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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 400 |
| Acc36I | ACCTGC | 2 cut(s) | 247, 400 |
| AccB1I | GGYRCC | 1 cut(s) | 429 |
| AccBSI | CCGCTC | 1 cut(s) | 357 |
| AccII | CGCG | 1 cut(s) | 90 |
| AclWI | GGATC | 3 cut(s) | 201, 415, 485 |
| AcoI | YGGCCR | 1 cut(s) | 48 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AcyI | GRCGYC | 1 cut(s) | 430 |
| AfaI | GTAC | 1 cut(s) | 516 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 16, 256, 361, 404 |
| AgsI | TTSAA | 1 cut(s) | 448 |
| AjnI | CCWGG | 5 cut(s) | 115, 132, 398, 425, 470 |
| AluBI | AGCT | 3 cut(s) | 181, 280, 313 |
| AluI | AGCT | 3 cut(s) | 181, 280, 313 |
| Alw26I | GTCTC | 1 cut(s) | 119 |
| AlwI | GGATC | 3 cut(s) | 201, 415, 485 |
| Ama87I | CYCGRG | 1 cut(s) | 358 |
| AoxI | GGCC | 4 cut(s) | 48, 154, 246, 507 |
| ApeKI | GCWGC | 3 cut(s) | 141, 343, 486 |
| AspLEI | GCGC | 3 cut(s) | 92, 432, 440 |
| AspS9I | GGNCC | 3 cut(s) | 247, 259, 474 |
| AsuC2I | CCSGG | 2 cut(s) | 71, 107 |
| AsuHPI | GGTGA | 3 cut(s) | 134, 157, 473 |
| AvaI | CYCGRG | 1 cut(s) | 358 |
| AvaII | GGWCC | 2 cut(s) | 259, 474 |
| BaeGI | GKGCMC | 1 cut(s) | 309 |
| BanI | GGYRCC | 1 cut(s) | 429 |
| BbvI | GCAGC | 3 cut(s) | 153, 330, 473 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 27, 61, 321, 355 |
| BciT130I | CCWGG | 5 cut(s) | 117, 134, 400, 427, 472 |
| BcnI | CCSGG | 2 cut(s) | 71, 107 |
| BcoDI | GTCTC | 1 cut(s) | 119 |
| BfaI | CTAG | 1 cut(s) | 366 |
| BfmI | CTRYAG | 1 cut(s) | 495 |
| BfoI | RGCGCY | 2 cut(s) | 433, 441 |
| BfuAI | ACCTGC | 2 cut(s) | 247, 400 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 4 cut(s) | 142, 145, 344, 487 |
| BlsI | GCNGC | 4 cut(s) | 143, 146, 345, 488 |
| Bme1390I | CCNGG | 7 cut(s) | 71, 107, 117, 134, 400, 427, 472 |
| Bme18I | GGWCC | 2 cut(s) | 259, 474 |
| BmeT110I | CYCGRG | 1 cut(s) | 358 |
| BmgT120I | GGNCC | 3 cut(s) | 247, 259, 474 |
| BmiI | GGNNCC | 3 cut(s) | 261, 294, 431 |
| BmrFI | CCNGG | 7 cut(s) | 71, 107, 117, 134, 400, 427, 472 |
| BmsI | GCATC | 1 cut(s) | 471 |
| BpuMI | CCSGG | 2 cut(s) | 71, 107 |
| BsaHI | GRCGYC | 1 cut(s) | 430 |
| BsaJI | CCNNGG | 6 cut(s) | 105, 115, 133, 243, 378, 398 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 16, 256, 361, 404 |
| Bse1I | ACTGG | 1 cut(s) | 43 |
| BseBI | CCWGG | 5 cut(s) | 117, 134, 400, 427, 472 |
| BseDI | CCNNGG | 6 cut(s) | 105, 115, 133, 243, 378, 398 |
| BseGI | GGATG | 1 cut(s) | 462 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 16, 256, 361, 404 |
| BseNI | ACTGG | 1 cut(s) | 43 |
