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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1384 |
| AccB1I | GGYRCC | 1 cut(s) | 369 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1099 |
| AccI | GTMKAC | 2 cut(s) | 847, 1399 |
| AccII | CGCG | 7 cut(s) | 355, 365, 367, 563, 708, 1208, 1532 |
| AclWI | GGATC | 7 cut(s) | 338, 349, 657, 690, 1016, 1126, 1271 |
| AcoI | YGGCCR | 2 cut(s) | 597, 795 |
| AcsI | RAATTY | 1 cut(s) | 1471 |
| AcuI | CTGAAG | 1 cut(s) | 537 |
| AcyI | GRCGYC | 3 cut(s) | 1149, 1335, 1381 |
| AdeI | CACNNNGTG | 1 cut(s) | 1344 |
| AfaI | GTAC | 3 cut(s) | 298, 665, 897 |
| AflII | CTTAAG | 1 cut(s) | 137 |
| AflIII | ACRYGT | 2 cut(s) | 5, 764 |
| AgsI | TTSAA | 5 cut(s) | 227, 262, 997, 1303, 1470 |
| AjiI | CACGTC | 1 cut(s) | 8 |
| AjnI | CCWGG | 6 cut(s) | 304, 622, 645, 836, 871, 910 |
| AleI | CACNNNNGTG | 1 cut(s) | 58 |
| AluBI | AGCT | 8 cut(s) | 50, 97, 268, 390, 399, 993, 1408, 1446 |
| AluI | AGCT | 8 cut(s) | 50, 97, 268, 390, 399, 993, 1408, 1446 |
| Alw21I | GWGCWC | 2 cut(s) | 583, 1368 |
| Alw26I | GTCTC | 5 cut(s) | 454, 1136, 1388, 1422, 1435 |
| Alw44I | GTGCAC | 2 cut(s) | 579, 1364 |
| AlwI | GGATC | 7 cut(s) | 338, 349, 657, 690, 1016, 1126, 1271 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 452, 1013 |
| AoxI | GGCC | 8 cut(s) | 311, 597, 795, 875, 1065, 1192, 1218, 1226 |
| ApaLI | GTGCAC | 2 cut(s) | 579, 1364 |
| ApeKI | GCWGC | 6 cut(s) | 265, 627, 630, 752, 831, 866 |
| ApoI | RAATTY | 1 cut(s) | 1471 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 49, 975 |
| AspA2I | CCTAGG | 1 cut(s) | 1034 |
| AspLEI | GCGC | 7 cut(s) | 357, 367, 757, 857, 871, 1041, 1532 |
| AspS9I | GGNCC | 4 cut(s) | 311, 875, 929, 1193 |
| AsuC2I | CCSGG | 6 cut(s) | 339, 596, 606, 809, 1136, 1191 |
| AsuHPI | GGTGA | 9 cut(s) | 104, 325, 336, 450, 478, 649, 1031, 1303, 1382 |
| AvaII | GGWCC | 1 cut(s) | 929 |
| AvrII | CCTAGG | 1 cut(s) | 1034 |
| BaeGI | GKGCMC | 2 cut(s) | 583, 1368 |
| BanI | GGYRCC | 1 cut(s) | 369 |
| BbsI | GAAGAC | 1 cut(s) | 1457 |
| Bbv12I | GWGCWC | 2 cut(s) | 583, 1368 |
| BbvCI | CCTCAGC | 1 cut(s) | 321 |
| BbvI | GCAGC | 6 cut(s) | 277, 639, 642, 739, 843, 878 |
| BccI | CCATC | 8 cut(s) | 12, 83, 122, 182, 236, 548, 664, 1356 |
| BceAI | ACGGC | 1 cut(s) | 1205 |
| BciT130I | CCWGG | 6 cut(s) | 306, 624, 647, 838, 873, 912 |
| BcnI | CCSGG | 6 cut(s) | 339, 596, 606, 809, 1136, 1191 |
| BcoDI | GTCTC | 5 cut(s) | 454, 1136, 1388, 1422, 1435 |
| BfaI | CTAG | 4 cut(s) | 42, 119, 1035, 1461 |
| BfmI | CTRYAG | 1 cut(s) | 1214 |
| BfoI | RGCGCY | 2 cut(s) | 858, 872 |
