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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 61 |
| AccII | CGCG | 6 cut(s) | 66, 76, 83, 216, 374, 390 |
| AclWI | GGATC | 2 cut(s) | 468, 501 |
| AcoI | YGGCCR | 1 cut(s) | 408 |
| AcuI | CTGAAG | 2 cut(s) | 146, 348 |
| AcyI | GRCGYC | 1 cut(s) | 230 |
| AfaI | GTAC | 1 cut(s) | 476 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 19, 117, 185, 377 |
| AgsI | TTSAA | 1 cut(s) | 202 |
| AjnI | CCWGG | 3 cut(s) | 15, 433, 456 |
| AluBI | AGCT | 1 cut(s) | 108 |
| AluI | AGCT | 1 cut(s) | 108 |
| Alw21I | GWGCWC | 1 cut(s) | 345 |
| Alw26I | GTCTC | 2 cut(s) | 172, 224 |
| AlwI | GGATC | 2 cut(s) | 468, 501 |
| AoxI | GGCC | 2 cut(s) | 408, 454 |
| ApeKI | GCWGC | 2 cut(s) | 438, 441 |
| AspLEI | GCGC | 7 cut(s) | 68, 78, 85, 216, 392, 519, 531 |
| AspS9I | GGNCC | 1 cut(s) | 41 |
| AsuC2I | CCSGG | 2 cut(s) | 407, 417 |
| AsuHPI | GGTGA | 4 cut(s) | 168, 289, 434, 460 |
| AvaII | GGWCC | 1 cut(s) | 41 |
| BbsI | GAAGAC | 1 cut(s) | 209 |
| Bbv12I | GWGCWC | 1 cut(s) | 345 |
| BbvCI | CCTCAGC | 1 cut(s) | 32 |
| BbvI | GCAGC | 2 cut(s) | 450, 453 |
| BccI | CCATC | 1 cut(s) | 52 |
| BciT130I | CCWGG | 3 cut(s) | 17, 435, 458 |
| BciVI | GTATCC | 1 cut(s) | 17 |
| BcnI | CCSGG | 2 cut(s) | 407, 417 |
| BcoDI | GTCTC | 2 cut(s) | 172, 224 |
| BfuI | GTATCC | 1 cut(s) | 17 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 453 |
| BisI | GCNGC | 4 cut(s) | 69, 439, 442, 445 |
| BlsI | GCNGC | 4 cut(s) | 70, 440, 443, 446 |
| Bme1390I | CCNGG | 5 cut(s) | 17, 407, 417, 435, 458 |
| Bme18I | GGWCC | 1 cut(s) | 41 |
| BmgT120I | GGNCC | 1 cut(s) | 41 |
| BmrFI | CCNGG | 5 cut(s) | 17, 407, 417, 435, 458 |
| BmrI | ACTGGG | 1 cut(s) | 127 |
| BmsI | GCATC | 2 cut(s) | 108, 198 |
| BmuI | ACTGGG | 1 cut(s) | 127 |
| BpiI | GAAGAC | 1 cut(s) | 209 |
| BpmI | CTGGAG | 1 cut(s) | 33 |
| Bpu10I | CCTNAGC | 1 cut(s) | 32 |
| BpuMI | CCSGG | 2 cut(s) | 407, 417 |
| BsaHI | GRCGYC | 1 cut(s) | 230 |
| BsaI | GGTCTC | 1 cut(s) | 224 |
| BsaJI | CCNNGG | 2 cut(s) | 405, 550 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 19, 117, 185, 377 |
| Bse1I | ACTGG | 2 cut(s) | 122, 492 |
| BseBI | CCWGG | 3 cut(s) | 17, 435, 458 |
| BseDI | CCNNGG | 2 cut(s) | 405, 550 |
| BseGI | GGATG | 1 cut(s) | 283 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 19, 117, 185, 377 |
| BseMII | CTCAG | 1 cut(s) | 46 |
| BseNI | ACTGG | 2 cut(s) | 122, 492 |
| BseRI | GAGGAG | 1 cut(s) | 539 |