| BseSI | GKGCMC | 1 cut(s) | 309 |
| BseXI | GCAGC | 3 cut(s) | 153, 330, 473 |
| BseYI | CCCAGC | 2 cut(s) | 309, 434 |
| BsgI | GTGCAG | 1 cut(s) | 329 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| BshFI | GGCC | 4 cut(s) | 50, 156, 248, 509 |
| BshNI | GGYRCC | 1 cut(s) | 429 |
| BsiHKCI | CYCGRG | 1 cut(s) | 358 |
| BsiSI | CCGG | 2 cut(s) | 71, 107 |
| BsiWI | CGTACG | 1 cut(s) | 514 |
| BslFI | GGGAC | 2 cut(s) | 245, 521 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 16, 256, 361, 404 |
| BsmAI | GTCTC | 1 cut(s) | 119 |
| BsmFI | GGGAC | 2 cut(s) | 245, 521 |
| BsmI | GAATGC | 1 cut(s) | 427 |
| BsnI | GGCC | 4 cut(s) | 50, 156, 248, 509 |
| BsoBI | CYCGRG | 1 cut(s) | 358 |
| Bsp1286I | GDGCHC | 1 cut(s) | 309 |
| Bsp143I | GATC | 3 cut(s) | 193, 407, 490 |
| BspANI | GGCC | 4 cut(s) | 50, 156, 248, 509 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspLI | GGNNCC | 3 cut(s) | 261, 294, 431 |
| BspMI | ACCTGC | 2 cut(s) | 247, 400 |
| BspPI | GGATC | 3 cut(s) | 201, 415, 485 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 429 |
| BsrBI | CCGCTC | 1 cut(s) | 357 |
| BsrI | ACTGG | 1 cut(s) | 43 |
| BssECI | CCNNGG | 6 cut(s) | 105, 115, 133, 243, 378, 398 |
| BssMI | GATC | 3 cut(s) | 193, 407, 490 |
| BssNI | GRCGYC | 1 cut(s) | 430 |
| Bst2UI | CCWGG | 5 cut(s) | 117, 134, 400, 427, 472 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstACI | GRCGYC | 1 cut(s) | 430 |
| BstC8I | GCNNGC | 3 cut(s) | 36, 183, 511 |
| BstF5I | GGATG | 1 cut(s) | 462 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstH2I | RGCGCY | 2 cut(s) | 433, 441 |
| BstHHI | GCGC | 3 cut(s) | 92, 432, 440 |
| BstKTI | GATC | 3 cut(s) | 196, 410, 493 |
| BstMAI | GTCTC | 1 cut(s) | 119 |
| BstMBI | GATC | 3 cut(s) | 193, 407, 490 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 153, 262 |
| BstNI | CCWGG | 5 cut(s) | 117, 134, 400, 427, 472 |
| BstSCI | CCNGG | 7 cut(s) | 69, 105, 115, 132, 398, 425, 470 |
| BstSFI | CTRYAG | 1 cut(s) | 495 |
| BstSLI | GKGCMC | 1 cut(s) | 309 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 3 cut(s) | 153, 330, 473 |
| BstX2I | RGATCY | 1 cut(s) | 193 |
| BstYI | RGATCY | 1 cut(s) | 193 |
| BsuRI | GGCC | 4 cut(s) | 50, 156, 248, 509 |
| BtgZI | GCGATG | 1 cut(s) | 324 |
| BtsCI | GGATG | 1 cut(s) | 462 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| BveI | ACCTGC | 2 cut(s) | 247, 400 |
| Cac8I | GCNNGC | 3 cut(s) | 36, 183, 511 |
| CfoI | GCGC | 3 cut(s) | 92, 432, 440 |
| Cfr13I | GGNCC | 3 cut(s) | 247, 259, 474 |
| Csp6I | GTAC | 1 cut(s) | 515 |
| CviQI | GTAC | 1 cut(s) | 515 |
| DinI | GGCGCC | 1 cut(s) | 431 |
