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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 247 |
| Acc36I | ACCTGC | 2 cut(s) | 25, 247 |
| AccB1I | GGYRCC | 2 cut(s) | 524, 532 |
| AccBSI | CCGCTC | 2 cut(s) | 407, 467 |
| AccI | GTMKAC | 1 cut(s) | 39 |
| AccII | CGCG | 2 cut(s) | 90, 266 |
| AclWI | GGATC | 2 cut(s) | 201, 427 |
| AcoI | YGGCCR | 1 cut(s) | 261 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AcyI | GRCGYC | 1 cut(s) | 533 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 73, 256, 416 |
| AgsI | TTSAA | 1 cut(s) | 543 |
| AjnI | CCWGG | 3 cut(s) | 249, 437, 560 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 36, 68 |
| AluBI | AGCT | 2 cut(s) | 181, 292 |
| AluI | AGCT | 2 cut(s) | 181, 292 |
| Alw26I | GTCTC | 2 cut(s) | 119, 452 |
| AlwI | GGATC | 2 cut(s) | 201, 427 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 475 |
| Ama87I | CYCGRG | 2 cut(s) | 370, 510 |
| AoxI | GGCC | 4 cut(s) | 154, 246, 261, 563 |
| ApeKI | GCWGC | 5 cut(s) | 141, 277, 355, 487, 581 |
| AspLEI | GCGC | 3 cut(s) | 92, 282, 535 |
| AspS9I | GGNCC | 3 cut(s) | 247, 271, 305 |
| AsuC2I | CCSGG | 1 cut(s) | 530 |
| AsuHPI | GGTGA | 1 cut(s) | 91 |
| AvaI | CYCGRG | 2 cut(s) | 370, 510 |
| AvaII | GGWCC | 2 cut(s) | 271, 305 |
| BaeGI | GKGCMC | 2 cut(s) | 321, 527 |
| BanI | GGYRCC | 2 cut(s) | 524, 532 |
| BbvI | GCAGC | 5 cut(s) | 153, 264, 342, 499, 568 |
| BciT130I | CCWGG | 3 cut(s) | 251, 439, 562 |
| BciVI | GTATCC | 1 cut(s) | 427 |
| BcnI | CCSGG | 1 cut(s) | 530 |
| BcoDI | GTCTC | 2 cut(s) | 119, 452 |
| BfaI | CTAG | 1 cut(s) | 152 |
| BfmI | CTRYAG | 1 cut(s) | 590 |
| BfoI | RGCGCY | 1 cut(s) | 536 |
| BfuAI | ACCTGC | 2 cut(s) | 25, 247 |
| BfuI | GTATCC | 1 cut(s) | 427 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 7 cut(s) | 142, 145, 264, 278, 356, 488, 582 |
| BlsI | GCNGC | 7 cut(s) | 143, 146, 265, 279, 357, 489, 583 |
| Bme1390I | CCNGG | 4 cut(s) | 251, 439, 530, 562 |
| Bme18I | GGWCC | 2 cut(s) | 271, 305 |
| BmeT110I | CYCGRG | 2 cut(s) | 370, 510 |
| BmgT120I | GGNCC | 3 cut(s) | 247, 271, 305 |
| BmiI | GGNNCC | 3 cut(s) | 273, 526, 534 |
| BmrFI | CCNGG | 4 cut(s) | 251, 439, 530, 562 |
| BmrI | ACTGGG | 2 cut(s) | 111, 129 |
| BmsI | GCATC | 2 cut(s) | 448, 474 |
| BmuI | ACTGGG | 2 cut(s) | 111, 129 |
| Bpu10I | CCTNAGC | 1 cut(s) | 377 |