| BfrI | CTTAAG | 1 cut(s) | 137 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 310, 378, 1051 |
| BlnI | CCTAGG | 1 cut(s) | 1034 |
| Bme18I | GGWCC | 1 cut(s) | 929 |
| BmgBI | CACGTC | 1 cut(s) | 8 |
| BmgT120I | GGNCC | 4 cut(s) | 311, 875, 929, 1193 |
| BmiI | GGNNCC | 5 cut(s) | 371, 930, 1169, 1195, 1503 |
| BmrI | ACTGGG | 1 cut(s) | 1087 |
| BmsI | GCATC | 4 cut(s) | 217, 390, 1098, 1533 |
| BmuI | ACTGGG | 1 cut(s) | 1087 |
| BoxI | GACNNNNGTC | 2 cut(s) | 1148, 1358 |
| BpiI | GAAGAC | 1 cut(s) | 1457 |
| BpmI | CTGGAG | 2 cut(s) | 131, 1139 |
| Bpu10I | CCTNAGC | 1 cut(s) | 321 |
| BpuMI | CCSGG | 6 cut(s) | 339, 596, 606, 809, 1136, 1191 |
| BsaAI | YACGTR | 1 cut(s) | 895 |
| BsaHI | GRCGYC | 3 cut(s) | 1149, 1335, 1381 |
| BsaI | GGTCTC | 1 cut(s) | 1435 |
| BsaJI | CCNNGG | 7 cut(s) | 337, 594, 604, 872, 911, 1034, 1497 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1112, 1142 |
| Bse118I | RCCGGY | 3 cut(s) | 857, 877, 1076 |
| Bse1I | ACTGG | 4 cut(s) | 234, 681, 1093, 1122 |
| BseBI | CCWGG | 6 cut(s) | 306, 624, 647, 838, 873, 912 |
| BseDI | CCNNGG | 7 cut(s) | 337, 594, 604, 872, 911, 1034, 1497 |
| BseGI | GGATG | 5 cut(s) | 23, 193, 472, 540, 1113 |
| BseMII | CTCAG | 2 cut(s) | 335, 1005 |
| BseNI | ACTGG | 4 cut(s) | 234, 681, 1093, 1122 |
| BseRI | GAGGAG | 1 cut(s) | 252 |
| BseSI | GKGCMC | 2 cut(s) | 583, 1368 |
| BseXI | GCAGC | 6 cut(s) | 277, 639, 642, 739, 843, 878 |
| BseYI | CCCAGC | 2 cut(s) | 399, 851 |
| Bsh1236I | CGCG | 7 cut(s) | 355, 365, 367, 563, 708, 1208, 1532 |
| Bsh1285I | CGRYCG | 2 cut(s) | 567, 805 |
| BshFI | GGCC | 8 cut(s) | 313, 599, 797, 877, 1067, 1194, 1220, 1228 |
| BshNI | GGYRCC | 1 cut(s) | 369 |
| BsiEI | CGRYCG | 2 cut(s) | 567, 805 |
| BsiHKAI | GWGCWC | 2 cut(s) | 583, 1368 |
| BsiSI | CCGG | 9 cut(s) | 339, 596, 606, 809, 858, 878, 1077, 1135, 1191 |
| BslFI | GGGAC | 3 cut(s) | 405, 942, 1024 |
| BsmAI | GTCTC | 5 cut(s) | 454, 1136, 1388, 1422, 1435 |
| BsmBI | CGTCTC | 2 cut(s) | 1136, 1388 |
| BsmFI | GGGAC | 3 cut(s) | 405, 942, 1024 |
| BsmI | GAATGC | 1 cut(s) | 894 |
| BsnI | GGCC | 8 cut(s) | 313, 599, 797, 877, 1067, 1194, 1220, 1228 |
| Bso31I | GGTCTC | 1 cut(s) | 1435 |
| Bsp1286I | GDGCHC | 2 cut(s) | 583, 1368 |
| BspANI | GGCC | 8 cut(s) | 313, 599, 797, 877, 1067, 1194, 1220, 1228 |
| BspCNI | CTCAG | 2 cut(s) | 334, 1006 |
| BspFNI | CGCG | 7 cut(s) | 355, 365, 367, 563, 708, 1208, 1532 |
| BspHI | TCATGA | 1 cut(s) | 759 |
| BspLI | GGNNCC | 5 cut(s) | 371, 930, 1169, 1195, 1503 |
| BspPI | GGATC | 7 cut(s) | 338, 349, 657, 690, 1016, 1126, 1271 |
| BspQI | GCTCTTC | 2 cut(s) | 40, 513 |
| BspT107I | GGYRCC | 1 cut(s) | 369 |
