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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 450 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 173 |
| AccB1I | GGYRCC | 1 cut(s) | 40 |
| AccII | CGCG | 1 cut(s) | 372 |
| AciI | CCGC | 5 cut(s) | 48, 370, 508, 603, 627 |
| AcyI | GRCGYC | 4 cut(s) | 41, 225, 392, 491 |
| AdeI | CACNNNGTG | 1 cut(s) | 345 |
| AfaI | GTAC | 1 cut(s) | 125 |
| AgsI | TTSAA | 4 cut(s) | 193, 297, 406, 515 |
| AjnI | CCWGG | 1 cut(s) | 75 |
| AluBI | AGCT | 1 cut(s) | 467 |
| AluI | AGCT | 1 cut(s) | 467 |
| AoxI | GGCC | 1 cut(s) | 425 |
| ApeKI | GCWGC | 2 cut(s) | 464, 621 |
| AspLEI | GCGC | 3 cut(s) | 43, 355, 411 |
| AspS9I | GGNCC | 1 cut(s) | 425 |
| AsuC2I | CCSGG | 1 cut(s) | 119 |
| AsuHPI | GGTGA | 3 cut(s) | 193, 331, 431 |
| BanI | GGYRCC | 1 cut(s) | 40 |
| BbvI | GCAGC | 2 cut(s) | 476, 608 |
| BccI | CCATC | 1 cut(s) | 119 |
| BceAI | ACGGC | 2 cut(s) | 80, 169 |
| BciT130I | CCWGG | 1 cut(s) | 77 |
| BcnI | CCSGG | 1 cut(s) | 119 |
| BfaI | CTAG | 1 cut(s) | 11 |
| BfoI | RGCGCY | 2 cut(s) | 44, 356 |
| BisI | GCNGC | 3 cut(s) | 370, 465, 622 |
| BlsI | GCNGC | 3 cut(s) | 371, 466, 623 |
| Bme1390I | CCNGG | 2 cut(s) | 77, 119 |
| BmgT120I | GGNCC | 1 cut(s) | 425 |
| BmiI | GGNNCC | 2 cut(s) | 42, 426 |
| BmrFI | CCNGG | 2 cut(s) | 77, 119 |
| BmsI | GCATC | 1 cut(s) | 623 |
| BpuEI | CTTGAG | 2 cut(s) | 286, 404 |
| BpuMI | CCSGG | 1 cut(s) | 119 |
| Bsa29I | ATCGAT | 1 cut(s) | 634 |
| BsaHI | GRCGYC | 4 cut(s) | 41, 225, 392, 491 |
| Bse118I | RCCGGY | 1 cut(s) | 394 |
| BseBI | CCWGG | 1 cut(s) | 77 |
| BseCI | ATCGAT | 1 cut(s) | 634 |
| BseGI | GGATG | 4 cut(s) | 111, 359, 454, 494 |
| BseRI | GAGGAG | 1 cut(s) | 407 |
| BseXI | GCAGC | 2 cut(s) | 476, 608 |
| BsgI | GTGCAG | 1 cut(s) | 607 |
| Bsh1236I | CGCG | 1 cut(s) | 372 |
| BshFI | GGCC | 1 cut(s) | 427 |
| BshNI | GGYRCC | 1 cut(s) | 40 |
| BshVI | ATCGAT | 1 cut(s) | 634 |
| BsiSI | CCGG | 4 cut(s) | 119, 258, 269, 395 |
| BsiWI | CGTACG | 1 cut(s) | 123 |
| BsnI | GGCC | 1 cut(s) | 427 |
| Bsp143I | GATC | 2 cut(s) | 162, 373 |
| BspACI | CCGC | 5 cut(s) | 48, 370, 508, 603, 627 |
| BspANI | GGCC | 1 cut(s) | 427 |
| BspDI | ATCGAT | 1 cut(s) | 634 |
| BspFNI | CGCG | 1 cut(s) | 372 |
| BspHI | TCATGA | 1 cut(s) | 480 |
