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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 223, 320 |
| Acc65I | GGTACC | 2 cut(s) | 208, 235 |
| AccB1I | GGYRCC | 3 cut(s) | 208, 235, 432 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 383 |
| AccI | GTMKAC | 1 cut(s) | 187 |
| AciI | CCGC | 9 cut(s) | 20, 141, 239, 275, 338, 373, 456, 487, 550 |
| AclWI | GGATC | 2 cut(s) | 30, 410 |
| AcuI | CTGAAG | 1 cut(s) | 140 |
| AcyI | GRCGYC | 1 cut(s) | 433 |
| AfaI | GTAC | 3 cut(s) | 5, 210, 237 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 197, 383, 475 |
| AgsI | TTSAA | 1 cut(s) | 311 |
| AjnI | CCWGG | 2 cut(s) | 176, 473 |
| AluBI | AGCT | 4 cut(s) | 10, 14, 137, 504 |
| AluI | AGCT | 4 cut(s) | 10, 14, 137, 504 |
| AlwI | GGATC | 2 cut(s) | 30, 410 |
| AoxI | GGCC | 4 cut(s) | 75, 215, 349, 477 |
| ApaI | GGGCCC | 1 cut(s) | 481 |
| ApeKI | GCWGC | 1 cut(s) | 489 |
| Asp718I | GGTACC | 2 cut(s) | 208, 235 |
| AspLEI | GCGC | 4 cut(s) | 154, 325, 435, 443 |
| AspS9I | GGNCC | 7 cut(s) | 76, 215, 244, 269, 332, 477, 478 |
| AsuC2I | CCSGG | 3 cut(s) | 192, 213, 420 |
| AsuHPI | GGTGA | 1 cut(s) | 476 |
| AsuNHI | GCTAGC | 1 cut(s) | 10 |
| AvaII | GGWCC | 3 cut(s) | 244, 269, 332 |
| BaeGI | GKGCMC | 1 cut(s) | 481 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 192, 225 |
| BanI | GGYRCC | 3 cut(s) | 208, 235, 432 |
| BanII | GRGCYC | 1 cut(s) | 481 |
| BbvCI | CCTCAGC | 1 cut(s) | 72 |
| BbvI | GCAGC | 1 cut(s) | 476 |
| BccI | CCATC | 2 cut(s) | 259, 287 |
| BciT130I | CCWGG | 2 cut(s) | 178, 475 |
| BcnI | CCSGG | 3 cut(s) | 192, 213, 420 |
| BfaI | CTAG | 1 cut(s) | 11 |
| BfoI | RGCGCY | 2 cut(s) | 436, 444 |
| BfuAI | ACCTGC | 2 cut(s) | 223, 320 |
| BisI | GCNGC | 1 cut(s) | 490 |
| BlsI | GCNGC | 1 cut(s) | 491 |
| Bme1390I | CCNGG | 5 cut(s) | 178, 192, 213, 420, 475 |
| Bme18I | GGWCC | 3 cut(s) | 244, 269, 332 |
| BmgT120I | GGNCC | 7 cut(s) | 76, 215, 244, 269, 332, 477, 478 |
| BmiI | GGNNCC | 7 cut(s) | 210, 216, 237, 246, 270, 434, 479 |
| BmrFI | CCNGG | 5 cut(s) | 178, 192, 213, 420, 475 |
| BmsI | GCATC | 2 cut(s) | 219, 356 |
| BmtI | GCTAGC | 1 cut(s) | 14 |
| BpmI | CTGGAG | 1 cut(s) | 423 |
| Bpu10I | CCTNAGC | 1 cut(s) | 72 |
| BpuMI | CCSGG | 3 cut(s) | 192, 213, 420 |
| BsaAI | YACGTR | 1 cut(s) | 170 |
| BsaHI | GRCGYC | 1 cut(s) | 433 |
| BsaJI | CCNNGG | 2 cut(s) | 212, 474 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 396, 426 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 197, 383, 475 |
| Bse118I | RCCGGY | 1 cut(s) | 360 |
| Bse1I | ACTGG | 3 cut(s) | 202, 218, 406 |
| BseBI | CCWGG | 2 cut(s) | 178, 475 |
| BseDI | CCNNGG | 2 cut(s) | 212, 474 |
| BseGI | GGATG | 3 cut(s) | 270, 279, 397 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 197, 383, 475 |
