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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 879 |
| Acc36I | ACCTGC | 1 cut(s) | 556 |
| AccB1I | GGYRCC | 1 cut(s) | 379 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccBSI | CCGCTC | 1 cut(s) | 504 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 2 cut(s) | 74, 90 |
| AclWI | GGATC | 9 cut(s) | 168, 201, 527, 637, 782, 961, 986, 1044, 1144 |
| AcoI | YGGCCR | 3 cut(s) | 108, 154, 306 |
| AcuI | CTGAAG | 2 cut(s) | 48, 264 |
| AcyI | GRCGYC | 3 cut(s) | 380, 668, 1111 |
| AfaI | GTAC | 3 cut(s) | 176, 396, 408 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 942 |
| AgsI | TTSAA | 3 cut(s) | 415, 814, 1166 |
| AjnI | CCWGG | 7 cut(s) | 133, 156, 347, 382, 421, 512, 1064 |
| AleI | CACNNNNGTG | 1 cut(s) | 302 |
| AluBI | AGCT | 4 cut(s) | 43, 607, 674, 1126 |
| AluI | AGCT | 4 cut(s) | 43, 607, 674, 1126 |
| Alw21I | GWGCWC | 2 cut(s) | 45, 347 |
| Alw26I | GTCTC | 2 cut(s) | 647, 1150 |
| AlwI | GGATC | 9 cut(s) | 168, 201, 527, 637, 782, 961, 986, 1044, 1144 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1056 |
| AoxI | GGCC | 5 cut(s) | 108, 154, 306, 576, 1134 |
| ApeKI | GCWGC | 4 cut(s) | 138, 141, 263, 1050 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 486 |
| AspA2I | CCTAGG | 1 cut(s) | 545 |
| AspLEI | GCGC | 6 cut(s) | 92, 231, 268, 368, 382, 552 |
| AspS9I | GGNCC | 2 cut(s) | 440, 1076 |
| AsuC2I | CCSGG | 6 cut(s) | 107, 117, 647, 665, 702, 1080 |
| AsuHPI | GGTGA | 4 cut(s) | 134, 160, 542, 814 |
| AvaII | GGWCC | 2 cut(s) | 440, 1076 |
| AvrII | CCTAGG | 1 cut(s) | 545 |
| BalI | TGGCCA | 2 cut(s) | 156, 308 |
| BanI | GGYRCC | 1 cut(s) | 379 |
| BanII | GRGCYC | 1 cut(s) | 45 |
| BbsI | GAAGAC | 2 cut(s) | 1025, 1106 |
| Bbv12I | GWGCWC | 2 cut(s) | 45, 347 |
| BbvI | GCAGC | 4 cut(s) | 150, 153, 250, 1062 |
| BccI | CCATC | 1 cut(s) | 253 |
| BceAI | ACGGC | 1 cut(s) | 947 |
| BciT130I | CCWGG | 7 cut(s) | 135, 158, 349, 384, 423, 514, 1066 |
| BcnI | CCSGG | 6 cut(s) | 107, 117, 647, 665, 702, 1080 |
| BcoDI | GTCTC | 2 cut(s) | 647, 1150 |
| BfaI | CTAG | 2 cut(s) | 546, 1142 |
| BfmI | CTRYAG | 1 cut(s) | 725 |
| BfoI | RGCGCY | 2 cut(s) | 369, 383 |
| BfuAI | ACCTGC | 1 cut(s) | 556 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 562 |
| BisI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 446, 717, 1051 |
| BlnI | CCTAGG | 1 cut(s) | 545 |
| BlsI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 447, 718, 1052 |
| Bme18I | GGWCC | 2 cut(s) | 440, 1076 |
| BmgT120I | GGNCC | 2 cut(s) | 440, 1076 |