| BpuMI | CCSGG | 1 cut(s) | 530 |
| BsaHI | GRCGYC | 1 cut(s) | 533 |
| BsaI | GGTCTC | 1 cut(s) | 452 |
| BsaJI | CCNNGG | 4 cut(s) | 243, 249, 390, 528 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 73, 256, 416 |
| Bse118I | RCCGGY | 1 cut(s) | 452 |
| Bse1I | ACTGG | 3 cut(s) | 117, 135, 480 |
| BseBI | CCWGG | 3 cut(s) | 251, 439, 562 |
| BseDI | CCNNGG | 4 cut(s) | 243, 249, 390, 528 |
| BseGI | GGATG | 1 cut(s) | 489 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 73, 256, 416 |
| BseMII | CTCAG | 1 cut(s) | 482 |
| BseNI | ACTGG | 3 cut(s) | 117, 135, 480 |
| BseSI | GKGCMC | 2 cut(s) | 321, 527 |
| BseXI | GCAGC | 5 cut(s) | 153, 264, 342, 499, 568 |
| BseYI | CCCAGC | 4 cut(s) | 70, 106, 321, 442 |
| Bsh1236I | CGCG | 2 cut(s) | 90, 266 |
| BshFI | GGCC | 4 cut(s) | 156, 248, 263, 565 |
| BshNI | GGYRCC | 2 cut(s) | 524, 532 |
| BsiHKCI | CYCGRG | 2 cut(s) | 370, 510 |
| BsiSI | CCGG | 2 cut(s) | 453, 530 |
| BslFI | GGGAC | 1 cut(s) | 257 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 73, 256, 416 |
| BsmAI | GTCTC | 2 cut(s) | 119, 452 |
| BsmFI | GGGAC | 1 cut(s) | 257 |
| BsmI | GAATGC | 1 cut(s) | 358 |
| BsnI | GGCC | 4 cut(s) | 156, 248, 263, 565 |
| Bso31I | GGTCTC | 1 cut(s) | 452 |
| BsoBI | CYCGRG | 2 cut(s) | 370, 510 |
| Bsp1286I | GDGCHC | 2 cut(s) | 321, 527 |
| Bsp143I | GATC | 2 cut(s) | 193, 419 |
| BspANI | GGCC | 4 cut(s) | 156, 248, 263, 565 |
| BspCNI | CTCAG | 1 cut(s) | 481 |
| BspFNI | CGCG | 2 cut(s) | 90, 266 |
| BspLI | GGNNCC | 3 cut(s) | 273, 526, 534 |
| BspMAI | CTGCAG | 1 cut(s) | 594 |
| BspMI | ACCTGC | 2 cut(s) | 25, 247 |
| BspPI | GGATC | 2 cut(s) | 201, 427 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BspT107I | GGYRCC | 2 cut(s) | 524, 532 |
| BspTNI | GGTCTC | 1 cut(s) | 452 |
| BsrBI | CCGCTC | 2 cut(s) | 407, 467 |
| BsrFI | RCCGGY | 1 cut(s) | 452 |
| BsrI | ACTGG | 3 cut(s) | 117, 135, 480 |
| BssAI | RCCGGY | 1 cut(s) | 452 |
| BssECI | CCNNGG | 4 cut(s) | 243, 249, 390, 528 |
| BssMI | GATC | 2 cut(s) | 193, 419 |
| BssNI | GRCGYC | 1 cut(s) | 533 |
| Bst2UI | CCWGG | 3 cut(s) | 251, 439, 562 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 226 |
| BstACI | GRCGYC | 1 cut(s) | 533 |
| BstC8I | GCNNGC | 3 cut(s) | 149, 454, 594 |
| BstDEI | CTNAG | 2 cut(s) | 377, 468 |
| BstF5I | GGATG | 1 cut(s) | 489 |