| BspTI | CTTAAG | 1 cut(s) | 137 |
| BspTNI | GGTCTC | 1 cut(s) | 1435 |
| BsrFI | RCCGGY | 3 cut(s) | 857, 877, 1076 |
| BsrI | ACTGG | 4 cut(s) | 234, 681, 1093, 1122 |
| BssAI | RCCGGY | 3 cut(s) | 857, 877, 1076 |
| BssECI | CCNNGG | 7 cut(s) | 337, 594, 604, 872, 911, 1034, 1497 |
| BssNAI | GTATAC | 1 cut(s) | 1400 |
| BssNI | GRCGYC | 3 cut(s) | 1149, 1335, 1381 |
| BssT1I | CCWWGG | 2 cut(s) | 1034, 1497 |
| Bst1107I | GTATAC | 1 cut(s) | 1400 |
| Bst2UI | CCWGG | 6 cut(s) | 306, 624, 647, 838, 873, 912 |
| Bst4CI | ACNGT | 7 cut(s) | 125, 146, 181, 578, 1357, 1370, 1397 |
| Bst6I | CTCTTC | 2 cut(s) | 40, 513 |
| BstACI | GRCGYC | 3 cut(s) | 1149, 1335, 1381 |
| BstAFI | CTTAAG | 1 cut(s) | 137 |
| BstBAI | YACGTR | 1 cut(s) | 895 |
| BstC8I | GCNNGC | 7 cut(s) | 311, 638, 821, 859, 984, 1043, 1078 |
| BstDEI | CTNAG | 3 cut(s) | 79, 321, 1014 |
| BstEII | GGTNACC | 1 cut(s) | 1309 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 465 |
| BstF5I | GGATG | 5 cut(s) | 23, 193, 472, 540, 1113 |
| BstFNI | CGCG | 7 cut(s) | 355, 365, 367, 563, 708, 1208, 1532 |
| BstH2I | RGCGCY | 2 cut(s) | 858, 872 |
| BstHHI | GCGC | 7 cut(s) | 357, 367, 757, 857, 871, 1041, 1532 |
| BstMAI | GTCTC | 5 cut(s) | 454, 1136, 1388, 1422, 1435 |
| BstMCI | CGRYCG | 2 cut(s) | 567, 805 |
| BstNI | CCWGG | 6 cut(s) | 306, 624, 647, 838, 873, 912 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 1148, 1358 |
| BstPI | GGTNACC | 1 cut(s) | 1309 |
| BstSFI | CTRYAG | 1 cut(s) | 1214 |
| BstSLI | GKGCMC | 2 cut(s) | 583, 1368 |
| BstUI | CGCG | 7 cut(s) | 355, 365, 367, 563, 708, 1208, 1532 |
| BstV1I | GCAGC | 6 cut(s) | 277, 639, 642, 739, 843, 878 |
| BstV2I | GAAGAC | 1 cut(s) | 1457 |
| BstX2I | RGATCY | 2 cut(s) | 649, 682 |
| BstYI | RGATCY | 2 cut(s) | 649, 682 |
| BstZ17I | GTATAC | 1 cut(s) | 1400 |
| BsuRI | GGCC | 8 cut(s) | 313, 599, 797, 877, 1067, 1194, 1220, 1228 |
| BtrI | CACGTC | 1 cut(s) | 8 |
| BtsCI | GGATG | 5 cut(s) | 23, 193, 472, 540, 1113 |
| BtsIMutI | CAGTG | 2 cut(s) | 130, 674 |
| Cac8I | GCNNGC | 7 cut(s) | 311, 638, 821, 859, 984, 1043, 1078 |
| CaiI | CAGNNNCTG | 2 cut(s) | 452, 1013 |
| CciI | TCATGA | 1 cut(s) | 759 |
| CfoI | GCGC | 7 cut(s) | 357, 367, 757, 857, 871, 1041, 1532 |
| Cfr10I | RCCGGY | 3 cut(s) | 857, 877, 1076 |
| Cfr13I | GGNCC | 4 cut(s) | 311, 875, 929, 1193 |
| CseI | GACGC | 5 cut(s) | 314, 361, 1138, 1343, 1521 |
| Csp6I | GTAC | 3 cut(s) | 297, 664, 896 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 897, 932 |
| CviAII | CATG | 4 cut(s) | 490, 661, 760, 1291 |
| CviQI | GTAC | 3 cut(s) | 297, 664, 896 |
