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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 247 |
| Acc36I | ACCTGC | 3 cut(s) | 25, 247, 394 |
| Acc65I | GGTACC | 1 cut(s) | 365 |
| AccB1I | GGYRCC | 2 cut(s) | 344, 365 |
| AccII | CGCG | 4 cut(s) | 90, 92, 219, 325 |
| AclWI | GGATC | 2 cut(s) | 201, 536 |
| AcoI | YGGCCR | 1 cut(s) | 261 |
| AfaI | GTAC | 2 cut(s) | 367, 396 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 151, 256, 523, 524, 525 |
| AhlI | ACTAGT | 1 cut(s) | 5 |
| AjnI | CCWGG | 3 cut(s) | 347, 361, 519 |
| AluBI | AGCT | 2 cut(s) | 289, 427 |
| AluI | AGCT | 2 cut(s) | 289, 427 |
| Alw26I | GTCTC | 2 cut(s) | 119, 561 |
| AlwI | GGATC | 2 cut(s) | 201, 536 |
| Ama87I | CYCGRG | 2 cut(s) | 151, 505 |
| AoxI | GGCC | 6 cut(s) | 154, 240, 246, 261, 440, 509 |
| ApeKI | GCWGC | 3 cut(s) | 138, 141, 412 |
| Asp718I | GGTACC | 1 cut(s) | 365 |
| AspLEI | GCGC | 4 cut(s) | 92, 219, 325, 417 |
| AspS9I | GGNCC | 4 cut(s) | 154, 247, 406, 440 |
| AsuC2I | CCSGG | 4 cut(s) | 117, 152, 153, 320 |
| AsuHPI | GGTGA | 2 cut(s) | 91, 134 |
| AsuNHI | GCTAGC | 1 cut(s) | 427 |
| AvaI | CYCGRG | 2 cut(s) | 151, 505 |
| AvaII | GGWCC | 1 cut(s) | 406 |
| BaeGI | GKGCMC | 2 cut(s) | 347, 456 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 255, 288 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| BanI | GGYRCC | 2 cut(s) | 344, 365 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 3 cut(s) | 150, 153, 399 |
| BccI | CCATC | 1 cut(s) | 507 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 482, 516 |
| BciT130I | CCWGG | 3 cut(s) | 349, 363, 521 |
| BciVI | GTATCC | 1 cut(s) | 536 |
| BcnI | CCSGG | 4 cut(s) | 117, 152, 153, 320 |
| BcoDI | GTCTC | 2 cut(s) | 119, 561 |
| BcuI | ACTAGT | 1 cut(s) | 5 |
| BfaI | CTAG | 4 cut(s) | 6, 302, 428, 513 |
| BfuAI | ACCTGC | 3 cut(s) | 25, 247, 394 |
| BfuI | GTATCC | 1 cut(s) | 536 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 320 |
| BisI | GCNGC | 5 cut(s) | 139, 142, 145, 413, 443 |
| BlsI | GCNGC | 5 cut(s) | 140, 143, 146, 414, 444 |
| Bme1390I | CCNGG | 7 cut(s) | 117, 152, 153, 320, 349, 363, 521 |
| Bme18I | GGWCC | 1 cut(s) | 406 |
| BmeT110I | CYCGRG | 2 cut(s) | 151, 505 |
| BmgT120I | GGNCC | 4 cut(s) | 154, 247, 406, 440 |
| BmiI | GGNNCC | 3 cut(s) | 346, 367, 408 |
| BmrFI | CCNGG | 7 cut(s) | 117, 152, 153, 320, 349, 363, 521 |
| BmrI | ACTGGG | 1 cut(s) | 101 |
| BmsI | GCATC | 1 cut(s) | 90 |
| BmtI | GCTAGC | 1 cut(s) | 431 |
| BmuI | ACTGGG | 1 cut(s) | 101 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 4 cut(s) | 117, 152, 153, 320 |
| BsaJI | CCNNGG | 4 cut(s) | 115, 151, 243, 519 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 467, 497 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 151, 256, 523, 524, 525 |
| Bse118I | RCCGGY | 1 cut(s) | 561 |
| Bse1I | ACTGG | 3 cut(s) | 107, 192, 389 |