| BseXI | GCAGC | 2 cut(s) | 450, 453 |
| BseYI | CCCAGC | 1 cut(s) | 210 |
| Bsh1236I | CGCG | 6 cut(s) | 66, 76, 83, 216, 374, 390 |
| Bsh1285I | CGRYCG | 1 cut(s) | 378 |
| BshFI | GGCC | 2 cut(s) | 410, 456 |
| BsiEI | CGRYCG | 1 cut(s) | 378 |
| BsiHKAI | GWGCWC | 1 cut(s) | 345 |
| BsiSI | CCGG | 2 cut(s) | 407, 417 |
| BslFI | GGGAC | 1 cut(s) | 123 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 19, 117, 185, 377 |
| BsmAI | GTCTC | 2 cut(s) | 172, 224 |
| BsmFI | GGGAC | 1 cut(s) | 123 |
| BsnI | GGCC | 2 cut(s) | 410, 456 |
| Bso31I | GGTCTC | 1 cut(s) | 224 |
| Bsp1286I | GDGCHC | 1 cut(s) | 345 |
| Bsp143I | GATC | 4 cut(s) | 52, 375, 460, 493 |
| BspANI | GGCC | 2 cut(s) | 410, 456 |
| BspCNI | CTCAG | 1 cut(s) | 45 |
| BspFNI | CGCG | 6 cut(s) | 66, 76, 83, 216, 374, 390 |
| BspPI | GGATC | 2 cut(s) | 468, 501 |
| BspQI | GCTCTTC | 1 cut(s) | 324 |
| BspTNI | GGTCTC | 1 cut(s) | 224 |
| BsrI | ACTGG | 2 cut(s) | 122, 492 |
| BssECI | CCNNGG | 2 cut(s) | 405, 550 |
| BssMI | GATC | 4 cut(s) | 52, 375, 460, 493 |
| BssNI | GRCGYC | 1 cut(s) | 230 |
| BssT1I | CCWWGG | 1 cut(s) | 550 |
| Bst2UI | CCWGG | 3 cut(s) | 17, 435, 458 |
| Bst4CI | ACNGT | 2 cut(s) | 59, 311 |
| Bst6I | CTCTTC | 2 cut(s) | 175, 324 |
| BstACI | GRCGYC | 1 cut(s) | 230 |
| BstC8I | GCNNGC | 1 cut(s) | 336 |
| BstDEI | CTNAG | 1 cut(s) | 32 |
| BstF5I | GGATG | 1 cut(s) | 283 |
| BstFNI | CGCG | 6 cut(s) | 66, 76, 83, 216, 374, 390 |
| BstHHI | GCGC | 7 cut(s) | 68, 78, 85, 216, 392, 519, 531 |
| BstKTI | GATC | 4 cut(s) | 55, 378, 463, 496 |
| BstMAI | GTCTC | 2 cut(s) | 172, 224 |
| BstMBI | GATC | 4 cut(s) | 52, 375, 460, 493 |
| BstMCI | CGRYCG | 1 cut(s) | 378 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 82, 96, 105, 340, 387, 444, 453 |
| BstNI | CCWGG | 3 cut(s) | 17, 435, 458 |
| BstSCI | CCNGG | 5 cut(s) | 15, 405, 415, 433, 456 |
| BstUI | CGCG | 6 cut(s) | 66, 76, 83, 216, 374, 390 |
| BstV1I | GCAGC | 2 cut(s) | 450, 453 |
| BstV2I | GAAGAC | 1 cut(s) | 209 |
| BstX2I | RGATCY | 2 cut(s) | 460, 493 |
| BstYI | RGATCY | 2 cut(s) | 460, 493 |
| BsuI | GTATCC | 1 cut(s) | 17 |
| BsuRI | GGCC | 2 cut(s) | 410, 456 |
| BtsCI | GGATG | 1 cut(s) | 283 |
| BtsIMutI | CAGTG | 2 cut(s) | 55, 485 |
| Cac8I | GCNNGC | 1 cut(s) | 336 |
| CfoI | GCGC | 7 cut(s) | 68, 78, 85, 216, 392, 519, 531 |
| Cfr13I | GGNCC | 1 cut(s) | 41 |
| CseI | GACGC | 3 cut(s) | 25, 72, 238 |
| Csp6I | GTAC | 1 cut(s) | 475 |
| CviAII | CATG | 2 cut(s) | 301, 472 |