| BspLI | GGNNCC | 2 cut(s) | 42, 426 |
| BspT107I | GGYRCC | 1 cut(s) | 40 |
| BsrFI | RCCGGY | 1 cut(s) | 394 |
| BssAI | RCCGGY | 1 cut(s) | 394 |
| BssMI | GATC | 2 cut(s) | 162, 373 |
| BssNI | GRCGYC | 4 cut(s) | 41, 225, 392, 491 |
| Bst2UI | CCWGG | 1 cut(s) | 77 |
| Bst4CI | ACNGT | 1 cut(s) | 343 |
| Bst6I | CTCTTC | 1 cut(s) | 72 |
| BstACI | GRCGYC | 4 cut(s) | 41, 225, 392, 491 |
| BstC8I | GCNNGC | 3 cut(s) | 39, 351, 396 |
| BstF5I | GGATG | 4 cut(s) | 111, 359, 454, 494 |
| BstFNI | CGCG | 1 cut(s) | 372 |
| BstH2I | RGCGCY | 2 cut(s) | 44, 356 |
| BstHHI | GCGC | 3 cut(s) | 43, 355, 411 |
| BstKTI | GATC | 2 cut(s) | 165, 376 |
| BstMBI | GATC | 2 cut(s) | 162, 373 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 47, 611 |
| BstNI | CCWGG | 1 cut(s) | 77 |
| BstNSI | RCATGY | 1 cut(s) | 110 |
| BstSCI | CCNGG | 2 cut(s) | 75, 117 |
| BstUI | CGCG | 1 cut(s) | 372 |
| BstV1I | GCAGC | 2 cut(s) | 476, 608 |
| Bsu15I | ATCGAT | 1 cut(s) | 634 |
| BsuRI | GGCC | 1 cut(s) | 427 |
| BsuTUI | ATCGAT | 1 cut(s) | 634 |
| BtgZI | GCGATG | 1 cut(s) | 95 |
| BtsCI | GGATG | 4 cut(s) | 111, 359, 454, 494 |
| Cac8I | GCNNGC | 3 cut(s) | 39, 351, 396 |
| CciI | TCATGA | 1 cut(s) | 480 |
| CfoI | GCGC | 3 cut(s) | 43, 355, 411 |
| Cfr10I | RCCGGY | 1 cut(s) | 394 |
| Cfr13I | GGNCC | 1 cut(s) | 425 |
| ClaI | ATCGAT | 1 cut(s) | 634 |
| CseI | GACGC | 3 cut(s) | 233, 400, 480 |
| Csp6I | GTAC | 1 cut(s) | 124 |
| CviAII | CATG | 5 cut(s) | 107, 160, 476, 481, 499 |
| CviJI | RGCY | 3 cut(s) | 22, 427, 467 |
| CviKI_1 | RGCY | 3 cut(s) | 22, 427, 467 |
| CviQI | GTAC | 1 cut(s) | 124 |
| DinI | GGCGCC | 1 cut(s) | 42 |
| DpnI | GATC | 2 cut(s) | 164, 375 |
| DpnII | GATC | 2 cut(s) | 162, 373 |
| DraIII | CACNNNGTG | 1 cut(s) | 345 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 173 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 173 |
| Eam1104I | CTCTTC | 1 cut(s) | 72 |
| EarI | CTCTTC | 1 cut(s) | 72 |
| EcoRII | CCWGG | 1 cut(s) | 75 |
| EgeI | GGCGCC | 1 cut(s) | 42 |
| EheI | GGCGCC | 1 cut(s) | 42 |
| FaeI | CATG | 5 cut(s) | 110, 163, 479, 484, 502 |
| FatI | CATG | 5 cut(s) | 106, 159, 475, 480, 498 |
| FauI | CCCGC | 1 cut(s) | 55 |
| FauNDI | CATATG | 2 cut(s) | 180, 521 |
| Fnu4HI | GCNGC | 3 cut(s) | 370, 465, 622 |
