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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 139 |
| AccB1I | GGYRCC | 2 cut(s) | 139, 433 |
| AccBSI | CCGCTC | 2 cut(s) | 278, 376 |
| AccII | CGCG | 1 cut(s) | 51 |
| AclWI | GGATC | 3 cut(s) | 174, 336, 556 |
| AcoI | YGGCCR | 1 cut(s) | 35 |
| AdeI | CACNNNGTG | 1 cut(s) | 629 |
| AfaI | GTAC | 3 cut(s) | 141, 155, 170 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 142, 320, 323, 324, 325, 476 |
| AgsI | TTSAA | 2 cut(s) | 452, 588 |
| AjnI | CCWGG | 5 cut(s) | 135, 319, 346, 469, 474 |
| AleI | CACNNNNGTG | 1 cut(s) | 43 |
| AluBI | AGCT | 3 cut(s) | 63, 201, 505 |
| AluI | AGCT | 3 cut(s) | 63, 201, 505 |
| Alw21I | GWGCWC | 1 cut(s) | 121 |
| Alw26I | GTCTC | 1 cut(s) | 361 |
| AlwI | GGATC | 3 cut(s) | 174, 336, 556 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 384 |
| Ama87I | CYCGRG | 2 cut(s) | 279, 419 |
| AoxI | GGCC | 6 cut(s) | 14, 20, 35, 283, 472, 478 |
| ApeKI | GCWGC | 3 cut(s) | 186, 396, 490 |
| Asp718I | GGTACC | 1 cut(s) | 139 |
| AspA2I | CCTAGG | 1 cut(s) | 286 |
| AspLEI | GCGC | 2 cut(s) | 53, 191 |
| AspS9I | GGNCC | 3 cut(s) | 21, 214, 478 |
| AsuC2I | CCSGG | 1 cut(s) | 94 |
| AsuHPI | GGTGA | 2 cut(s) | 75, 588 |
| AsuNHI | GCTAGC | 1 cut(s) | 201 |
| AvaI | CYCGRG | 2 cut(s) | 279, 419 |
| AvaII | GGWCC | 1 cut(s) | 214 |
| AvrII | CCTAGG | 1 cut(s) | 286 |
| BaeGI | GKGCMC | 1 cut(s) | 230 |
| BalI | TGGCCA | 1 cut(s) | 37 |
| BanI | GGYRCC | 2 cut(s) | 139, 433 |
| BbsI | GAAGAC | 1 cut(s) | 562 |
| Bbv12I | GWGCWC | 1 cut(s) | 121 |
| BbvI | GCAGC | 3 cut(s) | 173, 408, 477 |
| BciT130I | CCWGG | 5 cut(s) | 137, 321, 348, 471, 476 |
| BciVI | GTATCC | 1 cut(s) | 336 |
| BcnI | CCSGG | 1 cut(s) | 94 |
| BcoDI | GTCTC | 1 cut(s) | 361 |
| BfaI | CTAG | 2 cut(s) | 202, 287 |
| BfuI | GTATCC | 1 cut(s) | 336 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 94 |
| BisI | GCNGC | 3 cut(s) | 187, 397, 491 |
| BlnI | CCTAGG | 1 cut(s) | 286 |
| BlsI | GCNGC | 3 cut(s) | 188, 398, 492 |
| Bme1390I | CCNGG | 6 cut(s) | 94, 137, 321, 348, 471, 476 |
| Bme18I | GGWCC | 1 cut(s) | 214 |
| BmeT110I | CYCGRG | 2 cut(s) | 279, 419 |
| BmgT120I | GGNCC | 3 cut(s) | 21, 214, 478 |
| BmiI | GGNNCC | 2 cut(s) | 141, 435 |
| BmrFI | CCNGG | 6 cut(s) | 94, 137, 321, 348, 471, 476 |
| BmsI | GCATC | 4 cut(s) | 357, 383, 475, 609 |
| BmtI | GCTAGC | 1 cut(s) | 205 |
| BpiI | GAAGAC | 1 cut(s) | 562 |
| BpmI | CTGGAG | 1 cut(s) | 97 |