| BseMII | CTCAG | 3 cut(s) | 63, 243, 462 |
| BseNI | ACTGG | 3 cut(s) | 202, 218, 406 |
| BseRI | GAGGAG | 1 cut(s) | 65 |
| BseSI | GKGCMC | 1 cut(s) | 481 |
| BseXI | GCAGC | 1 cut(s) | 476 |
| BseYI | CCCAGC | 1 cut(s) | 437 |
| BshFI | GGCC | 4 cut(s) | 77, 217, 351, 479 |
| BshNI | GGYRCC | 3 cut(s) | 208, 235, 432 |
| BsiSI | CCGG | 5 cut(s) | 192, 212, 320, 361, 419 |
| BslFI | GGGAC | 1 cut(s) | 282 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 197, 383, 475 |
| BsmFI | GGGAC | 1 cut(s) | 282 |
| BsmI | GAATGC | 1 cut(s) | 62 |
| BsnI | GGCC | 4 cut(s) | 77, 217, 351, 479 |
| Bsp120I | GGGCCC | 1 cut(s) | 477 |
| Bsp1286I | GDGCHC | 1 cut(s) | 481 |
| Bsp143I | GATC | 2 cut(s) | 22, 415 |
| BspACI | CCGC | 9 cut(s) | 20, 141, 239, 275, 338, 373, 456, 487, 550 |
| BspANI | GGCC | 4 cut(s) | 77, 217, 351, 479 |
| BspCNI | CTCAG | 3 cut(s) | 64, 244, 461 |
| BspLI | GGNNCC | 7 cut(s) | 210, 216, 237, 246, 270, 434, 479 |
| BspMI | ACCTGC | 2 cut(s) | 223, 320 |
| BspOI | GCTAGC | 1 cut(s) | 14 |
| BspPI | GGATC | 2 cut(s) | 30, 410 |
| BspT107I | GGYRCC | 3 cut(s) | 208, 235, 432 |
| BsrFI | RCCGGY | 1 cut(s) | 360 |
| BsrI | ACTGG | 3 cut(s) | 202, 218, 406 |
| BssAI | RCCGGY | 1 cut(s) | 360 |
| BssECI | CCNNGG | 2 cut(s) | 212, 474 |
| BssMI | GATC | 2 cut(s) | 22, 415 |
| BssNI | GRCGYC | 1 cut(s) | 433 |
| Bst2UI | CCWGG | 2 cut(s) | 178, 475 |
| Bst4CI | ACNGT | 1 cut(s) | 87 |
| Bst6I | CTCTTC | 1 cut(s) | 116 |
| BstACI | GRCGYC | 1 cut(s) | 433 |
| BstBAI | YACGTR | 1 cut(s) | 170 |
| BstC8I | GCNNGC | 4 cut(s) | 12, 327, 362, 502 |
| BstDEI | CTNAG | 3 cut(s) | 72, 252, 448 |
| BstF5I | GGATG | 3 cut(s) | 270, 279, 397 |
| BstH2I | RGCGCY | 2 cut(s) | 436, 444 |
| BstHHI | GCGC | 4 cut(s) | 154, 325, 435, 443 |
| BstKTI | GATC | 2 cut(s) | 25, 418 |
| BstMBI | GATC | 2 cut(s) | 22, 415 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 149, 238, 335, 357, 366 |
| BstNI | CCWGG | 2 cut(s) | 178, 475 |
| BstSCI | CCNGG | 5 cut(s) | 176, 190, 211, 418, 473 |
| BstSLI | GKGCMC | 1 cut(s) | 481 |
| BstV1I | GCAGC | 1 cut(s) | 476 |
| BstX2I | RGATCY | 1 cut(s) | 22 |
| BstYI | RGATCY | 1 cut(s) | 22 |
| BsuRI | GGCC | 4 cut(s) | 77, 217, 351, 479 |
| BtgZI | GCGATG | 1 cut(s) | 379 |
| BtsCI | GGATG | 3 cut(s) | 270, 279, 397 |
| BtsIMutI | CAGTG | 1 cut(s) | 195 |
| BveI | ACCTGC | 2 cut(s) | 223, 320 |
| Cac8I | GCNNGC | 4 cut(s) | 12, 327, 362, 502 |
| CfoI | GCGC | 4 cut(s) | 154, 325, 435, 443 |
| Cfr10I | RCCGGY | 1 cut(s) | 360 |
| Cfr13I | GGNCC | 7 cut(s) | 76, 215, 244, 269, 332, 477, 478 |
| CseI | GACGC | 1 cut(s) | 136 |
| Csp6I | GTAC | 3 cut(s) | 4, 209, 236 |
| CviQI | GTAC | 3 cut(s) | 4, 209, 236 |
| DdeI | CTNAG | 3 cut(s) | 72, 252, 448 |
| DinI | GGCGCC | 1 cut(s) | 434 |
| DpnI | GATC | 2 cut(s) | 24, 417 |
