No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 38, 245 |
| AciI | CCGC | 7 cut(s) | 12, 19, 55, 101, 130, 171, 217 |
| AclI | AACGTT | 1 cut(s) | 44 |
| AclWI | GGATC | 1 cut(s) | 227 |
| AcoI | YGGCCR | 1 cut(s) | 287 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 102 |
| AgsI | TTSAA | 3 cut(s) | 81, 309, 318 |
| AjnI | CCWGG | 3 cut(s) | 159, 177, 182 |
| Alw21I | GWGCWC | 1 cut(s) | 75 |
| Alw44I | GTGCAC | 1 cut(s) | 71 |
| AlwI | GGATC | 1 cut(s) | 227 |
| Ama87I | CYCGRG | 1 cut(s) | 122 |
| AoxI | GGCC | 4 cut(s) | 180, 266, 272, 287 |
| ApaLI | GTGCAC | 1 cut(s) | 71 |
| AspLEI | GCGC | 3 cut(s) | 245, 327, 351 |
| AspS9I | GGNCC | 3 cut(s) | 126, 186, 273 |
| AsuC2I | CCSGG | 2 cut(s) | 123, 124 |
| AsuHPI | GGTGA | 1 cut(s) | 148 |
| AvaI | CYCGRG | 1 cut(s) | 122 |
| AvaII | GGWCC | 2 cut(s) | 126, 186 |
| BaeGI | GKGCMC | 1 cut(s) | 75 |
| BalI | TGGCCA | 1 cut(s) | 289 |
| Bbv12I | GWGCWC | 1 cut(s) | 75 |
| BccI | CCATC | 1 cut(s) | 30 |
| BciT130I | CCWGG | 3 cut(s) | 161, 179, 184 |
| BcnI | CCSGG | 2 cut(s) | 123, 124 |
| BfaI | CTAG | 2 cut(s) | 98, 328 |
| BfmI | CTRYAG | 1 cut(s) | 143 |
| BfoI | RGCGCY | 1 cut(s) | 328 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 179 |
| BisI | GCNGC | 1 cut(s) | 171 |
| BlsI | GCNGC | 1 cut(s) | 172 |
| Bme1390I | CCNGG | 5 cut(s) | 123, 124, 161, 179, 184 |
| Bme18I | GGWCC | 2 cut(s) | 126, 186 |
| BmeT110I | CYCGRG | 1 cut(s) | 122 |
| BmgT120I | GGNCC | 3 cut(s) | 126, 186, 273 |
| BmiI | GGNNCC | 3 cut(s) | 107, 127, 128 |
| BmrFI | CCNGG | 5 cut(s) | 123, 124, 161, 179, 184 |
| BpmI | CTGGAG | 1 cut(s) | 161 |
| BpuMI | CCSGG | 2 cut(s) | 123, 124 |
| BsaJI | CCNNGG | 4 cut(s) | 121, 122, 159, 269 |
| BsaWI | WCCGGW | 1 cut(s) | 87 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 102 |
| Bse1I | ACTGG | 1 cut(s) | 218 |
| BseBI | CCWGG | 3 cut(s) | 161, 179, 184 |
| BseDI | CCNNGG | 4 cut(s) | 121, 122, 159, 269 |
| BseGI | GGATG | 1 cut(s) | 22 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 102 |
| BseNI | ACTGG | 1 cut(s) | 218 |
| BseSI | GKGCMC | 1 cut(s) | 75 |
| BseYI | CCCAGC | 2 cut(s) | 51, 345 |
| Bsh1236I | CGCG | 2 cut(s) | 38, 245 |
| BshFI | GGCC | 4 cut(s) | 182, 268, 274, 289 |
| BsiHKAI | GWGCWC | 1 cut(s) | 75 |
| BsiHKCI | CYCGRG | 1 cut(s) | 122 |
| BsiSI | CCGG | 2 cut(s) | 88, 123 |
| BslFI | GGGAC | 1 cut(s) | 112 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 102 |
| BsmFI | GGGAC | 1 cut(s) | 112 |
| BsnI | GGCC | 4 cut(s) | 182, 268, 274, 289 |
| BsoBI | CYCGRG | 1 cut(s) | 122 |
| Bsp1286I | GDGCHC | 1 cut(s) | 75 |
| Bsp143I | GATC | 2 cut(s) | 4, 219 |
| BspACI | CCGC | 7 cut(s) | 12, 19, 55, 101, 130, 171, 217 |
| BspANI | GGCC | 4 cut(s) | 182, 268, 274, 289 |
| BspFNI | CGCG | 2 cut(s) | 38, 245 |
| BspLI | GGNNCC | 3 cut(s) | 107, 127, 128 |
| BspMAI | CTGCAG | 1 cut(s) | 147 |
| BspPI | GGATC | 1 cut(s) | 227 |
