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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 3 cut(s) | 185, 195, 390 |
| AciI | CCGC | 5 cut(s) | 6, 11, 36, 190, 339 |
| AcuI | CTGAAG | 2 cut(s) | 124, 176 |
| AfaI | GTAC | 1 cut(s) | 286 |
| AjnI | CCWGG | 1 cut(s) | 322 |
| AluBI | AGCT | 5 cut(s) | 151, 160, 204, 293, 352 |
| AluI | AGCT | 5 cut(s) | 151, 160, 204, 293, 352 |
| Alw26I | GTCTC | 1 cut(s) | 68 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 154, 293 |
| AoxI | GGCC | 3 cut(s) | 325, 411, 417 |
| ApeKI | GCWGC | 2 cut(s) | 201, 309 |
| AspLEI | GCGC | 4 cut(s) | 89, 185, 195, 390 |
| AspS9I | GGNCC | 1 cut(s) | 8 |
| AsuC2I | CCSGG | 1 cut(s) | 221 |
| AvaII | GGWCC | 1 cut(s) | 8 |
| BanII | GRGCYC | 1 cut(s) | 227 |
| BbvI | GCAGC | 2 cut(s) | 213, 321 |
| BccI | CCATC | 1 cut(s) | 89 |
| BciT130I | CCWGG | 1 cut(s) | 324 |
| BcnI | CCSGG | 1 cut(s) | 221 |
| BcoDI | GTCTC | 1 cut(s) | 68 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 175, 324 |
| BglII | AGATCT | 1 cut(s) | 331 |
| BisI | GCNGC | 4 cut(s) | 191, 202, 310, 339 |
| BlsI | GCNGC | 4 cut(s) | 192, 203, 311, 340 |
| Bme1390I | CCNGG | 2 cut(s) | 221, 324 |
| Bme18I | GGWCC | 1 cut(s) | 8 |
| BmgT120I | GGNCC | 1 cut(s) | 8 |
| BmrFI | CCNGG | 2 cut(s) | 221, 324 |
| BmsI | GCATC | 2 cut(s) | 73, 156 |
| BpmI | CTGGAG | 1 cut(s) | 230 |
| Bpu10I | CCTNAGC | 1 cut(s) | 226 |
| BpuMI | CCSGG | 1 cut(s) | 221 |
| BsaI | GGTCTC | 1 cut(s) | 68 |
| BsaJI | CCNNGG | 2 cut(s) | 220, 414 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 65, 95 |
| BseBI | CCWGG | 1 cut(s) | 324 |
| BseDI | CCNNGG | 2 cut(s) | 220, 414 |
| BseGI | GGATG | 1 cut(s) | 100 |
| BseMII | CTCAG | 2 cut(s) | 240, 285 |
| BseXI | GCAGC | 2 cut(s) | 213, 321 |
| BseYI | CCCAGC | 1 cut(s) | 147 |
| Bsh1236I | CGCG | 3 cut(s) | 185, 195, 390 |
| Bsh1285I | CGRYCG | 1 cut(s) | 305 |
| BshFI | GGCC | 3 cut(s) | 327, 413, 419 |
| BsiEI | CGRYCG | 1 cut(s) | 305 |
| BsiSI | CCGG | 1 cut(s) | 220 |
| BslFI | GGGAC | 3 cut(s) | 129, 141, 283 |
| BsmAI | GTCTC | 1 cut(s) | 68 |
| BsmFI | GGGAC | 3 cut(s) | 129, 141, 283 |
| BsmI | GAATGC | 1 cut(s) | 277 |
| BsnI | GGCC | 3 cut(s) | 327, 413, 419 |
| Bso31I | GGTCTC | 1 cut(s) | 68 |
| Bsp1286I | GDGCHC | 1 cut(s) | 227 |
| Bsp143I | GATC | 5 cut(s) | 103, 121, 302, 331, 364 |
| BspACI | CCGC | 5 cut(s) | 6, 11, 36, 190, 339 |
| BspANI | GGCC | 3 cut(s) | 327, 413, 419 |
| BspCNI | CTCAG | 2 cut(s) | 239, 286 |
| BspFNI | CGCG | 3 cut(s) | 185, 195, 390 |
| BspTNI | GGTCTC | 1 cut(s) | 68 |
| BssECI | CCNNGG | 2 cut(s) | 220, 414 |
| BssMI | GATC | 5 cut(s) | 103, 121, 302, 331, 364 |