| BpuMI | CCSGG | 1 cut(s) | 94 |
| BsaI | GGTCTC | 1 cut(s) | 361 |
| BsaJI | CCNNGG | 5 cut(s) | 17, 286, 299, 319, 475 |
| BsaWI | WCCGGW | 1 cut(s) | 142 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 137, 167 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 142, 320, 323, 324, 325, 476 |
| Bse118I | RCCGGY | 2 cut(s) | 361, 513 |
| Bse1I | ACTGG | 2 cut(s) | 163, 389 |
| BseBI | CCWGG | 5 cut(s) | 137, 321, 348, 471, 476 |
| BseDI | CCNNGG | 5 cut(s) | 17, 286, 299, 319, 475 |
| BseGI | GGATG | 1 cut(s) | 466 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 142, 320, 323, 324, 325, 476 |
| BseMII | CTCAG | 1 cut(s) | 391 |
| BseNI | ACTGG | 2 cut(s) | 163, 389 |
| BseSI | GKGCMC | 1 cut(s) | 230 |
| BseXI | GCAGC | 3 cut(s) | 173, 408, 477 |
| BseYI | CCCAGC | 2 cut(s) | 230, 351 |
| Bsh1236I | CGCG | 1 cut(s) | 51 |
| BshFI | GGCC | 6 cut(s) | 16, 22, 37, 285, 474, 480 |
| BshNI | GGYRCC | 2 cut(s) | 139, 433 |
| BsiHKAI | GWGCWC | 1 cut(s) | 121 |
| BsiHKCI | CYCGRG | 2 cut(s) | 279, 419 |
| BsiSI | CCGG | 4 cut(s) | 94, 143, 362, 514 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 142, 320, 323, 324, 325, 476 |
| BsmAI | GTCTC | 1 cut(s) | 361 |
| BsnI | GGCC | 6 cut(s) | 16, 22, 37, 285, 474, 480 |
| Bso31I | GGTCTC | 1 cut(s) | 361 |
| BsoBI | CYCGRG | 2 cut(s) | 279, 419 |
| Bsp1286I | GDGCHC | 2 cut(s) | 121, 230 |
| Bsp143I | GATC | 3 cut(s) | 179, 328, 561 |
| BspANI | GGCC | 6 cut(s) | 16, 22, 37, 285, 474, 480 |
| BspCNI | CTCAG | 1 cut(s) | 390 |
| BspFNI | CGCG | 1 cut(s) | 51 |
| BspLI | GGNNCC | 2 cut(s) | 141, 435 |
| BspOI | GCTAGC | 1 cut(s) | 205 |
| BspPI | GGATC | 3 cut(s) | 174, 336, 556 |
| BspT107I | GGYRCC | 2 cut(s) | 139, 433 |
| BspTNI | GGTCTC | 1 cut(s) | 361 |
| BsrBI | CCGCTC | 2 cut(s) | 278, 376 |
| BsrFI | RCCGGY | 2 cut(s) | 361, 513 |
| BsrI | ACTGG | 2 cut(s) | 163, 389 |
| BssAI | RCCGGY | 2 cut(s) | 361, 513 |
| BssECI | CCNNGG | 5 cut(s) | 17, 286, 299, 319, 475 |
| BssMI | GATC | 3 cut(s) | 179, 328, 561 |
| BssT1I | CCWWGG | 1 cut(s) | 286 |
| Bst2UI | CCWGG | 5 cut(s) | 137, 321, 348, 471, 476 |
| Bst4CI | ACNGT | 1 cut(s) | 110 |
| BstC8I | GCNNGC | 3 cut(s) | 203, 363, 503 |
| BstDEI | CTNAG | 1 cut(s) | 377 |
| BstEII | GGTNACC | 2 cut(s) | 81, 594 |
| BstF5I | GGATG | 1 cut(s) | 466 |
| BstFNI | CGCG | 1 cut(s) | 51 |
| BstHHI | GCGC | 2 cut(s) | 53, 191 |
| BstKTI | GATC | 3 cut(s) | 182, 331, 564 |
| BstMAI | GTCTC | 1 cut(s) | 361 |