| BsrI | ACTGG | 1 cut(s) | 218 |
| BssECI | CCNNGG | 4 cut(s) | 121, 122, 159, 269 |
| BssMI | GATC | 2 cut(s) | 4, 219 |
| Bst2UI | CCWGG | 3 cut(s) | 161, 179, 184 |
| Bst4CI | ACNGT | 2 cut(s) | 70, 77 |
| BstC8I | GCNNGC | 1 cut(s) | 247 |
| BstF5I | GGATG | 1 cut(s) | 22 |
| BstFNI | CGCG | 2 cut(s) | 38, 245 |
| BstH2I | RGCGCY | 1 cut(s) | 328 |
| BstHHI | GCGC | 3 cut(s) | 245, 327, 351 |
| BstKTI | GATC | 2 cut(s) | 7, 222 |
| BstMBI | GATC | 2 cut(s) | 4, 219 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 151, 170, 179, 331, 346 |
| BstNI | CCWGG | 3 cut(s) | 161, 179, 184 |
| BstSCI | CCNGG | 5 cut(s) | 121, 122, 159, 177, 182 |
| BstSFI | CTRYAG | 1 cut(s) | 143 |
| BstSLI | GKGCMC | 1 cut(s) | 75 |
| BstUI | CGCG | 2 cut(s) | 38, 245 |
| BstX2I | RGATCY | 1 cut(s) | 219 |
| BstYI | RGATCY | 1 cut(s) | 219 |
| BsuRI | GGCC | 4 cut(s) | 182, 268, 274, 289 |
| BtsCI | GGATG | 1 cut(s) | 22 |
| BtsI | GCAGTG | 1 cut(s) | 152 |
| BtsIMutI | CAGTG | 4 cut(s) | 73, 152, 211, 351 |
| Cac8I | GCNNGC | 1 cut(s) | 247 |
| CfoI | GCGC | 3 cut(s) | 245, 327, 351 |
| Cfr13I | GGNCC | 3 cut(s) | 126, 186, 273 |
| Cfr9I | CCCGGG | 1 cut(s) | 122 |
| CviJI | RGCY | 6 cut(s) | 154, 170, 182, 268, 274, 289 |
| CviKI_1 | RGCY | 6 cut(s) | 154, 170, 182, 268, 274, 289 |
| DpnI | GATC | 2 cut(s) | 6, 221 |
| DpnII | GATC | 2 cut(s) | 4, 219 |
| EaeI | YGGCCR | 1 cut(s) | 287 |
| EciI | GGCGGA | 1 cut(s) | 232 |
| Eco47I | GGWCC | 2 cut(s) | 126, 186 |
| Eco88I | CYCGRG | 1 cut(s) | 122 |
| EcoO109I | RGGNCCY | 2 cut(s) | 126, 186 |
| EcoRII | CCWGG | 3 cut(s) | 159, 177, 182 |
| FaiI | YATR | 2 cut(s) | 199, 203 |
| FaqI | GGGAC | 1 cut(s) | 112 |
| FauI | CCCGC | 2 cut(s) | 94, 137 |
| Fnu4HI | GCNGC | 1 cut(s) | 171 |
| FokI | GGATG | 1 cut(s) | 9 |
| Fsp4HI | GCNGC | 1 cut(s) | 171 |
| FspBI | CTAG | 2 cut(s) | 98, 328 |
| GlaI | GCGC | 3 cut(s) | 244, 326, 350 |
| GluI | GCNGC | 1 cut(s) | 171 |
| GsaI | CCCAGC | 2 cut(s) | 55, 349 |
| GsuI | CTGGAG | 1 cut(s) | 161 |
| HaeII | RGCGCY | 1 cut(s) | 328 |
| HaeIII | GGCC | 4 cut(s) | 182, 268, 274, 289 |
| HapII | CCGG | 2 cut(s) | 88, 123 |
| HhaI | GCGC | 3 cut(s) | 245, 327, 351 |
| Hin6I | GCGC | 3 cut(s) | 243, 325, 349 |
| HinP1I | GCGC | 3 cut(s) | 243, 325, 349 |
| HincII | GTYRAC | 3 cut(s) | 48, 60, 93 |
| HindII | GTYRAC | 3 cut(s) | 48, 60, 93 |
| HpaI | GTTAAC | 2 cut(s) | 48, 60 |
| HpaII | CCGG | 2 cut(s) | 88, 123 |
| HphI | GGTGA | 1 cut(s) | 148 |
| Hpy166II | GTNNAC | 4 cut(s) | 48, 60, 73, 93 |
| Hpy8I | GTNNAC | 4 cut(s) | 48, 60, 73, 93 |
| HpyAV | CCTTC | 1 cut(s) | 315 |
| HpyCH4III | ACNGT | 2 cut(s) | 70, 77 |
| HpyCH4IV | ACGT | 2 cut(s) | 44, 292 |
| HpyCH4V | TGCA | 2 cut(s) | 73, 145 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 151, 170, 179, 331, 346 |
| HpySE526I | ACGT | 2 cut(s) | 44, 292 |
| HspAI | GCGC | 3 cut(s) | 243, 325, 349 |