| BmiI | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| BmsI | GCATC | 2 cut(s) | 609, 993 |
| BoxI | GACNNNNGTC | 1 cut(s) | 659 |
| BpiI | GAAGAC | 2 cut(s) | 1025, 1106 |
| BpmI | CTGGAG | 2 cut(s) | 650, 1087 |
| BpuMI | CCSGG | 6 cut(s) | 107, 117, 647, 665, 702, 1080 |
| BsaHI | GRCGYC | 3 cut(s) | 380, 668, 1111 |
| BsaJI | CCNNGG | 6 cut(s) | 105, 250, 383, 513, 545, 1064 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 623, 653, 1060, 1090 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 942 |
| Bse118I | RCCGGY | 3 cut(s) | 368, 587, 1107 |
| Bse1I | ACTGG | 3 cut(s) | 192, 633, 1157 |
| BseBI | CCWGG | 7 cut(s) | 135, 158, 349, 384, 423, 514, 1066 |
| BseDI | CCNNGG | 6 cut(s) | 105, 250, 383, 513, 545, 1064 |
| BseGI | GGATG | 2 cut(s) | 624, 915 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 942 |
| BseMII | CTCAG | 2 cut(s) | 516, 1002 |
| BseNI | ACTGG | 3 cut(s) | 192, 633, 1157 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseXI | GCAGC | 4 cut(s) | 150, 153, 250, 1062 |
| BseYI | CCCAGC | 2 cut(s) | 362, 603 |
| Bsh1236I | CGCG | 2 cut(s) | 74, 90 |
| Bsh1285I | CGRYCG | 3 cut(s) | 78, 393, 940 |
| BshFI | GGCC | 5 cut(s) | 110, 156, 308, 578, 1136 |
| BshNI | GGYRCC | 1 cut(s) | 379 |
| BsiEI | CGRYCG | 3 cut(s) | 78, 393, 940 |
| BsiHKAI | GWGCWC | 2 cut(s) | 45, 347 |
| BsiSI | CCGG | 9 cut(s) | 107, 117, 369, 588, 646, 665, 702, 1079, 1108 |
| BslFI | GGGAC | 2 cut(s) | 453, 647 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 77, 368, 519, 546, 610, 942 |
| BsmAI | GTCTC | 2 cut(s) | 647, 1150 |
| BsmBI | CGTCTC | 1 cut(s) | 647 |
| BsmFI | GGGAC | 2 cut(s) | 453, 647 |
| BsmI | GAATGC | 1 cut(s) | 514 |
| BsnI | GGCC | 5 cut(s) | 110, 156, 308, 578, 1136 |
| Bsp1286I | GDGCHC | 2 cut(s) | 45, 347 |
| BspANI | GGCC | 5 cut(s) | 110, 156, 308, 578, 1136 |
| BspCNI | CTCAG | 2 cut(s) | 517, 1001 |
| BspFNI | CGCG | 2 cut(s) | 74, 90 |
| BspLI | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| BspMAI | CTGCAG | 1 cut(s) | 729 |
| BspMI | ACCTGC | 1 cut(s) | 556 |
| BspPI | GGATC | 9 cut(s) | 168, 201, 527, 637, 782, 961, 986, 1044, 1144 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 1 cut(s) | 379 |
| BsrBI | CCGCTC | 1 cut(s) | 504 |
| BsrFI | RCCGGY | 3 cut(s) | 368, 587, 1107 |
| BsrI | ACTGG | 3 cut(s) | 192, 633, 1157 |
| BssAI | RCCGGY | 3 cut(s) | 368, 587, 1107 |
| BssECI | CCNNGG | 6 cut(s) | 105, 250, 383, 513, 545, 1064 |
| BssNI | GRCGYC | 3 cut(s) | 380, 668, 1111 |
| BssT1I | CCWWGG | 2 cut(s) | 250, 545 |