| Bse3DI | GCAATG | 1 cut(s) | 162 |
| BseBI | CCWGG | 3 cut(s) | 349, 363, 521 |
| BseDI | CCNNGG | 4 cut(s) | 115, 151, 243, 519 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 151, 256, 523, 524, 525 |
| BseMI | GCAATG | 1 cut(s) | 162 |
| BseMII | CTCAG | 2 cut(s) | 18, 543 |
| BseNI | ACTGG | 3 cut(s) | 107, 192, 389 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseSI | GKGCMC | 2 cut(s) | 347, 456 |
| BseXI | GCAGC | 3 cut(s) | 150, 153, 399 |
| BseYI | CCCAGC | 1 cut(s) | 70 |
| BsgI | GTGCAG | 1 cut(s) | 476 |
| Bsh1236I | CGCG | 4 cut(s) | 90, 92, 219, 325 |
| BshFI | GGCC | 6 cut(s) | 156, 242, 248, 263, 442, 511 |
| BshNI | GGYRCC | 2 cut(s) | 344, 365 |
| BsiHKCI | CYCGRG | 2 cut(s) | 151, 505 |
| BsiSI | CCGG | 5 cut(s) | 117, 152, 320, 422, 562 |
| BslFI | GGGAC | 2 cut(s) | 198, 392 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 151, 256, 523, 524, 525 |
| BsmAI | GTCTC | 2 cut(s) | 119, 561 |
| BsmFI | GGGAC | 2 cut(s) | 198, 392 |
| BsnI | GGCC | 6 cut(s) | 156, 242, 248, 263, 442, 511 |
| BsoBI | CYCGRG | 2 cut(s) | 151, 505 |
| Bsp1286I | GDGCHC | 2 cut(s) | 347, 456 |
| Bsp143I | GATC | 2 cut(s) | 193, 528 |
| BspANI | GGCC | 6 cut(s) | 156, 242, 248, 263, 442, 511 |
| BspCNI | CTCAG | 2 cut(s) | 19, 544 |
| BspFNI | CGCG | 4 cut(s) | 90, 92, 219, 325 |
| BspLI | GGNNCC | 3 cut(s) | 346, 367, 408 |
| BspMI | ACCTGC | 3 cut(s) | 25, 247, 394 |
| BspOI | GCTAGC | 1 cut(s) | 431 |
| BspPI | GGATC | 2 cut(s) | 201, 536 |
| BspT107I | GGYRCC | 2 cut(s) | 344, 365 |
| BsrDI | GCAATG | 1 cut(s) | 162 |
| BsrFI | RCCGGY | 1 cut(s) | 561 |
| BsrI | ACTGG | 3 cut(s) | 107, 192, 389 |
| BssAI | RCCGGY | 1 cut(s) | 561 |
| BssECI | CCNNGG | 4 cut(s) | 115, 151, 243, 519 |
| BssMI | GATC | 2 cut(s) | 193, 528 |
| Bst2UI | CCWGG | 3 cut(s) | 349, 363, 521 |
| BstC8I | GCNNGC | 4 cut(s) | 221, 332, 419, 429 |
| BstDEI | CTNAG | 2 cut(s) | 27, 552 |
| BstFNI | CGCG | 4 cut(s) | 90, 92, 219, 325 |
| BstHHI | GCGC | 4 cut(s) | 92, 219, 325, 417 |
| BstKTI | GATC | 2 cut(s) | 196, 531 |
| BstMAI | GTCTC | 2 cut(s) | 119, 561 |
| BstMBI | GATC | 2 cut(s) | 193, 528 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 40, 87, 144, 153, 320, 331, 409, 418, 451 |
| BstNI | CCWGG | 3 cut(s) | 349, 363, 521 |
| BstNSI | RCATGY | 1 cut(s) | 403 |
| BstSCI | CCNGG | 7 cut(s) | 115, 150, 151, 318, 347, 361, 519 |
| BstSLI | GKGCMC | 2 cut(s) | 347, 456 |
| BstUI | CGCG | 4 cut(s) | 90, 92, 219, 325 |
| BstV1I | GCAGC | 3 cut(s) | 150, 153, 399 |
| BstX2I | RGATCY | 1 cut(s) | 193 |
| BstYI | RGATCY | 1 cut(s) | 193 |
| BsuI | GTATCC | 1 cut(s) | 536 |
| BsuRI | GGCC | 6 cut(s) | 156, 242, 248, 263, 442, 511 |
| BtgZI | GCGATG | 1 cut(s) | 485 |
| BtsI | GCAGTG | 1 cut(s) | 488 |
| BtsIMutI | CAGTG | 1 cut(s) | 488 |
| BveI | ACCTGC | 3 cut(s) | 25, 247, 394 |
| Cac8I | GCNNGC | 4 cut(s) | 221, 332, 419, 429 |