| CviJI | RGCY | 7 cut(s) | 108, 410, 428, 444, 456, 542, 548 |
| CviKI_1 | RGCY | 7 cut(s) | 108, 410, 428, 444, 456, 542, 548 |
| CviQI | GTAC | 1 cut(s) | 475 |
| DdeI | CTNAG | 1 cut(s) | 32 |
| DpnI | GATC | 4 cut(s) | 54, 377, 462, 495 |
| DpnII | GATC | 4 cut(s) | 52, 375, 460, 493 |
| EaeI | YGGCCR | 1 cut(s) | 408 |
| Eam1104I | CTCTTC | 2 cut(s) | 175, 324 |
| EarI | CTCTTC | 2 cut(s) | 175, 324 |
| EciI | GGCGGA | 2 cut(s) | 353, 506 |
| Eco130I | CCWWGG | 1 cut(s) | 550 |
| Eco31I | GGTCTC | 1 cut(s) | 224 |
| Eco47I | GGWCC | 1 cut(s) | 41 |
| Eco57I | CTGAAG | 2 cut(s) | 146, 348 |
| EcoRII | CCWGG | 3 cut(s) | 15, 433, 456 |
| EcoT14I | CCWWGG | 1 cut(s) | 550 |
| ErhI | CCWWGG | 1 cut(s) | 550 |
| FaeI | CATG | 2 cut(s) | 304, 475 |
| FaiI | YATR | 4 cut(s) | 132, 302, 473, 501 |
| FaqI | GGGAC | 1 cut(s) | 123 |
| FatI | CATG | 2 cut(s) | 300, 471 |
| FauI | CCCGC | 4 cut(s) | 13, 308, 355, 379 |
| FblI | GTMKAC | 1 cut(s) | 61 |
| Fnu4HI | GCNGC | 4 cut(s) | 69, 439, 442, 445 |
| FokI | GGATG | 1 cut(s) | 270 |
| Fsp4HI | GCNGC | 4 cut(s) | 69, 439, 442, 445 |
| GlaI | GCGC | 7 cut(s) | 67, 77, 84, 215, 391, 518, 530 |
| GluI | GCNGC | 4 cut(s) | 69, 439, 442, 445 |
| GsaI | CCCAGC | 1 cut(s) | 214 |
| GsuI | CTGGAG | 1 cut(s) | 33 |
| HaeIII | GGCC | 2 cut(s) | 410, 456 |
| HapII | CCGG | 2 cut(s) | 407, 417 |
| HgaI | GACGC | 3 cut(s) | 25, 72, 238 |
| HhaI | GCGC | 7 cut(s) | 68, 78, 85, 216, 392, 519, 531 |
| Hin1I | GRCGYC | 1 cut(s) | 230 |
| Hin1II | CATG | 2 cut(s) | 304, 475 |
| Hin6I | GCGC | 7 cut(s) | 66, 76, 83, 214, 390, 517, 529 |
| HinP1I | GCGC | 7 cut(s) | 66, 76, 83, 214, 390, 517, 529 |
| HincII | GTYRAC | 2 cut(s) | 62, 385 |
| HindII | GTYRAC | 2 cut(s) | 62, 385 |
| HpaI | GTTAAC | 1 cut(s) | 385 |
| HpaII | CCGG | 2 cut(s) | 407, 417 |
| HphI | GGTGA | 4 cut(s) | 168, 289, 434, 460 |
| Hpy166II | GTNNAC | 2 cut(s) | 62, 385 |
| Hpy188I | TCNGA | 5 cut(s) | 41, 104, 194, 228, 367 |
| Hpy188III | TCNNGA | 1 cut(s) | 50 |
| Hpy8I | GTNNAC | 2 cut(s) | 62, 385 |
| Hpy99I | CGWCG | 2 cut(s) | 41, 66 |
| HpyAV | CCTTC | 1 cut(s) | 532 |
| HpyCH4III | ACNGT | 2 cut(s) | 59, 311 |
| HpyCH4V | TGCA | 2 cut(s) | 4, 114 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 82, 96, 105, 340, 387, 444, 453 |
| HpyF3I | CTNAG | 1 cut(s) | 32 |
| Hsp92I | GRCGYC | 1 cut(s) | 230 |
| Hsp92II | CATG | 2 cut(s) | 304, 475 |
| HspAI | GCGC | 7 cut(s) | 66, 76, 83, 214, 390, 517, 529 |
| KspAI | GTTAAC | 1 cut(s) | 385 |