| BstMBI | GATC | 3 cut(s) | 179, 328, 561 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 48, 94, 183, 225, 367, 405 |
| BstNI | CCWGG | 5 cut(s) | 137, 321, 348, 471, 476 |
| BstNSI | RCATGY | 1 cut(s) | 177 |
| BstPI | GGTNACC | 2 cut(s) | 81, 594 |
| BstSCI | CCNGG | 6 cut(s) | 92, 135, 319, 346, 469, 474 |
| BstSLI | GKGCMC | 1 cut(s) | 230 |
| BstUI | CGCG | 1 cut(s) | 51 |
| BstV1I | GCAGC | 3 cut(s) | 173, 408, 477 |
| BstV2I | GAAGAC | 1 cut(s) | 562 |
| BstX2I | RGATCY | 1 cut(s) | 179 |
| BstYI | RGATCY | 1 cut(s) | 179 |
| BsuI | GTATCC | 1 cut(s) | 336 |
| BsuRI | GGCC | 6 cut(s) | 16, 22, 37, 285, 474, 480 |
| BtgZI | GCGATG | 1 cut(s) | 380 |
| BtsCI | GGATG | 1 cut(s) | 466 |
| BtsI | GCAGTG | 1 cut(s) | 485 |
| BtsIMutI | CAGTG | 1 cut(s) | 485 |
| Cac8I | GCNNGC | 3 cut(s) | 203, 363, 503 |
| CaiI | CAGNNNCTG | 1 cut(s) | 384 |
| CfoI | GCGC | 2 cut(s) | 53, 191 |
| Cfr10I | RCCGGY | 2 cut(s) | 361, 513 |
| Cfr13I | GGNCC | 3 cut(s) | 21, 214, 478 |
| CsiI | ACCWGGT | 1 cut(s) | 135 |
| Csp6I | GTAC | 3 cut(s) | 140, 154, 169 |
| CviAII | CATG | 2 cut(s) | 174, 576 |
| CviQI | GTAC | 3 cut(s) | 140, 154, 169 |
| DdeI | CTNAG | 1 cut(s) | 377 |
| DpnI | GATC | 3 cut(s) | 181, 330, 563 |
| DpnII | GATC | 3 cut(s) | 179, 328, 561 |
| DraIII | CACNNNGTG | 1 cut(s) | 629 |
| EaeI | YGGCCR | 1 cut(s) | 35 |
| Eco130I | CCWWGG | 1 cut(s) | 286 |
| Eco147I | AGGCCT | 1 cut(s) | 285 |
| Eco31I | GGTCTC | 1 cut(s) | 361 |
| Eco47I | GGWCC | 1 cut(s) | 214 |
| Eco88I | CYCGRG | 2 cut(s) | 279, 419 |
| Eco91I | GGTNACC | 2 cut(s) | 81, 594 |
| EcoO65I | GGTNACC | 2 cut(s) | 81, 594 |
| EcoRII | CCWGG | 5 cut(s) | 135, 319, 346, 469, 474 |
| EcoT14I | CCWWGG | 1 cut(s) | 286 |
| ErhI | CCWWGG | 1 cut(s) | 286 |
| FaeI | CATG | 2 cut(s) | 177, 579 |
| FaiI | YATR | 5 cut(s) | 128, 175, 519, 521, 577 |
| FatI | CATG | 2 cut(s) | 173, 575 |
| FauI | CCCGC | 6 cut(s) | 191, 198, 317, 321, 332, 544 |
| FauNDI | CATATG | 1 cut(s) | 519 |
| Fnu4HI | GCNGC | 3 cut(s) | 187, 397, 491 |
| FokI | GGATG | 1 cut(s) | 453 |
| Fsp4HI | GCNGC | 3 cut(s) | 187, 397, 491 |
| FspBI | CTAG | 2 cut(s) | 202, 287 |
| GlaI | GCGC | 2 cut(s) | 52, 190 |
| GluI | GCNGC | 3 cut(s) | 187, 397, 491 |
| GsaI | CCCAGC | 2 cut(s) | 234, 355 |
| GsuI | CTGGAG | 1 cut(s) | 97 |
| HaeIII | GGCC | 6 cut(s) | 16, 22, 37, 285, 474, 480 |
| HapII | CCGG | 4 cut(s) | 94, 143, 362, 514 |
| HhaI | GCGC | 2 cut(s) | 53, 191 |
| Hin1II | CATG | 2 cut(s) | 177, 579 |
| Hin6I | GCGC | 2 cut(s) | 51, 189 |