| KflI | GGGWCCC | 1 cut(s) | 126 |
| KspAI | GTTAAC | 2 cut(s) | 48, 60 |
| Kzo9I | GATC | 2 cut(s) | 4, 219 |
| LmnI | GCTCC | 1 cut(s) | 240 |
| MaeI | CTAG | 2 cut(s) | 98, 328 |
| MaeII | ACGT | 2 cut(s) | 44, 292 |
| MalI | GATC | 2 cut(s) | 6, 221 |
| MboI | GATC | 2 cut(s) | 4, 219 |
| MboII | GAAGA | 1 cut(s) | 330 |
| MflI | RGATCY | 1 cut(s) | 219 |
| MhlI | GDGCHC | 1 cut(s) | 75 |
| MlsI | TGGCCA | 1 cut(s) | 289 |
| MluCI | AATT | 2 cut(s) | 234, 313 |
| MluNI | TGGCCA | 1 cut(s) | 289 |
| MnlI | CCTC | 4 cut(s) | 144, 185, 199, 264 |
| Mox20I | TGGCCA | 1 cut(s) | 289 |
| MscI | TGGCCA | 1 cut(s) | 289 |
| MseI | TTAA | 3 cut(s) | 47, 59, 65 |
| MslI | CAYNNNNRTG | 1 cut(s) | 295 |
| Msp20I | TGGCCA | 1 cut(s) | 289 |
| MspA1I | CMGCKG | 1 cut(s) | 55 |
| MspI | CCGG | 2 cut(s) | 88, 123 |
| MspR9I | CCNGG | 5 cut(s) | 123, 124, 161, 179, 184 |
| MvaI | CCWGG | 3 cut(s) | 161, 179, 184 |
| MvnI | CGCG | 2 cut(s) | 38, 245 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 151, 170, 179, 331, 346 |
| NciI | CCSGG | 2 cut(s) | 123, 124 |
| NdeII | GATC | 2 cut(s) | 4, 219 |
| NlaIV | GGNNCC | 3 cut(s) | 107, 127, 128 |
| NmeAIII | GCCGAG | 1 cut(s) | 294 |
| PkrI | GCNGC | 1 cut(s) | 172 |
| PpuMI | RGGWCCY | 2 cut(s) | 126, 186 |
| Psp1406I | AACGTT | 1 cut(s) | 44 |
| Psp5II | RGGWCCY | 2 cut(s) | 126, 186 |
| Psp6I | CCWGG | 3 cut(s) | 159, 177, 182 |
| PspFI | CCCAGC | 2 cut(s) | 51, 345 |
| PspGI | CCWGG | 3 cut(s) | 159, 177, 182 |
| PspN4I | GGNNCC | 3 cut(s) | 107, 127, 128 |
| PspPI | GGNCC | 3 cut(s) | 126, 186, 273 |
| PspPPI | RGGWCCY | 2 cut(s) | 126, 186 |
| PstI | CTGCAG | 1 cut(s) | 147 |
| PsuI | RGATCY | 1 cut(s) | 219 |
| RseI | CAYNNNNRTG | 1 cut(s) | 295 |
| SaqAI | TTAA | 3 cut(s) | 47, 59, 65 |
| SatI | GCNGC | 1 cut(s) | 171 |
| Sau3AI | GATC | 2 cut(s) | 4, 219 |
| Sau96I | GGNCC | 3 cut(s) | 126, 186, 273 |
| ScrFI | CCNGG | 5 cut(s) | 123, 124, 161, 179, 184 |
| SduI | GDGCHC | 1 cut(s) | 75 |
| SetI | ASST | 3 cut(s) | 47, 191, 295 |
| SfcI | CTRYAG | 1 cut(s) | 143 |
| SinI | GGWCC | 2 cut(s) | 126, 186 |
| SmaI | CCCGGG | 1 cut(s) | 124 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 295 |
| Sse9I | AATT | 2 cut(s) | 234, 313 |
| SsiI | CCGC | 7 cut(s) | 12, 19, 55, 101, 130, 171, 217 |
| SspMI | CTAG | 2 cut(s) | 98, 328 |
| StyD4I | CCNGG | 5 cut(s) | 121, 122, 159, 177, 182 |
| TaaI | ACNGT | 2 cut(s) | 70, 77 |
| TaiI | ACGT | 2 cut(s) | 47, 295 |
| TasI | AATT | 2 cut(s) | 234, 313 |
| TauI | GCSGC | 1 cut(s) | 173 |
| Tru1I | TTAA | 3 cut(s) | 47, 59, 65 |
| Tru9I | TTAA | 3 cut(s) | 47, 59, 65 |
| TscAI | CASTG | 3 cut(s) | 80, 152, 218 |
| TspDTI | ATGAA | 1 cut(s) | 326 |
| TspMI | CCCGGG | 1 cut(s) | 122 |
| TspRI | CASTG | 3 cut(s) | 80, 152, 218 |
| VneI | GTGCAC | 1 cut(s) | 71 |
| VpaK11BI | GGWCC | 2 cut(s) | 126, 186 |
| XmaI | CCCGGG | 1 cut(s) | 122 |
| XspI | CTAG | 2 cut(s) | 98, 328 |