| DdeI | CTNAG | 3 cut(s) | 79, 321, 1014 |
| DraIII | CACNNNGTG | 1 cut(s) | 1344 |
| EaeI | YGGCCR | 2 cut(s) | 597, 795 |
| Eam1104I | CTCTTC | 2 cut(s) | 40, 513 |
| EarI | CTCTTC | 2 cut(s) | 40, 513 |
| EciI | GGCGGA | 8 cut(s) | 174, 624, 695, 1014, 1063, 1154, 1192, 1281 |
| Eco130I | CCWWGG | 2 cut(s) | 1034, 1497 |
| Eco31I | GGTCTC | 1 cut(s) | 1435 |
| Eco47I | GGWCC | 1 cut(s) | 929 |
| Eco57I | CTGAAG | 1 cut(s) | 537 |
| Eco91I | GGTNACC | 1 cut(s) | 1309 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 465 |
| EcoO65I | GGTNACC | 1 cut(s) | 1309 |
| EcoRII | CCWGG | 6 cut(s) | 304, 622, 645, 836, 871, 910 |
| EcoT14I | CCWWGG | 2 cut(s) | 1034, 1497 |
| ErhI | CCWWGG | 2 cut(s) | 1034, 1497 |
| Esp3I | CGTCTC | 2 cut(s) | 1136, 1388 |
| FaeI | CATG | 4 cut(s) | 493, 664, 763, 1294 |
| FaqI | GGGAC | 3 cut(s) | 405, 942, 1024 |
| FatI | CATG | 4 cut(s) | 489, 660, 759, 1290 |
| FauI | CCCGC | 7 cut(s) | 302, 497, 544, 568, 645, 1047, 1179 |
| FauNDI | CATATG | 2 cut(s) | 1234, 1261 |
| FblI | GTMKAC | 2 cut(s) | 847, 1399 |
| FokI | GGATG | 5 cut(s) | 30, 200, 459, 527, 1120 |
| FspBI | CTAG | 4 cut(s) | 42, 119, 1035, 1461 |
| GlaI | GCGC | 7 cut(s) | 356, 366, 756, 856, 870, 1040, 1531 |
| GsaI | CCCAGC | 2 cut(s) | 403, 855 |
| GsuI | CTGGAG | 2 cut(s) | 131, 1139 |
| HaeII | RGCGCY | 2 cut(s) | 858, 872 |
| HaeIII | GGCC | 8 cut(s) | 313, 599, 797, 877, 1067, 1194, 1220, 1228 |
| HapII | CCGG | 9 cut(s) | 339, 596, 606, 809, 858, 878, 1077, 1135, 1191 |
| HgaI | GACGC | 5 cut(s) | 314, 361, 1138, 1343, 1521 |
| HhaI | GCGC | 7 cut(s) | 357, 367, 757, 857, 871, 1041, 1532 |
| Hin1I | GRCGYC | 3 cut(s) | 1149, 1335, 1381 |
| Hin1II | CATG | 4 cut(s) | 493, 664, 763, 1294 |
| Hin6I | GCGC | 7 cut(s) | 355, 365, 755, 855, 869, 1039, 1530 |
| HinP1I | GCGC | 7 cut(s) | 355, 365, 755, 855, 869, 1039, 1530 |
| HincII | GTYRAC | 2 cut(s) | 574, 848 |
| HindII | GTYRAC | 2 cut(s) | 574, 848 |
| HindIII | AAGCTT | 1 cut(s) | 1406 |
| HinfI | GANTC | 5 cut(s) | 115, 971, 1060, 1070, 1326 |
| HpaI | GTTAAC | 1 cut(s) | 574 |
| HpaII | CCGG | 9 cut(s) | 339, 596, 606, 809, 858, 878, 1077, 1135, 1191 |
| HphI | GGTGA | 9 cut(s) | 104, 325, 336, 450, 478, 649, 1031, 1303, 1382 |
| Hpy166II | GTNNAC | 9 cut(s) | 252, 299, 574, 581, 848, 1179, 1288, 1366, 1400 |
| Hpy188I | TCNGA | 6 cut(s) | 330, 556, 733, 1167, 1353, 1435 |
| Hpy188III | TCNNGA | 9 cut(s) | 119, 224, 289, 589, 760, 921, 1153, 1185, 1303 |
| Hpy8I | GTNNAC | 9 cut(s) | 252, 299, 574, 581, 848, 1179, 1288, 1366, 1400 |
| Hpy99I | CGWCG | 7 cut(s) | 185, 330, 355, 1334, 1337, 1383, 1459 |
| HpyAV | CCTTC | 3 cut(s) | 986, 1300, 1309 |