| CfoI | GCGC | 4 cut(s) | 92, 219, 325, 417 |
| Cfr10I | RCCGGY | 1 cut(s) | 561 |
| Cfr13I | GGNCC | 4 cut(s) | 154, 247, 406, 440 |
| Cfr9I | CCCGGG | 1 cut(s) | 151 |
| CsiI | ACCWGGT | 1 cut(s) | 361 |
| Csp6I | GTAC | 2 cut(s) | 366, 395 |
| CviAII | CATG | 1 cut(s) | 400 |
| CviQI | GTAC | 2 cut(s) | 366, 395 |
| DdeI | CTNAG | 2 cut(s) | 27, 552 |
| DpnI | GATC | 2 cut(s) | 195, 530 |
| DpnII | GATC | 2 cut(s) | 193, 528 |
| EaeI | YGGCCR | 1 cut(s) | 261 |
| EciI | GGCGGA | 1 cut(s) | 206 |
| Eco147I | AGGCCT | 1 cut(s) | 511 |
| Eco47I | GGWCC | 1 cut(s) | 406 |
| Eco88I | CYCGRG | 2 cut(s) | 151, 505 |
| EcoO109I | RGGNCCY | 1 cut(s) | 406 |
| EcoRII | CCWGG | 3 cut(s) | 347, 361, 519 |
| FaeI | CATG | 1 cut(s) | 403 |
| FaiI | YATR | 3 cut(s) | 175, 177, 401 |
| FaqI | GGGAC | 2 cut(s) | 198, 392 |
| FatI | CATG | 1 cut(s) | 399 |
| FauI | CCCGC | 4 cut(s) | 417, 426, 517, 532 |
| Fnu4HI | GCNGC | 5 cut(s) | 139, 142, 145, 413, 443 |
| Fsp4HI | GCNGC | 5 cut(s) | 139, 142, 145, 413, 443 |
| FspBI | CTAG | 4 cut(s) | 6, 302, 428, 513 |
| GlaI | GCGC | 4 cut(s) | 91, 218, 324, 416 |
| GluI | GCNGC | 5 cut(s) | 139, 142, 145, 413, 443 |
| GsaI | CCCAGC | 1 cut(s) | 74 |
| HaeIII | GGCC | 6 cut(s) | 156, 242, 248, 263, 442, 511 |
| HapII | CCGG | 5 cut(s) | 117, 152, 320, 422, 562 |
| HhaI | GCGC | 4 cut(s) | 92, 219, 325, 417 |
| Hin1II | CATG | 1 cut(s) | 403 |
| Hin6I | GCGC | 4 cut(s) | 90, 217, 323, 415 |
| HinP1I | GCGC | 4 cut(s) | 90, 217, 323, 415 |
| HincII | GTYRAC | 1 cut(s) | 112 |
| HindII | GTYRAC | 1 cut(s) | 112 |
| HinfI | GANTC | 1 cut(s) | 554 |
| HpaII | CCGG | 5 cut(s) | 117, 152, 320, 422, 562 |
| HphI | GGTGA | 2 cut(s) | 91, 134 |
| Hpy166II | GTNNAC | 3 cut(s) | 99, 112, 397 |
| Hpy188I | TCNGA | 1 cut(s) | 553 |
| Hpy8I | GTNNAC | 3 cut(s) | 99, 112, 397 |
| HpyAV | CCTTC | 1 cut(s) | 558 |
| HpyCH4V | TGCA | 2 cut(s) | 403, 493 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 40, 87, 144, 153, 320, 331, 409, 418, 451 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 552 |
| Hsp92II | CATG | 1 cut(s) | 403 |
| HspAI | GCGC | 4 cut(s) | 90, 217, 323, 415 |
| KpnI | GGTACC | 1 cut(s) | 369 |
| Kzo9I | GATC | 2 cut(s) | 193, 528 |
| LmnI | GCTCC | 3 cut(s) | 31, 327, 424 |
| Lsp1109I | GCAGC | 3 cut(s) | 150, 153, 399 |
| LweI | GCATC | 1 cut(s) | 90 |
| MabI | ACCWGGT | 1 cut(s) | 361 |
| MaeI | CTAG | 4 cut(s) | 6, 302, 428, 513 |
| MaeIII | GTNAC | 1 cut(s) | 336 |
| MalI | GATC | 2 cut(s) | 195, 530 |
| MboI | GATC | 2 cut(s) | 193, 528 |
| MboII | GAAGA | 1 cut(s) | 188 |
| MflI | RGATCY | 1 cut(s) | 193 |
| MhlI | GDGCHC | 2 cut(s) | 347, 456 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluCI | AATT | 1 cut(s) | 435 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MlyI | GAGTC | 1 cut(s) | 563 |