| DpnI | GATC | 3 cut(s) | 195, 409, 492 |
| DpnII | GATC | 3 cut(s) | 193, 407, 490 |
| EaeI | YGGCCR | 1 cut(s) | 48 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| Eco47I | GGWCC | 2 cut(s) | 259, 474 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| Eco88I | CYCGRG | 1 cut(s) | 358 |
| EcoO109I | RGGNCCY | 1 cut(s) | 259 |
| EcoRII | CCWGG | 5 cut(s) | 115, 132, 398, 425, 470 |
| EgeI | GGCGCC | 1 cut(s) | 431 |
| EheI | GGCGCC | 1 cut(s) | 431 |
| FaiI | YATR | 3 cut(s) | 175, 177, 552 |
| FaqI | GGGAC | 2 cut(s) | 245, 521 |
| FauI | CCCGC | 2 cut(s) | 8, 270 |
| Fnu4HI | GCNGC | 4 cut(s) | 142, 145, 344, 487 |
| FokI | GGATG | 1 cut(s) | 449 |
| Fsp4HI | GCNGC | 4 cut(s) | 142, 145, 344, 487 |
| FspBI | CTAG | 1 cut(s) | 366 |
| GlaI | GCGC | 3 cut(s) | 91, 431, 439 |
| GluI | GCNGC | 4 cut(s) | 142, 145, 344, 487 |
| GsaI | CCCAGC | 2 cut(s) | 313, 438 |
| HaeII | RGCGCY | 2 cut(s) | 433, 441 |
| HaeIII | GGCC | 4 cut(s) | 50, 156, 248, 509 |
| HapII | CCGG | 2 cut(s) | 71, 107 |
| HhaI | GCGC | 3 cut(s) | 92, 432, 440 |
| Hin1I | GRCGYC | 1 cut(s) | 430 |
| Hin6I | GCGC | 3 cut(s) | 90, 430, 438 |
| HinP1I | GCGC | 3 cut(s) | 90, 430, 438 |
| HinfI | GANTC | 2 cut(s) | 335, 524 |
| HpaII | CCGG | 2 cut(s) | 71, 107 |
| HphI | GGTGA | 3 cut(s) | 134, 157, 473 |
| Hpy166II | GTNNAC | 3 cut(s) | 79, 112, 221 |
| Hpy8I | GTNNAC | 3 cut(s) | 79, 112, 221 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| HpyCH4IV | ACGT | 1 cut(s) | 217 |
| HpyCH4V | TGCA | 1 cut(s) | 346 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 153, 262 |
| HpySE526I | ACGT | 1 cut(s) | 217 |
| Hsp92I | GRCGYC | 1 cut(s) | 430 |
| HspAI | GCGC | 3 cut(s) | 90, 430, 438 |
| KasI | GGCGCC | 1 cut(s) | 429 |
| Kzo9I | GATC | 3 cut(s) | 193, 407, 490 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 3 cut(s) | 292, 322, 400 |
| Lsp1109I | GCAGC | 3 cut(s) | 153, 330, 473 |
| LweI | GCATC | 1 cut(s) | 471 |
| MaeI | CTAG | 1 cut(s) | 366 |
| MaeII | ACGT | 1 cut(s) | 217 |
| MalI | GATC | 3 cut(s) | 195, 409, 492 |
| MbiI | CCGCTC | 1 cut(s) | 357 |
| MboI | GATC | 3 cut(s) | 193, 407, 490 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MflI | RGATCY | 1 cut(s) | 193 |
| MhlI | GDGCHC | 1 cut(s) | 309 |
| MluCI | AATT | 1 cut(s) | 442 |
| Mly113I | GGCGCC | 1 cut(s) | 430 |
| MlyI | GAGTC | 1 cut(s) | 518 |
| MmeI | TCCRAC | 1 cut(s) | 300 |
| MnlI | CCTC | 7 cut(s) | 173, 178, 238, 322, 354, 373, 398 |
| MspA1I | CMGCKG | 4 cut(s) | 265, 418, 486, 547 |
| MspI | CCGG | 2 cut(s) | 71, 107 |
| MspR9I | CCNGG | 7 cut(s) | 71, 107, 117, 134, 400, 427, 472 |