| FokI | GGATG | 4 cut(s) | 98, 346, 441, 481 |
| Fsp4HI | GCNGC | 3 cut(s) | 370, 465, 622 |
| FspBI | CTAG | 1 cut(s) | 11 |
| GlaI | GCGC | 3 cut(s) | 42, 354, 410 |
| GluI | GCNGC | 3 cut(s) | 370, 465, 622 |
| HaeII | RGCGCY | 2 cut(s) | 44, 356 |
| HaeIII | GGCC | 1 cut(s) | 427 |
| HapII | CCGG | 4 cut(s) | 119, 258, 269, 395 |
| HgaI | GACGC | 3 cut(s) | 233, 400, 480 |
| HhaI | GCGC | 3 cut(s) | 43, 355, 411 |
| Hin1I | GRCGYC | 4 cut(s) | 41, 225, 392, 491 |
| Hin1II | CATG | 5 cut(s) | 110, 163, 479, 484, 502 |
| Hin6I | GCGC | 3 cut(s) | 41, 353, 409 |
| HinP1I | GCGC | 3 cut(s) | 41, 353, 409 |
| HinfI | GANTC | 2 cut(s) | 414, 511 |
| HpaII | CCGG | 4 cut(s) | 119, 258, 269, 395 |
| HphI | GGTGA | 3 cut(s) | 193, 331, 431 |
| Hpy166II | GTNNAC | 1 cut(s) | 574 |
| Hpy188III | TCNNGA | 2 cut(s) | 28, 481 |
| Hpy8I | GTNNAC | 1 cut(s) | 574 |
| HpyAV | CCTTC | 2 cut(s) | 199, 593 |
| HpyCH4III | ACNGT | 1 cut(s) | 343 |
| HpyCH4V | TGCA | 4 cut(s) | 4, 464, 502, 624 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 47, 611 |
| Hsp92I | GRCGYC | 4 cut(s) | 41, 225, 392, 491 |
| Hsp92II | CATG | 5 cut(s) | 110, 163, 479, 484, 502 |
| HspAI | GCGC | 3 cut(s) | 41, 353, 409 |
| KasI | GGCGCC | 1 cut(s) | 40 |
| KroI | GCCGGC | 1 cut(s) | 394 |
| KroNI | GCCGGC | 1 cut(s) | 396 |
| Kzo9I | GATC | 2 cut(s) | 162, 373 |
| LpnPI | CCDG | 7 cut(s) | 23, 62, 89, 132, 271, 282, 408 |
| Lsp1109I | GCAGC | 2 cut(s) | 476, 608 |
| LweI | GCATC | 1 cut(s) | 623 |
| MaeI | CTAG | 1 cut(s) | 11 |
| MaeIII | GTNAC | 3 cut(s) | 144, 199, 337 |
| MalI | GATC | 2 cut(s) | 164, 375 |
| MboI | GATC | 2 cut(s) | 162, 373 |
| MboII | GAAGA | 1 cut(s) | 59 |
| MluCI | AATT | 1 cut(s) | 445 |
| Mly113I | GGCGCC | 1 cut(s) | 41 |
| MlyI | GAGTC | 1 cut(s) | 408 |
| MnlI | CCTC | 8 cut(s) | 27, 242, 301, 310, 310, 428, 452, 481 |
| MreI | CGCCGGCG | 1 cut(s) | 394 |
| MroNI | GCCGGC | 1 cut(s) | 394 |
| MseI | TTAA | 2 cut(s) | 326, 378 |
| MslI | CAYNNNNRTG | 2 cut(s) | 459, 594 |
| MspI | CCGG | 4 cut(s) | 119, 258, 269, 395 |
| MspR9I | CCNGG | 2 cut(s) | 77, 119 |
| MvaI | CCWGG | 1 cut(s) | 77 |
| MvnI | CGCG | 1 cut(s) | 372 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 47, 611 |
| NaeI | GCCGGC | 1 cut(s) | 396 |
| NarI | GGCGCC | 1 cut(s) | 41 |
