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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 30 |
| AfeI | AGCGCT | 1 cut(s) | 59 |
| AgsI | TTSAA | 1 cut(s) | 21 |
| AjnI | CCWGG | 1 cut(s) | 163 |
| AluBI | AGCT | 4 cut(s) | 94, 175, 183, 189 |
| AluI | AGCT | 4 cut(s) | 94, 175, 183, 189 |
| Alw21I | GWGCWC | 1 cut(s) | 75 |
| Alw44I | GTGCAC | 1 cut(s) | 71 |
| Aor51HI | AGCGCT | 1 cut(s) | 59 |
| AoxI | GGCC | 1 cut(s) | 191 |
| ApaLI | GTGCAC | 1 cut(s) | 71 |
| ApeKI | GCWGC | 1 cut(s) | 175 |
| AspLEI | GCGC | 1 cut(s) | 60 |
| BaeGI | GKGCMC | 1 cut(s) | 75 |
| Bbv12I | GWGCWC | 1 cut(s) | 75 |
| BbvCI | CCTCAGC | 1 cut(s) | 54 |
| BbvI | GCAGC | 1 cut(s) | 162 |
| BccI | CCATC | 2 cut(s) | 53, 97 |
| BciT130I | CCWGG | 1 cut(s) | 165 |
| BfoI | RGCGCY | 1 cut(s) | 61 |
| BisI | GCNGC | 1 cut(s) | 176 |
| BlsI | GCNGC | 1 cut(s) | 177 |
| Bme1390I | CCNGG | 1 cut(s) | 165 |
| BmrFI | CCNGG | 1 cut(s) | 165 |
| Bpu10I | CCTNAGC | 1 cut(s) | 54 |
| BpuEI | CTTGAG | 1 cut(s) | 205 |
| BsaJI | CCNNGG | 2 cut(s) | 164, 194 |
| Bse1I | ACTGG | 1 cut(s) | 80 |
| BseBI | CCWGG | 1 cut(s) | 165 |
| BseDI | CCNNGG | 2 cut(s) | 164, 194 |
| BseMII | CTCAG | 1 cut(s) | 68 |
| BseNI | ACTGG | 1 cut(s) | 80 |
| BseSI | GKGCMC | 1 cut(s) | 75 |
| BseXI | GCAGC | 1 cut(s) | 162 |
| BseYI | CCCAGC | 1 cut(s) | 90 |
| BshFI | GGCC | 1 cut(s) | 193 |
| BsiHKAI | GWGCWC | 1 cut(s) | 75 |
| BsnI | GGCC | 1 cut(s) | 193 |
| Bsp1286I | GDGCHC | 1 cut(s) | 75 |
| Bsp143I | GATC | 1 cut(s) | 13 |
| BspACI | CCGC | 1 cut(s) | 30 |
| BspANI | GGCC | 1 cut(s) | 193 |
| BspCNI | CTCAG | 1 cut(s) | 67 |
| BsrI | ACTGG | 1 cut(s) | 80 |
| BssECI | CCNNGG | 2 cut(s) | 164, 194 |
| BssMI | GATC | 1 cut(s) | 13 |
| BssT1I | CCWWGG | 1 cut(s) | 194 |
| Bst2UI | CCWGG | 1 cut(s) | 165 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 175 |
| BstC8I | GCNNGC | 2 cut(s) | 30, 191 |
| BstDEI | CTNAG | 1 cut(s) | 54 |
| BstH2I | RGCGCY | 1 cut(s) | 61 |
| BstHHI | GCGC | 1 cut(s) | 60 |
| BstKTI | GATC | 1 cut(s) | 16 |
| BstMBI | GATC | 1 cut(s) | 13 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 175 |
| BstNI | CCWGG | 1 cut(s) | 165 |
| BstSCI | CCNGG | 1 cut(s) | 163 |
| BstSLI | GKGCMC | 1 cut(s) | 75 |
| BstV1I | GCAGC | 1 cut(s) | 162 |
| BsuRI | GGCC | 1 cut(s) | 193 |
| BtsIMutI | CAGTG | 2 cut(s) | 73, 98 |
| Cac8I | GCNNGC | 2 cut(s) | 30, 191 |
| CfoI | GCGC | 1 cut(s) | 60 |
| CviJI | RGCY | 6 cut(s) | 28, 94, 175, 183, 189, 193 |