| Bst2UI | CCWGG | 1 cut(s) | 324 |
| Bst4CI | ACNGT | 2 cut(s) | 240, 289 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 315 |
| BstC8I | GCNNGC | 2 cut(s) | 217, 354 |
| BstDEI | CTNAG | 3 cut(s) | 26, 226, 294 |
| BstF5I | GGATG | 1 cut(s) | 100 |
| BstFNI | CGCG | 3 cut(s) | 185, 195, 390 |
| BstHHI | GCGC | 4 cut(s) | 89, 185, 195, 390 |
| BstKTI | GATC | 5 cut(s) | 106, 124, 305, 334, 367 |
| BstMAI | GTCTC | 1 cut(s) | 68 |
| BstMBI | GATC | 5 cut(s) | 103, 121, 302, 331, 364 |
| BstMCI | CGRYCG | 1 cut(s) | 305 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 157, 166, 175, 201, 315, 324 |
| BstNI | CCWGG | 1 cut(s) | 324 |
| BstSCI | CCNGG | 2 cut(s) | 219, 322 |
| BstUI | CGCG | 3 cut(s) | 185, 195, 390 |
| BstV1I | GCAGC | 2 cut(s) | 213, 321 |
| BstX2I | RGATCY | 1 cut(s) | 331 |
| BstYI | RGATCY | 1 cut(s) | 331 |
| BsuRI | GGCC | 3 cut(s) | 327, 413, 419 |
| BtsCI | GGATG | 1 cut(s) | 100 |
| Cac8I | GCNNGC | 2 cut(s) | 217, 354 |
| CaiI | CAGNNNCTG | 2 cut(s) | 154, 293 |
| CfoI | GCGC | 4 cut(s) | 89, 185, 195, 390 |
| Cfr13I | GGNCC | 1 cut(s) | 8 |
| CpoI | CGGWCCG | 1 cut(s) | 8 |
| CseI | GACGC | 1 cut(s) | 184 |
| Csp6I | GTAC | 1 cut(s) | 285 |
| CspI | CGGWCCG | 1 cut(s) | 8 |
| CviAII | CATG | 1 cut(s) | 113 |
| CviQI | GTAC | 1 cut(s) | 285 |
| DdeI | CTNAG | 3 cut(s) | 26, 226, 294 |
| DpnI | GATC | 5 cut(s) | 105, 123, 304, 333, 366 |
| DpnII | GATC | 5 cut(s) | 103, 121, 302, 331, 364 |
| EciI | GGCGGA | 1 cut(s) | 21 |
| Eco147I | AGGCCT | 1 cut(s) | 419 |
| Eco24I | GRGCYC | 1 cut(s) | 227 |
| Eco31I | GGTCTC | 1 cut(s) | 68 |
| Eco47I | GGWCC | 1 cut(s) | 8 |
| Eco57I | CTGAAG | 2 cut(s) | 124, 176 |
| EcoRII | CCWGG | 1 cut(s) | 322 |
| EcoT38I | GRGCYC | 1 cut(s) | 227 |
| FaeI | CATG | 1 cut(s) | 116 |
| FaiI | YATR | 5 cut(s) | 114, 263, 279, 348, 372 |
| FaqI | GGGAC | 3 cut(s) | 129, 141, 283 |
| FatI | CATG | 1 cut(s) | 112 |
| FauI | CCCGC | 1 cut(s) | 43 |
| Fnu4HI | GCNGC | 4 cut(s) | 191, 202, 310, 339 |
| FokI | GGATG | 1 cut(s) | 107 |
| FriOI | GRGCYC | 1 cut(s) | 227 |
| Fsp4HI | GCNGC | 4 cut(s) | 191, 202, 310, 339 |
| GlaI | GCGC | 4 cut(s) | 88, 184, 194, 389 |
| GluI | GCNGC | 4 cut(s) | 191, 202, 310, 339 |
| GsaI | CCCAGC | 1 cut(s) | 151 |
| GsuI | CTGGAG | 1 cut(s) | 230 |
| HaeIII | GGCC | 3 cut(s) | 327, 413, 419 |
| HapII | CCGG | 1 cut(s) | 220 |
| HgaI | GACGC | 1 cut(s) | 184 |
| HhaI | GCGC | 4 cut(s) | 89, 185, 195, 390 |
| Hin1II | CATG | 1 cut(s) | 116 |
| Hin6I | GCGC | 4 cut(s) | 87, 183, 193, 388 |
| HinP1I | GCGC | 4 cut(s) | 87, 183, 193, 388 |
| HinfI | GANTC | 1 cut(s) | 296 |
| HpaII | CCGG | 1 cut(s) | 220 |
| Hpy188I | TCNGA | 2 cut(s) | 69, 156 |