| BstFNI | CGCG | 2 cut(s) | 90, 266 |
| BstH2I | RGCGCY | 1 cut(s) | 536 |
| BstHHI | GCGC | 3 cut(s) | 92, 282, 535 |
| BstKTI | GATC | 2 cut(s) | 196, 422 |
| BstMAI | GTCTC | 2 cut(s) | 119, 452 |
| BstMBI | GATC | 2 cut(s) | 193, 419 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 153, 316, 458, 496 |
| BstNI | CCWGG | 3 cut(s) | 251, 439, 562 |
| BstSCI | CCNGG | 4 cut(s) | 249, 437, 528, 560 |
| BstSFI | CTRYAG | 1 cut(s) | 590 |
| BstSLI | GKGCMC | 2 cut(s) | 321, 527 |
| BstUI | CGCG | 2 cut(s) | 90, 266 |
| BstV1I | GCAGC | 5 cut(s) | 153, 264, 342, 499, 568 |
| BstX2I | RGATCY | 1 cut(s) | 193 |
| BstYI | RGATCY | 1 cut(s) | 193 |
| BsuI | GTATCC | 1 cut(s) | 427 |
| BsuRI | GGCC | 4 cut(s) | 156, 248, 263, 565 |
| BtgZI | GCGATG | 2 cut(s) | 336, 471 |
| BtsCI | GGATG | 1 cut(s) | 489 |
| BtsI | GCAGTG | 1 cut(s) | 576 |
| BtsIMutI | CAGTG | 2 cut(s) | 124, 576 |
| BveI | ACCTGC | 2 cut(s) | 25, 247 |
| Cac8I | GCNNGC | 3 cut(s) | 149, 454, 594 |
| CaiI | CAGNNNCTG | 1 cut(s) | 475 |
| CfoI | GCGC | 3 cut(s) | 92, 282, 535 |
| Cfr10I | RCCGGY | 1 cut(s) | 452 |
| Cfr13I | GGNCC | 3 cut(s) | 247, 271, 305 |
| CseI | GACGC | 1 cut(s) | 546 |
| CviAII | CATG | 1 cut(s) | 338 |
| DdeI | CTNAG | 2 cut(s) | 377, 468 |
| DinI | GGCGCC | 1 cut(s) | 534 |
| DpnI | GATC | 2 cut(s) | 195, 421 |
| DpnII | GATC | 2 cut(s) | 193, 419 |
| EaeI | YGGCCR | 1 cut(s) | 261 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 226 |
| EarI | CTCTTC | 2 cut(s) | 24, 226 |
| EciI | GGCGGA | 1 cut(s) | 206 |
| Eco31I | GGTCTC | 1 cut(s) | 452 |
| Eco47I | GGWCC | 2 cut(s) | 271, 305 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| Eco88I | CYCGRG | 2 cut(s) | 370, 510 |
| EcoRII | CCWGG | 3 cut(s) | 249, 437, 560 |
| EgeI | GGCGCC | 1 cut(s) | 534 |
| EheI | GGCGCC | 1 cut(s) | 534 |
| FaeI | CATG | 1 cut(s) | 341 |
| FaiI | YATR | 3 cut(s) | 175, 177, 339 |
| FaqI | GGGAC | 1 cut(s) | 257 |
| FatI | CATG | 1 cut(s) | 337 |
| FauI | CCCGC | 5 cut(s) | 8, 156, 282, 412, 423 |
| FblI | GTMKAC | 1 cut(s) | 39 |
| Fnu4HI | GCNGC | 7 cut(s) | 142, 145, 264, 278, 356, 488, 582 |
| FokI | GGATG | 1 cut(s) | 496 |
| Fsp4HI | GCNGC | 7 cut(s) | 142, 145, 264, 278, 356, 488, 582 |
| FspBI | CTAG | 1 cut(s) | 152 |
| GlaI | GCGC | 3 cut(s) | 91, 281, 534 |
| GluI | GCNGC | 7 cut(s) | 142, 145, 264, 278, 356, 488, 582 |
