490 |
| Psp6I | CCWGG | 1 cut(s) | 544 |
| PspFI | CCCAGC | 2 cut(s) | 70, 319 |
| PspGI | CCWGG | 1 cut(s) | 544 |
| PspN4I | GGNNCC | 3 cut(s) | 387, 408, 441 |
| PspPI | GGNCC | 6 cut(s) | 40, 154, 247, 360, 386, 440 |
| RsaI | GTAC | 4 cut(s) | 176, 381, 396, 408 |
| RsaNI | GTAC | 4 cut(s) | 175, 380, 395, 407 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| Sau96I | GGNCC | 6 cut(s) | 40, 154, 247, 360, 386, 440 |
| SchI | GAGTC | 1 cut(s) | 491 |
| ScrFI | CCNGG | 3 cut(s) | 117, 153, 546 |
| SetI | ASST | 8 cut(s) | 183, 198, 280, 377, 408, 424, 514, 561 |
| SfaNI | GCATC | 3 cut(s) | 115, 295, 390 |
| SinI | GGWCC | 3 cut(s) | 40, 386, 440 |
| SspMI | CTAG | 3 cut(s) | 107, 513, 574 |
| StyD4I | CCNGG | 3 cut(s) | 115, 151, 544 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 2 cut(s) | 280, 377 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TscAI | CASTG | 4 cut(s) | 192, 306, 373, 417 |
| TseFI | GTSAC | 3 cut(s) | 143, 273, 483 |
| Tsp45I | GTSAC | 3 cut(s) | 143, 273, 483 |
| TspDTI | ATGAA | 1 cut(s) | 300 |
| TspGWI | ACGGA | 1 cut(s) | 225 |
| TspRI | CASTG | 4 cut(s) | 192, 306, 373, 417 |
| VpaK11BI | GGWCC | 3 cut(s) | 40, 386, 440 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 200, 543 |
| XspI | CTAG | 3 cut(s) | 107, 513, 574 |