| Mva1269I | GAATGC | 1 cut(s) | 427 |
| MvaI | CCWGG | 5 cut(s) | 117, 134, 400, 427, 472 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 153, 262 |
| NarI | GGCGCC | 1 cut(s) | 430 |
| NciI | CCSGG | 2 cut(s) | 71, 107 |
| NdeII | GATC | 3 cut(s) | 193, 407, 490 |
| NlaIV | GGNNCC | 3 cut(s) | 261, 294, 431 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| PaeR7I | CTCGAG | 1 cut(s) | 358 |
| PaqCI | CACCTGC | 1 cut(s) | 400 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PctI | GAATGC | 1 cut(s) | 427 |
| PfeI | GAWTC | 1 cut(s) | 335 |
| Pfl23II | CGTACG | 1 cut(s) | 514 |
| PkrI | GCNGC | 4 cut(s) | 143, 146, 345, 488 |
| PleI | GAGTC | 1 cut(s) | 518 |
| PluTI | GGCGCC | 1 cut(s) | 433 |
| PpsI | GAGTC | 1 cut(s) | 518 |
| PpuMI | RGGWCCY | 1 cut(s) | 259 |
| Psp5II | RGGWCCY | 1 cut(s) | 259 |
| Psp6I | CCWGG | 5 cut(s) | 115, 132, 398, 425, 470 |
| PspFI | CCCAGC | 2 cut(s) | 309, 434 |
| PspGI | CCWGG | 5 cut(s) | 115, 132, 398, 425, 470 |
| PspLI | CGTACG | 1 cut(s) | 514 |
| PspN4I | GGNNCC | 3 cut(s) | 261, 294, 431 |
| PspPI | GGNCC | 3 cut(s) | 247, 259, 474 |
| PspPPI | RGGWCCY | 1 cut(s) | 259 |
| PspXI | VCTCGAGB | 1 cut(s) | 358 |
| PsuI | RGATCY | 1 cut(s) | 193 |
| RsaI | GTAC | 1 cut(s) | 516 |
| RsaNI | GTAC | 1 cut(s) | 515 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SatI | GCNGC | 4 cut(s) | 142, 145, 344, 487 |
| Sau3AI | GATC | 3 cut(s) | 193, 407, 490 |
| Sau96I | GGNCC | 3 cut(s) | 247, 259, 474 |
| SchI | GAGTC | 1 cut(s) | 518 |
| ScrFI | CCNGG | 7 cut(s) | 71, 107, 117, 134, 400, 427, 472 |
| SduI | GDGCHC | 1 cut(s) | 309 |
| SetI | ASST | 8 cut(s) | 165, 170, 183, 220, 261, 282, 315, 394 |
| SfaNI | GCATC | 1 cut(s) | 471 |
| SfcI | CTRYAG | 1 cut(s) | 495 |
| SfoI | GGCGCC | 1 cut(s) | 431 |
| Sfr274I | CTCGAG | 1 cut(s) | 358 |
| SinI | GGWCC | 2 cut(s) | 259, 474 |
| SlaI | CTCGAG | 1 cut(s) | 358 |
| SmlI | CTYRAG | 1 cut(s) | 358 |
| SmoI | CTYRAG | 1 cut(s) | 358 |
| Sse9I | AATT | 1 cut(s) | 442 |
| SspDI | GGCGCC | 1 cut(s) | 429 |
| SspMI | CTAG | 1 cut(s) | 366 |
| StyD4I | CCNGG | 7 cut(s) | 69, 105, 115, 132, 398, 425, 470 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaiI | ACGT | 1 cut(s) | 220 |
| TaqI | TCGA | 1 cut(s) | 359 |
| TasI | AATT | 1 cut(s) | 442 |
| TauI | GCSGC | 1 cut(s) | 147 |
| TfiI | GAWTC | 1 cut(s) | 335 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseI | GCWGC | 3 cut(s) | 141, 343, 486 |
| TspGWI | ACGGA | 1 cut(s) | 206 |
| TspRI | CASTG | 1 cut(s) | 192 |
| VpaK11BI | GGWCC | 2 cut(s) | 259, 474 |
| XhoI | CTCGAG | 1 cut(s) | 358 |
| XspI | CTAG | 1 cut(s) | 366 |