No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 379, 601 |
| AccBSI | CCGCTC | 1 cut(s) | 217 |
| AccI | GTMKAC | 2 cut(s) | 358, 580 |
| AccII | CGCG | 1 cut(s) | 74 |
| AclWI | GGATC | 2 cut(s) | 168, 201 |
| AcyI | GRCGYC | 2 cut(s) | 380, 602 |
| AfaI | GTAC | 3 cut(s) | 176, 408, 630 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 77 |
| AhlI | ACTAGT | 1 cut(s) | 682 |
| AjnI | CCWGG | 8 cut(s) | 133, 156, 249, 347, 421, 471, 569, 643 |
| Alw26I | GTCTC | 1 cut(s) | 433 |
| AlwI | GGATC | 2 cut(s) | 168, 201 |
| AoxI | GGCC | 3 cut(s) | 154, 246, 468 |
| ApeKI | GCWGC | 8 cut(s) | 138, 141, 263, 305, 342, 485, 527, 564 |
| AspLEI | GCGC | 6 cut(s) | 231, 268, 382, 453, 490, 604 |
| AspS9I | GGNCC | 7 cut(s) | 247, 386, 391, 469, 608, 613, 662 |
| AsuC2I | CCSGG | 4 cut(s) | 320, 384, 542, 606 |
| AsuHPI | GGTGA | 1 cut(s) | 134 |
| AvaII | GGWCC | 5 cut(s) | 386, 391, 608, 613, 662 |
| BanI | GGYRCC | 2 cut(s) | 379, 601 |
| BanII | GRGCYC | 1 cut(s) | 45 |
| BbvCI | CCTCAGC | 3 cut(s) | 27, 243, 465 |
| BbvI | GCAGC | 8 cut(s) | 150, 153, 250, 292, 354, 472, 514, 576 |
| BccI | CCATC | 5 cut(s) | 175, 253, 370, 475, 592 |
| BciT130I | CCWGG | 8 cut(s) | 135, 158, 251, 349, 423, 473, 571, 645 |
| BcnI | CCSGG | 4 cut(s) | 320, 384, 542, 606 |
| BcoDI | GTCTC | 1 cut(s) | 433 |
| BcuI | ACTAGT | 1 cut(s) | 682 |
| BfaI | CTAG | 1 cut(s) | 683 |
| BfoI | RGCGCY | 2 cut(s) | 383, 605 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| Bme18I | GGWCC | 5 cut(s) | 386, 391, 608, 613, 662 |
| BmgT120I | GGNCC | 7 cut(s) | 247, 386, 391, 469, 608, 613, 662 |
| BmiI | GGNNCC | 6 cut(s) | 44, 381, 387, 603, 609, 663 |
| Bpu10I | CCTNAGC | 3 cut(s) | 27, 243, 465 |
| BpuMI | CCSGG | 4 cut(s) | 320, 384, 542, 606 |
| BsaHI | GRCGYC | 2 cut(s) | 380, 602 |
| BsaI | GGTCTC | 1 cut(s) | 433 |
| BsaJI | CCNNGG | 4 cut(s) | 249, 422, 471, 644 |
| BsaWI | WCCGGW | 3 cut(s) | 388, 442, 610 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 77 |
| Bse1I | ACTGG | 1 cut(s) | 192 |
| BseBI | CCWGG | 8 cut(s) | 135, 158, 251, 349, 423, 473, 571, 645 |
| BseDI | CCNNGG | 4 cut(s) | 249, 422, 471, 644 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 77 |
| BseMII | CTCAG | 3 cut(s) | 18, 234, 456 |
| BseNI | ACTGG | 1 cut(s) | 192 |
| BseRI | GAGGAG | 4 cut(s) | 44, 236, 239, 461 |
| BseXI | GCAGC | 8 cut(s) | 150, 153, 250, 292, 354, 472, 514, 576 |
| BseYI | CCCAGC | 4 cut(s) | 106, 116, 362, 584 |
| Bsh1236I | CGCG | 1 cut(s) | 74 |
| Bsh1285I | CGRYCG | 1 cut(s) | 78 |