| NciI | CCSGG | 1 cut(s) | 119 |
| NdeI | CATATG | 2 cut(s) | 180, 521 |
| NdeII | GATC | 2 cut(s) | 162, 373 |
| NgoMIV | GCCGGC | 1 cut(s) | 394 |
| NlaIII | CATG | 5 cut(s) | 110, 163, 479, 484, 502 |
| NlaIV | GGNNCC | 2 cut(s) | 42, 426 |
| NmuCI | GTSAC | 3 cut(s) | 144, 199, 337 |
| NspI | RCATGY | 1 cut(s) | 110 |
| PagI | TCATGA | 1 cut(s) | 480 |
| PdiI | GCCGGC | 1 cut(s) | 396 |
| PfeI | GAWTC | 1 cut(s) | 511 |
| Pfl23II | CGTACG | 1 cut(s) | 123 |
| PflFI | GACNNNGTC | 1 cut(s) | 476 |
| PkrI | GCNGC | 3 cut(s) | 371, 466, 623 |
| PleI | GAGTC | 1 cut(s) | 408 |
| PluTI | GGCGCC | 1 cut(s) | 44 |
| PpsI | GAGTC | 1 cut(s) | 408 |
| PsiI | TTATAA | 1 cut(s) | 450 |
| Psp6I | CCWGG | 1 cut(s) | 75 |
| PspGI | CCWGG | 1 cut(s) | 75 |
| PspLI | CGTACG | 1 cut(s) | 123 |
| PspN4I | GGNNCC | 2 cut(s) | 42, 426 |
| PspPI | GGNCC | 1 cut(s) | 425 |
| PsyI | GACNNNGTC | 1 cut(s) | 476 |
| RsaI | GTAC | 1 cut(s) | 125 |
| RsaNI | GTAC | 1 cut(s) | 124 |
| RseI | CAYNNNNRTG | 2 cut(s) | 459, 594 |
| SaqAI | TTAA | 2 cut(s) | 326, 378 |
| SatI | GCNGC | 3 cut(s) | 370, 465, 622 |
| Sau3AI | GATC | 2 cut(s) | 162, 373 |
| Sau96I | GGNCC | 1 cut(s) | 425 |
| SchI | GAGTC | 1 cut(s) | 408 |
| ScrFI | CCNGG | 2 cut(s) | 77, 119 |
| SetI | ASST | 5 cut(s) | 191, 302, 444, 469, 566 |
| SfaNI | GCATC | 1 cut(s) | 623 |
| SfoI | GGCGCC | 1 cut(s) | 42 |
| SgrAI | CRCCGGYG | 1 cut(s) | 394 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 459, 594 |
| SmlI | CTYRAG | 2 cut(s) | 301, 419 |
| SmoI | CTYRAG | 2 cut(s) | 301, 419 |
| Sse9I | AATT | 1 cut(s) | 445 |
| SsiI | CCGC | 5 cut(s) | 48, 370, 508, 603, 627 |
| SspDI | GGCGCC | 1 cut(s) | 40 |
| SspMI | CTAG | 1 cut(s) | 11 |
| StyD4I | CCNGG | 2 cut(s) | 75, 117 |
| TaaI | ACNGT | 1 cut(s) | 343 |
| TaqI | TCGA | 2 cut(s) | 165, 634 |
| TasI | AATT | 1 cut(s) | 445 |
| TauI | GCSGC | 1 cut(s) | 372 |
| TfiI | GAWTC | 1 cut(s) | 511 |
| Tru1I | TTAA | 2 cut(s) | 326, 378 |
| Tru9I | TTAA | 2 cut(s) | 326, 378 |
| TseFI | GTSAC | 3 cut(s) | 144, 199, 337 |
| TseI | GCWGC | 2 cut(s) | 464, 621 |
| Tsp45I | GTSAC | 3 cut(s) | 144, 199, 337 |
| TspDTI | ATGAA | 1 cut(s) | 612 |
| Tth111I | GACNNNGTC | 1 cut(s) | 476 |
| XceI | RCATGY | 1 cut(s) | 110 |
| XspI | CTAG | 1 cut(s) | 11 |