| DpnII | GATC | 2 cut(s) | 22, 415 |
| Eam1104I | CTCTTC | 1 cut(s) | 116 |
| EarI | CTCTTC | 1 cut(s) | 116 |
| EciI | GGCGGA | 1 cut(s) | 35 |
| Eco24I | GRGCYC | 1 cut(s) | 481 |
| Eco47I | GGWCC | 3 cut(s) | 244, 269, 332 |
| Eco57I | CTGAAG | 1 cut(s) | 140 |
| EcoO109I | RGGNCCY | 1 cut(s) | 478 |
| EcoRII | CCWGG | 2 cut(s) | 176, 473 |
| EcoT38I | GRGCYC | 1 cut(s) | 481 |
| EgeI | GGCGCC | 1 cut(s) | 434 |
| EheI | GGCGCC | 1 cut(s) | 434 |
| FaiI | YATR | 4 cut(s) | 30, 156, 224, 520 |
| FaqI | GGGAC | 1 cut(s) | 282 |
| FblI | GTMKAC | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 1 cut(s) | 490 |
| FokI | GGATG | 3 cut(s) | 266, 277, 404 |
| FriOI | GRGCYC | 1 cut(s) | 481 |
| Fsp4HI | GCNGC | 1 cut(s) | 490 |
| FspBI | CTAG | 1 cut(s) | 11 |
| GlaI | GCGC | 4 cut(s) | 153, 324, 434, 442 |
| GluI | GCNGC | 1 cut(s) | 490 |
| GsaI | CCCAGC | 1 cut(s) | 441 |
| GsuI | CTGGAG | 1 cut(s) | 423 |
| HaeII | RGCGCY | 2 cut(s) | 436, 444 |
| HaeIII | GGCC | 4 cut(s) | 77, 217, 351, 479 |
| HapII | CCGG | 5 cut(s) | 192, 212, 320, 361, 419 |
| HgaI | GACGC | 1 cut(s) | 136 |
| HhaI | GCGC | 4 cut(s) | 154, 325, 435, 443 |
| Hin1I | GRCGYC | 1 cut(s) | 433 |
| Hin6I | GCGC | 4 cut(s) | 152, 323, 433, 441 |
| HinP1I | GCGC | 4 cut(s) | 152, 323, 433, 441 |
| HincII | GTYRAC | 1 cut(s) | 188 |
| HindII | GTYRAC | 1 cut(s) | 188 |
| HinfI | GANTC | 3 cut(s) | 426, 452, 494 |
| HpaII | CCGG | 5 cut(s) | 192, 212, 320, 361, 419 |
| HphI | GGTGA | 1 cut(s) | 476 |
| Hpy166II | GTNNAC | 1 cut(s) | 188 |
| Hpy188I | TCNGA | 2 cut(s) | 451, 560 |
| Hpy188III | TCNNGA | 1 cut(s) | 469 |
| Hpy8I | GTNNAC | 1 cut(s) | 188 |
| HpyAV | CCTTC | 3 cut(s) | 61, 305, 491 |
| HpyCH4III | ACNGT | 1 cut(s) | 87 |
| HpyCH4IV | ACGT | 3 cut(s) | 46, 106, 169 |
| HpyCH4V | TGCA | 5 cut(s) | 60, 232, 369, 500, 522 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 149, 238, 335, 357, 366 |
| HpyF3I | CTNAG | 3 cut(s) | 72, 252, 448 |
| HpySE526I | ACGT | 3 cut(s) | 46, 106, 169 |
| Hsp92I | GRCGYC | 1 cut(s) | 433 |
| HspAI | GCGC | 4 cut(s) | 152, 323, 433, 441 |
| KasI | GGCGCC | 1 cut(s) | 432 |
| KpnI | GGTACC | 2 cut(s) | 212, 239 |
| KroI | GCCGGC | 1 cut(s) | 360 |
| KroNI | GCCGGC | 1 cut(s) | 362 |
| Kzo9I | GATC | 2 cut(s) | 22, 415 |
| LmnI | GCTCC | 1 cut(s) | 404 |
| Lsp1109I | GCAGC | 1 cut(s) | 476 |
| LweI | GCATC | 2 cut(s) | 219, 356 |
| MaeI | CTAG | 1 cut(s) | 11 |
| MaeII | ACGT | 3 cut(s) | 46, 106, 169 |
| MaeIII | GTNAC | 2 cut(s) | 102, 394 |
| MalI | GATC | 2 cut(s) | 24, 417 |
| MboI | GATC | 2 cut(s) | 22, 415 |
| MboII | GAAGA | 2 cut(s) | 17, 133 |
| MflI | RGATCY | 1 cut(s) | 22 |
| MhlI | GDGCHC | 1 cut(s) | 481 |
| Mly113I | GGCGCC | 1 cut(s) | 433 |
| MlyI | GAGTC | 1 cut(s) | 435 |
| MnlI | CCTC | 7 cut(s) | 43, 46, 67, 117, 292, 297, 403 |