| GsaI | CCCAGC | 4 cut(s) | 74, 110, 325, 446 |
| HaeII | RGCGCY | 1 cut(s) | 536 |
| HaeIII | GGCC | 4 cut(s) | 156, 248, 263, 565 |
| HapII | CCGG | 2 cut(s) | 453, 530 |
| HgaI | GACGC | 1 cut(s) | 546 |
| HhaI | GCGC | 3 cut(s) | 92, 282, 535 |
| Hin1I | GRCGYC | 1 cut(s) | 533 |
| Hin1II | CATG | 1 cut(s) | 341 |
| Hin6I | GCGC | 3 cut(s) | 90, 280, 533 |
| HinP1I | GCGC | 3 cut(s) | 90, 280, 533 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HinfI | GANTC | 3 cut(s) | 347, 518, 586 |
| HpaII | CCGG | 2 cut(s) | 453, 530 |
| HphI | GGTGA | 1 cut(s) | 91 |
| Hpy166II | GTNNAC | 3 cut(s) | 40, 85, 221 |
| Hpy188I | TCNGA | 2 cut(s) | 471, 602 |
| Hpy8I | GTNNAC | 3 cut(s) | 40, 85, 221 |
| HpyCH4V | TGCA | 4 cut(s) | 358, 461, 487, 592 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 153, 316, 458, 496 |
| HpyF3I | CTNAG | 2 cut(s) | 377, 468 |
| Hsp92I | GRCGYC | 1 cut(s) | 533 |
| Hsp92II | CATG | 1 cut(s) | 341 |
| HspAI | GCGC | 3 cut(s) | 90, 280, 533 |
| KasI | GGCGCC | 1 cut(s) | 532 |
| KroI | GCCGGC | 1 cut(s) | 452 |
| KroNI | GCCGGC | 1 cut(s) | 454 |
| Kzo9I | GATC | 2 cut(s) | 193, 419 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 3 cut(s) | 253, 412, 496 |
| Lsp1109I | GCAGC | 5 cut(s) | 153, 264, 342, 499, 568 |
| LweI | GCATC | 2 cut(s) | 448, 474 |
| MaeI | CTAG | 1 cut(s) | 152 |
| MalI | GATC | 2 cut(s) | 195, 421 |
| MbiI | CCGCTC | 2 cut(s) | 407, 467 |
| MboI | GATC | 2 cut(s) | 193, 419 |
| MboII | GAAGA | 3 cut(s) | 41, 188, 243 |
| MflI | RGATCY | 1 cut(s) | 193 |
| MhlI | GDGCHC | 2 cut(s) | 321, 527 |
| MluCI | AATT | 2 cut(s) | 300, 537 |
| Mly113I | GGCGCC | 1 cut(s) | 533 |
| MlyI | GAGTC | 2 cut(s) | 356, 527 |
| MmeI | TCCRAC | 2 cut(s) | 312, 474 |
| MnlI | CCTC | 7 cut(s) | 173, 178, 238, 334, 366, 385, 410 |
| MreI | CGCCGGCG | 1 cut(s) | 452 |
| MroNI | GCCGGC | 1 cut(s) | 452 |
| MseI | TTAA | 1 cut(s) | 506 |
| MspA1I | CMGCKG | 3 cut(s) | 74, 277, 430 |
| MspI | CCGG | 2 cut(s) | 453, 530 |
| MspR9I | CCNGG | 4 cut(s) | 251, 439, 530, 562 |
| Mva1269I | GAATGC | 1 cut(s) | 358 |
| MvaI | CCWGG | 3 cut(s) | 251, 439, 562 |
| MvnI | CGCG | 2 cut(s) | 90, 266 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 153, 316, 458, 496 |
| NaeI | GCCGGC | 1 cut(s) | 454 |
| NarI | GGCGCC | 1 cut(s) | 533 |
| NciI | CCSGG | 1 cut(s) | 530 |
| NdeII | GATC | 2 cut(s) | 193, 419 |