| HinP1I | GCGC | 2 cut(s) | 51, 189 |
| HinfI | GANTC | 4 cut(s) | 256, 495, 528, 611 |
| HpaII | CCGG | 4 cut(s) | 94, 143, 362, 514 |
| HphI | GGTGA | 2 cut(s) | 75, 588 |
| Hpy166II | GTNNAC | 2 cut(s) | 171, 573 |
| Hpy188I | TCNGA | 2 cut(s) | 380, 511 |
| Hpy188III | TCNNGA | 1 cut(s) | 588 |
| Hpy8I | GTNNAC | 2 cut(s) | 171, 573 |
| HpyAV | CCTTC | 4 cut(s) | 243, 576, 585, 594 |
| HpyCH4III | ACNGT | 1 cut(s) | 110 |
| HpyCH4V | TGCA | 6 cut(s) | 177, 370, 396, 466, 501, 523 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 48, 94, 183, 225, 367, 405 |
| HpyF3I | CTNAG | 1 cut(s) | 377 |
| Hsp92II | CATG | 2 cut(s) | 177, 579 |
| HspAI | GCGC | 2 cut(s) | 51, 189 |
| KpnI | GGTACC | 1 cut(s) | 143 |
| KroI | GCCGGC | 1 cut(s) | 361 |
| KroNI | GCCGGC | 1 cut(s) | 363 |
| Kzo9I | GATC | 3 cut(s) | 179, 328, 561 |
| LmnI | GCTCC | 3 cut(s) | 116, 145, 405 |
| Lsp1109I | GCAGC | 3 cut(s) | 173, 408, 477 |
| LweI | GCATC | 4 cut(s) | 357, 383, 475, 609 |
| MabI | ACCWGGT | 1 cut(s) | 135 |
| MaeI | CTAG | 2 cut(s) | 202, 287 |
| MaeIII | GTNAC | 4 cut(s) | 81, 110, 539, 594 |
| MalI | GATC | 3 cut(s) | 181, 330, 563 |
| MbiI | CCGCTC | 2 cut(s) | 278, 376 |
| MboI | GATC | 3 cut(s) | 179, 328, 561 |
| MboII | GAAGA | 1 cut(s) | 567 |
| MflI | RGATCY | 1 cut(s) | 179 |
| MhlI | GDGCHC | 2 cut(s) | 121, 230 |
| MlsI | TGGCCA | 1 cut(s) | 37 |
| MluCI | AATT | 3 cut(s) | 209, 332, 446 |
| MluNI | TGGCCA | 1 cut(s) | 37 |
| MlyI | GAGTC | 2 cut(s) | 265, 522 |
| MmeI | TCCRAC | 2 cut(s) | 221, 383 |
| MnlI | CCTC | 5 cut(s) | 12, 72, 230, 275, 294 |
| Mox20I | TGGCCA | 1 cut(s) | 37 |
| MreI | CGCCGGCG | 1 cut(s) | 361 |
| MroNI | GCCGGC | 1 cut(s) | 361 |
| MscI | TGGCCA | 1 cut(s) | 37 |
| MslI | CAYNNNNRTG | 1 cut(s) | 43 |
| Msp20I | TGGCCA | 1 cut(s) | 37 |
| MspA1I | CMGCKG | 3 cut(s) | 186, 339, 551 |
| MspI | CCGG | 4 cut(s) | 94, 143, 362, 514 |
| MspR9I | CCNGG | 6 cut(s) | 94, 137, 321, 348, 471, 476 |
| MvaI | CCWGG | 5 cut(s) | 137, 321, 348, 471, 476 |
| MvnI | CGCG | 1 cut(s) | 51 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 48, 94, 183, 225, 367, 405 |
| NaeI | GCCGGC | 1 cut(s) | 363 |
| NciI | CCSGG | 1 cut(s) | 94 |
| NdeI | CATATG | 1 cut(s) | 519 |
| NdeII | GATC | 3 cut(s) | 179, 328, 561 |
| NgoMIV | GCCGGC | 1 cut(s) | 361 |
| NheI | GCTAGC | 1 cut(s) | 201 |
| NlaIII | CATG | 2 cut(s) | 177, 579 |
| NlaIV | GGNNCC | 2 cut(s) | 141, 435 |
| NmeAIII | GCCGAG | 1 cut(s) | 42 |
| NmuCI | GTSAC | 4 cut(s) | 81, 110, 539, 594 |