| Bst2UI | CCWGG | 7 cut(s) | 135, 158, 349, 384, 423, 514, 1066 |
| Bst4CI | ACNGT | 3 cut(s) | 11, 411, 970 |
| Bst6I | CTCTTC | 3 cut(s) | 24, 901, 1026 |
| BstACI | GRCGYC | 3 cut(s) | 380, 668, 1111 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1056 |
| BstC8I | GCNNGC | 7 cut(s) | 36, 332, 370, 554, 589, 729, 1061 |
| BstDEI | CTNAG | 2 cut(s) | 525, 988 |
| BstEII | GGTNACC | 1 cut(s) | 820 |
| BstF5I | GGATG | 2 cut(s) | 624, 915 |
| BstFNI | CGCG | 2 cut(s) | 74, 90 |
| BstH2I | RGCGCY | 2 cut(s) | 369, 383 |
| BstHHI | GCGC | 6 cut(s) | 92, 231, 268, 368, 382, 552 |
| BstMAI | GTCTC | 2 cut(s) | 647, 1150 |
| BstMCI | CGRYCG | 3 cut(s) | 78, 393, 940 |
| BstNI | CCWGG | 7 cut(s) | 135, 158, 349, 384, 423, 514, 1066 |
| BstNSI | RCATGY | 2 cut(s) | 407, 749 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 659 |
| BstPI | GGTNACC | 1 cut(s) | 820 |
| BstSFI | CTRYAG | 1 cut(s) | 725 |
| BstUI | CGCG | 2 cut(s) | 74, 90 |
| BstV1I | GCAGC | 4 cut(s) | 150, 153, 250, 1062 |
| BstV2I | GAAGAC | 2 cut(s) | 1025, 1106 |
| BstX2I | RGATCY | 3 cut(s) | 160, 193, 991 |
| BstYI | RGATCY | 3 cut(s) | 160, 193, 991 |
| BsuRI | GGCC | 5 cut(s) | 110, 156, 308, 578, 1136 |
| BtsCI | GGATG | 2 cut(s) | 624, 915 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| BveI | ACCTGC | 1 cut(s) | 556 |
| Cac8I | GCNNGC | 7 cut(s) | 36, 332, 370, 554, 589, 729, 1061 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1056 |
| CfoI | GCGC | 6 cut(s) | 92, 231, 268, 368, 382, 552 |
| Cfr10I | RCCGGY | 3 cut(s) | 368, 587, 1107 |
| Cfr13I | GGNCC | 2 cut(s) | 440, 1076 |
| CseI | GACGC | 5 cut(s) | 602, 657, 1030, 1042, 1100 |
| Csp6I | GTAC | 3 cut(s) | 175, 395, 407 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 5 cut(s) | 172, 271, 404, 746, 802 |
| CviQI | GTAC | 3 cut(s) | 175, 395, 407 |
| DdeI | CTNAG | 2 cut(s) | 525, 988 |
| DinI | GGCGCC | 1 cut(s) | 381 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 879 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 879 |
| EaeI | YGGCCR | 3 cut(s) | 108, 154, 306 |
| Eam1104I | CTCTTC | 3 cut(s) | 24, 901, 1026 |
| EarI | CTCTTC | 3 cut(s) | 24, 901, 1026 |
| EciI | GGCGGA | 7 cut(s) | 53, 206, 494, 525, 665, 703, 792 |
| Ecl136II | GAGCTC | 1 cut(s) | 43 |
| Eco130I | CCWWGG | 2 cut(s) | 250, 545 |
| Eco24I | GRGCYC | 1 cut(s) | 45 |
| Eco47I | GGWCC | 2 cut(s) | 440, 1076 |
| Eco53kI | GAGCTC | 1 cut(s) | 43 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 264 |
| Eco91I | GGTNACC | 1 cut(s) | 820 |
| EcoICRI | GAGCTC | 1 cut(s) | 43 |