| NgoMIV | GCCGGC | 1 cut(s) | 452 |
| NlaIII | CATG | 1 cut(s) | 341 |
| NlaIV | GGNNCC | 3 cut(s) | 273, 526, 534 |
| PaeR7I | CTCGAG | 1 cut(s) | 370 |
| PaqCI | CACCTGC | 1 cut(s) | 247 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PctI | GAATGC | 1 cut(s) | 358 |
| PdiI | GCCGGC | 1 cut(s) | 454 |
| PfeI | GAWTC | 1 cut(s) | 586 |
| PkrI | GCNGC | 7 cut(s) | 143, 146, 265, 279, 357, 489, 583 |
| PleI | GAGTC | 2 cut(s) | 355, 526 |
| PluTI | GGCGCC | 1 cut(s) | 536 |
| PpsI | GAGTC | 2 cut(s) | 355, 526 |
| Psp6I | CCWGG | 3 cut(s) | 249, 437, 560 |
| PspFI | CCCAGC | 4 cut(s) | 70, 106, 321, 442 |
| PspGI | CCWGG | 3 cut(s) | 249, 437, 560 |
| PspN4I | GGNNCC | 3 cut(s) | 273, 526, 534 |
| PspPI | GGNCC | 3 cut(s) | 247, 271, 305 |
| PspXI | VCTCGAGB | 1 cut(s) | 370 |
| PstI | CTGCAG | 1 cut(s) | 594 |
| PstNI | CAGNNNCTG | 1 cut(s) | 475 |
| PsuI | RGATCY | 1 cut(s) | 193 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 506 |
| SatI | GCNGC | 7 cut(s) | 142, 145, 264, 278, 356, 488, 582 |
| Sau3AI | GATC | 2 cut(s) | 193, 419 |
| Sau96I | GGNCC | 3 cut(s) | 247, 271, 305 |
| SchI | GAGTC | 2 cut(s) | 356, 527 |
| ScrFI | CCNGG | 4 cut(s) | 251, 439, 530, 562 |
| SduI | GDGCHC | 2 cut(s) | 321, 527 |
| SetI | ASST | 6 cut(s) | 39, 165, 183, 261, 294, 345 |
| SfaNI | GCATC | 2 cut(s) | 448, 474 |
| SfcI | CTRYAG | 1 cut(s) | 590 |
| SfoI | GGCGCC | 1 cut(s) | 534 |
| Sfr274I | CTCGAG | 1 cut(s) | 370 |
| SgrAI | CRCCGGYG | 1 cut(s) | 452 |
| SinI | GGWCC | 2 cut(s) | 271, 305 |
| SlaI | CTCGAG | 1 cut(s) | 370 |
| SmlI | CTYRAG | 1 cut(s) | 370 |
| SmoI | CTYRAG | 1 cut(s) | 370 |
| Sse9I | AATT | 2 cut(s) | 300, 537 |
| SspDI | GGCGCC | 1 cut(s) | 532 |
| SspMI | CTAG | 1 cut(s) | 152 |
| StyD4I | CCNGG | 4 cut(s) | 249, 437, 528, 560 |
| TaqI | TCGA | 1 cut(s) | 371 |
| TaqII | GACCGA | 1 cut(s) | 257 |
| TasI | AATT | 2 cut(s) | 300, 537 |
| TauI | GCSGC | 2 cut(s) | 147, 266 |
| TfiI | GAWTC | 1 cut(s) | 586 |
| Tru1I | TTAA | 1 cut(s) | 506 |
| Tru9I | TTAA | 1 cut(s) | 506 |
| TscAI | CASTG | 2 cut(s) | 124, 583 |
| TseI | GCWGC | 5 cut(s) | 141, 277, 355, 487, 581 |
| TspGWI | ACGGA | 1 cut(s) | 496 |
| TspRI | CASTG | 2 cut(s) | 124, 583 |
| VpaK11BI | GGWCC | 2 cut(s) | 271, 305 |
| XhoI | CTCGAG | 1 cut(s) | 370 |
| XmiI | GTMKAC | 1 cut(s) | 39 |
| XspI | CTAG | 1 cut(s) | 152 |