| BshFI | GGCC | 3 cut(s) | 156, 248, 470 |
| BshNI | GGYRCC | 2 cut(s) | 379, 601 |
| BsiEI | CGRYCG | 1 cut(s) | 78 |
| BsiSI | CCGG | 7 cut(s) | 320, 383, 389, 443, 542, 605, 611 |
| BslFI | GGGAC | 1 cut(s) | 675 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 77 |
| BsmAI | GTCTC | 1 cut(s) | 433 |
| BsmFI | GGGAC | 1 cut(s) | 675 |
| BsnI | GGCC | 3 cut(s) | 156, 248, 470 |
| Bso31I | GGTCTC | 1 cut(s) | 433 |
| Bsp1286I | GDGCHC | 1 cut(s) | 45 |
| Bsp143I | GATC | 3 cut(s) | 75, 160, 193 |
| BspANI | GGCC | 3 cut(s) | 156, 248, 470 |
| BspCNI | CTCAG | 3 cut(s) | 19, 235, 457 |
| BspFNI | CGCG | 1 cut(s) | 74 |
| BspLI | GGNNCC | 6 cut(s) | 44, 381, 387, 603, 609, 663 |
| BspPI | GGATC | 2 cut(s) | 168, 201 |
| BspT107I | GGYRCC | 2 cut(s) | 379, 601 |
| BspTNI | GGTCTC | 1 cut(s) | 433 |
| BsrBI | CCGCTC | 1 cut(s) | 217 |
| BsrI | ACTGG | 1 cut(s) | 192 |
| BssECI | CCNNGG | 4 cut(s) | 249, 422, 471, 644 |
| BssMI | GATC | 3 cut(s) | 75, 160, 193 |
| BssNI | GRCGYC | 2 cut(s) | 380, 602 |
| Bst2UI | CCWGG | 8 cut(s) | 135, 158, 251, 349, 423, 473, 571, 645 |
| BstACI | GRCGYC | 2 cut(s) | 380, 602 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 332, 554 |
| BstDEI | CTNAG | 5 cut(s) | 27, 243, 290, 465, 512 |
| BstFNI | CGCG | 1 cut(s) | 74 |
| BstH2I | RGCGCY | 2 cut(s) | 383, 605 |
| BstHHI | GCGC | 6 cut(s) | 231, 268, 382, 453, 490, 604 |
| BstKTI | GATC | 3 cut(s) | 78, 163, 196 |
| BstMAI | GTCTC | 1 cut(s) | 433 |
| BstMBI | GATC | 3 cut(s) | 75, 160, 193 |
| BstMCI | CGRYCG | 1 cut(s) | 78 |
| BstNI | CCWGG | 8 cut(s) | 135, 158, 251, 349, 423, 473, 571, 645 |
| BstNSI | RCATGY | 2 cut(s) | 407, 629 |
| BstUI | CGCG | 1 cut(s) | 74 |
| BstV1I | GCAGC | 8 cut(s) | 150, 153, 250, 292, 354, 472, 514, 576 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 3 cut(s) | 156, 248, 470 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 332, 554 |
| CfoI | GCGC | 6 cut(s) | 231, 268, 382, 453, 490, 604 |
| Cfr13I | GGNCC | 7 cut(s) | 247, 386, 391, 469, 608, 613, 662 |
| Csp6I | GTAC | 3 cut(s) | 175, 407, 629 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 408, 443, 630, 665 |
| CviAII | CATG | 3 cut(s) | 172, 404, 626 |
| CviQI | GTAC | 3 cut(s) | 175, 407, 629 |
| DdeI | CTNAG | 5 cut(s) | 27, 243, 290, 465, 512 |
| DinI | GGCGCC | 2 cut(s) | 381, 603 |
| DpnI | GATC | 3 cut(s) | 77, 162, 195 |
| DpnII | GATC | 3 cut(s) | 75, 160, 193 |
| EciI | GGCGGA | 1 cut(s) | 206 |
| Eco24I | GRGCYC | 1 cut(s) | 45 |
| Eco31I | GGTCTC | 1 cut(s) | 433 |