| HpyCH4III | ACNGT | 7 cut(s) | 125, 146, 181, 578, 1357, 1370, 1397 |
| HpyCH4IV | ACGT | 4 cut(s) | 7, 766, 894, 1381 |
| HpyCH4V | TGCA | 5 cut(s) | 581, 892, 1111, 1238, 1366 |
| HpyF3I | CTNAG | 3 cut(s) | 79, 321, 1014 |
| HpySE526I | ACGT | 4 cut(s) | 7, 766, 894, 1381 |
| Hsp92I | GRCGYC | 3 cut(s) | 1149, 1335, 1381 |
| Hsp92II | CATG | 4 cut(s) | 493, 664, 763, 1294 |
| HspAI | GCGC | 7 cut(s) | 355, 365, 755, 855, 869, 1039, 1530 |
| KroI | GCCGGC | 2 cut(s) | 857, 1076 |
| KroNI | GCCGGC | 2 cut(s) | 859, 1078 |
| KspAI | GTTAAC | 1 cut(s) | 574 |
| LguI | GCTCTTC | 2 cut(s) | 40, 513 |
| LmnI | GCTCC | 2 cut(s) | 395, 1120 |
| Lsp1109I | GCAGC | 6 cut(s) | 277, 639, 642, 739, 843, 878 |
| LweI | GCATC | 4 cut(s) | 217, 390, 1098, 1533 |
| MaeI | CTAG | 4 cut(s) | 42, 119, 1035, 1461 |
| MaeII | ACGT | 4 cut(s) | 7, 766, 894, 1381 |
| MaeIII | GTNAC | 7 cut(s) | 72, 478, 655, 825, 1309, 1370, 1484 |
| MboII | GAAGA | 4 cut(s) | 57, 530, 1416, 1462 |
| MflI | RGATCY | 2 cut(s) | 649, 682 |
| MhlI | GDGCHC | 2 cut(s) | 583, 1368 |
| MluCI | AATT | 2 cut(s) | 27, 1471 |
| MlyI | GAGTC | 1 cut(s) | 1069 |
| MmeI | TCCRAC | 2 cut(s) | 308, 579 |
| MreI | CGCCGGCG | 1 cut(s) | 1076 |
| MroNI | GCCGGC | 2 cut(s) | 857, 1076 |
| MroXI | GAANNNNTTC | 2 cut(s) | 49, 975 |
| MseI | TTAA | 2 cut(s) | 138, 573 |
| MslI | CAYNNNNRTG | 2 cut(s) | 58, 887 |
| MspA1I | CMGCKG | 3 cut(s) | 504, 901, 1174 |
| MspCI | CTTAAG | 1 cut(s) | 137 |
| MspI | CCGG | 9 cut(s) | 339, 596, 606, 809, 858, 878, 1077, 1135, 1191 |
| Mva1269I | GAATGC | 1 cut(s) | 894 |
| MvaI | CCWGG | 6 cut(s) | 306, 624, 647, 838, 873, 912 |
| MvnI | CGCG | 7 cut(s) | 355, 365, 367, 563, 708, 1208, 1532 |
| NaeI | GCCGGC | 2 cut(s) | 859, 1078 |
| NciI | CCSGG | 6 cut(s) | 339, 596, 606, 809, 1136, 1191 |
| NdeI | CATATG | 2 cut(s) | 1234, 1261 |
| NgoMIV | GCCGGC | 2 cut(s) | 857, 1076 |
| NlaIII | CATG | 4 cut(s) | 493, 664, 763, 1294 |
| NlaIV | GGNNCC | 5 cut(s) | 371, 930, 1169, 1195, 1503 |
| NmeAIII | GCCGAG | 1 cut(s) | 1030 |
| NmuCI | GTSAC | 6 cut(s) | 72, 655, 825, 1309, 1370, 1484 |
| OliI | CACNNNNGTG | 1 cut(s) | 58 |
| PagI | TCATGA | 1 cut(s) | 759 |
| PciSI | GCTCTTC | 2 cut(s) | 40, 513 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1454 |
| PctI | GAATGC | 1 cut(s) | 894 |
| PdiI | GCCGGC | 2 cut(s) | 859, 1078 |
| PdmI | GAANNNNTTC | 2 cut(s) | 49, 975 |
| PfeI | GAWTC | 4 cut(s) | 115, 971, 1070, 1326 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1099 |
| Ple19I | CGATCG | 1 cut(s) | 567 |
| PleI | GAGTC | 1 cut(s) | 1068 |
| PpsI | GAGTC | 1 cut(s) | 1068 |