| NspI | RCATGY | 1 cut(s) | 177 |
| OliI | CACNNNNGTG | 1 cut(s) | 43 |
| PaeR7I | CTCGAG | 1 cut(s) | 279 |
| PceI | AGGCCT | 1 cut(s) | 285 |
| PdiI | GCCGGC | 1 cut(s) | 363 |
| PfeI | GAWTC | 2 cut(s) | 495, 611 |
| PkrI | GCNGC | 3 cut(s) | 188, 398, 492 |
| PleI | GAGTC | 2 cut(s) | 264, 522 |
| PpsI | GAGTC | 2 cut(s) | 264, 522 |
| Psp6I | CCWGG | 5 cut(s) | 135, 319, 346, 469, 474 |
| PspEI | GGTNACC | 2 cut(s) | 81, 594 |
| PspFI | CCCAGC | 2 cut(s) | 230, 351 |
| PspGI | CCWGG | 5 cut(s) | 135, 319, 346, 469, 474 |
| PspN4I | GGNNCC | 2 cut(s) | 141, 435 |
| PspPI | GGNCC | 3 cut(s) | 21, 214, 478 |
| PspXI | VCTCGAGB | 1 cut(s) | 279 |
| PstNI | CAGNNNCTG | 1 cut(s) | 384 |
| PsuI | RGATCY | 1 cut(s) | 179 |
| RsaI | GTAC | 3 cut(s) | 141, 155, 170 |
| RsaNI | GTAC | 3 cut(s) | 140, 154, 169 |
| RseI | CAYNNNNRTG | 1 cut(s) | 43 |
| SatI | GCNGC | 3 cut(s) | 187, 397, 491 |
| Sau3AI | GATC | 3 cut(s) | 179, 328, 561 |
| Sau96I | GGNCC | 3 cut(s) | 21, 214, 478 |
| SchI | GAGTC | 2 cut(s) | 265, 522 |
| ScrFI | CCNGG | 6 cut(s) | 94, 137, 321, 348, 471, 476 |
| SduI | GDGCHC | 2 cut(s) | 121, 230 |
| SetI | ASST | 8 cut(s) | 65, 83, 138, 203, 349, 507, 586, 596 |
| SexAI | ACCWGGT | 1 cut(s) | 135 |
| SfaNI | GCATC | 4 cut(s) | 357, 383, 475, 609 |
| Sfr274I | CTCGAG | 1 cut(s) | 279 |
| SgrAI | CRCCGGYG | 1 cut(s) | 361 |
| SinI | GGWCC | 1 cut(s) | 214 |
| SlaI | CTCGAG | 1 cut(s) | 279 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 43 |
| SmlI | CTYRAG | 1 cut(s) | 279 |
| SmoI | CTYRAG | 1 cut(s) | 279 |
| Sse9I | AATT | 3 cut(s) | 209, 332, 446 |
| SseBI | AGGCCT | 1 cut(s) | 285 |
| SspMI | CTAG | 2 cut(s) | 202, 287 |
| StuI | AGGCCT | 1 cut(s) | 285 |
| StyD4I | CCNGG | 6 cut(s) | 92, 135, 319, 346, 469, 474 |
| StyI | CCWWGG | 1 cut(s) | 286 |
| TaaI | ACNGT | 1 cut(s) | 110 |
| TaqI | TCGA | 2 cut(s) | 280, 614 |
| TasI | AATT | 3 cut(s) | 209, 332, 446 |
| TatI | WGTACW | 1 cut(s) | 168 |
| TfiI | GAWTC | 2 cut(s) | 495, 611 |
| TscAI | CASTG | 1 cut(s) | 492 |
| TseFI | GTSAC | 4 cut(s) | 81, 110, 539, 594 |
| TseI | GCWGC | 3 cut(s) | 186, 396, 490 |
| Tsp45I | GTSAC | 4 cut(s) | 81, 110, 539, 594 |
| TspGWI | ACGGA | 1 cut(s) | 129 |
| TspRI | CASTG | 1 cut(s) | 492 |
| VpaK11BI | GGWCC | 1 cut(s) | 214 |
| XceI | RCATGY | 1 cut(s) | 177 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 317 |
| XhoI | CTCGAG | 1 cut(s) | 279 |
| XmaJI | CCTAGG | 1 cut(s) | 286 |
| XspI | CTAG | 2 cut(s) | 202, 287 |