| Kzo9I | GATC | 4 cut(s) | 52, 375, 460, 493 |
| LguI | GCTCTTC | 1 cut(s) | 324 |
| LmnI | GCTCC | 3 cut(s) | 113, 340, 526 |
| Lsp1109I | GCAGC | 2 cut(s) | 450, 453 |
| LweI | GCATC | 2 cut(s) | 108, 198 |
| MaeIII | GTNAC | 1 cut(s) | 466 |
| MalI | GATC | 4 cut(s) | 54, 377, 462, 495 |
| MboI | GATC | 4 cut(s) | 52, 375, 460, 493 |
| MboII | GAAGA | 4 cut(s) | 162, 214, 341, 488 |
| MflI | RGATCY | 2 cut(s) | 460, 493 |
| MhlI | GDGCHC | 1 cut(s) | 345 |
| MmeI | TCCRAC | 2 cut(s) | 19, 390 |
| MnlI | CCTC | 6 cut(s) | 41, 184, 234, 373, 418, 517 |
| MseI | TTAA | 1 cut(s) | 384 |
| MspI | CCGG | 2 cut(s) | 407, 417 |
| MspR9I | CCNGG | 5 cut(s) | 17, 407, 417, 435, 458 |
| MvaI | CCWGG | 3 cut(s) | 17, 435, 458 |
| MvnI | CGCG | 6 cut(s) | 66, 76, 83, 216, 374, 390 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 82, 96, 105, 340, 387, 444, 453 |
| NciI | CCSGG | 2 cut(s) | 407, 417 |
| NdeII | GATC | 4 cut(s) | 52, 375, 460, 493 |
| NlaIII | CATG | 2 cut(s) | 304, 475 |
| NmuCI | GTSAC | 1 cut(s) | 466 |
| PciSI | GCTCTTC | 1 cut(s) | 324 |
| PkrI | GCNGC | 4 cut(s) | 70, 440, 443, 446 |
| Ple19I | CGATCG | 1 cut(s) | 378 |
| Psp6I | CCWGG | 3 cut(s) | 15, 433, 456 |
| PspFI | CCCAGC | 1 cut(s) | 210 |
| PspGI | CCWGG | 3 cut(s) | 15, 433, 456 |
| PspPI | GGNCC | 1 cut(s) | 41 |
| PsuI | RGATCY | 2 cut(s) | 460, 493 |
| PvuI | CGATCG | 1 cut(s) | 378 |
| RsaI | GTAC | 1 cut(s) | 476 |
| RsaNI | GTAC | 1 cut(s) | 475 |
| SalI | GTCGAC | 1 cut(s) | 60 |
| SapI | GCTCTTC | 1 cut(s) | 324 |
| SaqAI | TTAA | 1 cut(s) | 384 |
| SatI | GCNGC | 4 cut(s) | 69, 439, 442, 445 |
| Sau3AI | GATC | 4 cut(s) | 52, 375, 460, 493 |
| Sau96I | GGNCC | 1 cut(s) | 41 |
| ScrFI | CCNGG | 5 cut(s) | 17, 407, 417, 435, 458 |
| SduI | GDGCHC | 1 cut(s) | 345 |
| SetI | ASST | 3 cut(s) | 33, 110, 256 |
| SfaNI | GCATC | 2 cut(s) | 108, 198 |
| SinI | GGWCC | 1 cut(s) | 41 |
| StyD4I | CCNGG | 5 cut(s) | 15, 405, 415, 433, 456 |
| StyI | CCWWGG | 1 cut(s) | 550 |
| TaaI | ACNGT | 2 cut(s) | 59, 311 |
| TaqI | TCGA | 2 cut(s) | 61, 146 |
| TauI | GCSGC | 2 cut(s) | 71, 447 |
| Tru1I | TTAA | 1 cut(s) | 384 |
| Tru9I | TTAA | 1 cut(s) | 384 |
| TscAI | CASTG | 2 cut(s) | 62, 492 |
| TseFI | GTSAC | 1 cut(s) | 466 |
| TseI | GCWGC | 2 cut(s) | 438, 441 |
| Tsp45I | GTSAC | 1 cut(s) | 466 |
| TspDTI | ATGAA | 1 cut(s) | 488 |
| TspRI | CASTG | 2 cut(s) | 62, 492 |
| VpaK11BI | GGWCC | 1 cut(s) | 41 |
| XmiI | GTMKAC | 1 cut(s) | 61 |