| CviKI_1 | RGCY | 6 cut(s) | 28, 94, 175, 183, 189, 193 |
| DdeI | CTNAG | 1 cut(s) | 54 |
| DpnI | GATC | 1 cut(s) | 15 |
| DpnII | GATC | 1 cut(s) | 13 |
| Eco130I | CCWWGG | 1 cut(s) | 194 |
| Eco47III | AGCGCT | 1 cut(s) | 59 |
| EcoRII | CCWGG | 1 cut(s) | 163 |
| EcoT14I | CCWWGG | 1 cut(s) | 194 |
| ErhI | CCWWGG | 1 cut(s) | 194 |
| FaiI | YATR | 1 cut(s) | 64 |
| FauI | CCCGC | 1 cut(s) | 37 |
| Fnu4HI | GCNGC | 1 cut(s) | 176 |
| Fsp4HI | GCNGC | 1 cut(s) | 176 |
| GlaI | GCGC | 1 cut(s) | 59 |
| GluI | GCNGC | 1 cut(s) | 176 |
| GsaI | CCCAGC | 1 cut(s) | 94 |
| HaeII | RGCGCY | 1 cut(s) | 61 |
| HaeIII | GGCC | 1 cut(s) | 193 |
| HhaI | GCGC | 1 cut(s) | 60 |
| Hin6I | GCGC | 1 cut(s) | 58 |
| HinP1I | GCGC | 1 cut(s) | 58 |
| HincII | GTYRAC | 1 cut(s) | 109 |
| HindII | GTYRAC | 1 cut(s) | 109 |
| HindIII | AAGCTT | 1 cut(s) | 181 |
| Hpy166II | GTNNAC | 3 cut(s) | 73, 109, 201 |
| Hpy8I | GTNNAC | 3 cut(s) | 73, 109, 201 |
| HpyCH4V | TGCA | 1 cut(s) | 73 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 175 |
| HpyF3I | CTNAG | 1 cut(s) | 54 |
| HspAI | GCGC | 1 cut(s) | 58 |
| Kzo9I | GATC | 1 cut(s) | 13 |
| LpnPI | CCDG | 7 cut(s) | 61, 104, 150, 160, 175, 177, 217 |
| Lsp1109I | GCAGC | 1 cut(s) | 162 |
| MaeIII | GTNAC | 1 cut(s) | 120 |
| MalI | GATC | 1 cut(s) | 15 |
| MboI | GATC | 1 cut(s) | 13 |
| MhlI | GDGCHC | 1 cut(s) | 75 |
| MnlI | CCTC | 3 cut(s) | 48, 63, 149 |
| MspR9I | CCNGG | 1 cut(s) | 165 |
| MvaI | CCWGG | 1 cut(s) | 165 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 175 |
| NdeII | GATC | 1 cut(s) | 13 |
| NmuCI | GTSAC | 1 cut(s) | 120 |
| PkrI | GCNGC | 1 cut(s) | 177 |
| Psp6I | CCWGG | 1 cut(s) | 163 |
| PspFI | CCCAGC | 1 cut(s) | 90 |
| PspGI | CCWGG | 1 cut(s) | 163 |
| SatI | GCNGC | 1 cut(s) | 176 |
| Sau3AI | GATC | 1 cut(s) | 13 |
| ScrFI | CCNGG | 1 cut(s) | 165 |
| SduI | GDGCHC | 1 cut(s) | 75 |
| SetI | ASST | 6 cut(s) | 96, 149, 177, 185, 191, 206 |
| SmlI | CTYRAG | 1 cut(s) | 184 |
| SmoI | CTYRAG | 1 cut(s) | 184 |
| SsiI | CCGC | 1 cut(s) | 30 |
| StyD4I | CCNGG | 1 cut(s) | 163 |
| StyI | CCWWGG | 1 cut(s) | 194 |
| TaqI | TCGA | 2 cut(s) | 12, 83 |
| TscAI | CASTG | 2 cut(s) | 80, 105 |
| TseFI | GTSAC | 1 cut(s) | 120 |
| TseI | GCWGC | 1 cut(s) | 175 |
| Tsp45I | GTSAC | 1 cut(s) | 120 |
| TspRI | CASTG | 2 cut(s) | 80, 105 |
| VneI | GTGCAC | 1 cut(s) | 71 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 186 |