| EcoO65I | GGTNACC | 1 cut(s) | 820 |
| EcoRII | CCWGG | 7 cut(s) | 133, 156, 347, 382, 421, 512, 1064 |
| EcoT14I | CCWWGG | 2 cut(s) | 250, 545 |
| EcoT22I | ATGCAT | 1 cut(s) | 405 |
| EcoT38I | GRGCYC | 1 cut(s) | 45 |
| EgeI | GGCGCC | 1 cut(s) | 381 |
| EheI | GGCGCC | 1 cut(s) | 381 |
| ErhI | CCWWGG | 2 cut(s) | 250, 545 |
| Esp3I | CGTCTC | 1 cut(s) | 647 |
| FaeI | CATG | 5 cut(s) | 175, 274, 407, 749, 805 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 824, 856, 1173, 1205 |
| FaqI | GGGAC | 2 cut(s) | 453, 647 |
| FatI | CATG | 5 cut(s) | 171, 270, 403, 745, 801 |
| FauI | CCCGC | 4 cut(s) | 8, 55, 79, 690 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 446, 717, 1051 |
| FokI | GGATG | 2 cut(s) | 631, 902 |
| FriOI | GRGCYC | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 446, 717, 1051 |
| FspBI | CTAG | 2 cut(s) | 546, 1142 |
| GlaI | GCGC | 6 cut(s) | 91, 230, 267, 367, 381, 551 |
| GluI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 446, 717, 1051 |
| GsaI | CCCAGC | 2 cut(s) | 366, 607 |
| GsuI | CTGGAG | 2 cut(s) | 650, 1087 |
| HaeII | RGCGCY | 2 cut(s) | 369, 383 |
| HaeIII | GGCC | 5 cut(s) | 110, 156, 308, 578, 1136 |
| HapII | CCGG | 9 cut(s) | 107, 117, 369, 588, 646, 665, 702, 1079, 1108 |
| HgaI | GACGC | 5 cut(s) | 602, 657, 1030, 1042, 1100 |
| HhaI | GCGC | 6 cut(s) | 92, 231, 268, 368, 382, 552 |
| Hin1I | GRCGYC | 3 cut(s) | 380, 668, 1111 |
| Hin1II | CATG | 5 cut(s) | 175, 274, 407, 749, 805 |
| Hin6I | GCGC | 6 cut(s) | 90, 229, 266, 366, 380, 550 |
| HinP1I | GCGC | 6 cut(s) | 90, 229, 266, 366, 380, 550 |
| HincII | GTYRAC | 3 cut(s) | 85, 359, 973 |
| HindII | GTYRAC | 3 cut(s) | 85, 359, 973 |
| HinfI | GANTC | 3 cut(s) | 482, 581, 660 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 9 cut(s) | 107, 117, 369, 588, 646, 665, 702, 1079, 1108 |
| HphI | GGTGA | 4 cut(s) | 134, 160, 542, 814 |
| Hpy166II | GTNNAC | 5 cut(s) | 85, 359, 799, 973, 1103 |
| Hpy188I | TCNGA | 9 cut(s) | 67, 678, 737, 857, 885, 937, 991, 1154, 1195 |
| Hpy188III | TCNNGA | 5 cut(s) | 432, 696, 814, 1147, 1206 |
| Hpy8I | GTNNAC | 5 cut(s) | 85, 359, 799, 973, 1103 |
| Hpy99I | CGWCG | 2 cut(s) | 596, 1044 |
| HpyAV | CCTTC | 4 cut(s) | 232, 811, 820, 1172 |
| HpyCH4III | ACNGT | 3 cut(s) | 11, 411, 970 |
| HpyCH4IV | ACGT | 2 cut(s) | 277, 862 |
| HpyCH4V | TGCA | 7 cut(s) | 403, 622, 727, 749, 984, 1050, 1059 |
| HpyF3I | CTNAG | 2 cut(s) | 525, 988 |
| HpySE526I | ACGT | 2 cut(s) | 277, 862 |
| Hsp92I | GRCGYC | 3 cut(s) | 380, 668, 1111 |
| Hsp92II | CATG | 5 cut(s) | 175, 274, 407, 749, 805 |