| Eco47I | GGWCC | 5 cut(s) | 386, 391, 608, 613, 662 |
| EcoRII | CCWGG | 8 cut(s) | 133, 156, 249, 347, 421, 471, 569, 643 |
| EcoT22I | ATGCAT | 2 cut(s) | 405, 627 |
| EcoT38I | GRGCYC | 1 cut(s) | 45 |
| EgeI | GGCGCC | 2 cut(s) | 381, 603 |
| EheI | GGCGCC | 2 cut(s) | 381, 603 |
| FaeI | CATG | 3 cut(s) | 175, 407, 629 |
| FaiI | YATR | 7 cut(s) | 173, 201, 272, 405, 494, 627, 674 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 283, 315, 505, 537 |
| FaqI | GGGAC | 1 cut(s) | 675 |
| FatI | CATG | 3 cut(s) | 171, 403, 625 |
| FauI | CCCGC | 4 cut(s) | 8, 55, 79, 210 |
| FblI | GTMKAC | 2 cut(s) | 358, 580 |
| FriOI | GRGCYC | 1 cut(s) | 45 |
| FspBI | CTAG | 1 cut(s) | 683 |
| GlaI | GCGC | 6 cut(s) | 230, 267, 381, 452, 489, 603 |
| GsaI | CCCAGC | 4 cut(s) | 110, 120, 366, 588 |
| HaeII | RGCGCY | 2 cut(s) | 383, 605 |
| HaeIII | GGCC | 3 cut(s) | 156, 248, 470 |
| HapII | CCGG | 7 cut(s) | 320, 383, 389, 443, 542, 605, 611 |
| HhaI | GCGC | 6 cut(s) | 231, 268, 382, 453, 490, 604 |
| Hin1I | GRCGYC | 2 cut(s) | 380, 602 |
| Hin1II | CATG | 3 cut(s) | 175, 407, 629 |
| Hin6I | GCGC | 6 cut(s) | 229, 266, 380, 451, 488, 602 |
| HinP1I | GCGC | 6 cut(s) | 229, 266, 380, 451, 488, 602 |
| HincII | GTYRAC | 3 cut(s) | 85, 359, 581 |
| HindII | GTYRAC | 3 cut(s) | 85, 359, 581 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 7 cut(s) | 320, 383, 389, 443, 542, 605, 611 |
| HphI | GGTGA | 1 cut(s) | 134 |
| Hpy166II | GTNNAC | 3 cut(s) | 85, 359, 581 |
| Hpy188I | TCNGA | 1 cut(s) | 67 |
| Hpy188III | TCNNGA | 2 cut(s) | 432, 654 |
| Hpy8I | GTNNAC | 3 cut(s) | 85, 359, 581 |
| HpyAV | CCTTC | 2 cut(s) | 232, 454 |
| HpyCH4IV | ACGT | 2 cut(s) | 277, 499 |
| HpyCH4V | TGCA | 2 cut(s) | 403, 625 |
| HpyF3I | CTNAG | 5 cut(s) | 27, 243, 290, 465, 512 |
| HpySE526I | ACGT | 2 cut(s) | 277, 499 |
| Hsp92I | GRCGYC | 2 cut(s) | 380, 602 |
| Hsp92II | CATG | 3 cut(s) | 175, 407, 629 |
| HspAI | GCGC | 6 cut(s) | 229, 266, 380, 451, 488, 602 |
| KasI | GGCGCC | 2 cut(s) | 379, 601 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 3 cut(s) | 75, 160, 193 |
| LmnI | GCTCC | 5 cut(s) | 31, 48, 214, 226, 448 |
| Lsp1109I | GCAGC | 8 cut(s) | 150, 153, 250, 292, 354, 472, 514, 576 |
| MaeI | CTAG | 1 cut(s) | 683 |
| MaeII | ACGT | 2 cut(s) | 277, 499 |
| MaeIII | GTNAC | 2 cut(s) | 336, 558 |
| MalI | GATC | 3 cut(s) | 77, 162, 195 |
| MbiI | CCGCTC | 1 cut(s) | 217 |
| MboI | GATC | 3 cut(s) | 75, 160, 193 |
| MboII | GAAGA | 1 cut(s) | 188 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 1 cut(s) | 45 |