| MreI | CGCCGGCG | 1 cut(s) | 360 |
| MroNI | GCCGGC | 1 cut(s) | 360 |
| MslI | CAYNNNNRTG | 1 cut(s) | 227 |
| MspA1I | CMGCKG | 3 cut(s) | 241, 338, 489 |
| MspI | CCGG | 5 cut(s) | 192, 212, 320, 361, 419 |
| MspR9I | CCNGG | 5 cut(s) | 178, 192, 213, 420, 475 |
| Mva1269I | GAATGC | 1 cut(s) | 62 |
| MvaI | CCWGG | 2 cut(s) | 178, 475 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 149, 238, 335, 357, 366 |
| NaeI | GCCGGC | 1 cut(s) | 362 |
| NarI | GGCGCC | 1 cut(s) | 433 |
| NciI | CCSGG | 3 cut(s) | 192, 213, 420 |
| NdeII | GATC | 2 cut(s) | 22, 415 |
| NgoMIV | GCCGGC | 1 cut(s) | 360 |
| NheI | GCTAGC | 1 cut(s) | 10 |
| NlaIV | GGNNCC | 7 cut(s) | 210, 216, 237, 246, 270, 434, 479 |
| NmuCI | GTSAC | 1 cut(s) | 102 |
| PctI | GAATGC | 1 cut(s) | 62 |
| PdiI | GCCGGC | 1 cut(s) | 362 |
| PfeI | GAWTC | 2 cut(s) | 452, 494 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 383 |
| PkrI | GCNGC | 1 cut(s) | 491 |
| PleI | GAGTC | 1 cut(s) | 434 |
| PluTI | GGCGCC | 1 cut(s) | 436 |
| PpsI | GAGTC | 1 cut(s) | 434 |
| Ppu21I | YACGTR | 1 cut(s) | 170 |
| Psp6I | CCWGG | 2 cut(s) | 176, 473 |
| PspFI | CCCAGC | 1 cut(s) | 437 |
| PspGI | CCWGG | 2 cut(s) | 176, 473 |
| PspN4I | GGNNCC | 7 cut(s) | 210, 216, 237, 246, 270, 434, 479 |
| PspOMI | GGGCCC | 1 cut(s) | 477 |
| PspPI | GGNCC | 7 cut(s) | 76, 215, 244, 269, 332, 477, 478 |
| PsuI | RGATCY | 1 cut(s) | 22 |
| RsaI | GTAC | 3 cut(s) | 5, 210, 237 |
| RsaNI | GTAC | 3 cut(s) | 4, 209, 236 |
| RseI | CAYNNNNRTG | 1 cut(s) | 227 |
| SalI | GTCGAC | 1 cut(s) | 186 |
| SatI | GCNGC | 1 cut(s) | 490 |
| Sau3AI | GATC | 2 cut(s) | 22, 415 |
| Sau96I | GGNCC | 7 cut(s) | 76, 215, 244, 269, 332, 477, 478 |
| SchI | GAGTC | 1 cut(s) | 435 |
| ScrFI | CCNGG | 5 cut(s) | 178, 192, 213, 420, 475 |
| SduI | GDGCHC | 1 cut(s) | 481 |
| SfaNI | GCATC | 2 cut(s) | 219, 356 |
| SfoI | GGCGCC | 1 cut(s) | 434 |
| SgrAI | CRCCGGYG | 1 cut(s) | 360 |
| SinI | GGWCC | 3 cut(s) | 244, 269, 332 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 227 |
| SsiI | CCGC | 9 cut(s) | 20, 141, 239, 275, 338, 373, 456, 487, 550 |
| SspDI | GGCGCC | 1 cut(s) | 432 |
| SspI | AATATT | 1 cut(s) | 344 |
| SspMI | CTAG | 1 cut(s) | 11 |
| StyD4I | CCNGG | 5 cut(s) | 176, 190, 211, 418, 473 |
| TaaI | ACNGT | 1 cut(s) | 87 |
| TaiI | ACGT | 3 cut(s) | 49, 109, 172 |
| TaqI | TCGA | 1 cut(s) | 187 |
| TfiI | GAWTC | 2 cut(s) | 452, 494 |
| TscAI | CASTG | 1 cut(s) | 202 |
| TseFI | GTSAC | 1 cut(s) | 102 |
| TseI | GCWGC | 1 cut(s) | 489 |
| Tsp45I | GTSAC | 1 cut(s) | 102 |
| TspDTI | ATGAA | 2 cut(s) | 17, 129 |
| TspRI | CASTG | 1 cut(s) | 202 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 383 |
| VpaK11BI | GGWCC | 3 cut(s) | 244, 269, 332 |
| XmiI | GTMKAC | 1 cut(s) | 187 |
| XspI | CTAG | 1 cut(s) | 11 |