| MnlI | CCTC | 6 cut(s) | 22, 158, 178, 238, 469, 501 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 3 cut(s) | 74, 412, 539 |
| MspI | CCGG | 5 cut(s) | 117, 152, 320, 422, 562 |
| MspR9I | CCNGG | 7 cut(s) | 117, 152, 153, 320, 349, 363, 521 |
| MvaI | CCWGG | 3 cut(s) | 349, 363, 521 |
| MvnI | CGCG | 4 cut(s) | 90, 92, 219, 325 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 40, 87, 144, 153, 320, 331, 409, 418, 451 |
| NciI | CCSGG | 4 cut(s) | 117, 152, 153, 320 |
| NdeII | GATC | 2 cut(s) | 193, 528 |
| NheI | GCTAGC | 1 cut(s) | 427 |
| NlaIII | CATG | 1 cut(s) | 403 |
| NlaIV | GGNNCC | 3 cut(s) | 346, 367, 408 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 1 cut(s) | 336 |
| NspI | RCATGY | 1 cut(s) | 403 |
| PaeR7I | CTCGAG | 1 cut(s) | 505 |
| PaqCI | CACCTGC | 1 cut(s) | 247 |
| PceI | AGGCCT | 1 cut(s) | 511 |
| PkrI | GCNGC | 5 cut(s) | 140, 143, 146, 414, 444 |
| PleI | GAGTC | 1 cut(s) | 562 |
| PpsI | GAGTC | 1 cut(s) | 562 |
| PpuMI | RGGWCCY | 1 cut(s) | 406 |
| Psp5II | RGGWCCY | 1 cut(s) | 406 |
| Psp6I | CCWGG | 3 cut(s) | 347, 361, 519 |
| PspFI | CCCAGC | 1 cut(s) | 70 |
| PspGI | CCWGG | 3 cut(s) | 347, 361, 519 |
| PspN4I | GGNNCC | 3 cut(s) | 346, 367, 408 |
| PspPI | GGNCC | 4 cut(s) | 154, 247, 406, 440 |
| PspPPI | RGGWCCY | 1 cut(s) | 406 |
| PspXI | VCTCGAGB | 1 cut(s) | 505 |
| PsuI | RGATCY | 1 cut(s) | 193 |
| RsaI | GTAC | 2 cut(s) | 367, 396 |
| RsaNI | GTAC | 2 cut(s) | 366, 395 |
| SatI | GCNGC | 5 cut(s) | 139, 142, 145, 413, 443 |
| Sau3AI | GATC | 2 cut(s) | 193, 528 |
| Sau96I | GGNCC | 4 cut(s) | 154, 247, 406, 440 |
| SchI | GAGTC | 1 cut(s) | 563 |
| ScrFI | CCNGG | 7 cut(s) | 117, 152, 153, 320, 349, 363, 521 |
| SduI | GDGCHC | 2 cut(s) | 347, 456 |
| SetI | ASST | 7 cut(s) | 39, 261, 281, 291, 364, 408, 429 |
| SexAI | ACCWGGT | 1 cut(s) | 361 |
| SfaNI | GCATC | 1 cut(s) | 90 |
| Sfr274I | CTCGAG | 1 cut(s) | 505 |
| SgrAI | CRCCGGYG | 1 cut(s) | 561 |
| SinI | GGWCC | 1 cut(s) | 406 |
| SlaI | CTCGAG | 1 cut(s) | 505 |
| SmaI | CCCGGG | 1 cut(s) | 153 |
| SmlI | CTYRAG | 1 cut(s) | 505 |
| SmoI | CTYRAG | 1 cut(s) | 505 |
| SpeI | ACTAGT | 1 cut(s) | 5 |
| Sse9I | AATT | 1 cut(s) | 435 |
| SseBI | AGGCCT | 1 cut(s) | 511 |
| SspMI | CTAG | 4 cut(s) | 6, 302, 428, 513 |
| StuI | AGGCCT | 1 cut(s) | 511 |
| StyD4I | CCNGG | 7 cut(s) | 115, 150, 151, 318, 347, 361, 519 |
| TaqI | TCGA | 1 cut(s) | 506 |
| TasI | AATT | 1 cut(s) | 435 |
| TauI | GCSGC | 2 cut(s) | 147, 445 |
| TscAI | CASTG | 1 cut(s) | 495 |
| TseFI | GTSAC | 1 cut(s) | 336 |
| TseI | GCWGC | 3 cut(s) | 138, 141, 412 |
| Tsp45I | GTSAC | 1 cut(s) | 336 |
| TspMI | CCCGGG | 1 cut(s) | 151 |
| TspRI | CASTG | 1 cut(s) | 495 |
| VpaK11BI | GGWCC | 1 cut(s) | 406 |
| XceI | RCATGY | 1 cut(s) | 403 |
| XhoI | CTCGAG | 1 cut(s) | 505 |
| XmaI | CCCGGG | 1 cut(s) | 151 |
| XspI | CTAG | 4 cut(s) | 6, 302, 428, 513 |