| Hpy188III | TCNNGA | 2 cut(s) | 209, 300 |
| HpyAV | CCTTC | 1 cut(s) | 403 |
| HpyCH4III | ACNGT | 2 cut(s) | 240, 289 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 157, 166, 175, 201, 315, 324 |
| HpyF3I | CTNAG | 3 cut(s) | 26, 226, 294 |
| Hsp92II | CATG | 1 cut(s) | 116 |
| HspAI | GCGC | 4 cut(s) | 87, 183, 193, 388 |
| Kzo9I | GATC | 5 cut(s) | 103, 121, 302, 331, 364 |
| LmnI | GCTCC | 1 cut(s) | 385 |
| Lsp1109I | GCAGC | 2 cut(s) | 213, 321 |
| LweI | GCATC | 2 cut(s) | 73, 156 |
| MalI | GATC | 5 cut(s) | 105, 123, 304, 333, 366 |
| MboI | GATC | 5 cut(s) | 103, 121, 302, 331, 364 |
| MboII | GAAGA | 1 cut(s) | 98 |
| MflI | RGATCY | 1 cut(s) | 331 |
| MhlI | GDGCHC | 1 cut(s) | 227 |
| MluCI | AATT | 1 cut(s) | 258 |
| MlyI | GAGTC | 1 cut(s) | 305 |
| MnlI | CCTC | 5 cut(s) | 53, 63, 85, 205, 409 |
| MspI | CCGG | 1 cut(s) | 220 |
| MspR9I | CCNGG | 2 cut(s) | 221, 324 |
| Mva1269I | GAATGC | 1 cut(s) | 277 |
| MvaI | CCWGG | 1 cut(s) | 324 |
| MvnI | CGCG | 3 cut(s) | 185, 195, 390 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 157, 166, 175, 201, 315, 324 |
| NciI | CCSGG | 1 cut(s) | 221 |
| NdeII | GATC | 5 cut(s) | 103, 121, 302, 331, 364 |
| NlaIII | CATG | 1 cut(s) | 116 |
| PceI | AGGCCT | 1 cut(s) | 419 |
| PctI | GAATGC | 1 cut(s) | 277 |
| PkrI | GCNGC | 4 cut(s) | 192, 203, 311, 340 |
| Ple19I | CGATCG | 1 cut(s) | 305 |
| PleI | GAGTC | 1 cut(s) | 304 |
| PpsI | GAGTC | 1 cut(s) | 304 |
| Psp6I | CCWGG | 1 cut(s) | 322 |
| PspFI | CCCAGC | 1 cut(s) | 147 |
| PspGI | CCWGG | 1 cut(s) | 322 |
| PspPI | GGNCC | 1 cut(s) | 8 |
| PstNI | CAGNNNCTG | 2 cut(s) | 154, 293 |
| PsuI | RGATCY | 1 cut(s) | 331 |
| PvuI | CGATCG | 1 cut(s) | 305 |
| RsaI | GTAC | 1 cut(s) | 286 |
| RsaNI | GTAC | 1 cut(s) | 285 |
| Rsr2I | CGGWCCG | 1 cut(s) | 8 |
| RsrII | CGGWCCG | 1 cut(s) | 8 |
| SatI | GCNGC | 4 cut(s) | 191, 202, 310, 339 |
| Sau3AI | GATC | 5 cut(s) | 103, 121, 302, 331, 364 |
| Sau96I | GGNCC | 1 cut(s) | 8 |
| SchI | GAGTC | 1 cut(s) | 305 |
| ScrFI | CCNGG | 2 cut(s) | 221, 324 |
| SduI | GDGCHC | 1 cut(s) | 227 |
| SetI | ASST | 7 cut(s) | 44, 80, 153, 162, 206, 295, 354 |
| SfaNI | GCATC | 2 cut(s) | 73, 156 |
| SinI | GGWCC | 1 cut(s) | 8 |
| Sse9I | AATT | 1 cut(s) | 258 |
| SseBI | AGGCCT | 1 cut(s) | 419 |
| SsiI | CCGC | 5 cut(s) | 6, 11, 36, 190, 339 |
| StuI | AGGCCT | 1 cut(s) | 419 |
| StyD4I | CCNGG | 2 cut(s) | 219, 322 |
| TaaI | ACNGT | 2 cut(s) | 240, 289 |
| TaqI | TCGA | 1 cut(s) | 305 |
| TasI | AATT | 1 cut(s) | 258 |
| TatI | WGTACW | 1 cut(s) | 284 |
| TauI | GCSGC | 2 cut(s) | 193, 341 |
| TseI | GCWGC | 2 cut(s) | 201, 309 |
| VpaK11BI | GGWCC | 1 cut(s) | 8 |