| HspAI | GCGC | 6 cut(s) | 90, 229, 266, 366, 380, 550 |
| KasI | GGCGCC | 1 cut(s) | 379 |
| KroI | GCCGGC | 2 cut(s) | 368, 587 |
| KroNI | GCCGGC | 2 cut(s) | 370, 589 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 6 cut(s) | 40, 48, 226, 342, 631, 710 |
| Lsp1109I | GCAGC | 4 cut(s) | 150, 153, 250, 1062 |
| LweI | GCATC | 2 cut(s) | 609, 993 |
| MaeI | CTAG | 2 cut(s) | 546, 1142 |
| MaeII | ACGT | 2 cut(s) | 277, 862 |
| MaeIII | GTNAC | 5 cut(s) | 166, 336, 765, 820, 964 |
| MbiI | CCGCTC | 1 cut(s) | 504 |
| MboII | GAAGA | 8 cut(s) | 41, 188, 888, 1027, 1030, 1040, 1043, 1106 |
| MfeI | CAATTG | 1 cut(s) | 1045 |
| MflI | RGATCY | 3 cut(s) | 160, 193, 991 |
| MhlI | GDGCHC | 2 cut(s) | 45, 347 |
| MlsI | TGGCCA | 2 cut(s) | 156, 308 |
| MluCI | AATT | 2 cut(s) | 979, 1045 |
| MluNI | TGGCCA | 2 cut(s) | 156, 308 |
| Mly113I | GGCGCC | 1 cut(s) | 380 |
| MlyI | GAGTC | 1 cut(s) | 669 |
| MmeI | TCCRAC | 3 cut(s) | 90, 931, 1177 |
| Mox20I | TGGCCA | 2 cut(s) | 156, 308 |
| Mph1103I | ATGCAT | 1 cut(s) | 405 |
| MroNI | GCCGGC | 2 cut(s) | 368, 587 |
| MroXI | GAANNNNTTC | 1 cut(s) | 486 |
| MscI | TGGCCA | 2 cut(s) | 156, 308 |
| MseI | TTAA | 4 cut(s) | 84, 842, 929, 978 |
| MslI | CAYNNNNRTG | 1 cut(s) | 302 |
| Msp20I | TGGCCA | 2 cut(s) | 156, 308 |
| MspI | CCGG | 9 cut(s) | 107, 117, 369, 588, 646, 665, 702, 1079, 1108 |
| MunI | CAATTG | 1 cut(s) | 1045 |
| Mva1269I | GAATGC | 1 cut(s) | 514 |
| MvaI | CCWGG | 7 cut(s) | 135, 158, 349, 384, 423, 514, 1066 |
| MvnI | CGCG | 2 cut(s) | 74, 90 |
| NaeI | GCCGGC | 2 cut(s) | 370, 589 |
| NarI | GGCGCC | 1 cut(s) | 380 |
| NciI | CCSGG | 6 cut(s) | 107, 117, 647, 665, 702, 1080 |
| NgoMIV | GCCGGC | 2 cut(s) | 368, 587 |
| NlaIII | CATG | 5 cut(s) | 175, 274, 407, 749, 805 |
| NlaIV | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| NmeAIII | GCCGAG | 1 cut(s) | 541 |
| NmuCI | GTSAC | 5 cut(s) | 166, 336, 765, 820, 964 |
| NsiI | ATGCAT | 1 cut(s) | 405 |
| NspI | RCATGY | 2 cut(s) | 407, 749 |
| OliI | CACNNNNGTG | 1 cut(s) | 302 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 868, 1191 |
| PctI | GAATGC | 1 cut(s) | 514 |
| PdiI | GCCGGC | 2 cut(s) | 370, 589 |
| PdmI | GAANNNNTTC | 1 cut(s) | 486 |
| PfeI | GAWTC | 2 cut(s) | 482, 581 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 447, 718, 1052 |
| Ple19I | CGATCG | 2 cut(s) | 78, 940 |
| PleI | GAGTC | 1 cut(s) | 668 |
| PluTI | GGCGCC | 1 cut(s) | 383 |
| PpsI | GAGTC | 1 cut(s) | 668 |
| PshAI | GACNNNNGTC | 1 cut(s) | 659 |
| Psp124BI | GAGCTC | 1 cut(s) | 45 |