| Mly113I | GGCGCC | 2 cut(s) | 380, 602 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| Mph1103I | ATGCAT | 2 cut(s) | 405, 627 |
| MseI | TTAA | 3 cut(s) | 84, 414, 636 |
| MspA1I | CMGCKG | 1 cut(s) | 120 |
| MspI | CCGG | 7 cut(s) | 320, 383, 389, 443, 542, 605, 611 |
| MvaI | CCWGG | 8 cut(s) | 135, 158, 251, 349, 423, 473, 571, 645 |
| MvnI | CGCG | 1 cut(s) | 74 |
| NarI | GGCGCC | 2 cut(s) | 380, 602 |
| NciI | CCSGG | 4 cut(s) | 320, 384, 542, 606 |
| NdeII | GATC | 3 cut(s) | 75, 160, 193 |
| NlaIII | CATG | 3 cut(s) | 175, 407, 629 |
| NlaIV | GGNNCC | 6 cut(s) | 44, 381, 387, 603, 609, 663 |
| NmuCI | GTSAC | 2 cut(s) | 336, 558 |
| NsiI | ATGCAT | 2 cut(s) | 405, 627 |
| NspI | RCATGY | 2 cut(s) | 407, 629 |
| Ple19I | CGATCG | 1 cut(s) | 78 |
| PluTI | GGCGCC | 2 cut(s) | 383, 605 |
| Psp6I | CCWGG | 8 cut(s) | 133, 156, 249, 347, 421, 471, 569, 643 |
| PspFI | CCCAGC | 4 cut(s) | 106, 116, 362, 584 |
| PspGI | CCWGG | 8 cut(s) | 133, 156, 249, 347, 421, 471, 569, 643 |
| PspN4I | GGNNCC | 6 cut(s) | 44, 381, 387, 603, 609, 663 |
| PspPI | GGNCC | 7 cut(s) | 247, 386, 391, 469, 608, 613, 662 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PvuI | CGATCG | 1 cut(s) | 78 |
| RsaI | GTAC | 3 cut(s) | 176, 408, 630 |
| RsaNI | GTAC | 3 cut(s) | 175, 407, 629 |
| SalI | GTCGAC | 2 cut(s) | 357, 579 |
| SaqAI | TTAA | 3 cut(s) | 84, 414, 636 |
| Sau3AI | GATC | 3 cut(s) | 75, 160, 193 |
| Sau96I | GGNCC | 7 cut(s) | 247, 386, 391, 469, 608, 613, 662 |
| SduI | GDGCHC | 1 cut(s) | 45 |
| SetI | ASST | 2 cut(s) | 280, 502 |
| SfoI | GGCGCC | 2 cut(s) | 381, 603 |
| SinI | GGWCC | 5 cut(s) | 386, 391, 608, 613, 662 |
| SpeI | ACTAGT | 1 cut(s) | 682 |
| SspDI | GGCGCC | 2 cut(s) | 379, 601 |
| SspMI | CTAG | 1 cut(s) | 683 |
| TaiI | ACGT | 2 cut(s) | 280, 502 |
| TaqI | TCGA | 4 cut(s) | 282, 358, 504, 580 |
| TauI | GCSGC | 2 cut(s) | 147, 670 |
| Tru1I | TTAA | 3 cut(s) | 84, 414, 636 |
| Tru9I | TTAA | 3 cut(s) | 84, 414, 636 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 2 cut(s) | 336, 558 |
| TseI | GCWGC | 8 cut(s) | 138, 141, 263, 305, 342, 485, 527, 564 |
| Tsp45I | GTSAC | 2 cut(s) | 336, 558 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspRI | CASTG | 1 cut(s) | 192 |
| VpaK11BI | GGWCC | 5 cut(s) | 386, 391, 608, 613, 662 |
| XceI | RCATGY | 2 cut(s) | 407, 629 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 178 |
| XmiI | GTMKAC | 2 cut(s) | 358, 580 |
| XspI | CTAG | 1 cut(s) | 683 |
| Zsp2I | ATGCAT | 2 cut(s) | 405, 627 |