| Ppu21I | YACGTR | 1 cut(s) | 895 |
| PshAI | GACNNNNGTC | 2 cut(s) | 1148, 1358 |
| Psp6I | CCWGG | 6 cut(s) | 304, 622, 645, 836, 871, 910 |
| PspEI | GGTNACC | 1 cut(s) | 1309 |
| PspFI | CCCAGC | 2 cut(s) | 399, 851 |
| PspGI | CCWGG | 6 cut(s) | 304, 622, 645, 836, 871, 910 |
| PspN4I | GGNNCC | 5 cut(s) | 371, 930, 1169, 1195, 1503 |
| PspPI | GGNCC | 4 cut(s) | 311, 875, 929, 1193 |
| PstNI | CAGNNNCTG | 2 cut(s) | 452, 1013 |
| PsuI | RGATCY | 2 cut(s) | 649, 682 |
| PvuI | CGATCG | 1 cut(s) | 567 |
| RsaI | GTAC | 3 cut(s) | 298, 665, 897 |
| RsaNI | GTAC | 3 cut(s) | 297, 664, 896 |
| RseI | CAYNNNNRTG | 2 cut(s) | 58, 887 |
| SalI | GTCGAC | 1 cut(s) | 846 |
| SapI | GCTCTTC | 2 cut(s) | 40, 513 |
| SaqAI | TTAA | 2 cut(s) | 138, 573 |
| Sau96I | GGNCC | 4 cut(s) | 311, 875, 929, 1193 |
| SchI | GAGTC | 1 cut(s) | 1069 |
| SduI | GDGCHC | 2 cut(s) | 583, 1368 |
| SfaNI | GCATC | 4 cut(s) | 217, 390, 1098, 1533 |
| SfcI | CTRYAG | 1 cut(s) | 1214 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1076 |
| SinI | GGWCC | 1 cut(s) | 929 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 58, 887 |
| SmlI | CTYRAG | 1 cut(s) | 137 |
| SmoI | CTYRAG | 1 cut(s) | 137 |
| Sse9I | AATT | 2 cut(s) | 27, 1471 |
| SspMI | CTAG | 4 cut(s) | 42, 119, 1035, 1461 |
| StyI | CCWWGG | 2 cut(s) | 1034, 1497 |
| TaaI | ACNGT | 7 cut(s) | 125, 146, 181, 578, 1357, 1370, 1397 |
| TaiI | ACGT | 4 cut(s) | 10, 769, 897, 1384 |
| TaqI | TCGA | 8 cut(s) | 13, 25, 183, 219, 428, 847, 1329, 1448 |
| TaqII | GACCGA | 2 cut(s) | 1289, 1417 |
| TasI | AATT | 2 cut(s) | 27, 1471 |
| TauI | GCSGC | 4 cut(s) | 360, 636, 797, 1208 |
| TfiI | GAWTC | 4 cut(s) | 115, 971, 1070, 1326 |
| Tru1I | TTAA | 2 cut(s) | 138, 573 |
| Tru9I | TTAA | 2 cut(s) | 138, 573 |
| TscAI | CASTG | 2 cut(s) | 130, 681 |
| TseFI | GTSAC | 6 cut(s) | 72, 655, 825, 1309, 1370, 1484 |
| TseI | GCWGC | 6 cut(s) | 265, 627, 630, 752, 831, 866 |
| Tsp45I | GTSAC | 6 cut(s) | 72, 655, 825, 1309, 1370, 1484 |
| TspGWI | ACGGA | 1 cut(s) | 249 |
| TspRI | CASTG | 2 cut(s) | 130, 681 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1099 |
| Vha464I | CTTAAG | 1 cut(s) | 137 |
| VneI | GTGCAC | 2 cut(s) | 579, 1364 |
| VpaK11BI | GGWCC | 1 cut(s) | 929 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 465 |
| XapI | RAATTY | 1 cut(s) | 1471 |
| XbaI | TCTAGA | 1 cut(s) | 118 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 667 |
| XmaJI | CCTAGG | 1 cut(s) | 1034 |
| XmiI | GTMKAC | 2 cut(s) | 847, 1399 |
| XmnI | GAANNNNTTC | 2 cut(s) | 49, 975 |
| XspI | CTAG | 4 cut(s) | 42, 119, 1035, 1461 |
| ZraI | GACGTC | 1 cut(s) | 1382 |