| Psp6I | CCWGG | 7 cut(s) | 133, 156, 347, 382, 421, 512, 1064 |
| PspEI | GGTNACC | 1 cut(s) | 820 |
| PspFI | CCCAGC | 2 cut(s) | 362, 603 |
| PspGI | CCWGG | 7 cut(s) | 133, 156, 347, 382, 421, 512, 1064 |
| PspN4I | GGNNCC | 4 cut(s) | 381, 441, 680, 706 |
| PspPI | GGNCC | 2 cut(s) | 440, 1076 |
| PstI | CTGCAG | 1 cut(s) | 729 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1056 |
| PsuI | RGATCY | 3 cut(s) | 160, 193, 991 |
| PvuI | CGATCG | 2 cut(s) | 78, 940 |
| RsaI | GTAC | 3 cut(s) | 176, 396, 408 |
| RsaNI | GTAC | 3 cut(s) | 175, 395, 407 |
| RseI | CAYNNNNRTG | 1 cut(s) | 302 |
| SacI | GAGCTC | 1 cut(s) | 45 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 4 cut(s) | 84, 842, 929, 978 |
| SatI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 446, 717, 1051 |
| Sau96I | GGNCC | 2 cut(s) | 440, 1076 |
| SbfI | CCTGCAGG | 1 cut(s) | 729 |
| SchI | GAGTC | 1 cut(s) | 669 |
| SdaI | CCTGCAGG | 1 cut(s) | 729 |
| SduI | GDGCHC | 2 cut(s) | 45, 347 |
| SfaNI | GCATC | 2 cut(s) | 609, 993 |
| SfcI | CTRYAG | 1 cut(s) | 725 |
| SfoI | GGCGCC | 1 cut(s) | 381 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1107 |
| SinI | GGWCC | 2 cut(s) | 440, 1076 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 302 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 729 |
| Sse9I | AATT | 2 cut(s) | 979, 1045 |
| SspDI | GGCGCC | 1 cut(s) | 379 |
| SspMI | CTAG | 2 cut(s) | 546, 1142 |
| SstI | GAGCTC | 1 cut(s) | 45 |
| StyI | CCWWGG | 2 cut(s) | 250, 545 |
| TaaI | ACNGT | 3 cut(s) | 11, 411, 970 |
| TaiI | ACGT | 2 cut(s) | 280, 865 |
| TaqI | TCGA | 5 cut(s) | 358, 506, 846, 1013, 1148 |
| TaqII | GACCGA | 2 cut(s) | 379, 754 |
| TasI | AATT | 2 cut(s) | 979, 1045 |
| TauI | GCSGC | 3 cut(s) | 147, 448, 719 |
| TfiI | GAWTC | 2 cut(s) | 482, 581 |
| Tru1I | TTAA | 4 cut(s) | 84, 842, 929, 978 |
| Tru9I | TTAA | 4 cut(s) | 84, 842, 929, 978 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 5 cut(s) | 166, 336, 765, 820, 964 |
| TseI | GCWGC | 4 cut(s) | 138, 141, 263, 1050 |
| Tsp45I | GTSAC | 5 cut(s) | 166, 336, 765, 820, 964 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspGWI | ACGGA | 3 cut(s) | 355, 901, 1145 |
| TspRI | CASTG | 1 cut(s) | 192 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 2 cut(s) | 440, 1076 |
| XceI | RCATGY | 2 cut(s) | 407, 749 |
| XmaJI | CCTAGG | 1 cut(s) | 545 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XmnI | GAANNNNTTC | 1 cut(s) | 486 |
| XspI | CTAG | 2 cut(s) | 546, 1142 |
| Zsp2I | ATGCAT | 1 cut(s) | 405 |